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Etienne Thiry - One of the best experts on this subject based on the ideXlab platform.

  • complete genome sequence of Equid Herpesvirus 3
    Genome Announcements, 2014
    Co-Authors: Steven Sijmons, A. Vissani, M.s. Tordoya, Etienne Thiry, Maria Barrandeguy, Benoit Muylkens, Piet Maes, Jelle Matthijnssens, Marc Van Ranst
    Abstract:

    ABSTRACT Equid Herpesvirus 3 (EHV-3) is a member of the subfamily Alphaherpesvirinae that causes equine coital exanthema. Here, we report the first complete genome sequence of EHV-3. The 151,601-nt genome encodes 76 distinct genes like other equine alphaHerpesviruses, but genetically, EHV-3 is significantly more divergent.

  • Experimental infection with Equid Herpesvirus 3 in seronegative and seropositive mares
    Veterinary Microbiology, 2012
    Co-Authors: Maria Barrandeguy, A. Vissani, Carlos Olguín, G. Barbara, H. Valenzuela, L. Becerra, M.s. Tordoya, Sayaka Mino, Etienne Thiry
    Abstract:

    Abstract Equine coital exanthema (ECE), caused by Equid Herpesvirus 3 (EHV-3), has been recognized as an economically significant venereal disease for years. However, no infection models on the natural host have been established. In order to set up an experimental infection protocol, seronegative and seropositive mares were topically inoculated in the perineal region with 4 × 10 6  TCID 50 /ml of EHV-3. Clinical signs were then evaluated by means of a designed scoring system, and body temperature was recorded daily. Virological, and serological studies were also performed. Typical ECE lesions, with clinical scores of 90, 92, 160 and 172, were observed in the four seronegative animals. Only mild ECE lesions were observed in the two seropositive mares, being the clinical scores 53 and 41. Both groups of mares shed the virus, but the duration of virus shedding was shorter and its intensity was lower in seropositive mares than in seronegative ones. Moreover, EHV-3 antibody response was detected in both seronegative and seropositive mares after experimental infection and re-infection, being more moderate in seropositive ones. As a conclusion, EHV-3 infection of mares was experimentally achieved in a reproducible manner. The typical lesions of ECE were observed after topical EHV-3 infection in seronegative mares, in association with virus excretion and neutralizing antibody kinetics.

  • Equine coital exanthema and its potential economic implications for the equine industry
    Veterinary journal (London England : 1997), 2011
    Co-Authors: M. Barrandeguy, Etienne Thiry
    Abstract:

    Abstract Equine coital exanthema (ECE) caused by Equid Herpesvirus 3 (EHV-3) is a contagious venereal disease characterised by the formation of painful papules, vesicles, pustules and ulcers on the external genitalia of both mares and stallions. EHV-3 is an alphaHerpesvirus that is distinct from the other equine Herpesviruses and endemic in most horse breeding populations worldwide. The negative impacts of ECE on equine breeding enterprises are the forced, temporary disruption of mating activities of mares and stallions, the additional care and supportive treatment that is required for affected horses, and the risk of virus spread by either fresh or frozen semen as well as by artificial insemination and embryo transfer. Because there are no effective surveillance systems to report ECE, its true prevalence and economic impact are difficult to assess and are probably underestimated. The purpose of this review is to describe the recent advances in understanding of EHV-3 infections and to consider the economic consequences of ECE within the current context of the equine industry.

  • subclinical infection and periodic shedding of Equid Herpesvirus 3
    Theriogenology, 2010
    Co-Authors: Maria Barrandeguy, A. Vissani, L. Becerra, Pont F Lezica, J Salamone, A Heguy, Olguin C Perglione, Etienne Thiry
    Abstract:

    The temporary disruption of reproductive activities due to equine coital exanthema (ECE), caused by Equid Herpesvirus 3 (EHV-3), at thoroughbred breeding facilities and embryo transfer centres, has an appreciable economic impact. The aim of the present study was to estimate the prevalence of excretion of EHV-3 in mares without clinical symptoms under field conditions and the re-excretion patterns of the virus in two seropositive (presumably latently infected) mares maintained in isolation for 11 mo. The EHV-3 virus was detected in perineal-vaginal swabs by real time PCR in 14 (6%) of 220 thoroughbred mares without clinical symptoms at the time of breeding. In the two isolated mares, re-excretion of EHV-3 was demonstrated on two occasions, 3 mo apart (each for a 3 d interval) in one mare, and on only 1 d in the other mare. Antibodies against EHV-3 were identified by seroneutralization in 105 (48%) of the thoroughbred mares, and during the entire period in the two isolated mares. Therefore, the present study provided evidence of EHV-3 shedders in a healthy mare population under both field and isolation conditions. Furthermore, at least two periods of spontaneous EHV-3 reactivation and re-excretion in the presence of serum antibodies occurred in one mare in an 11 mo interval. These findings could assist in the design and implementation of measures to minimize the spread of EHV-3 and control ECE outbreaks.

  • Occurrence of Equine Coital Exanthema in Mares from an Embryo Transfer Center
    Journal of Equine Veterinary Science, 2010
    Co-Authors: Maria Barrandeguy, A. Vissani, Julio Perkins, Jorge Mac Donough, Cecilia Olguin, Etienne Thiry
    Abstract:

    Abstract Equine coital exanthema (ECE) is an acute, venereal disease caused by Equid Herpesvirus 3 (EHV-3), characterized by the formation of papules, vesicles, pustules, and ulcers on the vaginal and vestibular mucosa as well as on the skin of the penis, prepuce, and perineal region of mares and stallions. The present work describes an outbreak of ECE characterized by typical lesions around the anus and on the perineal skin, discomfort, and anorectal lymphadenopathy, which occurred in a large number of donor and recipient mares at an embryo transfer center in Argentina. We found that the horses' movement pattern, the environmental conditions, and the management procedures could have favored the introduction and/or reactivation from latency and spread of EHV-3. Although this is the first report of an outbreak of ECE in an embryo transfer facility, it reinforces the need to introduce additional hygienic and preventive measures to avoid EHV-3 spread and future ECE outbreaks.

Maria Barrandeguy - One of the best experts on this subject based on the ideXlab platform.

  • complete genome sequence of Equid Herpesvirus 3
    Genome Announcements, 2014
    Co-Authors: Steven Sijmons, A. Vissani, M.s. Tordoya, Etienne Thiry, Maria Barrandeguy, Benoit Muylkens, Piet Maes, Jelle Matthijnssens, Marc Van Ranst
    Abstract:

    ABSTRACT Equid Herpesvirus 3 (EHV-3) is a member of the subfamily Alphaherpesvirinae that causes equine coital exanthema. Here, we report the first complete genome sequence of EHV-3. The 151,601-nt genome encodes 76 distinct genes like other equine alphaHerpesviruses, but genetically, EHV-3 is significantly more divergent.

  • Experimental infection with Equid Herpesvirus 3 in seronegative and seropositive mares
    Veterinary Microbiology, 2012
    Co-Authors: Maria Barrandeguy, A. Vissani, Carlos Olguín, G. Barbara, H. Valenzuela, L. Becerra, M.s. Tordoya, Sayaka Mino, Etienne Thiry
    Abstract:

    Abstract Equine coital exanthema (ECE), caused by Equid Herpesvirus 3 (EHV-3), has been recognized as an economically significant venereal disease for years. However, no infection models on the natural host have been established. In order to set up an experimental infection protocol, seronegative and seropositive mares were topically inoculated in the perineal region with 4 × 10 6  TCID 50 /ml of EHV-3. Clinical signs were then evaluated by means of a designed scoring system, and body temperature was recorded daily. Virological, and serological studies were also performed. Typical ECE lesions, with clinical scores of 90, 92, 160 and 172, were observed in the four seronegative animals. Only mild ECE lesions were observed in the two seropositive mares, being the clinical scores 53 and 41. Both groups of mares shed the virus, but the duration of virus shedding was shorter and its intensity was lower in seropositive mares than in seronegative ones. Moreover, EHV-3 antibody response was detected in both seronegative and seropositive mares after experimental infection and re-infection, being more moderate in seropositive ones. As a conclusion, EHV-3 infection of mares was experimentally achieved in a reproducible manner. The typical lesions of ECE were observed after topical EHV-3 infection in seronegative mares, in association with virus excretion and neutralizing antibody kinetics.

  • subclinical infection and periodic shedding of Equid Herpesvirus 3
    Theriogenology, 2010
    Co-Authors: Maria Barrandeguy, A. Vissani, L. Becerra, Pont F Lezica, J Salamone, A Heguy, Olguin C Perglione, Etienne Thiry
    Abstract:

    The temporary disruption of reproductive activities due to equine coital exanthema (ECE), caused by Equid Herpesvirus 3 (EHV-3), at thoroughbred breeding facilities and embryo transfer centres, has an appreciable economic impact. The aim of the present study was to estimate the prevalence of excretion of EHV-3 in mares without clinical symptoms under field conditions and the re-excretion patterns of the virus in two seropositive (presumably latently infected) mares maintained in isolation for 11 mo. The EHV-3 virus was detected in perineal-vaginal swabs by real time PCR in 14 (6%) of 220 thoroughbred mares without clinical symptoms at the time of breeding. In the two isolated mares, re-excretion of EHV-3 was demonstrated on two occasions, 3 mo apart (each for a 3 d interval) in one mare, and on only 1 d in the other mare. Antibodies against EHV-3 were identified by seroneutralization in 105 (48%) of the thoroughbred mares, and during the entire period in the two isolated mares. Therefore, the present study provided evidence of EHV-3 shedders in a healthy mare population under both field and isolation conditions. Furthermore, at least two periods of spontaneous EHV-3 reactivation and re-excretion in the presence of serum antibodies occurred in one mare in an 11 mo interval. These findings could assist in the design and implementation of measures to minimize the spread of EHV-3 and control ECE outbreaks.

  • Occurrence of Equine Coital Exanthema in Mares from an Embryo Transfer Center
    Journal of Equine Veterinary Science, 2010
    Co-Authors: Maria Barrandeguy, A. Vissani, Julio Perkins, Jorge Mac Donough, Cecilia Olguin, Etienne Thiry
    Abstract:

    Abstract Equine coital exanthema (ECE) is an acute, venereal disease caused by Equid Herpesvirus 3 (EHV-3), characterized by the formation of papules, vesicles, pustules, and ulcers on the vaginal and vestibular mucosa as well as on the skin of the penis, prepuce, and perineal region of mares and stallions. The present work describes an outbreak of ECE characterized by typical lesions around the anus and on the perineal skin, discomfort, and anorectal lymphadenopathy, which occurred in a large number of donor and recipient mares at an embryo transfer center in Argentina. We found that the horses' movement pattern, the environmental conditions, and the management procedures could have favored the introduction and/or reactivation from latency and spread of EHV-3. Although this is the first report of an outbreak of ECE in an embryo transfer facility, it reinforces the need to introduce additional hygienic and preventive measures to avoid EHV-3 spread and future ECE outbreaks.

A. Vissani - One of the best experts on this subject based on the ideXlab platform.

  • complete genome sequence of Equid Herpesvirus 3
    Genome Announcements, 2014
    Co-Authors: Steven Sijmons, A. Vissani, M.s. Tordoya, Etienne Thiry, Maria Barrandeguy, Benoit Muylkens, Piet Maes, Jelle Matthijnssens, Marc Van Ranst
    Abstract:

    ABSTRACT Equid Herpesvirus 3 (EHV-3) is a member of the subfamily Alphaherpesvirinae that causes equine coital exanthema. Here, we report the first complete genome sequence of EHV-3. The 151,601-nt genome encodes 76 distinct genes like other equine alphaHerpesviruses, but genetically, EHV-3 is significantly more divergent.

  • Experimental infection with Equid Herpesvirus 3 in seronegative and seropositive mares
    Veterinary Microbiology, 2012
    Co-Authors: Maria Barrandeguy, A. Vissani, Carlos Olguín, G. Barbara, H. Valenzuela, L. Becerra, M.s. Tordoya, Sayaka Mino, Etienne Thiry
    Abstract:

    Abstract Equine coital exanthema (ECE), caused by Equid Herpesvirus 3 (EHV-3), has been recognized as an economically significant venereal disease for years. However, no infection models on the natural host have been established. In order to set up an experimental infection protocol, seronegative and seropositive mares were topically inoculated in the perineal region with 4 × 10 6  TCID 50 /ml of EHV-3. Clinical signs were then evaluated by means of a designed scoring system, and body temperature was recorded daily. Virological, and serological studies were also performed. Typical ECE lesions, with clinical scores of 90, 92, 160 and 172, were observed in the four seronegative animals. Only mild ECE lesions were observed in the two seropositive mares, being the clinical scores 53 and 41. Both groups of mares shed the virus, but the duration of virus shedding was shorter and its intensity was lower in seropositive mares than in seronegative ones. Moreover, EHV-3 antibody response was detected in both seronegative and seropositive mares after experimental infection and re-infection, being more moderate in seropositive ones. As a conclusion, EHV-3 infection of mares was experimentally achieved in a reproducible manner. The typical lesions of ECE were observed after topical EHV-3 infection in seronegative mares, in association with virus excretion and neutralizing antibody kinetics.

  • subclinical infection and periodic shedding of Equid Herpesvirus 3
    Theriogenology, 2010
    Co-Authors: Maria Barrandeguy, A. Vissani, L. Becerra, Pont F Lezica, J Salamone, A Heguy, Olguin C Perglione, Etienne Thiry
    Abstract:

    The temporary disruption of reproductive activities due to equine coital exanthema (ECE), caused by Equid Herpesvirus 3 (EHV-3), at thoroughbred breeding facilities and embryo transfer centres, has an appreciable economic impact. The aim of the present study was to estimate the prevalence of excretion of EHV-3 in mares without clinical symptoms under field conditions and the re-excretion patterns of the virus in two seropositive (presumably latently infected) mares maintained in isolation for 11 mo. The EHV-3 virus was detected in perineal-vaginal swabs by real time PCR in 14 (6%) of 220 thoroughbred mares without clinical symptoms at the time of breeding. In the two isolated mares, re-excretion of EHV-3 was demonstrated on two occasions, 3 mo apart (each for a 3 d interval) in one mare, and on only 1 d in the other mare. Antibodies against EHV-3 were identified by seroneutralization in 105 (48%) of the thoroughbred mares, and during the entire period in the two isolated mares. Therefore, the present study provided evidence of EHV-3 shedders in a healthy mare population under both field and isolation conditions. Furthermore, at least two periods of spontaneous EHV-3 reactivation and re-excretion in the presence of serum antibodies occurred in one mare in an 11 mo interval. These findings could assist in the design and implementation of measures to minimize the spread of EHV-3 and control ECE outbreaks.

  • Occurrence of Equine Coital Exanthema in Mares from an Embryo Transfer Center
    Journal of Equine Veterinary Science, 2010
    Co-Authors: Maria Barrandeguy, A. Vissani, Julio Perkins, Jorge Mac Donough, Cecilia Olguin, Etienne Thiry
    Abstract:

    Abstract Equine coital exanthema (ECE) is an acute, venereal disease caused by Equid Herpesvirus 3 (EHV-3), characterized by the formation of papules, vesicles, pustules, and ulcers on the vaginal and vestibular mucosa as well as on the skin of the penis, prepuce, and perineal region of mares and stallions. The present work describes an outbreak of ECE characterized by typical lesions around the anus and on the perineal skin, discomfort, and anorectal lymphadenopathy, which occurred in a large number of donor and recipient mares at an embryo transfer center in Argentina. We found that the horses' movement pattern, the environmental conditions, and the management procedures could have favored the introduction and/or reactivation from latency and spread of EHV-3. Although this is the first report of an outbreak of ECE in an embryo transfer facility, it reinforces the need to introduce additional hygienic and preventive measures to avoid EHV-3 spread and future ECE outbreaks.

M.s. Tordoya - One of the best experts on this subject based on the ideXlab platform.

  • complete genome sequence of Equid Herpesvirus 3
    Genome Announcements, 2014
    Co-Authors: Steven Sijmons, A. Vissani, M.s. Tordoya, Etienne Thiry, Maria Barrandeguy, Benoit Muylkens, Piet Maes, Jelle Matthijnssens, Marc Van Ranst
    Abstract:

    ABSTRACT Equid Herpesvirus 3 (EHV-3) is a member of the subfamily Alphaherpesvirinae that causes equine coital exanthema. Here, we report the first complete genome sequence of EHV-3. The 151,601-nt genome encodes 76 distinct genes like other equine alphaHerpesviruses, but genetically, EHV-3 is significantly more divergent.

  • Experimental infection with Equid Herpesvirus 3 in seronegative and seropositive mares
    Veterinary Microbiology, 2012
    Co-Authors: Maria Barrandeguy, A. Vissani, Carlos Olguín, G. Barbara, H. Valenzuela, L. Becerra, M.s. Tordoya, Sayaka Mino, Etienne Thiry
    Abstract:

    Abstract Equine coital exanthema (ECE), caused by Equid Herpesvirus 3 (EHV-3), has been recognized as an economically significant venereal disease for years. However, no infection models on the natural host have been established. In order to set up an experimental infection protocol, seronegative and seropositive mares were topically inoculated in the perineal region with 4 × 10 6  TCID 50 /ml of EHV-3. Clinical signs were then evaluated by means of a designed scoring system, and body temperature was recorded daily. Virological, and serological studies were also performed. Typical ECE lesions, with clinical scores of 90, 92, 160 and 172, were observed in the four seronegative animals. Only mild ECE lesions were observed in the two seropositive mares, being the clinical scores 53 and 41. Both groups of mares shed the virus, but the duration of virus shedding was shorter and its intensity was lower in seropositive mares than in seronegative ones. Moreover, EHV-3 antibody response was detected in both seronegative and seropositive mares after experimental infection and re-infection, being more moderate in seropositive ones. As a conclusion, EHV-3 infection of mares was experimentally achieved in a reproducible manner. The typical lesions of ECE were observed after topical EHV-3 infection in seronegative mares, in association with virus excretion and neutralizing antibody kinetics.

Van Ranst Marc - One of the best experts on this subject based on the ideXlab platform.

  • Complete genome sequence of Equid Herpesvirus 3
    American Society for Microbiology, 2014
    Co-Authors: Sijmons Steven, Vissani Aldana, Tordoya, Maria Silva, Muylkens Benoît, Thiry Etienne, Maes Piet, Matthijnssens Jelle, Barrandeguy Maria, Van Ranst Marc
    Abstract:

    Equid Herpesvirus 3 (EHV-3) is a member of the subfamily Alphaherpesvirinae that causes equine coital exanthema. Here, we report the first complete genome sequence of EHV-3. The 151,601-nt genome encodes 76 distinct genes like other equine alphaHerpesviruses, but genetically, EHV-3 is significantly more divergent.status: publishe

  • Characterization of the Whole Genome of Equid Herpesvirus 3 by Next-Generation Sequencing
    2013
    Co-Authors: Sijmons Steven, Vissani Aldana, Muylkens Benoît, Thiry Etienne, Maes Piet, Matthijnssens Jelle, Barrandeguy Maria, Silva Tordoya Maria, Van Ranst Marc
    Abstract:

    Equid Herpesvirus 3 (EHV-3) is a member of the subfamily Alphaherpesvirinae that infects horses and causes equine coital exanthema. Although the whole genome of four equine alphaHerpesviruses (EHV-1, EHV-4, EHV-8 and EHV-9) has been published, the sequence knowledge for EHV-3 is limited to fragments of the DNA polymerase, the DNA packaging protein and the glycoprotein G. We therefore determined the complete genome sequence of EHV-3. EHV-3 was grown on equine dermal cells and purified with sucrose gradient ultracentrifugation. Subsequently, residual equine DNA was degraded through nuclease digestion after which viral DNA was extracted. Viral DNA was amplified using multiple displacement amplification and sequenced using 454 and Illumina next-generation sequencing platforms. Sequence reads were mapped on the equine genome and unmapped reads were collected for further assembly. De novo assemblies were conducted separately using 454 and Illumina datasets (with Newbler and Velvet respectively) and contigs were merged using Phrap. This resulted in 12 contigs that could be extended and joined by an iterative mapping of the sequence reads on the contig sequences. The resulting EHV-3 genome sequence is 152kb in size. EHV-3 ORFs are arranged in the same order as in the other known equine alphaHerpesviruses with no proof of additional ORFs. ORF76, however, is positioned inside the internal/terminal repeat and encodes only the carboxy-terminal half compared to other equine alphaHerpesviruses. Consequently, EHV-3 is predicted to encode 76 distinct genes, four of which are duplicated (ORFs 64, 65, 66 and 76) in the internal/terminal repeat, resulting in a total of 80 genes. Genetic comparison demonstrated that EHV-3 is the most divergent of the equine alphaHerpesviruses, possessing nucleotide similarities ranging from 62.4 to 65.4% with EHV-1, EHV-4, EHV-8 and EHV-9.status: publishe