The Experts below are selected from a list of 45651 Experts worldwide ranked by ideXlab platform
Jianbin Zheng - One of the best experts on this subject based on the ideXlab platform.
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Recent progress in electrochemical sensing of cardiac troponin by using nanomaterial-induced Signal Amplification
Microchimica Acta, 2017Co-Authors: Qinglin Sheng, Xiujuan Qiao, Ming Zhou, Jianbin ZhengAbstract:Cardiac troponin (cTn) is a specific and sensitive biomarker for diagnosis of myocardial injury. Hence, numerous kinds of biosensors for cTn have been reported. Electrochemical methods possess inherent advantages over other kinds of sensors because they are specific, sensitive, and simple. By combining the advantages of electrochemical biosensors with those of nanomaterials, some interesting electrochemical biosensor for cTn can be obtained where the nanomaterials trigger substantial Signal Amplification. This review (with 101 refs.) summarizes the state of the art in electrochemical biosensing of cTn based on the use of nanomaterials. Following an introduction into the field, the use of nanomaterials in electrochemical sensing is briefly discussed. A next section covers strategies for Signal Amplification by using nanomaterials, with subsections on the use of nanowires, nanotubes, graphenes, and various other nanoparticles. The article concludes with a discussion of the prospects of nanomaterial-based Signal Amplification and on future research directions. Graphical abstractIllustration of electrochemical biosensing of cardiac troponin (cTn) with various kinds of nanomaterials, including nanowires, nanotubes, graphene and nanoparticles, as the Signal Amplification modules.
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Recent progress in electrochemical sensing of cardiac troponin by using nanomaterial-induced Signal Amplification
Mikrochimica Acta, 2017Co-Authors: Qinglin Sheng, Xiujuan Qiao, Ming Zhou, Jianbin ZhengAbstract:Cardiac troponin (cTn) is a specific and sensitive biomarker for diagnosis of myocardial injury. Hence, numerous kinds of biosensors for cTn have been reported. Electrochemical methods possess inherent advantages over other kinds of sensors because they are specific, sensitive, and simple. By combining the advantages of electrochemical biosensors with those of nanomaterials, some interesting electrochemical biosensor for cTn can be obtained where the nanomaterials trigger substantial Signal Amplification. This review (with 101 refs.) summarizes the state of the art in electrochemical biosensing of cTn based on the use of nanomaterials. Following an introduction into the field, the use of nanomaterials in electrochemical sensing is briefly discussed. A next section covers strategies for Signal Amplification by using nanomaterials, with subsections on the use of nanowires, nanotubes, graphenes, and various other nanoparticles. The article concludes with a discussion of the prospects of nanomaterial-based Signal Amplification and on future research directions.
Paul Komminoth - One of the best experts on this subject based on the ideXlab platform.
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Tyramide Signal Amplification for DNA and mRNA In Situ Hybridization
In Situ Hybridization Protocols, 2006Co-Authors: D A L Ian, Hewitson D. Tim, Ernst-jan M. Speel, Anton H N Hopman, Paul KomminothAbstract:In situ hybridization (ISH) has significantly advanced the study of gene structure and expression in cells and tissues, but its application is often limited by detection sensitivity. The introduction of Signal Amplification after ISH using tyramides has greatly advanced in situ detection methods that also are now applicable in routine diagnostics. In this chapter, we provide detailed step-by-step protocols for synthesis of biotinylated tyramides, multiple-target deoxyribonucleic acid-ISH on cell preparations, both DNA- and messenger ribonucleic acid (mRNA)-ISH on formalin-fixed, paraffin-embedded tissue sections, and tyramide Signal Amplification for Signal detection.
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Antigen retrieval, Signal Amplification and intensification in immunohistochemistry
Histochemistry and Cell Biology, 1996Co-Authors: Martin Werner, Reinhard Wasielewski, Paul KomminothAbstract:In this overview we emphasize new methods of improving immunohistochemical results in formaldehyde-fixed tissue samples. The benefit of heat-induced antigen retrieval in demasking of concealed epitopes is demonstrated. We provide guidance on the influence of heat-induced antigen retrieval in commonly applied monoclonal and polyconal antibodies. Moreover, we show the promising methods of Signal Amplification using biotinylated tyramine and Signal intensification of diaminobenzidine reaction products by metallic ions.
Qinglin Sheng - One of the best experts on this subject based on the ideXlab platform.
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Recent progress in electrochemical sensing of cardiac troponin by using nanomaterial-induced Signal Amplification
Microchimica Acta, 2017Co-Authors: Qinglin Sheng, Xiujuan Qiao, Ming Zhou, Jianbin ZhengAbstract:Cardiac troponin (cTn) is a specific and sensitive biomarker for diagnosis of myocardial injury. Hence, numerous kinds of biosensors for cTn have been reported. Electrochemical methods possess inherent advantages over other kinds of sensors because they are specific, sensitive, and simple. By combining the advantages of electrochemical biosensors with those of nanomaterials, some interesting electrochemical biosensor for cTn can be obtained where the nanomaterials trigger substantial Signal Amplification. This review (with 101 refs.) summarizes the state of the art in electrochemical biosensing of cTn based on the use of nanomaterials. Following an introduction into the field, the use of nanomaterials in electrochemical sensing is briefly discussed. A next section covers strategies for Signal Amplification by using nanomaterials, with subsections on the use of nanowires, nanotubes, graphenes, and various other nanoparticles. The article concludes with a discussion of the prospects of nanomaterial-based Signal Amplification and on future research directions. Graphical abstractIllustration of electrochemical biosensing of cardiac troponin (cTn) with various kinds of nanomaterials, including nanowires, nanotubes, graphene and nanoparticles, as the Signal Amplification modules.
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Recent progress in electrochemical sensing of cardiac troponin by using nanomaterial-induced Signal Amplification
Mikrochimica Acta, 2017Co-Authors: Qinglin Sheng, Xiujuan Qiao, Ming Zhou, Jianbin ZhengAbstract:Cardiac troponin (cTn) is a specific and sensitive biomarker for diagnosis of myocardial injury. Hence, numerous kinds of biosensors for cTn have been reported. Electrochemical methods possess inherent advantages over other kinds of sensors because they are specific, sensitive, and simple. By combining the advantages of electrochemical biosensors with those of nanomaterials, some interesting electrochemical biosensor for cTn can be obtained where the nanomaterials trigger substantial Signal Amplification. This review (with 101 refs.) summarizes the state of the art in electrochemical biosensing of cTn based on the use of nanomaterials. Following an introduction into the field, the use of nanomaterials in electrochemical sensing is briefly discussed. A next section covers strategies for Signal Amplification by using nanomaterials, with subsections on the use of nanowires, nanotubes, graphenes, and various other nanoparticles. The article concludes with a discussion of the prospects of nanomaterial-based Signal Amplification and on future research directions.
Jianping Lei - One of the best experts on this subject based on the ideXlab platform.
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Signal Amplification Using Nanomaterials for Biosensing
Springer Series on Chemical Sensors and Biosensors, 2013Co-Authors: Jianping LeiAbstract:Signal Amplification based on biofunctional nanomaterials has recently attracted considerable attention due to the need for ultrasensitive bioassays. Especially, most nanoscaled materials are biocompatible, which permits them to act in direct contact with the environment as carriers of biological recognition elements for obtaining lower and lower detection limit. In order to achieve the good performance for biosensing, two approaches including noncovalent interaction and covalent route have been introduced for the functionalization of nanomaterials with biomolecules. The biofunctional nanomaterials with the abilities of specific recognition and Signal triggering can be employed as not only excellent carriers, but also electronic and optical Signal tags to amplify the detection Signal. These advantages provide a new avenue to construct a sensitive and specific platform in nanobiosensing.
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Signal Amplification using functional nanomaterials for biosensing
Chemical Society Reviews, 2012Co-Authors: Jianping Lei, Huangxian JuAbstract:Signal Amplification based on biofunctional nanomaterials has recently attracted considerable attention due to the need for ultrasensitive bioassays and the trend towards miniaturized assays. The biofunctional nanomaterials can not only produce a synergic effect among catalytic activity, conductivity and biocompatibility to accelerate the Signal transduction, but also provide amplified recognition events by high loading of Signal tags, leading to a highly sensitive and specific biosensing. Most importantly, nanoscaled materials are in direct contact with the environment, which permits them to act as chemical and biological sensors in single-molecule detection of biomolecules. In this tutorial review, we will focus on recent significant advances in Signal Amplification strategies combining the cross-disciplines of chemistry, biology, and materials science, and highlight some elegant applications of biofunctional nanomaterials as excellent electronic or optical Signal tags in ultrasensitive bioanalysis. The biofunctional nanomaterials-based biosensing opens a series of concepts for basic research and offers new tools for detection of trace amounts of a wide variety of analytes in clinical, environmental, and industrial applications.
Eric V. Anslyn - One of the best experts on this subject based on the ideXlab platform.
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Self‐propagating Amplification reactions for molecular detection and Signal Amplification: Advantages, pitfalls, and challenges
Journal of Physical Organic Chemistry, 2018Co-Authors: Doron Shabat, Scott T. Phillips, Eric V. AnslynAbstract:Self-propagating cascade reactions are a recent development for chemo-sensing protocols. These cascade reactions, in principle, offer low limits of detection by virtue of exponential Signal Amplification, and are initiated by a specific, pre-planned molecular detection event. This combination of selectivity for a detection event followed by in situ Signal Amplification is achieved by exploitation of mechanistic organic chemistry, and thus has resulted in various chemo-sensing protocols that employ one or more reagents to achieve the desired selectivity and sensitivity for an assay. Species such as hydrogen peroxide, thiols, and fluoride, have been used as active reagents to initiate the first examples of self-propagating Signal Amplification reactions, although many other active reagents should be compatible with the approaches. A common feature of the reagents that support the self-propagating Signal Amplification reactions is the involvement of quinonemethide intermediates resulting from elimination of optical reporters and/or active reagents, where the latter propagates the Signal Amplification reaction. The early examples of these Amplification sequences, however, are slow to reach full Signal, thus leaving time for background reactions to generate non-specific Signals. This issue of background has limited practical applications of these self-propagating Signal Amplification reactions, as has challenging synthetic routes to the reagents, as well as the potential for other chemical species to interfere with the detection and Signal Amplification processes. Thus, the goal of this review is to summarize the progress of self-propagating Signal Amplification technology, identify the pitfalls of current designs, and by doing so, to stimulate future studies in this growing and promising research area.
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Self-Propagating Amplification Reactions for Molecular Detection and Signal Amplification: Advantages, Pitfalls, and Challenges.
Journal of Physical Organic Chemistry, 2018Co-Authors: Xiaolong Sun, Doron Shabat, Scott T. Phillips, Eric V. AnslynAbstract:Self-propagating cascade reactions are a recent development for chemo-sensing protocols. These cascade reactions, in principle, offer low limits of detection by virtue of exponential Signal Amplification, and are initiated by a specific, pre-planned molecular detection event. This combination of selectivity for a detection event followed by in situ Signal Amplification is achieved by exploitation of mechanistic organic chemistry, and thus has resulted in various chemo-sensing protocols that employ one or more reagents to achieve the desired selectivity and sensitivity for an assay. Species such as hydrogen peroxide, thiols, and fluoride, have been used as active reagents to initiate the first examples of self-propagating Signal Amplification reactions, although many other active reagents should be compatible with the approaches. A common feature of the reagents that support the self-propagating Signal Amplification reactions is the involvement of quinonemethide intermediates resulting from elimination of optical reporters and/or active reagents, where the latter propagates the Signal Amplification reaction. The early examples of these Amplification sequences, however, are slow to reach full Signal, thus leaving time for background reactions to generate non-specific Signals. This issue of background has limited practical applications of these self-propagating Signal Amplification reactions, as has challenging synthetic routes to the reagents, as well as the potential for other chemical species to interfere with the detection and Signal Amplification processes. Thus, the goal of this review is to summarize the progress of self-propagating Signal Amplification technology, identify the pitfalls of current designs, and by doing so, to stimulate future studies in this growing and promising research area.
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Signal Amplification by Allosteric Catalysis
Angewandte Chemie, 2006Co-Authors: Lei Zhu, Eric V. AnslynAbstract:In this article we unify a series of recent studies on bio- and chemosensors under a single Signaling strategy: Signal Amplification by allosteric catalysis (SAAC). The SAAC strategy mimics biological Signal transduction processes, where molecular recognition between an external Signal and a protein receptor is allosterically transduced into catalytically amplified chemical information (usually second messengers). Several recent biosensing and chemosensing studies apply this nature-inspired strategy by using engineered allosteric enzymes, ribozymes, or regulatable organic catalysts. The factors pertinent to achieving high sensitivity and specificity in SAAC strategies are analyzed. The authors believe that these early studies from a variety of research groups have opened up a new venue for the development of sensing technologies where molecular recognition and catalysis can be coupled for practical purposes.
