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Tianwei Tan - One of the best experts on this subject based on the ideXlab platform.

  • The effects of four different pretreatments on Enzymatic Hydrolysis of sweet sorghum bagasse.
    Bioresource technology, 2010
    Co-Authors: Jingzhi Zhang, Xu Zhang, Tianwei Tan
    Abstract:

    Four pretreatment processes including ionic liquids, steam explosion, lime, and dilute acid were used for Enzymatic Hydrolysis of sweet sorghum bagasse. Compared with the other three pretreatment approaches, steam-explosion pretreatment showed the greatest improvement on Enzymatic Hydrolysis of the bagasse. The maximum conversion of cellulose and the concentration of glucose obtained from Enzymatic Hydrolysis of steam explosion bagasse reached 70% and 25 g/L, respectively, which were both 2.5 times higher than those of the control (27% and 11 g/L). The results based on the analysis of SEM photos, FTIR, XRD and NMR detection suggested that both the reduction of crystallite size of cellulose and cellulose degradation from the Iα and Iβ to the Fibril surface cellulose and amorphous cellulose were critical for Enzymatic Hydrolysis. These pretreatments disrupted the crystal structure of cellulose and increased the available surface area, which made the cellulose better accessible for Enzymatic Hydrolysis.

Hongzhang Chen - One of the best experts on this subject based on the ideXlab platform.

  • Correlation of porous structure, mass transfer and Enzymatic Hydrolysis of steam exploded corn stover
    Chemical Engineering Science, 2013
    Co-Authors: Junying Zhao, Hongzhang Chen
    Abstract:

    Establishing the relationships between intrinsic structure and Enzymatic Hydrolysis of plant biomass is critical for understanding the process of Enzymatic Hydrolysis. This manuscript aims to explore the relationships between porous structure and Enzymatic Hydrolysis by examining the pore size distribution and the parameters of mass transfer in steam exploded corn stover. Results indicated that the pore size distribution and other parameters of porous structure of corn stover were altered by steam explosion pretreatment. These structural changes enhanced the percolation probability and permeability, reduced the threshold pressure, and subsequently improved the Enzymatic Hydrolysis yield of steam exploded corn stover. Multiple factors regression analysis demonstrated that threshold pressure was a significant factor of Enzymatic Hydrolysis with a correlation coefficient of 0.885. It revealed that the changes of porous structure could reduce threshold pressure and subsequently improve Enzymatic Hydrolysis. Based on these results, we have proposed the concept of seepage recalcitrance which provides a key index for pretreatment technology. (C) 2013 Elsevier Ltd. All rights reserved.

  • Enhanced Enzymatic Hydrolysis of wheat straw by aqueous glycerol pretreatment.
    Bioresource technology, 2008
    Co-Authors: Fubao Sun, Hongzhang Chen
    Abstract:

    Abstract Considering the practical technology–economy of glycerol processing from oleochemicals industry, the ensuing work was proposed to further explore the atmospheric aqueous glycerol autocatalytic organosolv pretreatment (AAGAOP) to improve the Enzymatic Hydrolysis of lignocellulosic biomass. With the liquid–solid ratio of 20 g g −1 at 220 °C for 3 h, the AAGAOP enabled wheat straw to remove ∼70% hemicelluloses and ∼65% lignin, with ∼98% cellulose retention. The pretreated fiber was achieved with ∼90% of the Enzymatic Hydrolysis yield after 48 h. At oven-drying, dehydration was likely to cause the hornification of fiber, which was responsible for the low Enzymatic Hydrolysis of dried fiber. With SEM observations, the AAGAOP disrupted wheat straw into thin and fine fibrils, with a small average size and more surface area. The AAGAOP technique, as a novel strategy, enhanced the Enzymatic Hydrolysis of lignocellulosic biomass by removing the chemically compositional barrier and altering the physically structural impediment.

Jingzhi Zhang - One of the best experts on this subject based on the ideXlab platform.

  • The effects of four different pretreatments on Enzymatic Hydrolysis of sweet sorghum bagasse.
    Bioresource technology, 2010
    Co-Authors: Jingzhi Zhang, Xu Zhang, Tianwei Tan
    Abstract:

    Four pretreatment processes including ionic liquids, steam explosion, lime, and dilute acid were used for Enzymatic Hydrolysis of sweet sorghum bagasse. Compared with the other three pretreatment approaches, steam-explosion pretreatment showed the greatest improvement on Enzymatic Hydrolysis of the bagasse. The maximum conversion of cellulose and the concentration of glucose obtained from Enzymatic Hydrolysis of steam explosion bagasse reached 70% and 25 g/L, respectively, which were both 2.5 times higher than those of the control (27% and 11 g/L). The results based on the analysis of SEM photos, FTIR, XRD and NMR detection suggested that both the reduction of crystallite size of cellulose and cellulose degradation from the Iα and Iβ to the Fibril surface cellulose and amorphous cellulose were critical for Enzymatic Hydrolysis. These pretreatments disrupted the crystal structure of cellulose and increased the available surface area, which made the cellulose better accessible for Enzymatic Hydrolysis.

Guangtao Cong - One of the best experts on this subject based on the ideXlab platform.

  • Impact of Lignin Content on the Sweet Sorghum Bagasse Enzymatic Hydrolysis
    Energy Procedia, 2014
    Co-Authors: Zhipei Yan, Ting Cui, Yan Jiang, Guangtao Cong
    Abstract:

    Abstract Lignin has been considered as an important factor to effect the Enzymatic Hydrolysis of lignocellulose. In this study, impact of lignin content on Enzymatic Hydrolysis was investigated using sweet sorghum bagasse (SSB) delignified by NaOH or Ca(OH) 2 pretreatment. For NaOH pretreated samples, a negative correlation between cellulose conversion rate and the residual lignin content has been found when the lignin content is between 18.93% and 8.72%. Further delignification of sample can’t increase efficiency of Enzymatic Hydrolysis anymore. For Ca(OH) 2 pretreatment, there is no correlation between lignin content and cellulose conversion rate. More interesting is that the lignin content of SSB pretreated with 10% NaOH (17.32%) is closed to that pretreated with 10% Ca(OH) 2 (16.42%), but the cellulose conversion rate of the latter was 1.71 times of the former. While the surface lignin content of 10% NaOH pretreated SSB was 1.52 times higher than Ca(OH) 2 pretreated SSB. These results demonstrated that the impact of lignin content on Enzymatic Hydrolysis of NaOH and Ca(OH) 2 pretreated SSB was different. The lignin removal is mainly contributed to the Enzymatic Hydrolysis of SSB treated with NaOH, while the contribution of Ca(OH) 2 to the Enzymatic Hydrolysis of SSB was complex and needed to be studied further.

Guido Zacchi - One of the best experts on this subject based on the ideXlab platform.

  • Combined steam pretreatment and Enzymatic Hydrolysis of starch-free wheat fibers.
    Applied biochemistry and biotechnology, 2004
    Co-Authors: Beatriz Palmarola-adrados, Mats Galbe, Guido Zacchi
    Abstract:

    Steam treatment of an industrial process stream, denoted starch-free wheat fiber, was investigated to improve the formation of monomeric sugars in subsequent Enzymatic Hydrolysis for further bioconversion into ethanol. The solid fraction in the process stream, derived from a combined starch and ethanol factory, was rich in arabinose (21.1%), xylose (30.1%), and glucose (18.6%), in the form of polysaccharides. Various conditions of steam pretreatment (170-220 degrees C for 5-30 min) were evaluated, and their effect was assessed by Enzymatic Hydrolysis with 2 g of Celluclast + Ultraflo mixture/100 g of starch-free fiber (SFF) slurry at 5% dry matter (DM). The highest overall sugar yield for the combined steam pretreatment and Enzymatic Hydrolysis, 52 g/100 g of DM of SFF, corresponding to 74% of the theoretical, was achieved with pretreatment at 190 degrees C for 10 min followed by Enzymatic Hydrolysis.

  • Reduced inhibition of Enzymatic Hydrolysis of steam-pretreated softwood.
    Enzyme and Microbial Technology, 2001
    Co-Authors: Charlotte Tengborg, Mats Galbe, Guido Zacchi
    Abstract:

    Softwood constitutes the main source of lignocellulosic material in Sweden which can be used for ethanol production from renewable resources. To make the biomass-to-ethanol process more economically feasible, it is preferable to include the sugar-rich prehydrolysate, i.e. the liquid obtained after the pretreatment step, in the Enzymatic Hydrolysis of the solid fraction. This study shows that the prehydrolysate inhibits cellulose conversion in the Enzymatic Hydrolysis step. When the prehydrolysate was included in the Enzymatic Hydrolysis, the cellulose conversion was reduced by up to 36%. However, this inhibition can be overcome by fermentation of the prehydrolysate prior to Enzymatic Hydrolysis.