The Experts below are selected from a list of 738 Experts worldwide ranked by ideXlab platform
Sadik Sogut - One of the best experts on this subject based on the ideXlab platform.
-
Protective agent, Erdosteine, against cisplatin-induced hepatic oxidant injury in rats.
Molecular and cellular biochemistry, 2020Co-Authors: Mehmet Duru, Harun Ciralik, Ramazan Akcan, Sadik SogutAbstract:Cisplatin, one of the most active cytotoxic agents against cancer, has several toxicities. Hepatotoxicity is one of them occurred during high doses treatment. The aim of this study was to determine the effects of Erdosteine against cisplatin-induced liver injury through tissue oxidant/antioxidant parameters and light microscopic evaluation. The rats were randomly divided into three groups: control (n=5), cisplatin (10 mg/kg, n=6) and cisplatin+Erdosteine (50 mg/kg/day oral Erdosteine, n=8) groups. The rats were sacrificed at the 5th day of cisplatin treatment. The liver tissues were examined with light microscopy and oxidant/antioxidant biochemical parameters. The malondialdehyde (MDA) and nitric oxide (NO) levels were increased in the cisplatin group in comparison with the control and cisplatin+Erdosteine groups (p
-
The protective role of Erdosteine on testicular tissue after testicular torsion and detorsion.
Molecular and cellular biochemistry, 2020Co-Authors: Adnan Narci, Mehmet Duru, Yesim Akaydin, H Serdar Gergerlioglu, Sadik SogutAbstract:Testicular torsion and detorsion are important clinical problems for infertile man and oxidative stress may have a role in this clinical situation. The aim of this study was to investigate the protective role of Erdosteine, an antioxidant, on unilateral testicular reperfusion injury in rats. The rats were divided into four groups including seven rats in each group: control, torsion, torsion/detorsion and torsion/detorsion+Erdosteine. Rats, except the sham operation group, were subjected to left unilateral torsion (720( composite function) rotation in the clockwise direction) without including the epididymis. The experiments were finished after sham operation time for control, 120 min torsion for torsion group and 120 min torsion and 240 min detorsion for torsion/detorsion groups. Bilateral orchiectomy was performed for all groups of rats. The ipsilateral and controlateral testis were divided into two pieces to analyse biochemical parameters and to investigate the light microscopic view. Malondialdehyde level of ipsilateral testis was increased in torsion and torsion/detorsion groups in comparison with the other groups (p < 0.05). Erdosteine treatment ameliorated lipid peroxidation after torsion/detorsion in ipsilateral testis (p < 0.05). Also, xanthine oxidase activity of ipsilateral testis was increased in torsion/detorsion group in comparison with the others (p < 0.05). Nitric oxide (NO) level of ipsilateral testis was higher in all experimental groups than sham operated control group (p < 0.05). Also, NO level of torsion group was increased in comparison with detorsion groups (p < 0.05). Erdosteine treatment caused increased glutathione peroxidase activity in comparison with torsion and torsion/detorsion groups and catalase activity in comparison with the other groups in ipsilateral testis (p < 0.05). Superoxide dismutase activity of ipsilateral testis was higher in torsion/detorsion and torsion/detorsion+Erdosteine groups than control and torsion groups (p < 0.05). The biochemical parameters were not affected in controlateral testis in all groups. Torsion, torsion/detorsion and torsion/detorsion+Erdosteine groups showed ipsilateral testicular damage in the histological examination, but the specimens from torsion/detorsion had a significantly greater histological injury than those from the other groups (p < 0.05). Control rats showed normal seminiferous tubule morphology. Rats in torsion group had slight-to-moderate disruption of the seminiferous epithelium. Rats in torsion/detorsion group displayed moderate-to-severe disruption of the seminiferous epithelium. In all animals from torsion/detorsion+Erdosteine group, the testicular tissues were affected with slight-to-moderate degenerative changes of the seminiferous epithelium. Administration of Erdosteine resulted in a significantly reduced histological damage associated with torsion of the spermatic cord compared with torsion/detorsion. In all groups, the contralateral testes were histologically normal. In conclusion, the results clearly displayed that Erdosteine treatment may have a protective role on testicular torsion/detorsion injury.
-
effects of Erdosteine on acetaminophen induced hepatotoxicity in rats
Toxicologic Pathology, 2008Co-Authors: Guven Kuvandik, Mehmet Duru, Ahmet Nacar, Zafer Yonden, Rami Helvaci, Tolunay Kozlu, Hasan Kaya, Sadik SogutAbstract:We investigated the effects of Erdosteine on acetaminophen (APAP)-induced hepatotoxicity in rats. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), AST (aspartate aminotransferase), and ALT (alanine transaminase) activities, and malonyldialdehyde (MDA) and nitric oxide levels as oxidant/antioxidant biochemical parameters were investigated with light microscopic evaluation in adult female Wistar Albino rats. APAP administration produced a decrease in hepatic SOD, CAT, and GSH-Px activities, and coadministration of Erdosteine (150 and 300 mg/kg) resulted in increases in the activities. MDA and NO levels increased in the APAP group, and Erdosteine treatments prevented these increases. Significant elevations in serum AST and ALT levels were observed in the APAP group, and when Erdosteine and APAP were coadministered, their serum levels were close to those in the control group. Light microscopic evaluation of livers showed that there were remarkable centrilobular (zone III) hepatic necrosis and mild to moderate sinusoidal congestion in the APAP group, whereas in the Erdosteine group, cellular necrosis was minimal and the hepatocytes maintained a better morphology when compared to the APAP group. Erdosteine prevented APAP-induced liver injury and toxic side effects probably through the antioxidant and radical scavenging effects of Erdosteine.
-
Erdosteine against acetaminophen induced renal toxicity
Molecular and Cellular Biochemistry, 2006Co-Authors: Bunyamin Isik, Ali Akcay, Reyhan Bayrak, Sadik SogutAbstract:Acetaminophen (APAP) induced toxicities have been a major problem in clinical practice. The aim of the present study was to demonstrate a possible protective role of Erdosteine, a mucolytic agent having antioxidant properties via its active metabolites, on APAP induced renal damage in rats. Female Wistar Albino rats were divided into groups including control, Erdosteine (150 mg/kg, oral), APAP (1 g/kg, oral) APAP+Erdosteine (150 mg/kg, oral) and APAP+Erdosteine (300 mg/kg, oral). APAP treatment caused lipid peroxidation as well as high NO level in renal tissue. Also, APAP treated rats had decreased activities of CAT and GSH-Px, but not SOD. In addition, tubular epithelial degeneration, vacuolization and cell desquamation were clearly observed in the APAP treated rats. The cellular debris in the proximal tubules and cortical interstitial congestions were prominent in the kidneys of APAP treated rats. BUN and creatinine levels were increased after APAP administration. All these pathological changes were reversed after Erdosteine treatments. Erdosteine treated APAP groups showed milder tubular degeneration, epithelial vacuolization in the proximal tubules, lesser cellular desquamation and better morphology when compared with APAP groups. In conclusion, Erdosteine may be a choice of preventive treatment against APAP induced nephrotoxicity.
-
the protective role of Erdosteine on testicular tissue after testicular torsion and detorsion
Molecular and Cellular Biochemistry, 2005Co-Authors: Adnan Narci, Mehmet Duru, Serdar H Gergerlioglu, Yesim Akaydin, Sadik SogutAbstract:Testicular torsion and detorsion are important clinical problems for infertile man and oxidative stress may have a role in this clinical situation. The aim of this study was to investigate the protective role of Erdosteine, an antioxidant, on unilateral testicular reperfusion injury in rats. The rats were divided into four groups including seven rats in each group: control, torsion, torsion/detorsion and torsion/detorsion+Erdosteine. Rats, except the sham operation group, were subjected to left unilateral torsion (720 ◦ rotation in the clockwise direction) without including the epididymis. The experiments were finished after sham operation time for control, 120 min torsion for torsion group and 120 min torsion and 240 min detorsion for torsion/detorsion groups. Bilateral orchiectomy was performed for all groups of rats. The ipsilateral and controlateral testis were divided into two pieces to analyse biochemical parameters and to investigate the light microscopic view. Malondialdehyde level of ipsilateral testis was increased in torsion and torsion/detorsion groups in comparison with the other groups ( p < 0.05). Erdosteine treatment ameliorated lipid peroxidation after torsion/detorsion in ipsilateral testis ( p < 0.05). Also, xanthine oxidase activity of ipsilateral testis was increased in torsion/detorsion group in comparison with the others ( p < 0.05). Nitric oxide (NO) level of ipsilateral testis was higher in all experimental groups than sham operated control group ( p < 0.05). Also, NO level of torsion group was increased in comparison with detorsion groups ( p < 0.05). Erdosteine treatment caused increased glutathione peroxidase activity in comparison with torsion and torsion/detorsion groups and catalase activity in comparison with the other groups in ipsilateral testis ( p < 0.05). Superoxide dismutase activity of ipsilateral testis was higher in torsion/detorsion and torsion/detorsion+Erdosteine groups than control and torsion groups ( p < 0.05). The biochemical parameters were not affected in controlateral testis in all groups. Torsion, torsion/detorsion and torsion/detorsion+Erdosteine groups showed ipsilateral testicular damage in the histological examination, but the specimens from torsion/detorsion had a significantly greater histological injury than those from the other groups ( p < 0.05). Control rats showed normal seminiferous tubule morphology. Rats in torsion group had slight-to-moderate disruption of the seminiferous epithelium. Rats in torsion/detorsion group displayed moderate-to-severe disruption of the seminiferous epithelium. In all animals from torsion/detorsion+Erdosteine group, the testicular tissues were affected with slight-to-moderate degenerative changes of the seminiferous epithelium. Administration of Erdosteine resulted in a significantly reduced histological damage associated with torsion of the spermatic cord compared with torsion/detorsion. In all groups, the contralateral testes were histologically normal. In conclusion, the results clearly displayed that Erdosteine treatment may have a protective role on testicular torsion/detorsion injury. (Mol Cell Biochem 280: 193‐199, 2005)
Omer Akyol - One of the best experts on this subject based on the ideXlab platform.
-
the protective effect of Erdosteine on short term global brain ischemia reperfusion injury in rats
Progress in Neuro-psychopharmacology & Biological Psychiatry, 2009Co-Authors: Elif Ozerol, Mustafa Iraz, Sedat Bilgic, Ahmet Cigli, Atilla Ilhan, Omer AkyolAbstract:Experimental studies have demonstrated that free radicals play a major role on neuronal injury during ischemia/reperfusion (I/R) in rats. Erdosteine is a thioderivative endowed with mucokinetic, mucolytic and free-radical-scavenging properties. The aim of the present study was to investigate the effect of Erdosteine treatment against short-term global brain ischemia/reperfusion injury in rats. The study was carried out on Wistar rats divided into four groups. (i) Control group, (ii) ischemia/reperfusion group, (iii) ischemia/reperfusion + Erdosteine group, and (iv) Erdosteine group. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities as well as thiobarbituric acid reactive substances (TBARSs) and nitric oxide (NO) levels were analysed in erythrocyte and plasma of rats. Plasma NO levels were significantly higher in the ischemia/reperfusion group than the other groups. The activities of SOD and GSH-Px were decreased, while TBARS levels increased in the ischemia/reperfusion group compared to other groups in both plasma and erythrocyte. The erythrocyte CAT activity was higher in Erdosteine group and there was a statistically significant increase, when compared with the Erdosteine plus ischemia/reperfusion group. By treating the rats with Erdosteine, the depletion of endogenous antioxidant enzymes (SOD, CAT, GSH-Px) and increase of TBARS and NO levels were prevented. This study, therefore, suggests that Erdosteine reduces parameters of oxidative stress is well supported by the data.
-
The protective effect of Erdosteine on short-term global brain ischemia/reperfusion injury in rats.
Progress in Neuro-psychopharmacology & Biological Psychiatry, 2008Co-Authors: Elif Ozerol, Mustafa Iraz, Sedat Bilgic, Ahmet Cigli, Atilla Ilhan, Omer AkyolAbstract:Experimental studies have demonstrated that free radicals play a major role on neuronal injury during ischemia/reperfusion (I/R) in rats. Erdosteine is a thioderivative endowed with mucokinetic, mucolytic and free-radical-scavenging properties. The aim of the present study was to investigate the effect of Erdosteine treatment against short-term global brain ischemia/reperfusion injury in rats. The study was carried out on Wistar rats divided into four groups. (i) Control group, (ii) ischemia/reperfusion group, (iii) ischemia/reperfusion + Erdosteine group, and (iv) Erdosteine group. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities as well as thiobarbituric acid reactive substances (TBARSs) and nitric oxide (NO) levels were analysed in erythrocyte and plasma of rats. Plasma NO levels were significantly higher in the ischemia/reperfusion group than the other groups. The activities of SOD and GSH-Px were decreased, while TBARS levels increased in the ischemia/reperfusion group compared to other groups in both plasma and erythrocyte. The erythrocyte CAT activity was higher in Erdosteine group and there was a statistically significant increase, when compared with the Erdosteine plus ischemia/reperfusion group. By treating the rats with Erdosteine, the depletion of endogenous antioxidant enzymes (SOD, CAT, GSH-Px) and increase of TBARS and NO levels were prevented. This study, therefore, suggests that Erdosteine reduces parameters of oxidative stress is well supported by the data.
-
Erdosteine ameliorates neurological outcome and oxidative stress due to ischemia reperfusion injury in rabbit spinal cord
European Journal of Vascular and Endovascular Surgery, 2004Co-Authors: Atilla Ilhan, Omer Akyol, Ahmet Gurel, Suleyman OzenAbstract:OBJECTIVE: Oxygen-derived free radicals have been suggested as important in degeneration after spinal cord ischemia. The aim of this study was to investigate whether Erdosteine has a protective effect against spinal cord ischemia during aortic cross clamping. MATERIALS AND METHODS: New Zealand White rabbits (n=21) were divided into three groups. In the ischemia/reperfusion group (I/R) (n=8), the infrarenal aorta of rabbits was cross clamped for 21 min and then reperfused. In Erdosteine group, the administration of Erdosteine solution (50 mg/kg) was started two days before aortic cross-clamping and rabbits (n=8) were subjected to ischemia and reperfusion. Animals in control group (n=5) underwent a surgical procedure similar to the other groups but the aorta was not clamped. The animals were sacrificed at 72 h and histopathological, and biochemical analyses were carried out on the lumbar spinal cords. RESULTS: Erdosteine treatment was associated with improved neurological function in the postoperative period. Histopathological examination of spinal cord tissues in Erdosteine group revealed changes consistent with mild ischemic injury, but rabbits in I/R group with paraplegia had total destruction of the motor neurons. Biochemical analyses of spinal cord tissues, in the I/R group, revealed a significant increase in the superoxide dismutase, xanthine oxidase, adenosine deaminase and myeloperoxidase activities, and a significant depletion in glutathione peroxidase activity when compared to that of control rabbits. Erdosteine treatment prevented the increase of all these enzymes except adenosine deaminase. Ischemia/reperfusion produced a significant increase in the tissue malondialdehyde levels. Ischemia/reperfusion-induced increments in malondialdehyde content of the spinal cord were significantly prevented by Erdosteine treatment. CONCLUSIONS: The present study demonstrated that Erdosteine treatment before aortic cross clamping ameliorates neurological outcome, neuronal injury and oxidative stress in the rabbit spinal cord.
-
the effects of Erdosteine on the activities of some metabolic enzymes during cisplatin induced nephrotoxicity in rats
Pharmacological Research, 2004Co-Authors: Ramazan H Yilmaz, Sadik Sogut, Mustafa Iraz, Huseyin Ozyurt, Zeki Yildirim, Omer Akyol, Serdar GergerliogluAbstract:Abstract Cisplatin is one of the widely used chemothrapeutic agents. One of the major side effects of the drug is renal toxicity. The aims of the presented study was (1) to investigate the effect of cisplatin on some renal metabolic enzyme activities such as hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD), lactate dehydrogenase (LDH), and malate dehydrogenase (MDH) in an experimental model of acute renal failure and (2) to examine the protective role of Erdosteine, an expectorant agent which has also antioxidant properties on cisplatin–induced nephrotoxicity and the enzyme activities mentioned above. Female Wistar albino rats were divided into three groups: sham operation group (n=6), cisplatin group (n=9), erdostein+cisplatin group (n=8). All the chemicals used were applied intraperitoneally. Hexokinase, G6PD, LDH, and MDH activities were determined in the kidney supernatant at the end of the surgical procedures. Spectrophotometric methods were used to determine the activities of above-mentioned enzymes in the kidney tissue. Hexokinase and G6PD activities were found to be increased in cisplatin group compared to control group. G6PD activities were found to be decreased in Erdosteine+cisplatin group compared to cisplatin group. There were minimal changes in LDH and MDH activities of the two study groups compared with the control group. The results obtained suggested that the glucose metabolizing metabolic pathways of renal tissue were partially affected from cisplatin toxicity and Erdosteine have some protective effects on these enzyme activities.
-
effects of aminoguanidine and antioxidant Erdosteine on bleomycin induced lung fibrosis in rats
Nitric Oxide, 2004Co-Authors: Zeki Yildirim, Mustafa Iraz, Ahmet Gurel, Yusuf Turkoz, Mahir Kotuk, Ferah Armutcu, Suleyman Ozen, Ismet Aydogdu, Omer AkyolAbstract:Abstract Reactive oxygen and nitrogen species have been implicated in the pathogenesis of bleomycin-induced lung fibrosis. The effects of aminoguanidine and Erdosteine on the bleomycin-induced lung fibrosis were evaluated in rats. The animals were placed into five groups: Vehicle + vehicle, vehicle + bleomycin (2.5 U/kg), bleomycin + aminoguanidine (200 mg/kg), bleomycin + Erdosteine (10 mg/kg), and bleomycin + Erdosteine + aminoguanidine. Bleomycin administration resulted in prominent lung fibrosis as measured by lung hydroxyproline content and lung histology, which is completely prevented by Erdosteine and aminoguanidine. A strong staining for nitro tyrosine antibody in lung tissue and increased levels of lung NO were found in bleomycin group, that were significantly reduced by aminoguanidine and Erdosteine. Aminoguanidine and Erdosteine significantly prevented depletion of superoxide dismutase and glutathione peroxidase and elevated myeloperoxidase activities, malondialdehyde level in lung tissue produced by bleomycin. Data presented here indicate that aminoguanidine and Erdosteine prevented bleomycin-induced lung fibrosis and that nitric oxide mediated tyrosine nitration of proteins plays a significant role in the pathogenesis of bleomycin-induced lung fibrosis. Also our data suggest that antifibrotic affect of antioxidants may be due to their inhibitory effect on nitric oxide generation in this model.
Mustafa Iraz - One of the best experts on this subject based on the ideXlab platform.
-
the protective effect of Erdosteine on short term global brain ischemia reperfusion injury in rats
Progress in Neuro-psychopharmacology & Biological Psychiatry, 2009Co-Authors: Elif Ozerol, Mustafa Iraz, Sedat Bilgic, Ahmet Cigli, Atilla Ilhan, Omer AkyolAbstract:Experimental studies have demonstrated that free radicals play a major role on neuronal injury during ischemia/reperfusion (I/R) in rats. Erdosteine is a thioderivative endowed with mucokinetic, mucolytic and free-radical-scavenging properties. The aim of the present study was to investigate the effect of Erdosteine treatment against short-term global brain ischemia/reperfusion injury in rats. The study was carried out on Wistar rats divided into four groups. (i) Control group, (ii) ischemia/reperfusion group, (iii) ischemia/reperfusion + Erdosteine group, and (iv) Erdosteine group. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities as well as thiobarbituric acid reactive substances (TBARSs) and nitric oxide (NO) levels were analysed in erythrocyte and plasma of rats. Plasma NO levels were significantly higher in the ischemia/reperfusion group than the other groups. The activities of SOD and GSH-Px were decreased, while TBARS levels increased in the ischemia/reperfusion group compared to other groups in both plasma and erythrocyte. The erythrocyte CAT activity was higher in Erdosteine group and there was a statistically significant increase, when compared with the Erdosteine plus ischemia/reperfusion group. By treating the rats with Erdosteine, the depletion of endogenous antioxidant enzymes (SOD, CAT, GSH-Px) and increase of TBARS and NO levels were prevented. This study, therefore, suggests that Erdosteine reduces parameters of oxidative stress is well supported by the data.
-
The protective effect of Erdosteine on short-term global brain ischemia/reperfusion injury in rats.
Progress in Neuro-psychopharmacology & Biological Psychiatry, 2008Co-Authors: Elif Ozerol, Mustafa Iraz, Sedat Bilgic, Ahmet Cigli, Atilla Ilhan, Omer AkyolAbstract:Experimental studies have demonstrated that free radicals play a major role on neuronal injury during ischemia/reperfusion (I/R) in rats. Erdosteine is a thioderivative endowed with mucokinetic, mucolytic and free-radical-scavenging properties. The aim of the present study was to investigate the effect of Erdosteine treatment against short-term global brain ischemia/reperfusion injury in rats. The study was carried out on Wistar rats divided into four groups. (i) Control group, (ii) ischemia/reperfusion group, (iii) ischemia/reperfusion + Erdosteine group, and (iv) Erdosteine group. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities as well as thiobarbituric acid reactive substances (TBARSs) and nitric oxide (NO) levels were analysed in erythrocyte and plasma of rats. Plasma NO levels were significantly higher in the ischemia/reperfusion group than the other groups. The activities of SOD and GSH-Px were decreased, while TBARS levels increased in the ischemia/reperfusion group compared to other groups in both plasma and erythrocyte. The erythrocyte CAT activity was higher in Erdosteine group and there was a statistically significant increase, when compared with the Erdosteine plus ischemia/reperfusion group. By treating the rats with Erdosteine, the depletion of endogenous antioxidant enzymes (SOD, CAT, GSH-Px) and increase of TBARS and NO levels were prevented. This study, therefore, suggests that Erdosteine reduces parameters of oxidative stress is well supported by the data.
-
attenuation of bleomycin induced lung fibrosis by oral sulfhydryl containing antioxidants in rats Erdosteine and n acetylcysteine
Pulmonary Pharmacology & Therapeutics, 2005Co-Authors: Zeki Yildirim, Mustafa Iraz, Irfan Kuku, Mahir Kotuk, Ferah Armutcu, Suleyman OzenAbstract:: Antioxidant therapy may be useful in diseases with impaired oxidant antioxidant balance such as lung fibrosis. The effects of sulfhydryl-containing antioxidant agents N-acetylcysteine (NAC) and Erdosteine on the bleomycin-induced lung fibrosis were compared in rats. The animals were divided into four groups: Vehicle + vehicle, vehicle + bleomycin (2.5 U/kg), bleomycin + (10 mg/kg), and bleomycin + NAC (3 mmol/kg). Bleomycin administration resulted in prominent lung fibrosis as measured by lung hydroxyproline content and lung histology which is almost completely prevented by Erdosteine and NAC. Hydroxyproline content was 18.7 +/- 3.5 and 11.2 +/- 0.6 mg/g dried tissue in bleomycin and saline treated rats, respectively (P < 0.001), and this level was 11.3 +/- 1.2 and 13.8 +/- 1.2 mg/g dried tissue in Erdosteine and NAC pretreated, respectively. Erdosteine and NAC significantly reduced depletion of glutathione peroxidase, and prevented increases in myeloperoxidase activities, nitric oxide, and malondialdehyde levels in lung tissue produced by bleomycin. Data presented here indicate that Erdosteine and NAC similarly prevented bleomycin-induced lung fibrosis and their antioxidant effects were also similar in this experiment.
-
the effects of Erdosteine on the activities of some metabolic enzymes during cisplatin induced nephrotoxicity in rats
Pharmacological Research, 2004Co-Authors: Ramazan H Yilmaz, Sadik Sogut, Mustafa Iraz, Huseyin Ozyurt, Zeki Yildirim, Omer Akyol, Serdar GergerliogluAbstract:Abstract Cisplatin is one of the widely used chemothrapeutic agents. One of the major side effects of the drug is renal toxicity. The aims of the presented study was (1) to investigate the effect of cisplatin on some renal metabolic enzyme activities such as hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD), lactate dehydrogenase (LDH), and malate dehydrogenase (MDH) in an experimental model of acute renal failure and (2) to examine the protective role of Erdosteine, an expectorant agent which has also antioxidant properties on cisplatin–induced nephrotoxicity and the enzyme activities mentioned above. Female Wistar albino rats were divided into three groups: sham operation group (n=6), cisplatin group (n=9), erdostein+cisplatin group (n=8). All the chemicals used were applied intraperitoneally. Hexokinase, G6PD, LDH, and MDH activities were determined in the kidney supernatant at the end of the surgical procedures. Spectrophotometric methods were used to determine the activities of above-mentioned enzymes in the kidney tissue. Hexokinase and G6PD activities were found to be increased in cisplatin group compared to control group. G6PD activities were found to be decreased in Erdosteine+cisplatin group compared to cisplatin group. There were minimal changes in LDH and MDH activities of the two study groups compared with the control group. The results obtained suggested that the glucose metabolizing metabolic pathways of renal tissue were partially affected from cisplatin toxicity and Erdosteine have some protective effects on these enzyme activities.
-
effects of aminoguanidine and antioxidant Erdosteine on bleomycin induced lung fibrosis in rats
Nitric Oxide, 2004Co-Authors: Zeki Yildirim, Mustafa Iraz, Ahmet Gurel, Yusuf Turkoz, Mahir Kotuk, Ferah Armutcu, Suleyman Ozen, Ismet Aydogdu, Omer AkyolAbstract:Abstract Reactive oxygen and nitrogen species have been implicated in the pathogenesis of bleomycin-induced lung fibrosis. The effects of aminoguanidine and Erdosteine on the bleomycin-induced lung fibrosis were evaluated in rats. The animals were placed into five groups: Vehicle + vehicle, vehicle + bleomycin (2.5 U/kg), bleomycin + aminoguanidine (200 mg/kg), bleomycin + Erdosteine (10 mg/kg), and bleomycin + Erdosteine + aminoguanidine. Bleomycin administration resulted in prominent lung fibrosis as measured by lung hydroxyproline content and lung histology, which is completely prevented by Erdosteine and aminoguanidine. A strong staining for nitro tyrosine antibody in lung tissue and increased levels of lung NO were found in bleomycin group, that were significantly reduced by aminoguanidine and Erdosteine. Aminoguanidine and Erdosteine significantly prevented depletion of superoxide dismutase and glutathione peroxidase and elevated myeloperoxidase activities, malondialdehyde level in lung tissue produced by bleomycin. Data presented here indicate that aminoguanidine and Erdosteine prevented bleomycin-induced lung fibrosis and that nitric oxide mediated tyrosine nitration of proteins plays a significant role in the pathogenesis of bleomycin-induced lung fibrosis. Also our data suggest that antifibrotic affect of antioxidants may be due to their inhibitory effect on nitric oxide generation in this model.
Ramazan H Yilmaz - One of the best experts on this subject based on the ideXlab platform.
-
Erdosteine modulates radiocontrast induced hepatotoxicity in rat
Cell Biochemistry and Function, 2009Co-Authors: Ahmet Yesildag, Ramazan H Yilmaz, Efkan Uz, Ahmet Ozden, Yetkin Agackiran, Mihrican Yesildag, Nigar Yilmaz, Rana Sirmali, Huseyin Vural, Mustafa NazirogluAbstract:It has been suggested that reactive oxygen species (ROS) plays an important role in radio contrast media (RCM)-induced ischemia reperfusion tissue injury although antioxidants may have protective effects on the injury. We investigated the effects of Erdosteine as an antioxidant agent on RCM-induced liver toxicity in rats by evaluation of lipid peroxidation (as TBARS), catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH) and glutathione peroxidase (GSH-Px) values and histological evaluation. Twenty-one rats were equally divided into three groups as follows: control, RCM, and RCM plus Erdosteine. RCM was intraperitoneally administered for 1 day. Erdosteine was administered orally for 2 days after RCM administration. Liver samples were taken from the rats and they homogenized in a motor-driven tissue homogenizer. TBARS levels were significantly (p < 0.005) higher in RCM group than in control although SOD activities significantly (p < 0.05) decreased in RCM group. TBARS levels were lower in RCM plus Erdosteine group than in control although SOD activity and GSH level increased (p < 0.05) in liver as compared to RCM alone. Erdosteine showed also histopathological protection (p < 0.0001) against RCM induced hepatotoxicity. GSH-Px and CAT activities were not statistically changed by the Erdosteine. According to our results, it can be concluded that radiocontrast media can induce oxidative stress in liver as suggested by previous studies. Erdosteine seems to be protective agent on the radiocontrast media-induced liver toxicity by inhibiting the production of ROS via the enzymatic antioxidant system. Copyright © 2009 John Wiley & Sons, Ltd.
-
protective effects of Erdosteine and vitamins c and e combination on ischemia reperfusion induced lung oxidative stress and plasma copper and zinc levels in a rat hind limb model
Biological Trace Element Research, 2007Co-Authors: Mehmet Sırmalı, Ramazan H Yilmaz, Efkan Uz, Rana Sirmali, Mustafa Naziroglu, Aynur Kılbaş, İrfan Altuntaş, Namık Delibaş, Huseyin VuralAbstract:The aim of this study was to investigate the protective effects of Erdosteine and vitamins C and E (VCE) on the lungs after performing hind limb ischemia–reperfusion (I/R) by assessing oxidative stress, plasma copper (Cu), and zinc (Zn) analysis. The animals were divided randomly into four groups as nine rats each as follows: control, I/R, I/R plus Erdosteine, and I/R plus VCE combination. I/R period for 60 min was performed on the both hind limbs of all the rats in the groups of I/R, Erdosteine with I/R, VCE with I/R allowing 120 min of reperfusion. The animals received orally Erdosteine one time in a day and 3 days before I/R in the Erdosteine group. In the VCE group, the animals VCE combination received one time in a day and 3 days before I/R, although placebo was given to control and I/R group animals. Lung lipid peroxidation (malondialdehyde [MDA]) level, superoxide dismutase (SOD), and catalase activities were increased, although lung glutathione (GSH) and plasma Zn levels decreased in I/R group in lung tissue compared with the control group. Serum MDA level, creatine kinase, and lactate dehydrogenase activities were increased in I/R group compared with the control. Lung MDA and plasma Zn levels and lung SOD activity were decreased by Erdosteine administration, whereas lung GSH levels after I/R increased. The plasma Zn levels and lung SOD activity were decreased by VCE administration, although the plasma Cu and lung GSH levels increased after I/R. In conclusion, Erdosteine has an antioxidant role on the values in the rat model, and it has more protective affect than in VCE in attenuating I/R-induced lung injury in rats.
-
protective role of Erdosteine on vancomycin induced oxidative stress in rat liver
Molecular and Cellular Biochemistry, 2006Co-Authors: Mehmet Sahin, Efkan Uz, Seref Olgar, Sevket Ercan Tunc, Cagatay Arslan, Ramazan H YilmazAbstract:Drug-induced liver toxicity is a common cause of liver injury. This study was designed to elucidate whether high dose vancomycin (VCM) induces oxidative stress in liver and to investigate the protective effects of Erdosteine, an expectorant agent. Twenty-two young Wistar rats were divided into three groups as follows: control group, VCM, and VCM plus Erdosteine. VCM was administered intraperitoneally in the dosage of 200 mg/kg twice daily for 7 days. Erdosteine was administered orally administered once a day at a dose of 10 mg/kg body weight. The activities of antioxidant enzymes such as superoxide dismutase and catalase as well as the concentration of malondialdehyde, as an indicator of lipid peroxidation, were measured to evaluate oxidative stress in homogenates of the liver. VCM administration increased malondialdehyde levels (p < 0.001), superoxide dismutase (p < 0.01) and catalase (p < 0.001) activities. Erdosteine co-administration with VCM injections caused significantly decreased malondialdehyde levels (p < 0.001), superoxide dismutase (p < 0.01) and catalase (p < 0.001) activities in liver tissue when compared with VCM alone. It can be concluded that Erdosteine may prevent VCM-induced oxidative changes in liver by reducing reactive oxygen species.
-
in vivo evidences suggesting the role of oxidative stress in pathogenesis of vancomycin induced nephrotoxicity protection by Erdosteine
Toxicology, 2005Co-Authors: Faruk Oktem, Ramazan H Yilmaz, Meltem Koyuncu Arslan, Fehmi Ozguner, Ozden Candir, Metin Ciris, Efkan UzAbstract:Abstract The aims of this study were to examine vancomycin (VCM)-induced oxidative stress that promotes production of reactive oxygen species (ROS) and to investigate the role of Erdosteine, an expectorant agent, which has also antioxidant properties, on kidney tissue against the possible VCM-induced renal impairment in rats. Rats were divided into three groups: sham, VCM and VCM plus Erdosteine. VCM was administrated intraperitoneally (i.p.) with 200 mg kg −1 twice daily for 7 days. Erdosteine was administered orally. VCM administration to control rats significantly increased renal malondialdehyde (MDA) and urinary N -acetyl-β- d -glucosaminidase (NAG, a marker of renal tubular injury) excretion but decreased superoxide dismutase (SOD) and catalase (CAT) activities. Erdosteine administration with VCM injections caused significantly decreased renal MDA and urinary NAG excretion, and increased SOD activity, but not CAT activity in renal tissue when compared with VCM alone. Erdosteine showed histopathological protection against VCM-induced nephrotoxicity. There were a significant dilatation of tubular lumens, extensive epithelial cell vacuolization, atrophy, desquamation, and necrosis in VCM-treated rats more than those of the control and the Erdosteine groups. Erdosteine caused a marked reduction in the extent of tubular damage. It is concluded that oxidative tubular damage plays an important role in the VCM-induced nephrotoxicity and the modulation of oxidative stress with Erdosteine reduces the VCM-induced kidney damage both at the biochemical and histological levels.
-
the effects of Erdosteine on the activities of some metabolic enzymes during cisplatin induced nephrotoxicity in rats
Pharmacological Research, 2004Co-Authors: Ramazan H Yilmaz, Sadik Sogut, Mustafa Iraz, Huseyin Ozyurt, Zeki Yildirim, Omer Akyol, Serdar GergerliogluAbstract:Abstract Cisplatin is one of the widely used chemothrapeutic agents. One of the major side effects of the drug is renal toxicity. The aims of the presented study was (1) to investigate the effect of cisplatin on some renal metabolic enzyme activities such as hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD), lactate dehydrogenase (LDH), and malate dehydrogenase (MDH) in an experimental model of acute renal failure and (2) to examine the protective role of Erdosteine, an expectorant agent which has also antioxidant properties on cisplatin–induced nephrotoxicity and the enzyme activities mentioned above. Female Wistar albino rats were divided into three groups: sham operation group (n=6), cisplatin group (n=9), erdostein+cisplatin group (n=8). All the chemicals used were applied intraperitoneally. Hexokinase, G6PD, LDH, and MDH activities were determined in the kidney supernatant at the end of the surgical procedures. Spectrophotometric methods were used to determine the activities of above-mentioned enzymes in the kidney tissue. Hexokinase and G6PD activities were found to be increased in cisplatin group compared to control group. G6PD activities were found to be decreased in Erdosteine+cisplatin group compared to cisplatin group. There were minimal changes in LDH and MDH activities of the two study groups compared with the control group. The results obtained suggested that the glucose metabolizing metabolic pathways of renal tissue were partially affected from cisplatin toxicity and Erdosteine have some protective effects on these enzyme activities.
Zeki Yildirim - One of the best experts on this subject based on the ideXlab platform.
-
attenuation of bleomycin induced lung fibrosis by oral sulfhydryl containing antioxidants in rats Erdosteine and n acetylcysteine
Pulmonary Pharmacology & Therapeutics, 2005Co-Authors: Zeki Yildirim, Mustafa Iraz, Irfan Kuku, Mahir Kotuk, Ferah Armutcu, Suleyman OzenAbstract:: Antioxidant therapy may be useful in diseases with impaired oxidant antioxidant balance such as lung fibrosis. The effects of sulfhydryl-containing antioxidant agents N-acetylcysteine (NAC) and Erdosteine on the bleomycin-induced lung fibrosis were compared in rats. The animals were divided into four groups: Vehicle + vehicle, vehicle + bleomycin (2.5 U/kg), bleomycin + (10 mg/kg), and bleomycin + NAC (3 mmol/kg). Bleomycin administration resulted in prominent lung fibrosis as measured by lung hydroxyproline content and lung histology which is almost completely prevented by Erdosteine and NAC. Hydroxyproline content was 18.7 +/- 3.5 and 11.2 +/- 0.6 mg/g dried tissue in bleomycin and saline treated rats, respectively (P < 0.001), and this level was 11.3 +/- 1.2 and 13.8 +/- 1.2 mg/g dried tissue in Erdosteine and NAC pretreated, respectively. Erdosteine and NAC significantly reduced depletion of glutathione peroxidase, and prevented increases in myeloperoxidase activities, nitric oxide, and malondialdehyde levels in lung tissue produced by bleomycin. Data presented here indicate that Erdosteine and NAC similarly prevented bleomycin-induced lung fibrosis and their antioxidant effects were also similar in this experiment.
-
the effects of Erdosteine on the activities of some metabolic enzymes during cisplatin induced nephrotoxicity in rats
Pharmacological Research, 2004Co-Authors: Ramazan H Yilmaz, Sadik Sogut, Mustafa Iraz, Huseyin Ozyurt, Zeki Yildirim, Omer Akyol, Serdar GergerliogluAbstract:Abstract Cisplatin is one of the widely used chemothrapeutic agents. One of the major side effects of the drug is renal toxicity. The aims of the presented study was (1) to investigate the effect of cisplatin on some renal metabolic enzyme activities such as hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD), lactate dehydrogenase (LDH), and malate dehydrogenase (MDH) in an experimental model of acute renal failure and (2) to examine the protective role of Erdosteine, an expectorant agent which has also antioxidant properties on cisplatin–induced nephrotoxicity and the enzyme activities mentioned above. Female Wistar albino rats were divided into three groups: sham operation group (n=6), cisplatin group (n=9), erdostein+cisplatin group (n=8). All the chemicals used were applied intraperitoneally. Hexokinase, G6PD, LDH, and MDH activities were determined in the kidney supernatant at the end of the surgical procedures. Spectrophotometric methods were used to determine the activities of above-mentioned enzymes in the kidney tissue. Hexokinase and G6PD activities were found to be increased in cisplatin group compared to control group. G6PD activities were found to be decreased in Erdosteine+cisplatin group compared to cisplatin group. There were minimal changes in LDH and MDH activities of the two study groups compared with the control group. The results obtained suggested that the glucose metabolizing metabolic pathways of renal tissue were partially affected from cisplatin toxicity and Erdosteine have some protective effects on these enzyme activities.
-
effects of aminoguanidine and antioxidant Erdosteine on bleomycin induced lung fibrosis in rats
Nitric Oxide, 2004Co-Authors: Zeki Yildirim, Mustafa Iraz, Ahmet Gurel, Yusuf Turkoz, Mahir Kotuk, Ferah Armutcu, Suleyman Ozen, Ismet Aydogdu, Omer AkyolAbstract:Abstract Reactive oxygen and nitrogen species have been implicated in the pathogenesis of bleomycin-induced lung fibrosis. The effects of aminoguanidine and Erdosteine on the bleomycin-induced lung fibrosis were evaluated in rats. The animals were placed into five groups: Vehicle + vehicle, vehicle + bleomycin (2.5 U/kg), bleomycin + aminoguanidine (200 mg/kg), bleomycin + Erdosteine (10 mg/kg), and bleomycin + Erdosteine + aminoguanidine. Bleomycin administration resulted in prominent lung fibrosis as measured by lung hydroxyproline content and lung histology, which is completely prevented by Erdosteine and aminoguanidine. A strong staining for nitro tyrosine antibody in lung tissue and increased levels of lung NO were found in bleomycin group, that were significantly reduced by aminoguanidine and Erdosteine. Aminoguanidine and Erdosteine significantly prevented depletion of superoxide dismutase and glutathione peroxidase and elevated myeloperoxidase activities, malondialdehyde level in lung tissue produced by bleomycin. Data presented here indicate that aminoguanidine and Erdosteine prevented bleomycin-induced lung fibrosis and that nitric oxide mediated tyrosine nitration of proteins plays a significant role in the pathogenesis of bleomycin-induced lung fibrosis. Also our data suggest that antifibrotic affect of antioxidants may be due to their inhibitory effect on nitric oxide generation in this model.
-
cisplatin induced acute renal failure is ameliorated by Erdosteine in a dose dependent manner
Journal of Applied Toxicology, 2004Co-Authors: Huseyin Ozyurt, Ramazan H Yilmaz, Mustafa Iraz, Zeki Yildirim, Mahir Kotuk, Murat Yagmurca, Sad Sogut, Serdar GergerliogluAbstract:The aim of this study was to investigate the optimum dosage of Erdosteine to ameliorate cisplatin-induced nephrotoxicity. Three different doses of Erdosteine at 25, 50 and 75 mg kg−1 were studied in rats. Intraperitoneal administration of 7 mg kg−1 cisplatin led to acute renal failure, as indicated by kidney histology and increases in plasma creatinine and blood urea nitrogen (BUN) levels. At 5 days after cisplatin injection the BUN level was increased significantly from 15.1 ± 4.3 to 126.7 ± 152.6 mg dl−1 and plasma creatinine levels increased from 0.37 ± 0.005 to 1.68 ± 1.9 mg dl−1. When the rats were administered 50 and 75 mg kg−1 Erdosteine 24 h before cisplatin injection that was continued until sacrifice (total of 6 days), the BUN and creatinine levels remained similar to control levels and the grade of histology was similar. Erdosteine at doses of 50 and 75 mg kg−1 ameliorates cisplatin-induced renal failure. The optimum dose of Erdosteine may be 50 mg kg−1 in this study. Copyright © 2004 John Wiley & Sons, Ltd.
-
Erdosteine prevents bleomycin induced pulmonary fibrosis in rats
European Journal of Pharmacology, 2004Co-Authors: Sadik Sogut, Mustafa Iraz, Huseyin Ozyurt, Omer Akyol, Ferah Armutcu, Suleyman Ozen, Levent Kart, Suleyman Kaplan, Ismail Temel, Zeki YildirimAbstract:Oxidative stress plays an important role in the pathogenesis of idiopathic pulmonary fibrosis. Therefore, Erdosteine, an antioxidant, is expected to have an inhibitor potential against the disease. Rats were given one dose of bleomycin in pulmonary fibrosis groups and saline in controls. The first dose of oral Erdosteine (10 mg/kg/day) was given 2 days before the bleomycin injection to achieve the plateau level in blood and continued until killing. At day 14, fibrotic changes were evaluated, using Aschoft's criteria and lung hydroxyproline content. Bleomycin produced a fivefold increase in fibrosis score that was decreased by 87% by Erdosteine (P>0.001) and almost twofold increases in hydroxyproline content which were completely prevented by Erdosteine. Myeloperoxidase activities and MDA levels, which were significantly higher in the bleomycin group, were then significantly attenuated by Erdosteine. These results revealed that oral Erdosteine may prevent the development of acute pulmonary inflammation caused by bleomycin injection via the repression of neutrophil accumulation and lipid peroxidation, resulting in the inhibition of subsequent lung fibrosis.