The Experts below are selected from a list of 8529 Experts worldwide ranked by ideXlab platform
Sun Ying - One of the best experts on this subject based on the ideXlab platform.
-
Eotaxin ccl11 and Eotaxin 2 ccl24 induce recruitment of eosinophils basophils neutrophils and macrophages as well as features of early and late phase allergic reactions following cutaneous injection in human atopic and nonatopic volunteers
Journal of Immunology, 2002Co-Authors: Andrew Menziesgow, Ian Sabroe, Victoria E L Stubbs, Dulce Soler, Sun Ying, T J WilliamsAbstract:Eotaxin and Eotaxin-2, acting through CCR3, are potent eosinophil chemoattractants both in vitro and in animal models. In this study we examined the capacity of Eotaxin and Eotaxin-2 to recruit eosinophils and other inflammatory cells in vivo in human atopic and nonatopic skin. Skin biopsies taken after intradermal injection of Eotaxin and Eotaxin-2 were examined by immunohistochemistry. Allergen- and diluent-challenged sites were used as positive and negative controls. Eotaxin and Eotaxin-2 produced a dose- and time-dependent local eosinophilia of comparable intensity in both atopic and nonatopic individuals. This was associated with an acute wheal and flare response at the site of injection and development of a cutaneous late phase reaction in a proportion of subjects. There was an accompanying decrease in mast cell numbers. Both chemokines also induced the accumulation of basophils and an unexpected early infiltration of neutrophils. Macrophages were prominent at the 24-h point. Although there was surface CCR3 expression on neutrophils in whole blood, we were unable to demonstrate any functional neutrophil responses to Eotaxin in vitro. Thus, intradermal injection of Eotaxin and Eotaxin-2 in humans induced infiltration of eosinophils and other inflammatory cells as well as changes consistent with CC chemokine-induced mast cell degranulation.
-
eosinophil chemotactic chemokines Eotaxin Eotaxin 2 rantes monocyte chemoattractant protein 3 mcp 3 and mcp 4 and c c chemokine receptor 3 expression in bronchial biopsies from atopic and nonatopic intrinsic asthmatics
Journal of Immunology, 1999Co-Authors: Sun Ying, Qiu Meng, Douglas S. Robinson, K Zeibecoglou, Alison J Macfarlane, Marc HumbertAbstract:Atopic (AA) and nonatopic (NAA) asthma are characterized by chronic inflammation and local tissue eosinophilia. Many C-C chemokines are potent eosinophil chemoattractants and act predominantly via the CCR3. We examined the expression of Eotaxin, Eotaxin-2, RANTES, monocyte chemoattractant protein-3 (MCP-3), MCP-4, and CCR3 in the bronchial mucosa from atopic (AA) and nonatopic (intrinsic; NAA) asthmatics and compared our findings with atopic (AC) and nonatopic nonasthmatic controls (NC). Cryostat sections were processed for immunohistochemistry (IHC), in situ hybridization (ISH), and double IHC/ISH. Compared with AC and NC, the numbers of EG2 + cells and the cells expressing mRNA for Eotaxin, Eotaxin-2, RANTES, MCP-3, MCP-4, and CCR3 were significantly increased in AA and NAA ( p + eosinophils in the bronchial tissue were also observed for both AA ( p p = 0.01). Moreover, in the total asthmatic group (AA + NAA) there was a significant inverse correlation between the expression of Eotaxin and that of the histamine PC 20 ( p + epithelial cells, CD31 + endothelial cells, and CD68 + macrophages were the major sources of Eotaxin, Eotaxin-2, RANTES, MCP-3, and MCP-4. There was no significantly different distribution of cells expressing mRNA for these chemokines between atopic and nonatopic asthma. These findings suggest that multiple C-C chemokines, acting at least in part via CCR3, contribute to bronchial eosinophilia in both atopic and nonatopic asthma.
-
c c chemokines in allergen induced late phase cutaneous responses in atopic subjects association of Eotaxin with early 6 hour eosinophils and of Eotaxin 2 and monocyte chemoattractant protein 4 with the later 24 hour tissue eosinophilia and relations
Journal of Immunology, 1999Co-Authors: Sun Ying, Luis T Barata, Alan R Mceuen, Mark G Buckley, Andrew F Walls, Qiu Meng, Douglas S. Robinson, Philip W. AskenaseAbstract:The relationship of expression of the C-C chemokines Eotaxin, Eotaxin 2, RANTES, monocyte chemoattractant protein-3 (MCP-3), and MCP-4 to the kinetics of infiltrating eosinophils, basophils, and other inflammatory cells was examined in allergen-induced, late-phase allergic reactions in the skin of human atopic subjects. EG2 + eosinophils peaked at 6 h and correlated significantly with Eotaxin mRNA and protein, whereas declining eosinophils at 24 h correlated significantly with Eotaxin-2 and MCP-4 mRNA. In contrast, no significant correlations were observed between BB1 + basophil infiltrates, which peaked at 24 h, and expression of Eotaxin, Eotaxin-2, RANTES, MCP-3, and MCP-4 or elastase + neutrophils (6-h peak), CD3 + and CD4 + T cells (24 h), and CD68 + macrophages (72 h). Furthermore, 83% of eosinophils, 40% of basophils, and 1% of CD3 + cells expressed the Eotaxin receptor CCR3, while Eotaxin protein was expressed by 43% of macrophages, 81% of endothelial cells, and 6% of T cells (6%). These data suggest that 1) Eotaxin has a role in the early 6-h recruitment of eosinophils, while Eotaxin-2 and MCP-4 appear to be involved in later 24-h infiltration of these CCR3 + cells; 2) different mechanisms may guide the early vs late eosinophilia; and 3) other chemokines and receptors may be involved in basophil accumulation of allergic tissue reactions in human skin.
Marc Humbert - One of the best experts on this subject based on the ideXlab platform.
-
eosinophil chemotactic chemokines Eotaxin Eotaxin 2 rantes monocyte chemoattractant protein 3 mcp 3 and mcp 4 and c c chemokine receptor 3 expression in bronchial biopsies from atopic and nonatopic intrinsic asthmatics
Journal of Immunology, 1999Co-Authors: Sun Ying, Qiu Meng, Douglas S. Robinson, K Zeibecoglou, Alison J Macfarlane, Marc HumbertAbstract:Atopic (AA) and nonatopic (NAA) asthma are characterized by chronic inflammation and local tissue eosinophilia. Many C-C chemokines are potent eosinophil chemoattractants and act predominantly via the CCR3. We examined the expression of Eotaxin, Eotaxin-2, RANTES, monocyte chemoattractant protein-3 (MCP-3), MCP-4, and CCR3 in the bronchial mucosa from atopic (AA) and nonatopic (intrinsic; NAA) asthmatics and compared our findings with atopic (AC) and nonatopic nonasthmatic controls (NC). Cryostat sections were processed for immunohistochemistry (IHC), in situ hybridization (ISH), and double IHC/ISH. Compared with AC and NC, the numbers of EG2 + cells and the cells expressing mRNA for Eotaxin, Eotaxin-2, RANTES, MCP-3, MCP-4, and CCR3 were significantly increased in AA and NAA ( p + eosinophils in the bronchial tissue were also observed for both AA ( p p = 0.01). Moreover, in the total asthmatic group (AA + NAA) there was a significant inverse correlation between the expression of Eotaxin and that of the histamine PC 20 ( p + epithelial cells, CD31 + endothelial cells, and CD68 + macrophages were the major sources of Eotaxin, Eotaxin-2, RANTES, MCP-3, and MCP-4. There was no significantly different distribution of cells expressing mRNA for these chemokines between atopic and nonatopic asthma. These findings suggest that multiple C-C chemokines, acting at least in part via CCR3, contribute to bronchial eosinophilia in both atopic and nonatopic asthma.
-
eosinophil chemotactic chemokines Eotaxin Eotaxin 2 rantes monocyte chemoattractant protein 3 mcp 3 and mcp 4 and c c chemokine receptor 3 expression in bronchial biopsies from atopic and nonatopic intrinsic asthmatics
Journal of Immunology, 1999Co-Authors: S Ying, Qiu Meng, K Zeibecoglou, Alison J Macfarlane, Marc Humbert, D S Robinson, A B KayAbstract:Atopic (AA) and nonatopic (NAA) asthma are characterized by chronic inflammation and local tissue eosinophilia. Many C-C chemokines are potent eosinophil chemoattractants and act predominantly via the CCR3. We examined the expression of Eotaxin, Eotaxin-2, RANTES, monocyte chemoattractant protein-3 (MCP-3), MCP-4, and CCR3 in the bronchial mucosa from atopic (AA) and nonatopic (intrinsic; NAA) asthmatics and compared our findings with atopic (AC) and nonatopic nonasthmatic controls (NC). Cryostat sections were processed for immunohistochemistry (IHC), in situ hybridization (ISH), and double IHC/ISH. Compared with AC and NC, the numbers of EG2+ cells and the cells expressing mRNA for Eotaxin, Eotaxin-2, RANTES, MCP-3, MCP-4, and CCR3 were significantly increased in AA and NAA (p < 0.01). Nonsignificant differences in these variants were observed between AA and NAA and between AC and NC. Significant correlations between the cells expressing Eotaxin or CCR3 and EG2+ eosinophils in the bronchial tissue were also observed for both AA (p < 0.01) and NAA (p = 0.01). Moreover, in the total asthmatic group (AA + NAA) there was a significant inverse correlation between the expression of Eotaxin and that of the histamine PC20 (p < 0.05). Sequential IHC/ISH showed that cytokeratin+ epithelial cells, CD31+ endothelial cells, and CD68+ macrophages were the major sources of Eotaxin, Eotaxin-2, RANTES, MCP-3, and MCP-4. There was no significantly different distribution of cells expressing mRNA for these chemokines between atopic and nonatopic asthma. These findings suggest that multiple C-C chemokines, acting at least in part via CCR3, contribute to bronchial eosinophilia in both atopic and nonatopic asthma.
Qiu Meng - One of the best experts on this subject based on the ideXlab platform.
-
eosinophil chemotactic chemokines Eotaxin Eotaxin 2 rantes monocyte chemoattractant protein 3 mcp 3 and mcp 4 and c c chemokine receptor 3 expression in bronchial biopsies from atopic and nonatopic intrinsic asthmatics
Journal of Immunology, 1999Co-Authors: S Ying, Qiu Meng, K Zeibecoglou, Alison J Macfarlane, Marc Humbert, D S Robinson, A B KayAbstract:Atopic (AA) and nonatopic (NAA) asthma are characterized by chronic inflammation and local tissue eosinophilia. Many C-C chemokines are potent eosinophil chemoattractants and act predominantly via the CCR3. We examined the expression of Eotaxin, Eotaxin-2, RANTES, monocyte chemoattractant protein-3 (MCP-3), MCP-4, and CCR3 in the bronchial mucosa from atopic (AA) and nonatopic (intrinsic; NAA) asthmatics and compared our findings with atopic (AC) and nonatopic nonasthmatic controls (NC). Cryostat sections were processed for immunohistochemistry (IHC), in situ hybridization (ISH), and double IHC/ISH. Compared with AC and NC, the numbers of EG2+ cells and the cells expressing mRNA for Eotaxin, Eotaxin-2, RANTES, MCP-3, MCP-4, and CCR3 were significantly increased in AA and NAA (p < 0.01). Nonsignificant differences in these variants were observed between AA and NAA and between AC and NC. Significant correlations between the cells expressing Eotaxin or CCR3 and EG2+ eosinophils in the bronchial tissue were also observed for both AA (p < 0.01) and NAA (p = 0.01). Moreover, in the total asthmatic group (AA + NAA) there was a significant inverse correlation between the expression of Eotaxin and that of the histamine PC20 (p < 0.05). Sequential IHC/ISH showed that cytokeratin+ epithelial cells, CD31+ endothelial cells, and CD68+ macrophages were the major sources of Eotaxin, Eotaxin-2, RANTES, MCP-3, and MCP-4. There was no significantly different distribution of cells expressing mRNA for these chemokines between atopic and nonatopic asthma. These findings suggest that multiple C-C chemokines, acting at least in part via CCR3, contribute to bronchial eosinophilia in both atopic and nonatopic asthma.
-
eosinophil chemotactic chemokines Eotaxin Eotaxin 2 rantes monocyte chemoattractant protein 3 mcp 3 and mcp 4 and c c chemokine receptor 3 expression in bronchial biopsies from atopic and nonatopic intrinsic asthmatics
Journal of Immunology, 1999Co-Authors: Sun Ying, Qiu Meng, Douglas S. Robinson, K Zeibecoglou, Alison J Macfarlane, Marc HumbertAbstract:Atopic (AA) and nonatopic (NAA) asthma are characterized by chronic inflammation and local tissue eosinophilia. Many C-C chemokines are potent eosinophil chemoattractants and act predominantly via the CCR3. We examined the expression of Eotaxin, Eotaxin-2, RANTES, monocyte chemoattractant protein-3 (MCP-3), MCP-4, and CCR3 in the bronchial mucosa from atopic (AA) and nonatopic (intrinsic; NAA) asthmatics and compared our findings with atopic (AC) and nonatopic nonasthmatic controls (NC). Cryostat sections were processed for immunohistochemistry (IHC), in situ hybridization (ISH), and double IHC/ISH. Compared with AC and NC, the numbers of EG2 + cells and the cells expressing mRNA for Eotaxin, Eotaxin-2, RANTES, MCP-3, MCP-4, and CCR3 were significantly increased in AA and NAA ( p + eosinophils in the bronchial tissue were also observed for both AA ( p p = 0.01). Moreover, in the total asthmatic group (AA + NAA) there was a significant inverse correlation between the expression of Eotaxin and that of the histamine PC 20 ( p + epithelial cells, CD31 + endothelial cells, and CD68 + macrophages were the major sources of Eotaxin, Eotaxin-2, RANTES, MCP-3, and MCP-4. There was no significantly different distribution of cells expressing mRNA for these chemokines between atopic and nonatopic asthma. These findings suggest that multiple C-C chemokines, acting at least in part via CCR3, contribute to bronchial eosinophilia in both atopic and nonatopic asthma.
-
c c chemokines in allergen induced late phase cutaneous responses in atopic subjects association of Eotaxin with early 6 hour eosinophils and of Eotaxin 2 and monocyte chemoattractant protein 4 with the later 24 hour tissue eosinophilia and relations
Journal of Immunology, 1999Co-Authors: Sun Ying, Luis T Barata, Alan R Mceuen, Mark G Buckley, Andrew F Walls, Qiu Meng, Douglas S. Robinson, Philip W. AskenaseAbstract:The relationship of expression of the C-C chemokines Eotaxin, Eotaxin 2, RANTES, monocyte chemoattractant protein-3 (MCP-3), and MCP-4 to the kinetics of infiltrating eosinophils, basophils, and other inflammatory cells was examined in allergen-induced, late-phase allergic reactions in the skin of human atopic subjects. EG2 + eosinophils peaked at 6 h and correlated significantly with Eotaxin mRNA and protein, whereas declining eosinophils at 24 h correlated significantly with Eotaxin-2 and MCP-4 mRNA. In contrast, no significant correlations were observed between BB1 + basophil infiltrates, which peaked at 24 h, and expression of Eotaxin, Eotaxin-2, RANTES, MCP-3, and MCP-4 or elastase + neutrophils (6-h peak), CD3 + and CD4 + T cells (24 h), and CD68 + macrophages (72 h). Furthermore, 83% of eosinophils, 40% of basophils, and 1% of CD3 + cells expressed the Eotaxin receptor CCR3, while Eotaxin protein was expressed by 43% of macrophages, 81% of endothelial cells, and 6% of T cells (6%). These data suggest that 1) Eotaxin has a role in the early 6-h recruitment of eosinophils, while Eotaxin-2 and MCP-4 appear to be involved in later 24-h infiltration of these CCR3 + cells; 2) different mechanisms may guide the early vs late eosinophilia; and 3) other chemokines and receptors may be involved in basophil accumulation of allergic tissue reactions in human skin.
Hun-taeg Chung - One of the best experts on this subject based on the ideXlab platform.
-
the association of Eotaxin 2 and Eotaxin 3 gene polymorphisms in a korean population with ulcerative colitis
Experimental and Molecular Medicine, 2005Co-Authors: Young Ran Park, Soo-cheon Chae, Suck Chei Choi, Soo Teik Lee, Kyung Suk Kim, Hun-taeg ChungAbstract:The Eotaxin gene family (Eotaxin, Eotaxin-2 and Eotaxin-3) have been implicated in the recruitment of eosinophils, basophiles and helper T (Th) 2 lymphocytes that is a central aspect of allergic disease. We previously suggested that Eo2+179T>C and Eo2 +275C>T of the Eotaxin-2, and Eo3 +2497T>G of the Eotaxin-3 were significantly associated with susceptibility to asthma. To determine whether the single nucleotide polymorphisms (SNPs) of Eotaxin-2 and Eotaxin-3 gene family are associated with the susceptibility of ulcerative colitis (UC), we analyzed the genotype of 119 patients with UC and 303 controls using single-base extension (SBE) method. We also calculated the haplotype frequencies among Eo2 +179T>C and Eo2 +275C >T of the Eotaxin-2 and Eo3 +2497T>G of the Eotaxin-3 in both control and UC patients. The genotype frequency of Eo2 +179T>C and Eo2 +275C>T between UC patients and controls were significantly different (P=0.006 and 0.022, respectively). The genotype and allele frequencies of EoA2497T>G in UC patients were not significantly different from those in the controls without UC patients. Our results suggest that Eo2 +179T>C and Eo2 +275C>T of Eotaxin-2 might be associated with the susceptibility of UC.
-
Eotaxin-3 gene polymorphisms are associated with rheumatoid arthritis in a Korean population.
Human Immunology, 2005Co-Authors: Soo-cheon Chae, Seung Cheol Shim, Young Ran Park, Hun-taeg ChungAbstract:The Eotaxin gene family (Eotaxin, Eotaxin-2, and Eotaxin-3) has been implicated in the recruitment of eosinophils, basophiles and Th2 lymphocytes that is a central aspect of allergic diseases. We previously suggested that Eo2+179T>C and Eo2+275C>T of the Eotaxin-2, and Eo3+2497T>G of the Eotaxin-3 were significantly associated with susceptibility to asthma. To precisely determine whether these single nucleotide polymorphisms (SNPs) are associated with susceptibility to autoimmune disease such as rheumatoid arthritis (RA) in Koreans, we analyzed the genotype and allele frequencies for four SNPs (Eo2+179T>C, Eo2+275C>T, Eo2+304A>C, and Eo2+1272A>G) of the Eotaxin-2, and three SNPs (Eo3+77C>T, Eo3+1577G>A, and Eo3+2497T>G) of the Eotaxin-3 by single-base extension method. Although the genotype and allele frequencies of the Eotaxin-2 SNPs gene between patients with RA and controls were not significantly different, the genotype and allele frequencies of the Eotaxin-3SNPs between them were significantly associated. The genotype frequencies of Eo3+1577G>A and Eo3+2497T>G in patients with RA were significantly different from those in the controls (p = 0.0001 and p < 0.0001, respectively). Our results strongly suggest that the polymorphisms of Eotaxin-3 might be associated with susceptibility to RA.
-
the suggestive association of Eotaxin 2 and Eotaxin 3 gene polymorphisms in korean population with allergic rhinitis
Immunogenetics, 2005Co-Authors: Soo-cheon Chae, Young Ran Park, Jae Hoon Lee, Hun-taeg ChungAbstract:The Eotaxin gene family (Eotaxin, Eotaxin-2 and Eotaxin-3) has been implicated in the recruitment of eosinophils, basophiles and Th2 lymphocytes that are central aspects of allergic diseases. To determine whether single-nucleotide polymorphisms (SNPs) of the Eotaxin-2 and Eotaxin-3 genes are associated with susceptibility to allergic rhinitis, we scanned 178 allergic rhinitis patients and 281 controls without allergic rhinitis using the direct sequencing and single-base extension (SBE) methods. We also calculated the haplotype frequencies between +179T>C and +275C>T of Eotaxin-2 and +2497T>G of Eotaxin-3 in both controls and allergic rhinitis patients. The haplotype frequency between controls and allergic rhinitis patients was suggestively associated (P=0.0001). The genotype frequencies of Eotaxin-3 +2497T>G in allergic rhinitis patients were suggestively different from those in non-allergic rhinitis controls (P=<0.0007). Our results strongly suggest that the SNP of Eotaxin-3 might be associated with susceptibility to allergic rhinitis.
A B Kay - One of the best experts on this subject based on the ideXlab platform.
-
eosinophil chemotactic chemokines Eotaxin Eotaxin 2 rantes monocyte chemoattractant protein 3 mcp 3 and mcp 4 and c c chemokine receptor 3 expression in bronchial biopsies from atopic and nonatopic intrinsic asthmatics
Journal of Immunology, 1999Co-Authors: S Ying, Qiu Meng, K Zeibecoglou, Alison J Macfarlane, Marc Humbert, D S Robinson, A B KayAbstract:Atopic (AA) and nonatopic (NAA) asthma are characterized by chronic inflammation and local tissue eosinophilia. Many C-C chemokines are potent eosinophil chemoattractants and act predominantly via the CCR3. We examined the expression of Eotaxin, Eotaxin-2, RANTES, monocyte chemoattractant protein-3 (MCP-3), MCP-4, and CCR3 in the bronchial mucosa from atopic (AA) and nonatopic (intrinsic; NAA) asthmatics and compared our findings with atopic (AC) and nonatopic nonasthmatic controls (NC). Cryostat sections were processed for immunohistochemistry (IHC), in situ hybridization (ISH), and double IHC/ISH. Compared with AC and NC, the numbers of EG2+ cells and the cells expressing mRNA for Eotaxin, Eotaxin-2, RANTES, MCP-3, MCP-4, and CCR3 were significantly increased in AA and NAA (p < 0.01). Nonsignificant differences in these variants were observed between AA and NAA and between AC and NC. Significant correlations between the cells expressing Eotaxin or CCR3 and EG2+ eosinophils in the bronchial tissue were also observed for both AA (p < 0.01) and NAA (p = 0.01). Moreover, in the total asthmatic group (AA + NAA) there was a significant inverse correlation between the expression of Eotaxin and that of the histamine PC20 (p < 0.05). Sequential IHC/ISH showed that cytokeratin+ epithelial cells, CD31+ endothelial cells, and CD68+ macrophages were the major sources of Eotaxin, Eotaxin-2, RANTES, MCP-3, and MCP-4. There was no significantly different distribution of cells expressing mRNA for these chemokines between atopic and nonatopic asthma. These findings suggest that multiple C-C chemokines, acting at least in part via CCR3, contribute to bronchial eosinophilia in both atopic and nonatopic asthma.