The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
Nigel S Scrutton - One of the best experts on this subject based on the ideXlab platform.
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Speeding up Enzyme Engineering computationally.
IUCrJ, 2016Co-Authors: Nigel S ScruttonAbstract:Can in silico Engineering speed up the delivery of biocatalysts for the burgeoning bioeconomy? In this issue, Kamerlin and coworkers introduce CADEE [Amrein et al. (2017), IUCrJ, 4, 50-64] - a framework for Computer-Aided Directed Evolution of Enzymes - that promises to lessen the burden on 'wet lab' enzymologists when optimizing biocatalysts using laboratory-based directed evolution methods.
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Enzyme Engineering toolbox a catalyst for change
Catalysis Science & Technology, 2013Co-Authors: Helen S Toogood, Nigel S ScruttonAbstract:Catalyst Engineering and process optimisation are critical to improve the efficiency, chiral purity and cost effectiveness of fine chemicals and pharmaceuticals manufacture. There is an increasing reliance on the use of Enzymes in chemical syntheses, which requires ‘fine-tuning’ of biocatalyst properties to optimise use under industrial conditions. Biocatalyst Engineering using random, semi-random and computational-based Enzyme redesign is beginning to impact significantly on industrial biocatalysis, enabling new and more efficient bio-based manufacturing. Here, approaches used and examples of biocatalyst design for industrial application are discussed alongside their relative merits, with particular emphasis on optimising the Old Yellow Enzyme family of Enzymes.
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Enzyme Engineering toolbox – a ‘catalyst’ for change
Catalysis Science & Technology, 2013Co-Authors: Helen S Toogood, Nigel S ScruttonAbstract:Catalyst Engineering and process optimisation are critical to improve the efficiency, chiral purity and cost effectiveness of fine chemicals and pharmaceuticals manufacture. There is an increasing reliance on the use of Enzymes in chemical syntheses, which requires ‘fine-tuning’ of biocatalyst properties to optimise use under industrial conditions. Biocatalyst Engineering using random, semi-random and computational-based Enzyme redesign is beginning to impact significantly on industrial biocatalysis, enabling new and more efficient bio-based manufacturing. Here, approaches used and examples of biocatalyst design for industrial application are discussed alongside their relative merits, with particular emphasis on optimising the Old Yellow Enzyme family of Enzymes.
Helen S Toogood - One of the best experts on this subject based on the ideXlab platform.
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Enzyme Engineering toolbox a catalyst for change
Catalysis Science & Technology, 2013Co-Authors: Helen S Toogood, Nigel S ScruttonAbstract:Catalyst Engineering and process optimisation are critical to improve the efficiency, chiral purity and cost effectiveness of fine chemicals and pharmaceuticals manufacture. There is an increasing reliance on the use of Enzymes in chemical syntheses, which requires ‘fine-tuning’ of biocatalyst properties to optimise use under industrial conditions. Biocatalyst Engineering using random, semi-random and computational-based Enzyme redesign is beginning to impact significantly on industrial biocatalysis, enabling new and more efficient bio-based manufacturing. Here, approaches used and examples of biocatalyst design for industrial application are discussed alongside their relative merits, with particular emphasis on optimising the Old Yellow Enzyme family of Enzymes.
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Enzyme Engineering toolbox – a ‘catalyst’ for change
Catalysis Science & Technology, 2013Co-Authors: Helen S Toogood, Nigel S ScruttonAbstract:Catalyst Engineering and process optimisation are critical to improve the efficiency, chiral purity and cost effectiveness of fine chemicals and pharmaceuticals manufacture. There is an increasing reliance on the use of Enzymes in chemical syntheses, which requires ‘fine-tuning’ of biocatalyst properties to optimise use under industrial conditions. Biocatalyst Engineering using random, semi-random and computational-based Enzyme redesign is beginning to impact significantly on industrial biocatalysis, enabling new and more efficient bio-based manufacturing. Here, approaches used and examples of biocatalyst design for industrial application are discussed alongside their relative merits, with particular emphasis on optimising the Old Yellow Enzyme family of Enzymes.
Jan-ytzen Van Der Meer - One of the best experts on this subject based on the ideXlab platform.
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The Generation and Exploitation of Protein Mutability Landscapes for Enzyme Engineering.
ChemBioChem, 2016Co-Authors: Jan-ytzen Van Der Meer, Lieuwe Biewenga, Gerrit J. PoelarendsAbstract:The increasing number of Enzyme applications in chemical synthesis calls for new Engineering methods to develop the biocatalysts of the future. An interesting concept in Enzyme Engineering is the generation of large-scale mutational data in order to chart protein mutability landscapes. These landscapes allow the important discrimination between beneficial mutations and those that are neutral or detrimental, thus providing detailed insight into sequence-function relationships. As such, mutability landscapes are a powerful tool with which to identify functional hotspots at any place in the amino acid sequence of an Enzyme. These hotspots can be used as targets for combinatorial mutagenesis to yield superior Enzymes with improved catalytic properties, stability, or even new enzymatic activities. The generation of mutability landscapes for multiple properties of one Enzyme provides the exciting opportunity to select mutations that are beneficial either for one or for several of these properties. This review presents an overview of the recent advances in the construction of mutability landscapes and discusses their importance for Enzyme Engineering.
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Mutability-landscape guided Enzyme Engineering: Improving the promiscuous C-C bond-forming activities of 4-oxalocrotonate tautomerase
2016Co-Authors: Jan-ytzen Van Der MeerAbstract:Enzymes do not only play a crucial role in nature, they are also increasingly used as biocatalysts for the production of complex molecules such as pharmaceuticals. However, some of the reactions that are widely used in organic synthesis have not been observed in biological systems. Hence, there is no biocatalytic alternative available for those important reactions. One way of generating Enzymes for these unnatural reactions is by exploiting the catalytic promiscuity of existing Enzymes. Jan Ytzen van der Meer has improved two promiscuous C-C bond-forming activities of the Enzyme 4-oxalocrotonate tautomerase (4-OT). For improving the promiscuous Michael-type addition activity of 4-OT, he first determined the effects of nearly all possible single amino acid substitutions on both activity and enantioselectivity. In the resulting mutability landscapes, all positive, neutral and detrimental effects of these mutations are displayed. Guided by these mutability landscapes of 4-OT, he then generated a set of highly active and enantiocomplementary ‘Michaelases’. These enantioselective Enzymes can be used for the convenient synthesis of both enantiomers of γ-nitroaldehydes, which are important precursors for pharmaceutically active GABA derivatives. The second promiscuous activity of 4-OT that was improved was the aldolase activity. Based on a similar approach, three residue positions were identified in 4-OT at which mutations led to a marked improvement of the promiscuous aldolase activity. Combinations of these mutations further improved this aldolase activity of 4-OT, allowing the enzymatic self- and cross-coupling of various aldehydes. Taken together, the work described in the thesis of Jan Ytzen van der Meer provides insights in the generation and application of mutability landscapes for efficient Enzyme Engineering and yielded comprehensive mutational data, which might be used as a unique training set to improve computational tools for Enzyme Engineering.
Saravanan Prabhu Nadarajan - One of the best experts on this subject based on the ideXlab platform.
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Unnatural amino acid mutagenesis-based Enzyme Engineering
Trends in Biotechnology, 2015Co-Authors: Yuvaraj Ravikumar, Saravanan Prabhu NadarajanAbstract:Traditional Enzyme Engineering relies on substituting one amino acid by one of the other 19 natural amino acids to change the functional properties of an Enzyme. However, incorporation of unnatural amino acids (UAAs) has been harnessed to engineer efficient Enzymes for biocatalysis. Residue-specific and site-specific in vivo incorporation methods are becoming the preferred approach for producing Enzymes with altered or improved functions. We describe the contribution of in vivo UAA incorporation methodologies to Enzyme Engineering as well as the future prospects for the field, including the integration of UAAs with other new advances in Enzyme Engineering.
A. K. Verma - One of the best experts on this subject based on the ideXlab platform.
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Enzyme Engineering for Enzyme Activity Improvement
Enzymes in Food Biotechnology, 2019Co-Authors: Ashutosh Dubey, A. K. VermaAbstract:Abstract Enzymes have several remarkable properties while offering incredible catalytic power for extensive diversity of cellular reactions, often with fine specificity toward substrates/products and remarkable regulation of catalytic activities. For commercial use, Enzymes with improved activities/stability are needed. The areas of biocatalyst improvement are implemented with the use of all available protein chemistry advancements. For modified Enzymes, four protein bioEngineering approaches are followed: (1) Structure-guided enzymatic protein Engineering for specific modifications, (2) Consensus designing to enhance multiEnzyme activity with changed localization or Enzyme folding, (3) Directed evolution by Enzyme mutation with the help of sequence libraries having preceding information regarding requisite properties and better variants selection, and (4) In silico or computational protein designing, a relatively unexplored but more valuable and fast-evolving device used to modify Enzyme activity. These approaches can be the solution for better utilization of Enzymes in commercial conditions where native Enzymes have functional shortcomings in terms of Enzyme stability and specificity in nonphysiological conditions. Thus, the Engineering of native Enzymes could be a tool to advance the enzymatic properties for commercial applications.