The Experts below are selected from a list of 456738 Experts worldwide ranked by ideXlab platform
Minoru Ueda - One of the best experts on this subject based on the ideXlab platform.
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a feasibility of useful cell based therapy by bone regeneration with deciduous tooth stem cells dental pulp stem cells or bone marrow derived mesenchymal stem cells for clinical study using tissue Engineering Technology
Tissue Engineering Part A, 2010Co-Authors: Yoichi Yamada, Sayaka Nakamura, Takayuki Sugito, Ryoko Yoshimi, Tetsuro Nagasaka, Minoru UedaAbstract:This study investigated the effect of bone regeneration with dental pulp stem cells (DPSCs), deciduous tooth stem cells (DTSCs), or bone-marrow-derived mesenchymal stem cells (BMMSCs) for clinical study on hydroxyapatite-coated osseointegrated dental implants, using tissue Engineering Technology. In vitro, human DPSCs and DTSCs expressed STRO-1, CD13, CD29, CD 44, CD73, and osteogenic marker genes such as alkaline phosphatase, Runx2, and osteocalcin. In vivo, prepared bone defect model was implanted using graft materials as follows: platelet-rich plasma (PRP), PRP and canine BMMSCs (cBMMSCs), PRP and canine DPSCs (cDPSCs), PRP and puppy DTSCs (pDTSCs), and control (defect only). After 8 weeks, the dental implants were installed, and 16 weeks later the sections were evaluated histologically and histometrically. The cBMMSCs/PRP, cDPSCs/PRP, and pDTSCs/PRP groups had well-formed mature bone and neovascularization. Histometrically, the bone–implant contact was significantly different between the cBMMSCs/PRP, ...
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a feasibility of useful cell based therapy by bone regeneration with deciduous tooth stem cells dental pulp stem cells or bone marrow derived mesenchymal stem cells for clinical study using tissue Engineering Technology
Tissue Engineering Part A, 2010Co-Authors: Yoichi M A Yamada, Sayaka Nakamura, Takayuki Sugito, Ryoko Yoshimi, Tetsuro Nagasaka, Kenji Ito, Minoru UedaAbstract:This study investigated the effect of bone regeneration with dental pulp stem cells (DPSCs), deciduous tooth stem cells (DTSCs), or bone-marrow-derived mesenchymal stem cells (BMMSCs) for clinical study on hydroxyapatite-coated osseointegrated dental implants, using tissue Engineering Technology. In vitro, human DPSCs and DTSCs expressed STRO-1, CD13, CD29, CD 44, CD73, and osteogenic marker genes such as alkaline phosphatase, Runx2, and osteocalcin. In vivo, prepared bone defect model was implanted using graft materials as follows: platelet-rich plasma (PRP), PRP and canine BMMSCs (cBMMSCs), PRP and canine DPSCs (cDPSCs), PRP and puppy DTSCs (pDTSCs), and control (defect only). After 8 weeks, the dental implants were installed, and 16 weeks later the sections were evaluated histologically and histometrically. The cBMMSCs/PRP, cDPSCs/PRP, and pDTSCs/PRP groups had well-formed mature bone and neovascularization. Histometrically, the bone-implant contact was significantly different between the cBMMSCs/PRP, cDPSCs/PRP, pDTSCs/PRP groups, and the control and PRP groups (p < 0.01). These results demonstrated that these stem cells with PRP have the ability to form bone, and this bone formation activity might be useful for osseointegrated hydroxyapatite-coated dental implants with good levels of bone-implant contact.
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a novel approach to periodontal tissue regeneration with mesenchymal stem cells and platelet rich plasma using tissue Engineering Technology a clinical case report
International Journal of Periodontics & Restorative Dentistry, 2006Co-Authors: Yoichi Yamada, Minoru Ueda, Hideharu Hibi, Shunsuke BabaAbstract:: Tissue Engineering represents one of the most exciting advances in regenerative medicine. However, little has been reported on the application of tissue Engineering for regeneration of periodontal tissues. Therefore, the aim of this study was to show how a technique based on tissue Engineering principles can be applied to periodontology. Mesenchymal stem cells (MSCs) were isolated from a patient's iliac crest marrow aspirates. Platelet-rich plasma (PRP) was isolated from peripheral blood. Full-thickness periodontal flaps were elevated and the root surfaces were scaled and planed. A MSCs-PRP gel was prepared and applied to the root surface and adjacent defect space. The primary outcome measures were changes in pocket depth, clinical attachment level, bleeding on probing, and defect bone fill. Re-examination demonstrated that the treatment, including the application of MSCs-PRP gel at periodontal sites with angular defects, resulted in a 4-mm reduction in probing depths and a 4-mm clinical attachment gain, while bleeding and tooth mobility disappeared. Radiographic assessments showed that the bone defect had been reduced in depth. Interdental papillae supported by this tissue Engineering Technology regenerated. The use of MSCs in PRP gel might be helpful for periodontal tissue regeneration, treatment of esthetically sensitive sites, and reduction of patient morbidity.
Ryoko Yoshimi - One of the best experts on this subject based on the ideXlab platform.
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a feasibility of useful cell based therapy by bone regeneration with deciduous tooth stem cells dental pulp stem cells or bone marrow derived mesenchymal stem cells for clinical study using tissue Engineering Technology
Tissue Engineering Part A, 2010Co-Authors: Yoichi Yamada, Sayaka Nakamura, Takayuki Sugito, Ryoko Yoshimi, Tetsuro Nagasaka, Minoru UedaAbstract:This study investigated the effect of bone regeneration with dental pulp stem cells (DPSCs), deciduous tooth stem cells (DTSCs), or bone-marrow-derived mesenchymal stem cells (BMMSCs) for clinical study on hydroxyapatite-coated osseointegrated dental implants, using tissue Engineering Technology. In vitro, human DPSCs and DTSCs expressed STRO-1, CD13, CD29, CD 44, CD73, and osteogenic marker genes such as alkaline phosphatase, Runx2, and osteocalcin. In vivo, prepared bone defect model was implanted using graft materials as follows: platelet-rich plasma (PRP), PRP and canine BMMSCs (cBMMSCs), PRP and canine DPSCs (cDPSCs), PRP and puppy DTSCs (pDTSCs), and control (defect only). After 8 weeks, the dental implants were installed, and 16 weeks later the sections were evaluated histologically and histometrically. The cBMMSCs/PRP, cDPSCs/PRP, and pDTSCs/PRP groups had well-formed mature bone and neovascularization. Histometrically, the bone–implant contact was significantly different between the cBMMSCs/PRP, ...
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a feasibility of useful cell based therapy by bone regeneration with deciduous tooth stem cells dental pulp stem cells or bone marrow derived mesenchymal stem cells for clinical study using tissue Engineering Technology
Tissue Engineering Part A, 2010Co-Authors: Yoichi M A Yamada, Sayaka Nakamura, Takayuki Sugito, Ryoko Yoshimi, Tetsuro Nagasaka, Kenji Ito, Minoru UedaAbstract:This study investigated the effect of bone regeneration with dental pulp stem cells (DPSCs), deciduous tooth stem cells (DTSCs), or bone-marrow-derived mesenchymal stem cells (BMMSCs) for clinical study on hydroxyapatite-coated osseointegrated dental implants, using tissue Engineering Technology. In vitro, human DPSCs and DTSCs expressed STRO-1, CD13, CD29, CD 44, CD73, and osteogenic marker genes such as alkaline phosphatase, Runx2, and osteocalcin. In vivo, prepared bone defect model was implanted using graft materials as follows: platelet-rich plasma (PRP), PRP and canine BMMSCs (cBMMSCs), PRP and canine DPSCs (cDPSCs), PRP and puppy DTSCs (pDTSCs), and control (defect only). After 8 weeks, the dental implants were installed, and 16 weeks later the sections were evaluated histologically and histometrically. The cBMMSCs/PRP, cDPSCs/PRP, and pDTSCs/PRP groups had well-formed mature bone and neovascularization. Histometrically, the bone-implant contact was significantly different between the cBMMSCs/PRP, cDPSCs/PRP, pDTSCs/PRP groups, and the control and PRP groups (p < 0.01). These results demonstrated that these stem cells with PRP have the ability to form bone, and this bone formation activity might be useful for osseointegrated hydroxyapatite-coated dental implants with good levels of bone-implant contact.
Sayaka Nakamura - One of the best experts on this subject based on the ideXlab platform.
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a feasibility of useful cell based therapy by bone regeneration with deciduous tooth stem cells dental pulp stem cells or bone marrow derived mesenchymal stem cells for clinical study using tissue Engineering Technology
Tissue Engineering Part A, 2010Co-Authors: Yoichi Yamada, Sayaka Nakamura, Takayuki Sugito, Ryoko Yoshimi, Tetsuro Nagasaka, Minoru UedaAbstract:This study investigated the effect of bone regeneration with dental pulp stem cells (DPSCs), deciduous tooth stem cells (DTSCs), or bone-marrow-derived mesenchymal stem cells (BMMSCs) for clinical study on hydroxyapatite-coated osseointegrated dental implants, using tissue Engineering Technology. In vitro, human DPSCs and DTSCs expressed STRO-1, CD13, CD29, CD 44, CD73, and osteogenic marker genes such as alkaline phosphatase, Runx2, and osteocalcin. In vivo, prepared bone defect model was implanted using graft materials as follows: platelet-rich plasma (PRP), PRP and canine BMMSCs (cBMMSCs), PRP and canine DPSCs (cDPSCs), PRP and puppy DTSCs (pDTSCs), and control (defect only). After 8 weeks, the dental implants were installed, and 16 weeks later the sections were evaluated histologically and histometrically. The cBMMSCs/PRP, cDPSCs/PRP, and pDTSCs/PRP groups had well-formed mature bone and neovascularization. Histometrically, the bone–implant contact was significantly different between the cBMMSCs/PRP, ...
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a feasibility of useful cell based therapy by bone regeneration with deciduous tooth stem cells dental pulp stem cells or bone marrow derived mesenchymal stem cells for clinical study using tissue Engineering Technology
Tissue Engineering Part A, 2010Co-Authors: Yoichi M A Yamada, Sayaka Nakamura, Takayuki Sugito, Ryoko Yoshimi, Tetsuro Nagasaka, Kenji Ito, Minoru UedaAbstract:This study investigated the effect of bone regeneration with dental pulp stem cells (DPSCs), deciduous tooth stem cells (DTSCs), or bone-marrow-derived mesenchymal stem cells (BMMSCs) for clinical study on hydroxyapatite-coated osseointegrated dental implants, using tissue Engineering Technology. In vitro, human DPSCs and DTSCs expressed STRO-1, CD13, CD29, CD 44, CD73, and osteogenic marker genes such as alkaline phosphatase, Runx2, and osteocalcin. In vivo, prepared bone defect model was implanted using graft materials as follows: platelet-rich plasma (PRP), PRP and canine BMMSCs (cBMMSCs), PRP and canine DPSCs (cDPSCs), PRP and puppy DTSCs (pDTSCs), and control (defect only). After 8 weeks, the dental implants were installed, and 16 weeks later the sections were evaluated histologically and histometrically. The cBMMSCs/PRP, cDPSCs/PRP, and pDTSCs/PRP groups had well-formed mature bone and neovascularization. Histometrically, the bone-implant contact was significantly different between the cBMMSCs/PRP, cDPSCs/PRP, pDTSCs/PRP groups, and the control and PRP groups (p < 0.01). These results demonstrated that these stem cells with PRP have the ability to form bone, and this bone formation activity might be useful for osseointegrated hydroxyapatite-coated dental implants with good levels of bone-implant contact.
Takayuki Sugito - One of the best experts on this subject based on the ideXlab platform.
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a feasibility of useful cell based therapy by bone regeneration with deciduous tooth stem cells dental pulp stem cells or bone marrow derived mesenchymal stem cells for clinical study using tissue Engineering Technology
Tissue Engineering Part A, 2010Co-Authors: Yoichi Yamada, Sayaka Nakamura, Takayuki Sugito, Ryoko Yoshimi, Tetsuro Nagasaka, Minoru UedaAbstract:This study investigated the effect of bone regeneration with dental pulp stem cells (DPSCs), deciduous tooth stem cells (DTSCs), or bone-marrow-derived mesenchymal stem cells (BMMSCs) for clinical study on hydroxyapatite-coated osseointegrated dental implants, using tissue Engineering Technology. In vitro, human DPSCs and DTSCs expressed STRO-1, CD13, CD29, CD 44, CD73, and osteogenic marker genes such as alkaline phosphatase, Runx2, and osteocalcin. In vivo, prepared bone defect model was implanted using graft materials as follows: platelet-rich plasma (PRP), PRP and canine BMMSCs (cBMMSCs), PRP and canine DPSCs (cDPSCs), PRP and puppy DTSCs (pDTSCs), and control (defect only). After 8 weeks, the dental implants were installed, and 16 weeks later the sections were evaluated histologically and histometrically. The cBMMSCs/PRP, cDPSCs/PRP, and pDTSCs/PRP groups had well-formed mature bone and neovascularization. Histometrically, the bone–implant contact was significantly different between the cBMMSCs/PRP, ...
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a feasibility of useful cell based therapy by bone regeneration with deciduous tooth stem cells dental pulp stem cells or bone marrow derived mesenchymal stem cells for clinical study using tissue Engineering Technology
Tissue Engineering Part A, 2010Co-Authors: Yoichi M A Yamada, Sayaka Nakamura, Takayuki Sugito, Ryoko Yoshimi, Tetsuro Nagasaka, Kenji Ito, Minoru UedaAbstract:This study investigated the effect of bone regeneration with dental pulp stem cells (DPSCs), deciduous tooth stem cells (DTSCs), or bone-marrow-derived mesenchymal stem cells (BMMSCs) for clinical study on hydroxyapatite-coated osseointegrated dental implants, using tissue Engineering Technology. In vitro, human DPSCs and DTSCs expressed STRO-1, CD13, CD29, CD 44, CD73, and osteogenic marker genes such as alkaline phosphatase, Runx2, and osteocalcin. In vivo, prepared bone defect model was implanted using graft materials as follows: platelet-rich plasma (PRP), PRP and canine BMMSCs (cBMMSCs), PRP and canine DPSCs (cDPSCs), PRP and puppy DTSCs (pDTSCs), and control (defect only). After 8 weeks, the dental implants were installed, and 16 weeks later the sections were evaluated histologically and histometrically. The cBMMSCs/PRP, cDPSCs/PRP, and pDTSCs/PRP groups had well-formed mature bone and neovascularization. Histometrically, the bone-implant contact was significantly different between the cBMMSCs/PRP, cDPSCs/PRP, pDTSCs/PRP groups, and the control and PRP groups (p < 0.01). These results demonstrated that these stem cells with PRP have the ability to form bone, and this bone formation activity might be useful for osseointegrated hydroxyapatite-coated dental implants with good levels of bone-implant contact.
Tetsuro Nagasaka - One of the best experts on this subject based on the ideXlab platform.
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a feasibility of useful cell based therapy by bone regeneration with deciduous tooth stem cells dental pulp stem cells or bone marrow derived mesenchymal stem cells for clinical study using tissue Engineering Technology
Tissue Engineering Part A, 2010Co-Authors: Yoichi Yamada, Sayaka Nakamura, Takayuki Sugito, Ryoko Yoshimi, Tetsuro Nagasaka, Minoru UedaAbstract:This study investigated the effect of bone regeneration with dental pulp stem cells (DPSCs), deciduous tooth stem cells (DTSCs), or bone-marrow-derived mesenchymal stem cells (BMMSCs) for clinical study on hydroxyapatite-coated osseointegrated dental implants, using tissue Engineering Technology. In vitro, human DPSCs and DTSCs expressed STRO-1, CD13, CD29, CD 44, CD73, and osteogenic marker genes such as alkaline phosphatase, Runx2, and osteocalcin. In vivo, prepared bone defect model was implanted using graft materials as follows: platelet-rich plasma (PRP), PRP and canine BMMSCs (cBMMSCs), PRP and canine DPSCs (cDPSCs), PRP and puppy DTSCs (pDTSCs), and control (defect only). After 8 weeks, the dental implants were installed, and 16 weeks later the sections were evaluated histologically and histometrically. The cBMMSCs/PRP, cDPSCs/PRP, and pDTSCs/PRP groups had well-formed mature bone and neovascularization. Histometrically, the bone–implant contact was significantly different between the cBMMSCs/PRP, ...
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a feasibility of useful cell based therapy by bone regeneration with deciduous tooth stem cells dental pulp stem cells or bone marrow derived mesenchymal stem cells for clinical study using tissue Engineering Technology
Tissue Engineering Part A, 2010Co-Authors: Yoichi M A Yamada, Sayaka Nakamura, Takayuki Sugito, Ryoko Yoshimi, Tetsuro Nagasaka, Kenji Ito, Minoru UedaAbstract:This study investigated the effect of bone regeneration with dental pulp stem cells (DPSCs), deciduous tooth stem cells (DTSCs), or bone-marrow-derived mesenchymal stem cells (BMMSCs) for clinical study on hydroxyapatite-coated osseointegrated dental implants, using tissue Engineering Technology. In vitro, human DPSCs and DTSCs expressed STRO-1, CD13, CD29, CD 44, CD73, and osteogenic marker genes such as alkaline phosphatase, Runx2, and osteocalcin. In vivo, prepared bone defect model was implanted using graft materials as follows: platelet-rich plasma (PRP), PRP and canine BMMSCs (cBMMSCs), PRP and canine DPSCs (cDPSCs), PRP and puppy DTSCs (pDTSCs), and control (defect only). After 8 weeks, the dental implants were installed, and 16 weeks later the sections were evaluated histologically and histometrically. The cBMMSCs/PRP, cDPSCs/PRP, and pDTSCs/PRP groups had well-formed mature bone and neovascularization. Histometrically, the bone-implant contact was significantly different between the cBMMSCs/PRP, cDPSCs/PRP, pDTSCs/PRP groups, and the control and PRP groups (p < 0.01). These results demonstrated that these stem cells with PRP have the ability to form bone, and this bone formation activity might be useful for osseointegrated hydroxyapatite-coated dental implants with good levels of bone-implant contact.