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Shuai Zang - One of the best experts on this subject based on the ideXlab platform.
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a multi walled carbon nanotubes poly l lysine modified enantioselective immunosensor for ofloxacin by using multi Enzyme labeled gold nanoflower as signal enhancer
Biosensors and Bioelectronics, 2015Co-Authors: Shuai Zang, Yingju Liu, Hongtao LeiAbstract:The enantioselective detection of trace amounts of ofloxacin is very important in many fields. In this work, an enantioselective and sensitive electrochemical immunosensor was constructed for the detection of chiral antibiotic ofloxacin based on a dual amplification strategy using multiwall carbon nanotubes-poly(L-lysine) as a matrix to immobilize the antigen and multi-Enzyme-Antibody functionalized gold nanoflowers as an electrochemical detection label. The fabrication process of the dual-amplified immunosensor was characterized by scanning electron microscopy, cyclic voltammogram and electrochemical impedance spectroscopy, respectively. After the optimization of the experimental conditions, a competitive immunoassay, i.e., the association ability with the corresponding Antibody between the captured antigen and free S-OFL or R-OFL in the solution, showed that the immunosensor exhibited a sensitive response to S-OFL in the range from 0.26 to 25.6 ng/mL with a detection limit of 0.15 ng/mL as well as a sensitive response to R-OFL in the range from 0.37 to 12.8 ng/mL with a detection limit of 0.30 ng/mL. Along with the acceptable sensitivity and stability, the S-OFL or R-OFL immunosensor showed selective ability to its corresponding enantiomer, suggesting this amplification strategy may hold a potential application in the detection of OFL in food or environment.
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A dual amplified electrochemical immunosensor for ofloxacin: Polypyrrole film-Au nanocluster as the matrix and multi-Enzyme-Antibody functionalized gold nanorod as the label
Electrochimica Acta, 2013Co-Authors: Shuai Zang, Jianli KangAbstract:a b s t r a c t In this work, an electrochemical immunosensor, basing on a dual signal amplified strategy by employing a biocompatible polypyrrole film-Au nanocluster matrix as a sensor platform and multi-Enzyme-Antibody functionalized gold nanorod as an electrochemical detection label, is established for sensitive detection of ofloxacin (OFL). Firstly, polypyrrole film and Au nanoclusters were progressively fabricated onto the surface of a glassy carbon electrode via electropolymerization and electrochemical deposition, respec- tively. Such PPy-Au nanocomposite modified electrode was used to immobilize OFL-OVA, blocked with the blocking reagent, and then associated with the corresponding Antibody. Secondly, gold nanorod (GNR) was synthesized to load horseradish peroxidase (HRP) and horseradish peroxidase-secondary Antibody (HRP-Ab2), and the resulting nanostructure (multi-HRP-GNR-Ab2) was applied as the detection label. The fabrication process of the ordered multilayer structure and immunosensor were characterized by scanning electron microscopy (SEM) and electrochemical measurements, respectively. Finally, based on a competitive immunoassay, i.e., the association ability with the corresponding Antibody between the captured antigen and free OFL in the solution, the fabricated immunosensor exhibited a sensitive response to OFL in the range from 0.08 to 410 ng/mL with a detection limit of 0.03 ng/mL. The current immunosensor exhibited good sensitivity, selectivity and long-term stability. This amplification strategy shows excellent promise for food safety monitoring of other antibiotics and a potential application in other immunosensors.
Jianli Kang - One of the best experts on this subject based on the ideXlab platform.
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A dual amplified electrochemical immunosensor for ofloxacin: Polypyrrole film-Au nanocluster as the matrix and multi-Enzyme-Antibody functionalized gold nanorod as the label
Electrochimica Acta, 2013Co-Authors: Shuai Zang, Jianli KangAbstract:a b s t r a c t In this work, an electrochemical immunosensor, basing on a dual signal amplified strategy by employing a biocompatible polypyrrole film-Au nanocluster matrix as a sensor platform and multi-Enzyme-Antibody functionalized gold nanorod as an electrochemical detection label, is established for sensitive detection of ofloxacin (OFL). Firstly, polypyrrole film and Au nanoclusters were progressively fabricated onto the surface of a glassy carbon electrode via electropolymerization and electrochemical deposition, respec- tively. Such PPy-Au nanocomposite modified electrode was used to immobilize OFL-OVA, blocked with the blocking reagent, and then associated with the corresponding Antibody. Secondly, gold nanorod (GNR) was synthesized to load horseradish peroxidase (HRP) and horseradish peroxidase-secondary Antibody (HRP-Ab2), and the resulting nanostructure (multi-HRP-GNR-Ab2) was applied as the detection label. The fabrication process of the ordered multilayer structure and immunosensor were characterized by scanning electron microscopy (SEM) and electrochemical measurements, respectively. Finally, based on a competitive immunoassay, i.e., the association ability with the corresponding Antibody between the captured antigen and free OFL in the solution, the fabricated immunosensor exhibited a sensitive response to OFL in the range from 0.08 to 410 ng/mL with a detection limit of 0.03 ng/mL. The current immunosensor exhibited good sensitivity, selectivity and long-term stability. This amplification strategy shows excellent promise for food safety monitoring of other antibiotics and a potential application in other immunosensors.
Hongtao Lei - One of the best experts on this subject based on the ideXlab platform.
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a multi walled carbon nanotubes poly l lysine modified enantioselective immunosensor for ofloxacin by using multi Enzyme labeled gold nanoflower as signal enhancer
Biosensors and Bioelectronics, 2015Co-Authors: Shuai Zang, Yingju Liu, Hongtao LeiAbstract:The enantioselective detection of trace amounts of ofloxacin is very important in many fields. In this work, an enantioselective and sensitive electrochemical immunosensor was constructed for the detection of chiral antibiotic ofloxacin based on a dual amplification strategy using multiwall carbon nanotubes-poly(L-lysine) as a matrix to immobilize the antigen and multi-Enzyme-Antibody functionalized gold nanoflowers as an electrochemical detection label. The fabrication process of the dual-amplified immunosensor was characterized by scanning electron microscopy, cyclic voltammogram and electrochemical impedance spectroscopy, respectively. After the optimization of the experimental conditions, a competitive immunoassay, i.e., the association ability with the corresponding Antibody between the captured antigen and free S-OFL or R-OFL in the solution, showed that the immunosensor exhibited a sensitive response to S-OFL in the range from 0.26 to 25.6 ng/mL with a detection limit of 0.15 ng/mL as well as a sensitive response to R-OFL in the range from 0.37 to 12.8 ng/mL with a detection limit of 0.30 ng/mL. Along with the acceptable sensitivity and stability, the S-OFL or R-OFL immunosensor showed selective ability to its corresponding enantiomer, suggesting this amplification strategy may hold a potential application in the detection of OFL in food or environment.
Shi-ying Lu - One of the best experts on this subject based on the ideXlab platform.
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Enzyme-Antibody dual labeled gold nanoparticles probe for ultrasensitive detection of κ-casein in bovine milk samples.
Biosensors and Bioelectronics, 2014Co-Authors: Yaru Li, Yulin Zhou, X.y. Meng, Yihua Zhang, Yudong Zhang, N.n. Wang, Pan Hu, Shi-ying LuAbstract:Abstract A dual labeled probe was synthesized by coating gold nanoparticles (AuNPs) with anti-κ-CN monoclonal Antibody (McAb) and horseradish peroxidase (HRP) Enzyme on their surface. The McAb was used as detector and HRP was used as label for signal amplification catalytically oxidize the substrate. AuNPs were used as bridges between the McAb and HRP. Based on the probe, an immunoassay was developed for ultrasensitive detection of κ-CN in bovine milk samples. The assay has a linear response range within 4.2–560 ng mL−1. The limit of detection (LOD) was 4.2 ng mL−1 which was 10 times lower than that of traditional McAb–HRP based ELISA. The recoveries of κ-CN from three brand bovine milk samples were from 95.8% to 111.0% that had a good correlation (R2=0.998) with those obtained by official standard Kjeldahl method. For higher sensitivity and as simple as the traditional ELISA, the developed immunoassay could provide an alternative approach for ultrasensitive detection of κ-CN in bovine milk sample.
J Boden - One of the best experts on this subject based on the ideXlab platform.
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plasma clearance of an Antibody Enzyme conjugate in adept by monoclonal anti Enzyme its effect on prodrug activation in vivo
British Journal of Cancer, 1995Co-Authors: G T Rogers, S K Sharma, Philip J Burke, R Koodie, J BodenAbstract:The effect of anti-Enzyme Antibody clearance on prodrug turnover in Antibody-directed Enzyme prodrug therapy (ADEPT) has been studied. Mice bearing LS174T xenografts were given localising carboxypeptidase G2 (CPG)2 conjugate (AEC) and 19 h later galactosylated anti-CPG2 Antibody (SB43-GAL). In regimen I prodrug was injected 5 h after SB43-GAL as previously described. In regimen 2 and 3 a shortened and extended clearance time was used in which prodrug was administered 0.5 h or 53 h after SB43-GAL respectively. Regimen 1 resulted in similar tumour and normal tissue levels of active drug to those of the control in which prodrug was given 72 h after AEC. SB43-GAL therefore accelerated clearance of Enzyme allowing early administration of prodrug. In regimen 2, very high active drug levels were found in the liver, showing removal of AEC from the blood followed by reactivation of Enzyme and extensive and rapid prodrug turnover. Active drug levels in tumour and blood reached similar peak levels to those of the control. Regimen 3 resulted in lower active drug levels in tissues, consistent with degradation and excretion of Enzyme. Regimen 3 also produced the best tumour to normal ratios for active drug. Residual prodrug in tumour was unaffected by SB43-GAL, showing the advantage of galactosylation in minimising inactivation of CPG2 in tumour. By contrast, residual prodrug in blood persisted for longer when SB43-GAL was used. Circulatory clearance of Enzyme with SB43-GAL allows prodrug to be administered expediently with reduced toxicity and with the prospect of increasing the dosage.