The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform
Swati Patil - One of the best experts on this subject based on the ideXlab platform.
-
Immunohistochemical evaluation of expression pattern of p53, p63, and p73 in Epithelial Dysplasia
Journal of Datta Meghe Institute of Medical Sciences University, 2018Co-Authors: Deepali P. Mohite, Minal Chaudhary, Swati Patil, Alka Hande, Rolly Gupta, Prakash M. Mohite, Madhuri GawandeAbstract:Objective: The objective of this study was to evaluate the expression pattern of p53, p63, and p73 in different grades of Epithelial Dysplasia through immunohistochemical (IHC). Materials and Methods: 30 cases of Epithelial Dysplasia and 10 samples of normal buccal mucosa were included in the study. The cases with Epithelial Dysplasia were classified as mild, moderate and severe. Immunohistochemical staining was carried out using monoclonal mouse anti-human antibody against p53, p63, and rabbit polyclonal antibody against p73. Labelling index was calculated. The numbers of positively stained nuclei were expressed as a percentage of the total number counted for individual layer and in complete epithelium. The number of cells positive for p63 protein immunoexpression was higher than that for p53. Significant difference in positivity was observed with respect to p53 and p63 amongst all the grades of Dysplasia. In severe Dysplasia, significantly higher expression of p63 was observed. Expression of p53 was significantly higher in severe Dysplasia than mild Dysplasia and normal tissue. p73 was observed to be non significant amongst all grades of Dysplasia and in normal tissue. Significant positive correlation was noted between p63 and p53 in severe Dysplasia. Study Design: Thirty cases of Epithelial Dysplasia and 10 samples of normal buccal mucosa were included in the study. The cases with Epithelial Dysplasia were classified as mild, moderate, and severe. IHC staining was carried out using monoclonal mouse anti-human antibody against p53, p63, and rabbit polyclonal antibody against p73. Labeling index was calculated. The numbers of positively stained nuclei were expressed as a percentage of the total number counted for the individual layer and in the complete epithelium. The number of cells positive for p63 protein immunoexpression was higher than that for p53. Significant difference in positivity was observed with respect to p53 and p63 among all the grades of Dysplasia. In severe Dysplasia, significantly higher expression of p63 was observed. Expression of p53 was significantly higher in severe Dysplasia than mild Dysplasia and normal tissue. p73 was observed to be nonsignificant among all grades of Dysplasia and in normal tissue. Significant positive correlation was noted between p63 and p53 in severe Dysplasia. Results and Conclusion: Our results indicate that increase in the severity of Dysplasia correlates with increase in expression of p53 and p63. Increased immunoexpression of p53 and p63 may help in identifying those cases of Epithelial Dysplasia which can transform into carcinomas. p53 alongwith p63 is a better indicator of progression of Dysplasia toward malignancy rather than p53/p63 alone. This can serve as a useful prognostic indicator and also an indicator of treatment outcome.
-
Comparison of Myofibroblasts Expression in Oral Squamous Cell Carcinoma, Verrucous Carcinoma, High Risk Epithelial Dysplasia, Low Risk Epithelial Dysplasia and Normal Oral Mucosa
Head and Neck Pathology, 2012Co-Authors: Minal Chaudhary, Amol Ramchandra Gadbail, Gaurav Vidhale, Mugdha P. Mankar (gadbail), Shailesh M. Gondivkar, Madhuri Gawande, Swati PatilAbstract:The aim was to evaluate and compare the presence of myofibroblasts in oral squamous cell carcinoma (OSCC), verrucous carcinoma (VC), high-risk Epithelial Dysplasia (HRED), low-risk Epithelial Dysplasia (LRED), and normal oral mucosa (NOM). The study consisted of 37 OSCC, 15 VC, 15 HRED, 15 LRED and 15 NOM. α-smooth muscle actin (α-SMA) antibody was used to identify myofibroblasts. The α-SMA expression was not observed in NOM and LRED. The α-SMA was expressed in 97.29% of OSCC, 86.66% of VC, 46.66 % of HRED. The α-SMA expression was significantly higher in OSCC than VC ( p = 0.023) and HRED ( p
-
comparison of myofibroblasts expression in oral squamous cell carcinoma verrucous carcinoma high risk Epithelial Dysplasia low risk Epithelial Dysplasia and normal oral mucosa
Head and Neck Pathology, 2012Co-Authors: Minal Chaudhary, Amol Ramchandra Gadbail, Gaurav Vidhale, Shailesh M. Gondivkar, Madhuri Gawande, Mugdha Mankar, Swati PatilAbstract:The aim was to evaluate and compare the presence of myofibroblasts in oral squamous cell carcinoma (OSCC), verrucous carcinoma (VC), high-risk Epithelial Dysplasia (HRED), low-risk Epithelial Dysplasia (LRED), and normal oral mucosa (NOM). The study consisted of 37 OSCC, 15 VC, 15 HRED, 15 LRED and 15 NOM. α-smooth muscle actin (α-SMA) antibody was used to identify myofibroblasts. The α-SMA expression was not observed in NOM and LRED. The α-SMA was expressed in 97.29% of OSCC, 86.66% of VC, 46.66 % of HRED. The α-SMA expression was significantly higher in OSCC than VC (p = 0.023) and HRED (p < 0.000). The α-SMA expression was significantly higher in VC than HRED (p = 0.043). Myofibroblastic expression, as highlighted by α-SMA, is undetectable in NOM and LRED but increases as the disease progresses from potentially malignant disorders, as HRED to VC to invasive OSCC. Thus, proliferation of myofibroblasts may be used as a stromal marker of oral premalignancy and malignancy.
Minal Chaudhary - One of the best experts on this subject based on the ideXlab platform.
-
Immunohistochemical evaluation of expression pattern of p53, p63, and p73 in Epithelial Dysplasia
Journal of Datta Meghe Institute of Medical Sciences University, 2018Co-Authors: Deepali P. Mohite, Minal Chaudhary, Swati Patil, Alka Hande, Rolly Gupta, Prakash M. Mohite, Madhuri GawandeAbstract:Objective: The objective of this study was to evaluate the expression pattern of p53, p63, and p73 in different grades of Epithelial Dysplasia through immunohistochemical (IHC). Materials and Methods: 30 cases of Epithelial Dysplasia and 10 samples of normal buccal mucosa were included in the study. The cases with Epithelial Dysplasia were classified as mild, moderate and severe. Immunohistochemical staining was carried out using monoclonal mouse anti-human antibody against p53, p63, and rabbit polyclonal antibody against p73. Labelling index was calculated. The numbers of positively stained nuclei were expressed as a percentage of the total number counted for individual layer and in complete epithelium. The number of cells positive for p63 protein immunoexpression was higher than that for p53. Significant difference in positivity was observed with respect to p53 and p63 amongst all the grades of Dysplasia. In severe Dysplasia, significantly higher expression of p63 was observed. Expression of p53 was significantly higher in severe Dysplasia than mild Dysplasia and normal tissue. p73 was observed to be non significant amongst all grades of Dysplasia and in normal tissue. Significant positive correlation was noted between p63 and p53 in severe Dysplasia. Study Design: Thirty cases of Epithelial Dysplasia and 10 samples of normal buccal mucosa were included in the study. The cases with Epithelial Dysplasia were classified as mild, moderate, and severe. IHC staining was carried out using monoclonal mouse anti-human antibody against p53, p63, and rabbit polyclonal antibody against p73. Labeling index was calculated. The numbers of positively stained nuclei were expressed as a percentage of the total number counted for the individual layer and in the complete epithelium. The number of cells positive for p63 protein immunoexpression was higher than that for p53. Significant difference in positivity was observed with respect to p53 and p63 among all the grades of Dysplasia. In severe Dysplasia, significantly higher expression of p63 was observed. Expression of p53 was significantly higher in severe Dysplasia than mild Dysplasia and normal tissue. p73 was observed to be nonsignificant among all grades of Dysplasia and in normal tissue. Significant positive correlation was noted between p63 and p53 in severe Dysplasia. Results and Conclusion: Our results indicate that increase in the severity of Dysplasia correlates with increase in expression of p53 and p63. Increased immunoexpression of p53 and p63 may help in identifying those cases of Epithelial Dysplasia which can transform into carcinomas. p53 alongwith p63 is a better indicator of progression of Dysplasia toward malignancy rather than p53/p63 alone. This can serve as a useful prognostic indicator and also an indicator of treatment outcome.
-
Comparison of Myofibroblasts Expression in Oral Squamous Cell Carcinoma, Verrucous Carcinoma, High Risk Epithelial Dysplasia, Low Risk Epithelial Dysplasia and Normal Oral Mucosa
Head and Neck Pathology, 2012Co-Authors: Minal Chaudhary, Amol Ramchandra Gadbail, Gaurav Vidhale, Mugdha P. Mankar (gadbail), Shailesh M. Gondivkar, Madhuri Gawande, Swati PatilAbstract:The aim was to evaluate and compare the presence of myofibroblasts in oral squamous cell carcinoma (OSCC), verrucous carcinoma (VC), high-risk Epithelial Dysplasia (HRED), low-risk Epithelial Dysplasia (LRED), and normal oral mucosa (NOM). The study consisted of 37 OSCC, 15 VC, 15 HRED, 15 LRED and 15 NOM. α-smooth muscle actin (α-SMA) antibody was used to identify myofibroblasts. The α-SMA expression was not observed in NOM and LRED. The α-SMA was expressed in 97.29% of OSCC, 86.66% of VC, 46.66 % of HRED. The α-SMA expression was significantly higher in OSCC than VC ( p = 0.023) and HRED ( p
-
comparison of myofibroblasts expression in oral squamous cell carcinoma verrucous carcinoma high risk Epithelial Dysplasia low risk Epithelial Dysplasia and normal oral mucosa
Head and Neck Pathology, 2012Co-Authors: Minal Chaudhary, Amol Ramchandra Gadbail, Gaurav Vidhale, Shailesh M. Gondivkar, Madhuri Gawande, Mugdha Mankar, Swati PatilAbstract:The aim was to evaluate and compare the presence of myofibroblasts in oral squamous cell carcinoma (OSCC), verrucous carcinoma (VC), high-risk Epithelial Dysplasia (HRED), low-risk Epithelial Dysplasia (LRED), and normal oral mucosa (NOM). The study consisted of 37 OSCC, 15 VC, 15 HRED, 15 LRED and 15 NOM. α-smooth muscle actin (α-SMA) antibody was used to identify myofibroblasts. The α-SMA expression was not observed in NOM and LRED. The α-SMA was expressed in 97.29% of OSCC, 86.66% of VC, 46.66 % of HRED. The α-SMA expression was significantly higher in OSCC than VC (p = 0.023) and HRED (p < 0.000). The α-SMA expression was significantly higher in VC than HRED (p = 0.043). Myofibroblastic expression, as highlighted by α-SMA, is undetectable in NOM and LRED but increases as the disease progresses from potentially malignant disorders, as HRED to VC to invasive OSCC. Thus, proliferation of myofibroblasts may be used as a stromal marker of oral premalignancy and malignancy.
Amol Ramchandra Gadbail - One of the best experts on this subject based on the ideXlab platform.
-
Comparison of Myofibroblasts Expression in Oral Squamous Cell Carcinoma, Verrucous Carcinoma, High Risk Epithelial Dysplasia, Low Risk Epithelial Dysplasia and Normal Oral Mucosa
Head and Neck Pathology, 2012Co-Authors: Minal Chaudhary, Amol Ramchandra Gadbail, Gaurav Vidhale, Mugdha P. Mankar (gadbail), Shailesh M. Gondivkar, Madhuri Gawande, Swati PatilAbstract:The aim was to evaluate and compare the presence of myofibroblasts in oral squamous cell carcinoma (OSCC), verrucous carcinoma (VC), high-risk Epithelial Dysplasia (HRED), low-risk Epithelial Dysplasia (LRED), and normal oral mucosa (NOM). The study consisted of 37 OSCC, 15 VC, 15 HRED, 15 LRED and 15 NOM. α-smooth muscle actin (α-SMA) antibody was used to identify myofibroblasts. The α-SMA expression was not observed in NOM and LRED. The α-SMA was expressed in 97.29% of OSCC, 86.66% of VC, 46.66 % of HRED. The α-SMA expression was significantly higher in OSCC than VC ( p = 0.023) and HRED ( p
-
comparison of myofibroblasts expression in oral squamous cell carcinoma verrucous carcinoma high risk Epithelial Dysplasia low risk Epithelial Dysplasia and normal oral mucosa
Head and Neck Pathology, 2012Co-Authors: Minal Chaudhary, Amol Ramchandra Gadbail, Gaurav Vidhale, Shailesh M. Gondivkar, Madhuri Gawande, Mugdha Mankar, Swati PatilAbstract:The aim was to evaluate and compare the presence of myofibroblasts in oral squamous cell carcinoma (OSCC), verrucous carcinoma (VC), high-risk Epithelial Dysplasia (HRED), low-risk Epithelial Dysplasia (LRED), and normal oral mucosa (NOM). The study consisted of 37 OSCC, 15 VC, 15 HRED, 15 LRED and 15 NOM. α-smooth muscle actin (α-SMA) antibody was used to identify myofibroblasts. The α-SMA expression was not observed in NOM and LRED. The α-SMA was expressed in 97.29% of OSCC, 86.66% of VC, 46.66 % of HRED. The α-SMA expression was significantly higher in OSCC than VC (p = 0.023) and HRED (p < 0.000). The α-SMA expression was significantly higher in VC than HRED (p = 0.043). Myofibroblastic expression, as highlighted by α-SMA, is undetectable in NOM and LRED but increases as the disease progresses from potentially malignant disorders, as HRED to VC to invasive OSCC. Thus, proliferation of myofibroblasts may be used as a stromal marker of oral premalignancy and malignancy.
Gaurav Vidhale - One of the best experts on this subject based on the ideXlab platform.
-
Comparison of Myofibroblasts Expression in Oral Squamous Cell Carcinoma, Verrucous Carcinoma, High Risk Epithelial Dysplasia, Low Risk Epithelial Dysplasia and Normal Oral Mucosa
Head and Neck Pathology, 2012Co-Authors: Minal Chaudhary, Amol Ramchandra Gadbail, Gaurav Vidhale, Mugdha P. Mankar (gadbail), Shailesh M. Gondivkar, Madhuri Gawande, Swati PatilAbstract:The aim was to evaluate and compare the presence of myofibroblasts in oral squamous cell carcinoma (OSCC), verrucous carcinoma (VC), high-risk Epithelial Dysplasia (HRED), low-risk Epithelial Dysplasia (LRED), and normal oral mucosa (NOM). The study consisted of 37 OSCC, 15 VC, 15 HRED, 15 LRED and 15 NOM. α-smooth muscle actin (α-SMA) antibody was used to identify myofibroblasts. The α-SMA expression was not observed in NOM and LRED. The α-SMA was expressed in 97.29% of OSCC, 86.66% of VC, 46.66 % of HRED. The α-SMA expression was significantly higher in OSCC than VC ( p = 0.023) and HRED ( p
-
comparison of myofibroblasts expression in oral squamous cell carcinoma verrucous carcinoma high risk Epithelial Dysplasia low risk Epithelial Dysplasia and normal oral mucosa
Head and Neck Pathology, 2012Co-Authors: Minal Chaudhary, Amol Ramchandra Gadbail, Gaurav Vidhale, Shailesh M. Gondivkar, Madhuri Gawande, Mugdha Mankar, Swati PatilAbstract:The aim was to evaluate and compare the presence of myofibroblasts in oral squamous cell carcinoma (OSCC), verrucous carcinoma (VC), high-risk Epithelial Dysplasia (HRED), low-risk Epithelial Dysplasia (LRED), and normal oral mucosa (NOM). The study consisted of 37 OSCC, 15 VC, 15 HRED, 15 LRED and 15 NOM. α-smooth muscle actin (α-SMA) antibody was used to identify myofibroblasts. The α-SMA expression was not observed in NOM and LRED. The α-SMA was expressed in 97.29% of OSCC, 86.66% of VC, 46.66 % of HRED. The α-SMA expression was significantly higher in OSCC than VC (p = 0.023) and HRED (p < 0.000). The α-SMA expression was significantly higher in VC than HRED (p = 0.043). Myofibroblastic expression, as highlighted by α-SMA, is undetectable in NOM and LRED but increases as the disease progresses from potentially malignant disorders, as HRED to VC to invasive OSCC. Thus, proliferation of myofibroblasts may be used as a stromal marker of oral premalignancy and malignancy.
Shailesh M. Gondivkar - One of the best experts on this subject based on the ideXlab platform.
-
Comparison of Myofibroblasts Expression in Oral Squamous Cell Carcinoma, Verrucous Carcinoma, High Risk Epithelial Dysplasia, Low Risk Epithelial Dysplasia and Normal Oral Mucosa
Head and Neck Pathology, 2012Co-Authors: Minal Chaudhary, Amol Ramchandra Gadbail, Gaurav Vidhale, Mugdha P. Mankar (gadbail), Shailesh M. Gondivkar, Madhuri Gawande, Swati PatilAbstract:The aim was to evaluate and compare the presence of myofibroblasts in oral squamous cell carcinoma (OSCC), verrucous carcinoma (VC), high-risk Epithelial Dysplasia (HRED), low-risk Epithelial Dysplasia (LRED), and normal oral mucosa (NOM). The study consisted of 37 OSCC, 15 VC, 15 HRED, 15 LRED and 15 NOM. α-smooth muscle actin (α-SMA) antibody was used to identify myofibroblasts. The α-SMA expression was not observed in NOM and LRED. The α-SMA was expressed in 97.29% of OSCC, 86.66% of VC, 46.66 % of HRED. The α-SMA expression was significantly higher in OSCC than VC ( p = 0.023) and HRED ( p
-
comparison of myofibroblasts expression in oral squamous cell carcinoma verrucous carcinoma high risk Epithelial Dysplasia low risk Epithelial Dysplasia and normal oral mucosa
Head and Neck Pathology, 2012Co-Authors: Minal Chaudhary, Amol Ramchandra Gadbail, Gaurav Vidhale, Shailesh M. Gondivkar, Madhuri Gawande, Mugdha Mankar, Swati PatilAbstract:The aim was to evaluate and compare the presence of myofibroblasts in oral squamous cell carcinoma (OSCC), verrucous carcinoma (VC), high-risk Epithelial Dysplasia (HRED), low-risk Epithelial Dysplasia (LRED), and normal oral mucosa (NOM). The study consisted of 37 OSCC, 15 VC, 15 HRED, 15 LRED and 15 NOM. α-smooth muscle actin (α-SMA) antibody was used to identify myofibroblasts. The α-SMA expression was not observed in NOM and LRED. The α-SMA was expressed in 97.29% of OSCC, 86.66% of VC, 46.66 % of HRED. The α-SMA expression was significantly higher in OSCC than VC (p = 0.023) and HRED (p < 0.000). The α-SMA expression was significantly higher in VC than HRED (p = 0.043). Myofibroblastic expression, as highlighted by α-SMA, is undetectable in NOM and LRED but increases as the disease progresses from potentially malignant disorders, as HRED to VC to invasive OSCC. Thus, proliferation of myofibroblasts may be used as a stromal marker of oral premalignancy and malignancy.