The Experts below are selected from a list of 324 Experts worldwide ranked by ideXlab platform
Thomas J Walters - One of the best experts on this subject based on the ideXlab platform.
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Epimysium and perimysium in suturing in skeletal muscle lacerations
Journal of Trauma-injury Infection and Critical Care, 2005Co-Authors: John F Kragh, Steven J Svoboda, Joseph C Wenke, John A Ward, Thomas J WaltersAbstract:Background: Direct muscle belly trauma is common. Selecting optimal methods for surgical repair of muscle disruption is difficult because reliable methods have not been established. Suturing tendon offers strong repairs, but Epimysium and perimysium, the connective tissues that coalesce to form tendons, offer unknown repair strength. The purpose of this study was to compare biomechanical properties of repaired muscle in transected muscle bellies with Epimysium and perimysium. Methods: The authors surgically repaired with figure-eight stitches in both Epimysium and perimysium groups. Individual stitches were placed in lacerated quadriceps bellies from a euthanized pig and were tensioned on a biomechanical machine. Maximum loads and strains were measured, and failure mechanisms were recorded. Results: Loads and strains for repairs with Epimysium were higher than those for repairs with perimysium. Failure mechanisms were significantly different between groups. Conclusion: These data showed that Epimysium incorporation into suturing improves capacity to bear forces compared with perimysium incorporation.
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the role of Epimysium in suturing skeletal muscle lacerations
Journal of The American College of Surgeons, 2005Co-Authors: John F Kragh, Steven J Svoboda, Joseph C Wenke, Daniel E Brooks, Terry G Bice, Thomas J WaltersAbstract:Background Direct muscle trauma is a common and disabling clinical problem. Surgical muscle repair is difficult to evaluate because reliable repair techniques have not been established scientifically. The purpose of this study was to assess the biomechanical properties of Epimysium, the collagenous tissue sheath that surrounds muscles in the body. Study design We surgically repaired transected porcine muscle bellies with and without Epimysium. For both groups, 25 figure-eight stitches in lacerated quadriceps bellies from a euthanatized pig were loaded under tension on a biomechanical machine (model 8521S, Instron Company). Maximum loads and strains were measured and mechanisms of failure recorded. Results The mean load for repairs with Epimysium (25.1 N) was significantly higher (p = 0.034) than that for repairs without Epimysium (21.2 N). The mean strain for repairs with Epimysium (10.4%) was significantly higher (p Conclusions These data showed that Epimysium incorporation into suturing improves the capacity of repairs to bear force. These findings fill a knowledge gap and may improve outcomes of muscle suturing. By focusing the experiment on biomechanical properties of muscle stitching, this study showed the key role Epimysium plays in muscle suturing.
Alan S Wineman - One of the best experts on this subject based on the ideXlab platform.
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age related changes in the mechanical properties of the Epimysium in skeletal muscles of rats
Journal of Biomechanics, 2008Co-Authors: Yingxin Gao, Tatiana Y Kostrominova, John A Faulkner, Alan S WinemanAbstract:Skeletal muscle is composed of muscle fibers and an extracellular matrix (ECM). The collagen fiber network of the ECM is a major contributor to the passive force of skeletal muscles at high strain. We investigated the effect of aging on the biomechanical and structural properties of Epimysium of the tibialis anterior muscles (TBA) of rats to understand the mechanisms responsible for the age-related changes. The biomechanical properties were tested directly in vitro by uniaxial extension of Epimysium. The presence of age-related changes in the arrangement and size of the collagen fibrils in the Epimysium was examined by scanning electron microscopy (SEM). A mathematical model was subsequently developed based on the structure-function relationships that predicted the compliance of the Epimysium. Biomechanically, the Epimysium from old rats was much stiffer than that of the young rats. No differences were found in the ultrastructure and thickness of the Epimysium or size of the collagen fibrils between young and old rats. The changes in the arrangement and size of the collagen fibrils do not appear to be the principal cause of the increased stiffness of the Epimysium from the old rats. Other changes in the structural composition of the Epimysium from old rats likely has a strong effect on the increased stiffness. The age-related increase in the stiffness of the Epimysium could play an important role in the impaired lateral force transmission in the muscles of the elderly.
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micromechanical modeling of the Epimysium of the skeletal muscles
Journal of Biomechanics, 2008Co-Authors: Yingxin Gao, Tatiana Y Kostrominova, John A Faulkner, Anthony M Waas, Alan S WinemanAbstract:A micromechanical model has been developed to investigate the mechanical properties of the Epimysium. In the present model, the collagen fibers in the Epimysium are embedded randomly in the ground substance. Two parallel wavy collagen fibers and the surrounding ground substance are used as the repeat unit (unit cell), and the Epimysium is considered as an aggregate of unit cells. Each unit cell is distributed in the Epimysium with some different angle to the muscle fiber direction. The model allows the progressive straightening of the collagen fiber as well as the effects of fiber reorientation. The predictions of the model compare favorably against experiment. The effects of the collagen fiber volume fraction, collagen fiber waviness at the rest length and the mechanical properties of the collagen fibers and the ground substance are analyzed. This model allows the analysis of mechanical behavior of most soft tissues if appropriate experimental data are available.
Tatiana Y Kostrominova - One of the best experts on this subject based on the ideXlab platform.
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age related changes in the mechanical properties of the Epimysium in skeletal muscles of rats
Journal of Biomechanics, 2008Co-Authors: Yingxin Gao, Tatiana Y Kostrominova, John A Faulkner, Alan S WinemanAbstract:Skeletal muscle is composed of muscle fibers and an extracellular matrix (ECM). The collagen fiber network of the ECM is a major contributor to the passive force of skeletal muscles at high strain. We investigated the effect of aging on the biomechanical and structural properties of Epimysium of the tibialis anterior muscles (TBA) of rats to understand the mechanisms responsible for the age-related changes. The biomechanical properties were tested directly in vitro by uniaxial extension of Epimysium. The presence of age-related changes in the arrangement and size of the collagen fibrils in the Epimysium was examined by scanning electron microscopy (SEM). A mathematical model was subsequently developed based on the structure-function relationships that predicted the compliance of the Epimysium. Biomechanically, the Epimysium from old rats was much stiffer than that of the young rats. No differences were found in the ultrastructure and thickness of the Epimysium or size of the collagen fibrils between young and old rats. The changes in the arrangement and size of the collagen fibrils do not appear to be the principal cause of the increased stiffness of the Epimysium from the old rats. Other changes in the structural composition of the Epimysium from old rats likely has a strong effect on the increased stiffness. The age-related increase in the stiffness of the Epimysium could play an important role in the impaired lateral force transmission in the muscles of the elderly.
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micromechanical modeling of the Epimysium of the skeletal muscles
Journal of Biomechanics, 2008Co-Authors: Yingxin Gao, Tatiana Y Kostrominova, John A Faulkner, Anthony M Waas, Alan S WinemanAbstract:A micromechanical model has been developed to investigate the mechanical properties of the Epimysium. In the present model, the collagen fibers in the Epimysium are embedded randomly in the ground substance. Two parallel wavy collagen fibers and the surrounding ground substance are used as the repeat unit (unit cell), and the Epimysium is considered as an aggregate of unit cells. Each unit cell is distributed in the Epimysium with some different angle to the muscle fiber direction. The model allows the progressive straightening of the collagen fiber as well as the effects of fiber reorientation. The predictions of the model compare favorably against experiment. The effects of the collagen fiber volume fraction, collagen fiber waviness at the rest length and the mechanical properties of the collagen fibers and the ground substance are analyzed. This model allows the analysis of mechanical behavior of most soft tissues if appropriate experimental data are available.
Lisa M Larkin - One of the best experts on this subject based on the ideXlab platform.
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engineered skeletal muscle units for repair of volumetric muscle loss in the tibialis anterior muscle of a rat
Tissue Engineering Part A, 2014Co-Authors: Keith W Vandusen, Brian C Syverud, Michael L Williams, Lisa M LarkinAbstract:Volumetric muscle loss (VML) is the traumatic, degenerative, or surgical loss of muscle tissue, which may result in function loss and physical deformity. To date, clinical treatments for VML—the reflected muscle flap or transferred muscle graft—are limited by tissue availability and donor site morbidity. To address the need for more innovative skeletal muscle repair options, our laboratory has developed scaffoldless tissue-engineered skeletal muscle units (SMUs), multiphasic tissue constructs composed of engineered skeletal muscle with engineered bone-tendon ends, myotendinous junctions, and entheses, which in vitro can produce force both spontaneously and in response to electrical stimulation. Though phenotypically immature in vitro, we have shown that following 1 week of implantation in an ectopic site, our muscle constructs develop vascularization and innervation, an Epimysium-like outer layer of connective tissue, an increase in myosin protein content, formation of myofibers, and increased force production. These findings suggest that our engineered muscle tissue survives implantation and develops the interfaces necessary to advance the phenotype toward adult muscle. The purpose of this study was to evaluate the potential of our SMUs to restore muscle tissue to sites of acute VML. Our results indicate that our SMUs continue to mature in vivo with longer recovery times and have the potential to repair VML sites by providing additional muscle fibers to damaged muscles. We conclude from this study that our SMUs have the potential to restore lost tissue volume in cases of acute VML.
John F Kragh - One of the best experts on this subject based on the ideXlab platform.
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Epimysium and perimysium in suturing in skeletal muscle lacerations
Journal of Trauma-injury Infection and Critical Care, 2005Co-Authors: John F Kragh, Steven J Svoboda, Joseph C Wenke, John A Ward, Thomas J WaltersAbstract:Background: Direct muscle belly trauma is common. Selecting optimal methods for surgical repair of muscle disruption is difficult because reliable methods have not been established. Suturing tendon offers strong repairs, but Epimysium and perimysium, the connective tissues that coalesce to form tendons, offer unknown repair strength. The purpose of this study was to compare biomechanical properties of repaired muscle in transected muscle bellies with Epimysium and perimysium. Methods: The authors surgically repaired with figure-eight stitches in both Epimysium and perimysium groups. Individual stitches were placed in lacerated quadriceps bellies from a euthanized pig and were tensioned on a biomechanical machine. Maximum loads and strains were measured, and failure mechanisms were recorded. Results: Loads and strains for repairs with Epimysium were higher than those for repairs with perimysium. Failure mechanisms were significantly different between groups. Conclusion: These data showed that Epimysium incorporation into suturing improves capacity to bear forces compared with perimysium incorporation.
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the role of Epimysium in suturing skeletal muscle lacerations
Journal of The American College of Surgeons, 2005Co-Authors: John F Kragh, Steven J Svoboda, Joseph C Wenke, Daniel E Brooks, Terry G Bice, Thomas J WaltersAbstract:Background Direct muscle trauma is a common and disabling clinical problem. Surgical muscle repair is difficult to evaluate because reliable repair techniques have not been established scientifically. The purpose of this study was to assess the biomechanical properties of Epimysium, the collagenous tissue sheath that surrounds muscles in the body. Study design We surgically repaired transected porcine muscle bellies with and without Epimysium. For both groups, 25 figure-eight stitches in lacerated quadriceps bellies from a euthanatized pig were loaded under tension on a biomechanical machine (model 8521S, Instron Company). Maximum loads and strains were measured and mechanisms of failure recorded. Results The mean load for repairs with Epimysium (25.1 N) was significantly higher (p = 0.034) than that for repairs without Epimysium (21.2 N). The mean strain for repairs with Epimysium (10.4%) was significantly higher (p Conclusions These data showed that Epimysium incorporation into suturing improves the capacity of repairs to bear force. These findings fill a knowledge gap and may improve outcomes of muscle suturing. By focusing the experiment on biomechanical properties of muscle stitching, this study showed the key role Epimysium plays in muscle suturing.