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David Kabat - One of the best experts on this subject based on the ideXlab platform.

  • a sodium dependent neutral amino acid transporter mediates infections of feline and baboon Endogenous retroViruses and simian type d retroViruses
    Journal of Virology, 1999
    Co-Authors: Chetankumar S Tailor, Ali Nouri, Yuan Zhao, Yasuhiro Takeuchi, David Kabat
    Abstract:

    The type D simian retroViruses cause immunosuppression in macaques and have been reported as a presumptive opportunistic infection in a patient with AIDS. Previous evidence based on viral interference has strongly suggested that the type D simian Viruses share a common but unknown cell surface receptor with three type C Viruses: feline Endogenous Virus (RD114), baboon Endogenous Virus, and avian reticuloendotheliosis Virus. Furthermore, the receptor gene for these Viruses has been mapped to human chromosome 19q13.1-13.2. We now report the isolation and characterization of a cell surface receptor for this group of retroViruses by using a human T-lymphocyte cDNA library in a retroviral vector. Swiss mouse fibroblasts (NIH 3T3), which are naturally resistant to RD114, were transduced with the retroviral library and then challenged with an RD114-pseudotyped Virus containing a dominant selectable gene for puromycin resistance. Puromycin selection yielded 12 cellular clones that were highly susceptible to a beta-galactosidase-encoding lacZ(RD114) pseudotype Virus. Using PCR primers specific for vector sequences, we amplified a common 2.9-kb product from 10 positive clones. Expression of the 2.9-kb cDNA in Chinese hamster ovary cells conferred susceptibility to RD114, baboon Endogenous Virus, and the type D simian retroViruses. The 2.9-kb cDNA predicted a protein of 541 amino acids that had 98% identity with the previously cloned human Na+-dependent neutral-amino-acid transporter Bo. Accordingly, expression of the RD114 receptor in NIH 3T3 cells resulted in enhanced cellular uptake of L-[3H]alanine and L-[3H]glutamine. RNA blot (Northern) analysis suggested that the RD114 receptor is widely expressed in human tissues and cell lines, including hematopoietic cells. The human Bo transporter gene has been previously mapped to 19q13.3, which is closely linked to the gene locus of the RD114 receptor.

  • a sodium dependent neutral amino acid transporter mediates infections of feline and baboon Endogenous retroViruses and simian type d retroViruses
    Journal of Virology, 1999
    Co-Authors: Chetankumar S Tailor, Ali Nouri, Yuan Zhao, Yasuhiro Takeuchi, David Kabat
    Abstract:

    The type D simian retroViruses cause immunosuppression in macaques and have been reported as a presumptive opportunistic infection in a patient with AIDS. Previous evidence based on viral interference has strongly suggested that the type D simian Viruses share a common but unknown cell surface receptor with three type C Viruses: feline Endogenous Virus (RD114), baboon Endogenous Virus, and avian reticuloendotheliosis Virus. Furthermore, the receptor gene for these Viruses has been mapped to human chromosome 19q13.1–13.2. We now report the isolation and characterization of a cell surface receptor for this group of retroViruses by using a human T-lymphocyte cDNA library in a retroviral vector. Swiss mouse fibroblasts (NIH 3T3), which are naturally resistant to RD114, were transduced with the retroviral library and then challenged with an RD114-pseudotyped Virus containing a dominant selectable gene for puromycin resistance. Puromycin selection yielded 12 cellular clones that were highly susceptible to a β-galactosidase-encoding lacZ(RD114) pseudotype Virus. Using PCR primers specific for vector sequences, we amplified a common 2.9-kb product from 10 positive clones. Expression of the 2.9-kb cDNA in Chinese hamster ovary cells conferred susceptibility to RD114, baboon Endogenous Virus, and the type D simian retroViruses. The 2.9-kb cDNA predicted a protein of 541 amino acids that had 98% identity with the previously cloned human Na + -dependent neutral-amino-acid transporter B o . Accordingly, expression of the RD114 receptor in NIH 3T3 cells resulted in enhanced cellular uptake of l-[ 3 H]alanine andl-[ 3 H]glutamine. RNA blot (Northern) analysis suggested that the RD114 receptor is widely expressed in human tissues and cell lines, including hematopoietic cells. The human B o transporter gene has been previously mapped to 19q13.3, which is closely linked to the gene locus of the RD114 receptor.

Chetankumar S Tailor - One of the best experts on this subject based on the ideXlab platform.

  • a sodium dependent neutral amino acid transporter mediates infections of feline and baboon Endogenous retroViruses and simian type d retroViruses
    Journal of Virology, 1999
    Co-Authors: Chetankumar S Tailor, Ali Nouri, Yuan Zhao, Yasuhiro Takeuchi, David Kabat
    Abstract:

    The type D simian retroViruses cause immunosuppression in macaques and have been reported as a presumptive opportunistic infection in a patient with AIDS. Previous evidence based on viral interference has strongly suggested that the type D simian Viruses share a common but unknown cell surface receptor with three type C Viruses: feline Endogenous Virus (RD114), baboon Endogenous Virus, and avian reticuloendotheliosis Virus. Furthermore, the receptor gene for these Viruses has been mapped to human chromosome 19q13.1-13.2. We now report the isolation and characterization of a cell surface receptor for this group of retroViruses by using a human T-lymphocyte cDNA library in a retroviral vector. Swiss mouse fibroblasts (NIH 3T3), which are naturally resistant to RD114, were transduced with the retroviral library and then challenged with an RD114-pseudotyped Virus containing a dominant selectable gene for puromycin resistance. Puromycin selection yielded 12 cellular clones that were highly susceptible to a beta-galactosidase-encoding lacZ(RD114) pseudotype Virus. Using PCR primers specific for vector sequences, we amplified a common 2.9-kb product from 10 positive clones. Expression of the 2.9-kb cDNA in Chinese hamster ovary cells conferred susceptibility to RD114, baboon Endogenous Virus, and the type D simian retroViruses. The 2.9-kb cDNA predicted a protein of 541 amino acids that had 98% identity with the previously cloned human Na+-dependent neutral-amino-acid transporter Bo. Accordingly, expression of the RD114 receptor in NIH 3T3 cells resulted in enhanced cellular uptake of L-[3H]alanine and L-[3H]glutamine. RNA blot (Northern) analysis suggested that the RD114 receptor is widely expressed in human tissues and cell lines, including hematopoietic cells. The human Bo transporter gene has been previously mapped to 19q13.3, which is closely linked to the gene locus of the RD114 receptor.

  • a sodium dependent neutral amino acid transporter mediates infections of feline and baboon Endogenous retroViruses and simian type d retroViruses
    Journal of Virology, 1999
    Co-Authors: Chetankumar S Tailor, Ali Nouri, Yuan Zhao, Yasuhiro Takeuchi, David Kabat
    Abstract:

    The type D simian retroViruses cause immunosuppression in macaques and have been reported as a presumptive opportunistic infection in a patient with AIDS. Previous evidence based on viral interference has strongly suggested that the type D simian Viruses share a common but unknown cell surface receptor with three type C Viruses: feline Endogenous Virus (RD114), baboon Endogenous Virus, and avian reticuloendotheliosis Virus. Furthermore, the receptor gene for these Viruses has been mapped to human chromosome 19q13.1–13.2. We now report the isolation and characterization of a cell surface receptor for this group of retroViruses by using a human T-lymphocyte cDNA library in a retroviral vector. Swiss mouse fibroblasts (NIH 3T3), which are naturally resistant to RD114, were transduced with the retroviral library and then challenged with an RD114-pseudotyped Virus containing a dominant selectable gene for puromycin resistance. Puromycin selection yielded 12 cellular clones that were highly susceptible to a β-galactosidase-encoding lacZ(RD114) pseudotype Virus. Using PCR primers specific for vector sequences, we amplified a common 2.9-kb product from 10 positive clones. Expression of the 2.9-kb cDNA in Chinese hamster ovary cells conferred susceptibility to RD114, baboon Endogenous Virus, and the type D simian retroViruses. The 2.9-kb cDNA predicted a protein of 541 amino acids that had 98% identity with the previously cloned human Na + -dependent neutral-amino-acid transporter B o . Accordingly, expression of the RD114 receptor in NIH 3T3 cells resulted in enhanced cellular uptake of l-[ 3 H]alanine andl-[ 3 H]glutamine. RNA blot (Northern) analysis suggested that the RD114 receptor is widely expressed in human tissues and cell lines, including hematopoietic cells. The human B o transporter gene has been previously mapped to 19q13.3, which is closely linked to the gene locus of the RD114 receptor.

Yasuhiro Takeuchi - One of the best experts on this subject based on the ideXlab platform.

  • a sodium dependent neutral amino acid transporter mediates infections of feline and baboon Endogenous retroViruses and simian type d retroViruses
    Journal of Virology, 1999
    Co-Authors: Chetankumar S Tailor, Ali Nouri, Yuan Zhao, Yasuhiro Takeuchi, David Kabat
    Abstract:

    The type D simian retroViruses cause immunosuppression in macaques and have been reported as a presumptive opportunistic infection in a patient with AIDS. Previous evidence based on viral interference has strongly suggested that the type D simian Viruses share a common but unknown cell surface receptor with three type C Viruses: feline Endogenous Virus (RD114), baboon Endogenous Virus, and avian reticuloendotheliosis Virus. Furthermore, the receptor gene for these Viruses has been mapped to human chromosome 19q13.1-13.2. We now report the isolation and characterization of a cell surface receptor for this group of retroViruses by using a human T-lymphocyte cDNA library in a retroviral vector. Swiss mouse fibroblasts (NIH 3T3), which are naturally resistant to RD114, were transduced with the retroviral library and then challenged with an RD114-pseudotyped Virus containing a dominant selectable gene for puromycin resistance. Puromycin selection yielded 12 cellular clones that were highly susceptible to a beta-galactosidase-encoding lacZ(RD114) pseudotype Virus. Using PCR primers specific for vector sequences, we amplified a common 2.9-kb product from 10 positive clones. Expression of the 2.9-kb cDNA in Chinese hamster ovary cells conferred susceptibility to RD114, baboon Endogenous Virus, and the type D simian retroViruses. The 2.9-kb cDNA predicted a protein of 541 amino acids that had 98% identity with the previously cloned human Na+-dependent neutral-amino-acid transporter Bo. Accordingly, expression of the RD114 receptor in NIH 3T3 cells resulted in enhanced cellular uptake of L-[3H]alanine and L-[3H]glutamine. RNA blot (Northern) analysis suggested that the RD114 receptor is widely expressed in human tissues and cell lines, including hematopoietic cells. The human Bo transporter gene has been previously mapped to 19q13.3, which is closely linked to the gene locus of the RD114 receptor.

  • a sodium dependent neutral amino acid transporter mediates infections of feline and baboon Endogenous retroViruses and simian type d retroViruses
    Journal of Virology, 1999
    Co-Authors: Chetankumar S Tailor, Ali Nouri, Yuan Zhao, Yasuhiro Takeuchi, David Kabat
    Abstract:

    The type D simian retroViruses cause immunosuppression in macaques and have been reported as a presumptive opportunistic infection in a patient with AIDS. Previous evidence based on viral interference has strongly suggested that the type D simian Viruses share a common but unknown cell surface receptor with three type C Viruses: feline Endogenous Virus (RD114), baboon Endogenous Virus, and avian reticuloendotheliosis Virus. Furthermore, the receptor gene for these Viruses has been mapped to human chromosome 19q13.1–13.2. We now report the isolation and characterization of a cell surface receptor for this group of retroViruses by using a human T-lymphocyte cDNA library in a retroviral vector. Swiss mouse fibroblasts (NIH 3T3), which are naturally resistant to RD114, were transduced with the retroviral library and then challenged with an RD114-pseudotyped Virus containing a dominant selectable gene for puromycin resistance. Puromycin selection yielded 12 cellular clones that were highly susceptible to a β-galactosidase-encoding lacZ(RD114) pseudotype Virus. Using PCR primers specific for vector sequences, we amplified a common 2.9-kb product from 10 positive clones. Expression of the 2.9-kb cDNA in Chinese hamster ovary cells conferred susceptibility to RD114, baboon Endogenous Virus, and the type D simian retroViruses. The 2.9-kb cDNA predicted a protein of 541 amino acids that had 98% identity with the previously cloned human Na + -dependent neutral-amino-acid transporter B o . Accordingly, expression of the RD114 receptor in NIH 3T3 cells resulted in enhanced cellular uptake of l-[ 3 H]alanine andl-[ 3 H]glutamine. RNA blot (Northern) analysis suggested that the RD114 receptor is widely expressed in human tissues and cell lines, including hematopoietic cells. The human B o transporter gene has been previously mapped to 19q13.3, which is closely linked to the gene locus of the RD114 receptor.

  • high titer packaging cells producing recombinant retroViruses resistant to human serum
    Journal of Virology, 1995
    Co-Authors: Francoisloic Cosset, Yasuhiro Takeuchi, J L Battini, Robin A Weiss, Mary Collins
    Abstract:

    Novel retroviral protein expression constructs were designed to retain minimal retroviral sequences and to express dominant selectable markers by reinitiation of translation after expression of the viral genes. HT1080 cells were selected as producer cells for their ability to release high-titer Viruses that are resistant to inactivation by human serum. Two HT1080-based packaging cell lines which produce Moloney murine leukemia Virus cores with envelope glycoproteins of either amphotropic murine leukemia Virus (FLYA13 line) or cat Endogenous Virus RD114 (FLYRD18 line) are described. Direct comparison with previous retroviral packaging systems indicated that 100-fold-higher titers of helper-free recombinant Viruses were released by the FLYA13 and FLYRD18 lines.

Jaap Goudsmit - One of the best experts on this subject based on the ideXlab platform.

  • Complete nucleotide sequence of simian Endogenous type D retroVirus with intact genome organization: evidence for ancestry to simian retroVirus and baboon Endogenous Virus
    Journal of virology, 1997
    Co-Authors: A C Van Der Kuyl, John T. Dekker, Rui Mang, Jaap Goudsmit
    Abstract:

    A complete Endogenous type D viral genome has been isolated from a baboon genomic library. The proVirus, simian Endogenous retroVirus (SERV), is 8,393 nucleotides long and contains two long terminal repeats and complete genes for gag, pro, pol, and env. The primer binding site is complementary to tRNA(Lys)3, like in lentiViruses. The env GP70 protein is highly homologous to that of baboon Endogenous Virus (BaEV). PCR analysis of primate DNA showed that related proviral sequences are present in Old World monkeys of the subfamily Cercopithecinae but not in apes and humans. Analysis of Virus and host sequences indicated that the proviral genomes were inherited from a common ancestor. Comparison of the evolution of BaEV, exogenous simian retroVirus types 1 to 3 (SRV1 to SRV3), and SERV suggests that SERV is ancestral to both BaEV and the SRVs.

  • for Ancestry to Simian RetroVirus and Baboon Endogenous Virus
    1996
    Co-Authors: J. Virol, A C Van Der Kuyl, John T. Dekker, Jaap Goudsmit, Rui Mang, J T Dekker
    Abstract:

    Virus. simian retroVirus and baboon Endogenous genome organization: evidence for ancestry to Endogenous type D retroVirus with intact Complete nucleotide sequence of simia

  • Full-length proViruses of baboon Endogenous Virus (BaEV) and dispersed BaEV reverse transcriptase retroelements in the genome of baboon species.
    Journal of virology, 1995
    Co-Authors: A C Van Der Kuyl, John T. Dekker, Jaap Goudsmit
    Abstract:

    Baboon Endogenous Virus (BaEV) is present in multiple copies in many Old World monkey species. BaEV proViruses may contain open reading frames for all major genes, as is indicated by the rescue of infectious Virus particles from baboon and gelada tissues after cocultivation with permissive cells. We have analyzed full-length BaEV proviral structures in a baboon (Papio cynocephalus) genomic library and found no evidence for the rearrangements or large deletions commonly observed in Endogenous Virus genomes from other mammalian species. The two proViruses studied were integrated next to or nearby long interspersed repeat sequence (LINE) transposable elements. Additionally, isolated dispersed fragments with 100% and approximately 77% homology, respectively, to part of the BaEV reverse transcriptase gene were detected. These presumed retroelements were present in an approximately 10-fold excess compared with the full-length proviral genomes. PCR amplification and sequencing of BaEV reverse transcriptase and env fragments from the lambda clones and from the genomic DNA of other baboon species showed that there is little sequence variation present in BaEV DNA in the baboon genome.

Dusty A Miller - One of the best experts on this subject based on the ideXlab platform.

  • the rd114 simian type d retroVirus receptor is a neutral amino acid transporter
    Proceedings of the National Academy of Sciences of the United States of America, 1999
    Co-Authors: John E. J. Rasko, Jeanluc Battini, Rebecca J Gottschalk, Ilya Mazo, Dusty A Miller
    Abstract:

    The RD114/simian type D retroViruses, which include the feline Endogenous retroVirus RD114, all strains of simian immunosuppressive type D retroViruses, the avian reticuloendotheliosis group including spleen necrosis Virus, and baboon Endogenous Virus, use a common cell-surface receptor for cell entry. We have used a retroviral cDNA library approach, involving transfer and expression of cDNAs from highly infectable HeLa cells to nonpermissive NIH 3T3 mouse cells, to clone and identify this receptor. The cloned cDNA, denoted RDR, is an allele of the previously cloned neutral amino acid transporter ATB0 (SLC1A5). Both RDR and ATB0 serve as retroVirus receptors and both show specific transport of neutral amino acids. We have localized the receptor by radiation hybrid mapping to a region of about 500-kb pairs on the long arm of human chromosome 19 at q13.3. Infection of cells with RD114/type D retroViruses results in impaired amino acid transport, suggesting a mechanism for Virus toxicity and immunosuppression. The identification and functional characterization of this retroVirus receptor provide insight into the retroVirus life cycle and pathogenesis and will be an important tool for optimization of gene therapy using vectors derived from RD114/type D retroViruses.