The Experts below are selected from a list of 45831 Experts worldwide ranked by ideXlab platform

L Mbonile - One of the best experts on this subject based on the ideXlab platform.

S S Kaukoranta - One of the best experts on this subject based on the ideXlab platform.

  • outbreak analysis and typing of mrsa isolates by automated repetitive sequence based pcr in a region with multiple strain types causing epidemics
    European Journal of Clinical Microbiology & Infectious Diseases, 2012
    Co-Authors: J J Hirvonen, Tanja Pasanen, Paivi Tissari, Saara Salmenlinna, Jaana Vuopio, S S Kaukoranta
    Abstract:

    The usefulness and performance of repetitive-sequence-based polymerase chain reaction (rep-PCR), the DiversiLab system, in the Epidemiological Surveillance for methicillin-resistant Staphylococcus aureus (MRSA) strain typing was assessed. MRSA isolates from five distinct outbreaks with precise Epidemiological data (n = 69) and from the culture collection of well-characterized MRSA strains (n = 132) consisting of 35 spa and 23 pulsed-field gel electrophoresis (PFGE) types were analyzed. The typing results of the DiversiLab system in outbreak analysis were compared to the spa and PFGE typing methods. The DiversiLab system proved to be a reliable tool for the rapid first-line typing of MRSA isolates, showing a good reliability in distinguishing MRSA strains in an area where several MRSA types were causing epidemics. This, however, required that the automatic clustering was combined with manual interpretation using the pattern overlay function when the strain types showing high similarity were clustered together. All outbreaks were distinguished with the DiversiLab system and the PFGE method, but not with the spa typing method. The overall discriminatory power of the DiversiLab system in differentiating diverse MRSA strains proved to be good. We also demonstrated that, in addition to the genetic relatedness analysis of MRSA strains, it is important to obtain accurate Epidemiological information in order to perform reliable Epidemiological Surveillance studies.

J J Hirvonen - One of the best experts on this subject based on the ideXlab platform.

  • outbreak analysis and typing of mrsa isolates by automated repetitive sequence based pcr in a region with multiple strain types causing epidemics
    European Journal of Clinical Microbiology & Infectious Diseases, 2012
    Co-Authors: J J Hirvonen, Tanja Pasanen, Paivi Tissari, Saara Salmenlinna, Jaana Vuopio, S S Kaukoranta
    Abstract:

    The usefulness and performance of repetitive-sequence-based polymerase chain reaction (rep-PCR), the DiversiLab system, in the Epidemiological Surveillance for methicillin-resistant Staphylococcus aureus (MRSA) strain typing was assessed. MRSA isolates from five distinct outbreaks with precise Epidemiological data (n = 69) and from the culture collection of well-characterized MRSA strains (n = 132) consisting of 35 spa and 23 pulsed-field gel electrophoresis (PFGE) types were analyzed. The typing results of the DiversiLab system in outbreak analysis were compared to the spa and PFGE typing methods. The DiversiLab system proved to be a reliable tool for the rapid first-line typing of MRSA isolates, showing a good reliability in distinguishing MRSA strains in an area where several MRSA types were causing epidemics. This, however, required that the automatic clustering was combined with manual interpretation using the pattern overlay function when the strain types showing high similarity were clustered together. All outbreaks were distinguished with the DiversiLab system and the PFGE method, but not with the spa typing method. The overall discriminatory power of the DiversiLab system in differentiating diverse MRSA strains proved to be good. We also demonstrated that, in addition to the genetic relatedness analysis of MRSA strains, it is important to obtain accurate Epidemiological information in order to perform reliable Epidemiological Surveillance studies.

Castillo-pabón, José O - One of the best experts on this subject based on the ideXlab platform.

  • "Proceso de eliminación del Tétanos neonatal en Colombia, 1989-2005"
    'Universidad Nacional de Colombia', 2020
    Co-Authors: Morón-duarte, Lina S, Castillo-pabón, José O
    Abstract:

    "Objective To describe the country's progress in eliminating neonatal tetanus based on the information obtained from epidemiologic Surveillance Material and Methods A retrospective descriptive study of the Epidemiological Surveillance of neonatal tetanus in Colombia in the period ranging from 1989 to 2005. Available information was consulted regarding confirmations of this event of interest for Public Health. Results During the study period, a total of 830 cases of neonatal tetanus were reported. In 1989, the rate of neonatal tetanus in Colombia was 0.22 per 1 000 live births. In 2005, this value was reduced to 0.01 cases per 1000 live births; a reduction of nearly 94 %. Conclusions Given the information provided by Epidemiological Surveillance, Colombia has achieved the goal of maintaining a rate less than 1 case per 1000 live births. Nevertheless, even one case anywhere in the country should be considered a failure of the health care system. © 2016, Universidad Nacional de Colombia. All rights reserved.

  • Epidemiological Surveillance of measles and German measles (rubella) within the context of the elimination plan: Colombia, 1995-2009
    'Universidad Nacional de Colombia', 2020
    Co-Authors: Morón-duarte, Lina S, Castillo-pabón, José O
    Abstract:

    "Describir el comportamiento de la vigilancia epidemiológica, con el fin de aportar evidencias sobre la interrupción de la circulación endémica de los virus del sarampión y la rubéola en el país. Métodos Estudio descriptivo retrospectivo de la vigilancia epidemiológica del sarampión y rubéola en Colombia para el período 1995-2009, a través de la consulta de información disponible de notificación en el aplicativo Measles Elimination Surveillance System (MESS), Sistema Nacional de Vigilancia en Salud Pública (SIVIGILA) y el Departamento Nacional de Estadísticas (DANE) para proyecciones poblacionales. La evaluación de la calidad de la vigilancia se hizo mediante los indicadores propuestos para la vigilancia integrada de sarampión y rubéola. Resultados En el período a estudio se notificaron 28 732 casos sospechosos, 66,15 % de sarampión y 33,8 % de rubéola, mayor número de casos notificados en 2002 (22,4 %); total de casos confirmados de sarampión entre 1995-2002 (n=495) y de rubéola (n=946) en todo el período. El promedio acumulado de los indicadores fue investigación oportuna con un comportamiento por debajo de 80,5 %, notificación semanal por encima de 80 %, toma de muestra adecuada en 93,7 %, y para recepción de muestra de 65,2 % para 1995-2006 y de 83,3 % para el periodo 2007-2009. Conclusiones De acuerdo con la información aportada por la vigilancia epidemiológica, el país cumple con 4 de los 7 indicadores propuestos para evaluar su calidad. Se evidencia que la circulación endémica de los virus de sarampión y rubéola en Colombia se encuentra interrumpida.""Describing the behavior of epidemiologic Surveillance regarding measles and German measles (rubella) to provide evidence about the interruption of the endemic circulation of triese viruses in Colombia.METHODS: This was a retrospective descriptive study of Epidemiological Surveillance for measles and German measles in Colombia from 1995 to 2009 by reviewing available notification information from the measles elimination Surveillance system (MESS), the Colombian Public Health Surveillance System (SIVIGILA) and the Colombian Statistics Department (DANE) for population projections. Surveillance quality was evaluated by using the indicators proposed for integrated measles and German measles Surveillance.RESULTS: 28,732 suspicious cases were notified during the study period (66.15 % concerned measles and 33.8 % German measles). The greatest number of notified cases occurred in 2002 (22.4 %); this was detected in the 6-11 month and 2-4 year-old groups. Confirmed measles cases amounted to 495 (1995-2002) and German measles to 946 (for the whole period). The cumulative average for indicators was as follows: timely research had a pattern below 80.5 %, weekly notification was above 80 %, suitable sampling was 93.7 % on average and sample reception was 65.2 % for 1995-2006 and 83.3 % for 2007-2009.CONCLUSIONS: According to the information compiled regarding Epidemiological Surveillance, Colombia complied with 4 of the 7 proposed indicators for quality evaluation; these showed active Surveillance having suitable indicator performance regarding laboratory and notification rate. They demonstrated interrupted endemic circulation of measles and German measles in Colombia.

  • Epidemiological Surveillance of measles and German measles (rubella) within the context of the elimination plan: Colombia, 1995-2009
    'FapUNIFESP (SciELO)', 2020
    Co-Authors: Morón-duarte, Lina S, Castillo-pabón, José O
    Abstract:

    Objetivo Describir el comportamiento de la vigilancia epidemiológica, con el fi n de aportar evidencias sobre la interrupción de la circulación endémica de los virus del sarampión y la rubéola en el país. Métodos Estudio descriptivo retrospectivo de la vigilancia epidemiológica del sarampión y rubéola en Colombia para el período 1995-2009, a través de la consulta de información disponible de notifi cación en el aplicativo Measles Elimination Surveillance System (MESS), Sistema Nacional de Vigilancia en Salud Pública (SIVIGILA) y el Departamento Nacional de Estadísticas (DANE) para proyecciones poblacionales. La evaluación de la calidad de la vigilancia se hizo mediante los indicadores propuestos para la vigilancia integrada de sarampión y rubéola. Resultados En el período a estudio se notifi caron 28 732 casos sospechosos, 66,15 % de sarampión y 33,8 % de rubéola, mayor número de casos notifi cados en 2002 (22,4 %); total de casos confi rmados de sarampión entre 1995-2002 (n=495) y de rubéola (n=946) en todo el período. El promedio acumulado de los indicadores fue investigación oportuna con un comportamiento por debajo de 80,5 %, notifi cación semanal por encima de 80 %, toma de muestra adecuada en 93,7 %, y para recepción de muestra de 65,2 % para 1995-2006 y de 83,3 % para el periodo 2007-2009. Conclusiones De acuerdo con la información aportada por la vigilancia epidemiológica, el país cumple con 4 de los 7 indicadores propuestos para evaluar su calidad. Se evidencia que la circulación endémica de los virus de sarampión y rubéola en Colombia se encuentra interrumpida.Objective Describing the behavior of epidemiologic Surveillance regarding measles and German measles (rubella) to provide evidence about the interruption of the endemic circulation of these viruses in Colombia. Methods This was a retrospective descriptive study of Epidemiological Surveillance for measles and German measles in Colombia from 1995 to 2009 by reviewing available notification information from the measles elimination Surveillance system (MESS), the Colombian Public Health Surveillance System (SIVIGILA) and the Colombian Statistics Department (DANE) for population projections. Surveillance quality was evaluated by using the indicators proposed for integrated measles and German measles Surveillance. Results 28,732 suspicious cases were notified during the study period (66.15 % concerned measles and 33.8 % German measles). The greatest number of notified cases occurred in 2002 (22.4 %); this was detected in the 6-11 month and 2-4 yearold groups. Confirmed measles cases amounted to 495 (1995-2002) and German measles to 946 (for the whole period). The cumulative average for indicators was as follows: timely research had a pattern below 80.5 %, weekly notification was above 80 %, suitable sampling was 93.7 % on average and sample reception was 65.2 % for 1995-2006 and 83.3 % for 2007-2009. Conclusions According to the information compiled regarding Epidemiological Surveillance, Colombia complied with 4 of the 7 proposed indicators for quality evaluation; these showed active Surveillance having suitable indicator performance regarding laboratory and notification rate. They demonstrated interrupted endemic circulation of measles and German measles in Colombia

Christophe Gantzer - One of the best experts on this subject based on the ideXlab platform.

  • Epidemiological Surveillance of sars cov 2 by genome quantification in wastewater applied to a city in the northeast of france comparison of ultrafiltration and protein precipitation based methods
    International Journal of Hygiene and Environmental Health, 2021
    Co-Authors: Isabelle Bertrand, Julie Challant, Helene Jeulin, Cedric Hartard, L Mathieu, Severine Lopez, Evelyne Schvoerer, Sophie Courtois, Christophe Gantzer
    Abstract:

    The aim of the present study was to develop a simple, sensitive, and specific approach to quantifying the SARS-CoV-2 genome in wastewater and to evaluate this approach as a means of Epidemiological Surveillance. Twelve wastewater samples were collected from a metropolitan area in north-eastern France during April and May 2020. In addition to the quantification of the SARS-CoV-2 genome, F-specific RNA phages of genogroup II (FRNAPH GGII), naturally present in wastewater, were used as an internal process control for the viral concentration and processing of RT-PCR inhibitors. A concentration method was required to allow the quantification of the SARS-CoV-2 genome over the longest possible period. A procedure combining ultrafiltration, phenol-chloroform-isoamyl alcohol purification, and the additional purification of the RNA extracts was chosen for the quantification of the SARS-CoV-2 genome in 100-mL wastewater samples. At the same time, the COVID-19 outbreak was evaluated through patients from the neighbouring University Hospital of Nancy, France. A regular decrease in the concentration of the SARS-CoV-2 genome from ~104 gc/L to ~102 gc/L of wastewater was observed over the eight weeks of the study, during which the population was placed under lockdown. The SARS-CoV-2 genome was even undetectable during one week in the second half of May and present but non-quantifiable in the last sample (28 May). A concordant circulation in the human community was highlighted by virological diagnosis using respiratory samples, which showed a decrease in the number of COVID-19 cases from 677 to 52 per week over the same period. The environmental Surveillance of COVID-19 using a reliable viral quantification procedure to test wastewater is a key approach. The real-time detection of viral genomes can allow us to predict and monitor the circulation of SARS-CoV-2 in clinical settings and survey the entire urban human population.