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Frederick G. Gmitter - One of the best experts on this subject based on the ideXlab platform.
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Leaf area measurements of the allotetraploid somatic hybrids between OLL8 Sweet Orange and the finger lime and the diploid parents.
'Public Library of Science (PLoS)', 2021Co-Authors: Manjul Dutt, Frederick G. Gmitter, Lamiaa M. Mahmoud, Karen Chamusco, Daniel Stanton, Christine D. Chase, Ethan Nielsen, Maria Quirico, Jude W GrosserAbstract:Leaf area measurements of the allotetraploid somatic hybrids between OLL8 Sweet Orange and the finger lime and the diploid parents.
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Ct values of CaLas detected in the 6-year-old allotetraploid somatic hybrids between OLL8 Sweet Orange and the finger lime and the diploid parents.
'Public Library of Science (PLoS)', 2021Co-Authors: Manjul Dutt, Frederick G. Gmitter, Lamiaa M. Mahmoud, Karen Chamusco, Daniel Stanton, Christine D. Chase, Ethan Nielsen, Maria Quirico, Jude W GrosserAbstract:Ct values of CaLas detected in the 6-year-old allotetraploid somatic hybrids between OLL8 Sweet Orange and the finger lime and the diploid parents.
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Use of expressed sequence tag–simple sequence repeat (EST-SSR) to detect alleles and confirm allotetraploid somatic hybrids between OLL8 Sweet Orange and the finger lime (C. australasica).
'Public Library of Science (PLoS)', 2021Co-Authors: Manjul Dutt, Frederick G. Gmitter, Lamiaa M. Mahmoud, Karen Chamusco, Daniel Stanton, Christine D. Chase, Ethan Nielsen, Maria Quirico, Jude W GrosserAbstract:Use of expressed sequence tag–simple sequence repeat (EST-SSR) to detect alleles and confirm allotetraploid somatic hybrids between OLL8 Sweet Orange and the finger lime (C. australasica).
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differentiation between flavors of Sweet Orange citrus sinensis and mandarin citrus reticulata
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Shi Feng, Frederick G. Gmitter, Joon Hyuk Suh, Yu WangAbstract:Pioneering investigations referring to citrus flavor have been intensively conducted. However, the characteristic flavor difference between Sweet Orange and mandarin has not been defined. In this study, sensory analysis illustrated the crucial role of aroma in the differentiation between Orange flavor and mandarin flavor. To study aroma, Valencia Orange and LB8–9 mandarin were used. Their most aroma-active compounds were preliminarily identified by aroma extract dilution analysis (AEDA). Quantitation of key volatiles followed by calculation of odor activity values (OAVs) further detected potent components (OAV ≥ 1) impacting the overall aromatic profile of Orange/mandarin. Follow-up aroma profile analysis revealed that ethyl butanoate, ethyl 2-methylbutanoate, octanal, decanal, and acetaldehyde were essential for Orange-like aroma, whereas linalool, octanal, α-pinene, limonene, and (E,E)-2,4-decadienal were considered key components for mandarin-like aroma. Furthermore, an unreleased mandarin hybrid produ...
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Differentiation between Flavors of Sweet Orange (Citrus sinensis) and Mandarin (Citrus reticulata)
2017Co-Authors: Shi Feng, Frederick G. Gmitter, Joon Hyuk Suh, Yu WangAbstract:Pioneering investigations referring to citrus flavor have been intensively conducted. However, the characteristic flavor difference between Sweet Orange and mandarin has not been defined. In this study, sensory analysis illustrated the crucial role of aroma in the differentiation between Orange flavor and mandarin flavor. To study aroma, Valencia Orange and LB8–9 mandarin were used. Their most aroma-active compounds were preliminarily identified by aroma extract dilution analysis (AEDA). Quantitation of key volatiles followed by calculation of odor activity values (OAVs) further detected potent components (OAV ≥ 1) impacting the overall aromatic profile of Orange/mandarin. Follow-up aroma profile analysis revealed that ethyl butanoate, ethyl 2-methylbutanoate, octanal, decanal, and acetaldehyde were essential for Orange-like aroma, whereas linalool, octanal, α-pinene, limonene, and (E,E)-2,4-decadienal were considered key components for mandarin-like aroma. Furthermore, an unreleased mandarin hybrid producing fruit with Orange-like flavor was used to validate the identification of characteristic volatiles in Orange-like aroma
Xiuxin Deng - One of the best experts on this subject based on the ideXlab platform.
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fragile sites of valencia Sweet Orange citrus sinensis chromosomes are related with active 45s rdna
PLOS ONE, 2016Co-Authors: Hong Lan, Wenwu Guo, Chunli Chen, Yin Miao, Xiuxin DengAbstract:Citrus sinensis chromosomes present a morphological differentiation of bands after staining by the fluorochromes CMA and DAPI, but there is still little information on its chromosomal characteristics. In this study, the chromosomes in 'Valencia' C. sinensis were analyzed by fluorescence in situ hybridization (FISH) using telomere DNA and the 45S rDNA gene as probes combining CMA/DAPI staining, which showed that there were two fragile sites in Sweet Orange chromosomes co-localizing at distended 45S rDNA regions, one proximally locating on B-type chromosome and the other subterminally locating on D-type chromosome. While the chromosomal CMA banding and 45S rDNA FISH mapping in the doubled haploid line of 'Valencia' C. sinensis indicated six 45S rDNA regions, four were identified as fragile sites as doubled comparing its parental line, which confirmed the cytological heterozygosity and chromosomal heteromorphisms in Sweet Orange. Furthermore, Ag-NOR identified two distended 45S rDNA regions to be active nucleolar organizing regions (NORs) in diploid 'Valencia' C. sinensis. The occurrence of quadrivalent in meiosis of pollen mother cells (PMCs) in 'Valencia' Sweet Orange further confirmed it was a chromosomal reciprocal translocation line. We speculated this chromosome translocation was probably related to fragile sites. Our data provide insights into the chromosomal characteristics of the fragile sites in 'Valencia' Sweet Orange and are expected to facilitate the further investigation of the possible functions of fragile sites.
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genome wide comparison of micrornas and their targeted transcripts among leaf flower and fruit of Sweet Orange
BMC Genomics, 2014Co-Authors: Yuanlong Liu, Lingling Chen, Dijun Chen, Lun Wang, Ding Huang, Xiuxin DengAbstract:In plants, microRNAs (miRNAs) regulate gene expression mainly at the post-transcriptional level. Previous studies have demonstrated that miRNA-mediated gene silencing pathways play vital roles in plant development. Here, we used a high-throughput sequencing approach to characterize the miRNAs and their targeted transcripts in the leaf, flower and fruit of Sweet Orange. A total of 183 known miRNAs and 38 novel miRNAs were identified. An in-house script was used to identify all potential secondary siRNAs derived from miRNA-targeted transcripts using sRNA and degradome sequencing data. Genome mapping revealed that these miRNAs were evenly distributed across the genome with several small clusters, and 69 pre-miRNAs were co-localized with simple sequence repeats (SSRs). Noticeably, the loop size of pre-miR396c was influenced by the repeat number of CUU unit. The expression pattern of miRNAs among different tissues and developmental stages were further investigated by both qRT-PCR and RNA gel blotting. Interestingly, Csi-miR164 was highly expressed in fruit ripening stage, and was validated to target a NAC transcription factor. This study depicts a global picture of miRNAs and their target genes in the genome of Sweet Orange, and focused on the comparison among leaf, flower and fruit tissues. This study provides a global view of miRNAs and their target genes in different tissue of Sweet Orange, and focused on the identification of miRNA involved in the regulation of fruit ripening. The results of this study lay a foundation for unraveling key regulators of Orange fruit development and ripening on post-transcriptional level.
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transcriptome changes during fruit development and ripening of Sweet Orange citrus sinensis
BMC Genomics, 2012Co-Authors: Fei Guo, Yuduan Ding, Xiuxin DengAbstract:Background The transcriptome of the fruit pulp of the Sweet Orange variety Anliu (WT) and that of its red fleshed mutant Hong Anliu (MT) were compared to understand the dynamics and differential expression of genes expressed during fruit development and ripening.
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transcriptome changes during fruit development and ripening of Sweet Orange citrus sinensis
BMC Genomics, 2012Co-Authors: Fei Guo, Yuduan Ding, Xiuxin DengAbstract:The transcriptome of the fruit pulp of the Sweet Orange variety Anliu (WT) and that of its red fleshed mutant Hong Anliu (MT) were compared to understand the dynamics and differential expression of genes expressed during fruit development and ripening. The transcriptomes of WT and MT were sampled at four developmental stages using an Illumina sequencing platform. A total of 19,440 and 18,829 genes were detected in MT and WT, respectively. Hierarchical clustering analysis revealed 24 expression patterns for the set of all genes detected, of which 20 were in common between MT and WT. Over 89% of the genes showed differential expression during fruit development and ripening in the WT. Functional categorization of the differentially expressed genes revealed that cell wall biosynthesis, carbohydrate and citric acid metabolism, carotenoid metabolism, and the response to stress were the most differentially regulated processes occurring during fruit development and ripening. A description of the transcriptomic changes occurring during fruit development and ripening was obtained in Sweet Orange, along with a dynamic view of the gene expression differences between the wild type and a red fleshed mutant.
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A proteomic analysis of the chromoplasts isolated from Sweet Orange fruits [Citrus sinensis (L.) Osbeck]
Journal of Experimental Botany, 2011Co-Authors: Yunliu Zeng, Yuduan Ding, Qiang Xu, Xiuxin DengAbstract:Here, a comprehensive proteomic analysis of the chromoplasts purified from Sweet Orange using Nycodenz density gradient centrifugation is reported. A GeLC-MS/MS shotgun approach was used to identify the proteins of pooled chromoplast samples. A total of 493 proteins were identified from purified chromoplasts, of which 418 are putative plastid proteins based on in silico sequence homology and functional analyses. Based on the predicted functions of these identified plastid proteins, a large proportion (;60%) of the chromoplast proteome of Sweet Orange is constituted by proteins involved in carbohydrate metabolism, amino acid/protein synthesis, and secondary metabolism. Of note, HDS (hydroxymethylbutenyl 4-diphosphate synthase), PAP (plastid-lipid-associated protein), and psHSPs (plastid small heat shock proteins) involved in the synthesis or storage of carotenoid and stress response are among the most abundant proteins identified. A comparison of chromoplast proteomes between Sweet Orange and tomato suggested a high level of conservation in a broad range of metabolic pathways. However, the citrus chromoplast was characterized by more extensive carotenoid synthesis, extensive amino acid synthesis without nitrogen assimilation, and evidence for lipid metabolism concerning jasmonic acid synthesis. In conclusion, this study provides an insight into the major metabolic pathways as well as some unique characteristics of the Sweet Orange chromoplasts at the whole proteome level.
Chunxian Chen - One of the best experts on this subject based on the ideXlab platform.
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comparative transcriptional and anatomical analyses of tolerant rough lemon and susceptible Sweet Orange in response to candidatus liberibacter asiaticus infection
Molecular Plant-microbe Interactions, 2012Co-Authors: Jing Fan, Chunxian Chen, Diann Achor, Abeer Khalaf, Ronald H. Brlansky, Gloria A. Moore, Frederick G. GmitterAbstract:Although there are no known sources of genetic resistance, some Citrus spp. are reportedly tolerant to huanglongbing (HLB), presumably caused by ‘Candidatus Liberibacter asiaticus’. Time-course transcriptional analysis of tolerant rough lemon (Citrus jambhiri) and susceptible Sweet Orange (C. sinensis) in response to ‘Ca. L. asiaticus’ infection showed more genes differentially expressed in HLB-affected rough lemon than Sweet Orange at early stages but substantially fewer at late time points, possibly a critical factor underlying differences in sensitivity to ‘Ca. L. asiaticus’. Pathway analysis revealed that stress responses were distinctively modulated in rough lemon and Sweet Orange. Although microscopic changes (e.g., callose deposition in sieve elements and phloem cell collapse) were found in both infected species, remarkably, phloem transport activity in midribs of source leaves in rough lemon was much less affected by HLB than in Sweet Orange. The difference in phloem cell transport activities is a...
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comparative itraq proteome and transcriptome analyses of Sweet Orange infected by candidatus liberibacter asiaticus
Physiologia Plantarum, 2011Co-Authors: Jing Fan, Chunxian Chen, Ronald H. Brlansky, Frederick G. GmitterAbstract:Citrus Huanglongbing (HLB) has been threatening citrus production worldwide. In this study, a comparative proteomic approach was applied to understand the pathogenic process of HLB in affected Sweet Orange leaves. Using the isobaric tags for relative and absolute quantification (iTRAQ) technique, we identified 686 unique proteins in the mature leaves of both mock-inoculated and diseased 'Madam Vinous' Sweet Orange plants. Of the identified proteins, 20 and 10 were differentially expressed in leaves with and without symptoms of HLB (fold change > 2.5), respectively, compared with mock-inoculated controls. Most significantly, upregulated proteins were involved in stress/defense response, such as four miraculin-like proteins, chitinase, Cu/Zn superoxide dismutase and lipoxygenase. Microarray analysis also showed that stress-related genes were significantly upregulated at the transcriptional level. For example, remarkable upregulations of miraculin-like proteins and Cu/Zn superoxide dismutase transcripts were observed. Moreover, the transcriptional patterns of miraculin-like protein 1 and Cu/Zn superoxide dismutase were examined at different stages of HLB disease development. Combined with the transcriptomic data, the proteomic data can provide an enhanced understanding of citrus stress/defense responses to HLB.
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factors affecting agrobacterium mediated transformation and regeneration of Sweet Orange and citrange
Plant Cell Tissue and Organ Culture, 2002Co-Authors: Changhe Yu, Chunxian Chen, Zhanao Deng, Paul Ling, Shu Huang, Fred G GmitterjrAbstract:Epicotyl explants of Sweet Orange and citrange were infected with Agrobacterium strain EHA101 harboring binary vector pGA482GG, and factors affecting the plant regeneration and transformation efficiency were evaluated. Increasing the wounded area of explants by cutting longitudinally into two halves, and optimization of inoculation density, dramatically enhanced both regeneration and transformation frequency. Inclusion of 2,4-dichlorophenoxyacetic acid (2,4-D) in the explant pretreatment medium and the co-culture medium improved the transformation efficiency by decreasing the escape frequency. More than 90% rooting frequency of transformed citrange shoots was achieved by two-step culture: first on media supplemented with auxins, and then on media without hormones. Inclusion of 20 mg l−1 kanamycin in rooting medium efficiently discriminated transformed shoots from non-transgenic escaped shoots. Shoot grafting in vitro was used to regenerate transformed plants, due to the slow growth of most Sweet Orange shoots.
Yu Wang - One of the best experts on this subject based on the ideXlab platform.
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differentiation between flavors of Sweet Orange citrus sinensis and mandarin citrus reticulata
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Shi Feng, Frederick G. Gmitter, Joon Hyuk Suh, Yu WangAbstract:Pioneering investigations referring to citrus flavor have been intensively conducted. However, the characteristic flavor difference between Sweet Orange and mandarin has not been defined. In this study, sensory analysis illustrated the crucial role of aroma in the differentiation between Orange flavor and mandarin flavor. To study aroma, Valencia Orange and LB8–9 mandarin were used. Their most aroma-active compounds were preliminarily identified by aroma extract dilution analysis (AEDA). Quantitation of key volatiles followed by calculation of odor activity values (OAVs) further detected potent components (OAV ≥ 1) impacting the overall aromatic profile of Orange/mandarin. Follow-up aroma profile analysis revealed that ethyl butanoate, ethyl 2-methylbutanoate, octanal, decanal, and acetaldehyde were essential for Orange-like aroma, whereas linalool, octanal, α-pinene, limonene, and (E,E)-2,4-decadienal were considered key components for mandarin-like aroma. Furthermore, an unreleased mandarin hybrid produ...
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Differentiation between Flavors of Sweet Orange (Citrus sinensis) and Mandarin (Citrus reticulata)
2017Co-Authors: Shi Feng, Frederick G. Gmitter, Joon Hyuk Suh, Yu WangAbstract:Pioneering investigations referring to citrus flavor have been intensively conducted. However, the characteristic flavor difference between Sweet Orange and mandarin has not been defined. In this study, sensory analysis illustrated the crucial role of aroma in the differentiation between Orange flavor and mandarin flavor. To study aroma, Valencia Orange and LB8–9 mandarin were used. Their most aroma-active compounds were preliminarily identified by aroma extract dilution analysis (AEDA). Quantitation of key volatiles followed by calculation of odor activity values (OAVs) further detected potent components (OAV ≥ 1) impacting the overall aromatic profile of Orange/mandarin. Follow-up aroma profile analysis revealed that ethyl butanoate, ethyl 2-methylbutanoate, octanal, decanal, and acetaldehyde were essential for Orange-like aroma, whereas linalool, octanal, α-pinene, limonene, and (E,E)-2,4-decadienal were considered key components for mandarin-like aroma. Furthermore, an unreleased mandarin hybrid producing fruit with Orange-like flavor was used to validate the identification of characteristic volatiles in Orange-like aroma
Carlos Priminho Pirovani - One of the best experts on this subject based on the ideXlab platform.
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citrus tristeza virus ctv causing proteomic and enzymatic changes in Sweet Orange variety westin
PLOS ONE, 2015Co-Authors: Milena Santos Doria, A O Sousa, Cristiane De Jesus Barbosa, Marcio Gilberto Cardoso Costa, Abelmon Da Silva Gesteira, Regina Martins Souza, Ana Camila Oliveira Freitas, Carlos Priminho PirovaniAbstract:Citrus Tristeza disease, caused by CTV (Citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. The virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of Tristeza. The objective of this study was to analyze the changes caused by CTV in the proteome of stems of Sweet Orange, as well as in the activity and gene expression of antioxidant enzymes. The CTV-infected Sweet Orange displayed mild symptoms, which were characterized by the presence of sparse stem pitting throughout their stems. The presence of virus was confirmed by RT-PCR. Proteomic analysis by 2DE-PAGE-MS / MS revealed the identity of 40 proteins differentially expressed between CTV- infected and -non-infected samples. Of these, 33 were up-regulated and 7 were down-regulated in CTV-infected samples. Among the proteins identified stands out a specific from the virus, the coat protein. Other proteins identified are involved with oxidative stress and for this their enzymatic activity was measured. The activity of superoxide dismutase (SOD) was higher in CTV-infected samples, as catalase (CAT) showed higher activity in uninfected samples. The activity of guaiacol peroxidase (GPX) did not vary significantly between samples. However, ascorbate peroxidase (APX) was more active in the infected samples. The relative expression of the genes encoding CAT, SOD, APX and GPX was analyzed by quantitative real time PCR (RT-qPCR). The CTV-infected samples showed greater accumulation of transcripts, except for the CAT gene. This gene showed higher expression in the uninfected samples. Taken together, it can be concluded that the CTV affects the protein profile and activity and gene expression of antioxidant enzymes in plants infected by this virus.
