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Shlomo Berliner - One of the best experts on this subject based on the ideXlab platform.
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p 121 morbid obesity association with increased Erythrocyte Adhesiveness aggregation in the peripheral blood potential pathophysiological implications
Diabetes Research and Clinical Practice, 2008Co-Authors: Itzhak Shapira, David Zeltser, Ori Rogowski, Shlomo BerlinerAbstract:Aims: We have shown recently that morbid obesity is associated with an enhanced degree of Erythrocyte Adhesiveness/aggregation in the peripheral venous blood. This phenomenon may have deleterious hemorheological effects. To clarify whether enhanced Erythrocyte Adhesiveness/aggregation is confined to morbid obesity or can be seen in the overweight/obese state in general. Methods: The subjects were 1021 participants of the Tel Aviv Medical Center Inflammation Survey who had the Erythrocyte Adhesiveness/aggregation test (ERYTHROSENSETM) measured using a simple slide test and image analysis as well as fibrinogen concentrations. Results: A significant age and anemia adjusted correlation between body mass index and degree of Erythrocyte Adhesiveness/aggregation was observed in both females (r=0.25, p<0.001) and males (r=0.15, p<0.002). However, when looking at the four body mass index categories of normal, overweight, obese and morbidly obese individuals, a significantly elevated degree of Erythrocyte Adhesiveness/aggregation was confined to the morbidly obese individuals. Conclusions: Significantly enhanced Erythrocyte Adhesiveness/aggregation is confined to morbidly obese individuals. This could have deleterious hemorheological consequences in morbidly obese subjects.
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P-121 Morbid obesity association with increased Erythrocyte Adhesiveness/aggregation in the peripheral blood.Potential pathophysiological implications
Diabetes Research and Clinical Practice, 2008Co-Authors: Itzhak Shapira, David Zeltser, Ori Rogowski, Shlomo BerlinerAbstract:Aims: We have shown recently that morbid obesity is associated with an enhanced degree of Erythrocyte Adhesiveness/aggregation in the peripheral venous blood. This phenomenon may have deleterious hemorheological effects. To clarify whether enhanced Erythrocyte Adhesiveness/aggregation is confined to morbid obesity or can be seen in the overweight/obese state in general. Methods: The subjects were 1021 participants of the Tel Aviv Medical Center Inflammation Survey who had the Erythrocyte Adhesiveness/aggregation test (ERYTHROSENSETM) measured using a simple slide test and image analysis as well as fibrinogen concentrations. Results: A significant age and anemia adjusted correlation between body mass index and degree of Erythrocyte Adhesiveness/aggregation was observed in both females (r=0.25, p
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inflammation related Erythrocyte aggregation in patients with inflammatory bowel disease
Digestive Diseases and Sciences, 2005Co-Authors: Liaz Zilberman, Meira V. Rozenblat, Itzhak Shapira, Nadir Arber, Ori Rogowski, Jacob Serov, Pinhas Halpern, Iris Dotan, Shlomo BerlinerAbstract:Chronic inflammation is associated with increased Erythrocyte Adhesiveness/aggregation. This might have deleterious effects on the microcirculatory flow and tissue oxygenation. We aimed to determine the degree of Erythrocyte Adhesiveness/aggregation in the peripheral blood of individuals with inflammatory bowel disease (IBD). Fifty-two patients (24 women and 28 men) with ulcerative colitis (UC) at a mean age of 44.0 ± 16.8 years and 96 patients (44 women and 52 men) with Crohn’s disease (CD) at a mean age of 38.0 ± 15.5 years, with various degrees of disease activity, were matched to normal controls. A simple slide test and image analysis were used to determine the degree of Erythrocyte Adhesiveness/aggregation. CD activity index (CDAI) was determined in patients with CD, while clinical colitis activity index was applied for patients with UC. A significant (P < 0.0005) increment in the degree of Erythrocyte Adhesiveness/aggregation was noted in both groups of IBD patients compared with matched control groups. This increment was evident even in individuals with a low index of disease activity and during remission. The highly significant correlation with the concentrations of fibrinogen suggests that the degree of Erythrocyte Adhesiveness/aggregation is an inflammation-related phenomenon. An enhanced state of Erythrocyte Adhesiveness/aggregation was noted in the peripheral blood of patients with IBD. This might have a deleterious effect on intestinal microcirculatory flow and tissue oxygenation.
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inflammation sensitive proteins and Erythrocyte aggregation in atherothrombosis
International Journal of Cardiology, 2005Co-Authors: Einor Ben Assayag, Tamar Mardi, Itzhak Shapira, Yelena Goldin, N. M. Bornstein, Tatiana Tolshinski, Yafa Vered, Vera Zakuth, Michael Burke, Shlomo BerlinerAbstract:Objective: To find the relative contribution of various inflammation-sensitive proteins including fibrinogen, immunoglobulins (IgG, IgM and IgA), ceruloplasmin and high sensitivity C-reactive protein (hs-CRP) to the induction and/or maintenance of enhanced Erythrocyte Adhesiveness/aggregation in the peripheral blood of individuals with atherothrombotic risk factors. Methods: The degree of Erythrocyte Adhesiveness/aggregation was determined by a simple slide test and image analysis. In addition, we measured various inflammation-sensitive protein levels including fibrinogen, ceruloplasmin, immunoglobulins and hs-CRP in a group of 234 individuals with atherothrombotic risk factors and healthy ones. Pearson partial correlations and multiple linear regression analysis were performed. Results: Fibrinogen was found to be the major protein contributing to the enhanced Erythrocyte Adhesiveness/aggregation, explaining 30% of the model. Fibrinogen and IgG together explained 32.4% of the model. Other inflammation-sensitive proteins did not reach statistical significance and were excluded from the model. Conclusions: Among inflammation-sensitive proteins measured in our cohort, fibrinogen is the dominant contributor to Erythrocyte Adhesiveness/aggregation in the peripheral blood of individuals with atherothrombotic risk factors and healthy ones. These findings may pave the way for the development of therapeutic strategies directed at the attenuation of Erythrocyte aggregability in individuals with atherothrombosis. D 2004 Elsevier Ireland Ltd. All rights reserved.
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Erythrocyte Adhesiveness aggregation a novel biomarker for the detection of low grade internal inflammation in individuals with atherothrombotic risk factors and proven vascular disease
American Heart Journal, 2005Co-Authors: Shlomo Berliner, Tamar Mardi, Ori Rogowski, Stanislav Aharonov, Tatiana Tolshinsky, Meira V. RozenblatAbstract:Background We have introduced a concept of using the Erythrocyte as a sensor for the detection of enhanced inflammation-sensitive protein concentrations. We presently evaluated the capability of this new biomarker to detect the presence of inflammation in individuals with a history of a vascular disease as opposed to individuals with atherothrombotic risk factors but no clinically evident vascular disease. Methods The degree of Erythrocyte Adhesiveness/aggregation was determined in the peripheral venous blood by using a simple blood test. Blood was drawn into a syringe containing sodium citrate and trickled onto a slide at an angle of 30°. The slides were than scanned by a blinded technician by using an image analyzer to determine the area that is covered by the Erythrocytes. Results One hundred fifty-six subjects (61 women and 95 men) of 2586 (1238 women and 1348 men) met the criteria of a definite vascular disease (history of stroke, myocardial infarction, coronary artery bypass grafting, or peripheral vascular disease). The degree of Erythrocyte aggregation was significantly ( P = .008) higher in men, but not in women, with vascular disease as opposed to these without a vascular disease. The results of receiver operating characteristic curve analysis confirmed the diagnostic superiority of the Erythrocyte aggregation biomarker over other commonly used markers of the acute phase in men. Similar results were obtained by using discriminant analysis. Finally, a significant correlation was found between the degree of Erythrocyte aggregation and other markers of the acute phase suggesting its relevance for the detection and quantitation of low-grade inflammation in individuals with atherothrombosis. Conclusion Erythrocyte Adhesiveness/aggregation may be a useful biomarker to detect internal inflammation in individuals with atherothrombosis.
David Zeltser - One of the best experts on this subject based on the ideXlab platform.
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p 121 morbid obesity association with increased Erythrocyte Adhesiveness aggregation in the peripheral blood potential pathophysiological implications
Diabetes Research and Clinical Practice, 2008Co-Authors: Itzhak Shapira, David Zeltser, Ori Rogowski, Shlomo BerlinerAbstract:Aims: We have shown recently that morbid obesity is associated with an enhanced degree of Erythrocyte Adhesiveness/aggregation in the peripheral venous blood. This phenomenon may have deleterious hemorheological effects. To clarify whether enhanced Erythrocyte Adhesiveness/aggregation is confined to morbid obesity or can be seen in the overweight/obese state in general. Methods: The subjects were 1021 participants of the Tel Aviv Medical Center Inflammation Survey who had the Erythrocyte Adhesiveness/aggregation test (ERYTHROSENSETM) measured using a simple slide test and image analysis as well as fibrinogen concentrations. Results: A significant age and anemia adjusted correlation between body mass index and degree of Erythrocyte Adhesiveness/aggregation was observed in both females (r=0.25, p<0.001) and males (r=0.15, p<0.002). However, when looking at the four body mass index categories of normal, overweight, obese and morbidly obese individuals, a significantly elevated degree of Erythrocyte Adhesiveness/aggregation was confined to the morbidly obese individuals. Conclusions: Significantly enhanced Erythrocyte Adhesiveness/aggregation is confined to morbidly obese individuals. This could have deleterious hemorheological consequences in morbidly obese subjects.
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P-121 Morbid obesity association with increased Erythrocyte Adhesiveness/aggregation in the peripheral blood.Potential pathophysiological implications
Diabetes Research and Clinical Practice, 2008Co-Authors: Itzhak Shapira, David Zeltser, Ori Rogowski, Shlomo BerlinerAbstract:Aims: We have shown recently that morbid obesity is associated with an enhanced degree of Erythrocyte Adhesiveness/aggregation in the peripheral venous blood. This phenomenon may have deleterious hemorheological effects. To clarify whether enhanced Erythrocyte Adhesiveness/aggregation is confined to morbid obesity or can be seen in the overweight/obese state in general. Methods: The subjects were 1021 participants of the Tel Aviv Medical Center Inflammation Survey who had the Erythrocyte Adhesiveness/aggregation test (ERYTHROSENSETM) measured using a simple slide test and image analysis as well as fibrinogen concentrations. Results: A significant age and anemia adjusted correlation between body mass index and degree of Erythrocyte Adhesiveness/aggregation was observed in both females (r=0.25, p
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The erythrosense as a real-time biomarker to reveal the presence of enhanced red blood cell aggregability in atherothrombosis.
American journal of therapeutics, 2005Co-Authors: Ori Rogowski, Meira V. Rozenblat, Shlomo Berliner, Varda Deutsch, David Zeltser, Daniel Justo, Jack Serov, Einor Ben-assayag, Anat Kessler, Vera ZakuthAbstract:Both anemia and inflammation might be present in individuals with atherothrombosis. We have evaluated the eventual influence of these 2 variables on the degree of Erythrocyte Adhesiveness/aggregation in the peripheral blood of 583 women and 402 men with various atherothrombotic risk factors and vascular events. It turned out that both anemia and inflammation (highly sensitive C-reactive protein concentrations) influence the degree of cell Adhesiveness/aggregation and that there is no interaction between them. Thus, the degree of Erythrocyte Adhesiveness/aggregation might have the diagnostic advantage of being enhanced in individuals with atherothrombosis who have inflammation and no anemia as well as those who have anemia and no inflammation. These findings might help to turn a phenomenon of hemorheological relevance into a diagnostic tool for the detection of individuals at risk of an acute ischemic event.
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Sex differences in the expression of haemorheological determinants in individuals with atherothrombotic risk factors and in apparently healthy people.
Heart (British Cardiac Society), 2004Co-Authors: David Zeltser, Meira V. Rozenblat, Tamar Mardi, Shlomo Berliner, Ori Rogowski, J. Serov, Dan Justo, D. Avitzour, Itzhak ShapiraAbstract:Background: Increased red cell aggregation can be detrimental, leading to slow capillary blood flow and tissue hypoxaemia. Sex differences in the degree of Erythrocyte Adhesiveness/aggregation in the peripheral blood have not been clearly shown. Objectives: To determine whether there are sex differences in the expression of Erythrocyte Adhesiveness/aggregation in the peripheral blood in individuals with atherothrombotic risk factors and in apparently healthy people. Methods: From a cohort of 965 participants in the Tel Aviv Medical Centre inflammation survey, 192 pairs of different sex were matched for age, body mass index, hip and waist circumferences, cardiovascular risk factors, and the intake of active cardiovascular drugs. Results: Women had an enhanced degree of red cell aggregation (p < 0.0005) as well as increased concentrations of inflammation sensitive proteins including fibrinogen and C reactive protein. Women had a lower haemoglobin concentration than men, but this did not affect the degree of Erythrocyte Adhesiveness/aggregation. Conclusions: The significant increase in red blood cell Adhesiveness/aggregation in the peripheral blood of women with atherothrombosis could be relevant to the more eventful course that some women experience during and following acute ischaemic disease.
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serum lipids as minor determinants of the degree of Erythrocyte Adhesiveness aggregation in the peripheral blood of individuals with low grade inflammation and moderately increased serum lipids
Clinical Hemorheology and Microcirculation, 2004Co-Authors: David Zeltser, Meira V. Rozenblat, Tamar Mardi, Yelena Goldin, J. Serov, S. Aharonov, O. Rogowski, T. Tulshinski, Dan Justo, Shlomo BerlinerAbstract:Both lipids and inflammation sensitive proteins have been reported to affect the aggregation of red blood cells yet their relative importance in this regard have not been determined. We have included high sensitive C-reactive protein, Erythrocyte sedimentation, fibrinogen concentrations as well as various serum lipid concentrations and the degree of Erythrocyte Adhesiveness/aggregation in the peripheral blood in a linear regression analysis. Partial Pearson correlation coefficients were included as well. In a group of 674 individuals with various atherosclerotic risk factors, low grade inflammation and moderately increased serum lipids, a relatively low correlation was noted between red blood cell Adhesiveness/aggregation and triglycerides concentrations. A negative correlation was noted for HDL cholesterol. None of the lipid variables turned significant in the regression analysis. In a group of individuals with atherosclerotic risk factors, low grade inflammation and moderately increased serum lipids, the degree of Erythrocyte Adhesiveness/aggregation in the peripheral blood correlates much better with the presence of inflammation sensitive proteins than with the presence of increased lipid concentrations.
Itzhak Shapira - One of the best experts on this subject based on the ideXlab platform.
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p 121 morbid obesity association with increased Erythrocyte Adhesiveness aggregation in the peripheral blood potential pathophysiological implications
Diabetes Research and Clinical Practice, 2008Co-Authors: Itzhak Shapira, David Zeltser, Ori Rogowski, Shlomo BerlinerAbstract:Aims: We have shown recently that morbid obesity is associated with an enhanced degree of Erythrocyte Adhesiveness/aggregation in the peripheral venous blood. This phenomenon may have deleterious hemorheological effects. To clarify whether enhanced Erythrocyte Adhesiveness/aggregation is confined to morbid obesity or can be seen in the overweight/obese state in general. Methods: The subjects were 1021 participants of the Tel Aviv Medical Center Inflammation Survey who had the Erythrocyte Adhesiveness/aggregation test (ERYTHROSENSETM) measured using a simple slide test and image analysis as well as fibrinogen concentrations. Results: A significant age and anemia adjusted correlation between body mass index and degree of Erythrocyte Adhesiveness/aggregation was observed in both females (r=0.25, p<0.001) and males (r=0.15, p<0.002). However, when looking at the four body mass index categories of normal, overweight, obese and morbidly obese individuals, a significantly elevated degree of Erythrocyte Adhesiveness/aggregation was confined to the morbidly obese individuals. Conclusions: Significantly enhanced Erythrocyte Adhesiveness/aggregation is confined to morbidly obese individuals. This could have deleterious hemorheological consequences in morbidly obese subjects.
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P-121 Morbid obesity association with increased Erythrocyte Adhesiveness/aggregation in the peripheral blood.Potential pathophysiological implications
Diabetes Research and Clinical Practice, 2008Co-Authors: Itzhak Shapira, David Zeltser, Ori Rogowski, Shlomo BerlinerAbstract:Aims: We have shown recently that morbid obesity is associated with an enhanced degree of Erythrocyte Adhesiveness/aggregation in the peripheral venous blood. This phenomenon may have deleterious hemorheological effects. To clarify whether enhanced Erythrocyte Adhesiveness/aggregation is confined to morbid obesity or can be seen in the overweight/obese state in general. Methods: The subjects were 1021 participants of the Tel Aviv Medical Center Inflammation Survey who had the Erythrocyte Adhesiveness/aggregation test (ERYTHROSENSETM) measured using a simple slide test and image analysis as well as fibrinogen concentrations. Results: A significant age and anemia adjusted correlation between body mass index and degree of Erythrocyte Adhesiveness/aggregation was observed in both females (r=0.25, p
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inflammation related Erythrocyte aggregation in patients with inflammatory bowel disease
Digestive Diseases and Sciences, 2005Co-Authors: Liaz Zilberman, Meira V. Rozenblat, Itzhak Shapira, Nadir Arber, Ori Rogowski, Jacob Serov, Pinhas Halpern, Iris Dotan, Shlomo BerlinerAbstract:Chronic inflammation is associated with increased Erythrocyte Adhesiveness/aggregation. This might have deleterious effects on the microcirculatory flow and tissue oxygenation. We aimed to determine the degree of Erythrocyte Adhesiveness/aggregation in the peripheral blood of individuals with inflammatory bowel disease (IBD). Fifty-two patients (24 women and 28 men) with ulcerative colitis (UC) at a mean age of 44.0 ± 16.8 years and 96 patients (44 women and 52 men) with Crohn’s disease (CD) at a mean age of 38.0 ± 15.5 years, with various degrees of disease activity, were matched to normal controls. A simple slide test and image analysis were used to determine the degree of Erythrocyte Adhesiveness/aggregation. CD activity index (CDAI) was determined in patients with CD, while clinical colitis activity index was applied for patients with UC. A significant (P < 0.0005) increment in the degree of Erythrocyte Adhesiveness/aggregation was noted in both groups of IBD patients compared with matched control groups. This increment was evident even in individuals with a low index of disease activity and during remission. The highly significant correlation with the concentrations of fibrinogen suggests that the degree of Erythrocyte Adhesiveness/aggregation is an inflammation-related phenomenon. An enhanced state of Erythrocyte Adhesiveness/aggregation was noted in the peripheral blood of patients with IBD. This might have a deleterious effect on intestinal microcirculatory flow and tissue oxygenation.
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inflammation sensitive proteins and Erythrocyte aggregation in atherothrombosis
International Journal of Cardiology, 2005Co-Authors: Einor Ben Assayag, Tamar Mardi, Itzhak Shapira, Yelena Goldin, N. M. Bornstein, Tatiana Tolshinski, Yafa Vered, Vera Zakuth, Michael Burke, Shlomo BerlinerAbstract:Objective: To find the relative contribution of various inflammation-sensitive proteins including fibrinogen, immunoglobulins (IgG, IgM and IgA), ceruloplasmin and high sensitivity C-reactive protein (hs-CRP) to the induction and/or maintenance of enhanced Erythrocyte Adhesiveness/aggregation in the peripheral blood of individuals with atherothrombotic risk factors. Methods: The degree of Erythrocyte Adhesiveness/aggregation was determined by a simple slide test and image analysis. In addition, we measured various inflammation-sensitive protein levels including fibrinogen, ceruloplasmin, immunoglobulins and hs-CRP in a group of 234 individuals with atherothrombotic risk factors and healthy ones. Pearson partial correlations and multiple linear regression analysis were performed. Results: Fibrinogen was found to be the major protein contributing to the enhanced Erythrocyte Adhesiveness/aggregation, explaining 30% of the model. Fibrinogen and IgG together explained 32.4% of the model. Other inflammation-sensitive proteins did not reach statistical significance and were excluded from the model. Conclusions: Among inflammation-sensitive proteins measured in our cohort, fibrinogen is the dominant contributor to Erythrocyte Adhesiveness/aggregation in the peripheral blood of individuals with atherothrombotic risk factors and healthy ones. These findings may pave the way for the development of therapeutic strategies directed at the attenuation of Erythrocyte aggregability in individuals with atherothrombosis. D 2004 Elsevier Ireland Ltd. All rights reserved.
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Erythrocyte Adhesiveness/aggregation: a novel biomarker for the detection of low-grade internal inflammation in individuals with atherothrombotic risk factors and proven vascular disease.
American heart journal, 2005Co-Authors: Shlomo Berliner, Meira V. Rozenblat, Tamar Mardi, Varda Deutsch, Ori Rogowski, Jack Serov, Dan Justo, Stanislav Aharonov, Tatiana Tolshinsky, Itzhak ShapiraAbstract:Background We have introduced a concept of using the Erythrocyte as a sensor for the detection of enhanced inflammation-sensitive protein concentrations. We presently evaluated the capability of this new biomarker to detect the presence of inflammation in individuals with a history of a vascular disease as opposed to individuals with atherothrombotic risk factors but no clinically evident vascular disease. Methods The degree of Erythrocyte Adhesiveness/aggregation was determined in the peripheral venous blood by using a simple blood test. Blood was drawn into a syringe containing sodium citrate and trickled onto a slide at an angle of 30°. The slides were than scanned by a blinded technician by using an image analyzer to determine the area that is covered by the Erythrocytes. Results One hundred fifty-six subjects (61 women and 95 men) of 2586 (1238 women and 1348 men) met the criteria of a definite vascular disease (history of stroke, myocardial infarction, coronary artery bypass grafting, or peripheral vascular disease). The degree of Erythrocyte aggregation was significantly ( P = .008) higher in men, but not in women, with vascular disease as opposed to these without a vascular disease. The results of receiver operating characteristic curve analysis confirmed the diagnostic superiority of the Erythrocyte aggregation biomarker over other commonly used markers of the acute phase in men. Similar results were obtained by using discriminant analysis. Finally, a significant correlation was found between the degree of Erythrocyte aggregation and other markers of the acute phase suggesting its relevance for the detection and quantitation of low-grade inflammation in individuals with atherothrombosis. Conclusion Erythrocyte Adhesiveness/aggregation may be a useful biomarker to detect internal inflammation in individuals with atherothrombosis.
Rivka Rotstein - One of the best experts on this subject based on the ideXlab platform.
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Telemedicine-based Application for the Detection of Inflammation in Pediatrics
Telemedicine Journal and e-Health, 2003Co-Authors: Joseph Urbach, Keren Elishkevitz, Rivka Rotstein, Meira V. Rozenblat, Tamar Mardi, David Brandski, Itzhak Shapira, Shlomo BerlinerAbstract:To assess the feasibility of using telemedicine for the diagnosis of inflammation in a pediatric population. The degree of Erythrocyte Adhesiveness/aggregation and the number of white blood cells on peripheral blood slides were evaluated using an image analysis system and transferred to an operator in a remote location. A highly significant correlation was found between the degrees of Erythrocyte Adhesiveness/aggregation as well as estimated white blood cell count determined by a remote operator and the objective measurements of both Erythrocyte sedimentation rate and white blood cell count. A reasonable estimate of both Erythrocyte sedimentation rate and white blood cell count can be obtained by using telemedicine in a group of children with acute infection/inflammation.
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the Erythrocyte Adhesiveness aggregation test eaat a new biomarker to reveal the presence of low grade subclinical smoldering inflammation in individuals with atherosclerotic risk factors
Atherosclerosis, 2002Co-Authors: Rivka Rotstein, Itzhak Shapira, David Zeltser, Tali Landau, Abraham Twig, Ardon Rubinstein, Michael Koffler, Daniel Justo, Doron Constantiner, Tamar MardiAbstract:Background: Multiple acute phase proteins and atherosclerotic risk factors increase the aggregability of Erythrocytes. Methods and results: We used a simple slide test and image analysis to determine the degree of Erythrocyte Adhesiveness/aggregation in the peripheral blood of 222 women and 221 men with no, one, two or more atherosclerotic risk factors. The degree of Erythrocyte Adhesiveness/aggregation correlated significantly with the concentration of commonly used variables of the acute phase response. We also showed that individuals with low Erythrocyte Adhesiveness/aggregation tend to be younger and to have fewer risk factors for atherosclerosis, including diabetes mellitus, hypertension, hyperlipidemia and smoking. Conclusions: The association between increased Erythrocyte Adhesiveness/aggregation, higher concentrations of acute phase proteins, and increased atherosclerotic risk factors points to a possible clinical applicability of the Erythrocyte Adhesiveness/aggregation test (EAAT) to reveal the presence of both low-grade subclinical smoldering inflammation and morbid biology in individuals with risk factors for atherosclerosis.
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A simple biomarker to exclude the presence of low grade inflammation in apparently healthy individuals.
Journal of cardiovascular risk, 2002Co-Authors: Shlomo Berliner, Tamar Mardi, Itzhak Shapira, David Zeltser, Nadir Arber, J. Serov, Stanislav Aharonov, Einor Ben Assayag, Rivka RotsteinAbstract:BACKGROUND Atherosclerosis is accompanied by a low grade inflammatory response. HYPOTHESIS To use Erythrocyte aggregability as a biomarker to exclude the presence of low grade inflammatory response in apparently healthy individuals. METHODS The Adhesiveness/aggregation of red blood cells was quantitated by using a simple slide test and image analysis. RESULTS We included 121 apparently healthy individuals and found a significant correlation between the degree of Erythrocyte Adhesiveness/aggregation and either the concentration of high sensitive CRP (r = 0.6, P < 0.001), Erythrocyte sedimentation rate (r = 0.5, P < 0.0001) or fibrinogen (r = 0.5, P < 0.0001). By using certain cutoff points for the Erythrocyte Adhesiveness/aggregation test we could define individuals with a very low grade inflammatory response. CONCLUSIONS By using this inexpensive and rapid assessment, we could clearly discriminate between individuals with a very low inflammatory response and those with a more intense one. This biomarker should be further evaluated as a possible screening test for use in large populations of apparently healthy individuals in whom the detection of low grade inflammation might contribute to guiding appropriate lifestyle modifications and therapeutic interventions.
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Prognostic implications of admission inflammatory profile in acute ischemic neurological events.
Acta neurologica Scandinavica, 2002Co-Authors: T. Anuk, Rivka Rotstein, Shlomo Berliner, Itzhak Shapira, Renato Fusman, David Zeltser, Nadir Arber, D. Avitzour, Einor Ben Assayag, N. M. BornsteinAbstract:Anuk T, Assayag EB, Rotstein R, Fusman R, Zeltser D, Berliner S, Avitzour D, Shapira I, Arber N, Bornstein NM. Prognostic implications of admission inflammatory profile in acute ischemic neurological events. Acta Neurol Scand 2002: 106: 196–199. © Blackwell Munksgaard 2002. Objective– To reveal the potential prognostic implications of admission inflammatory markers in patients with acute ischemic neurological events. Patients and methods– Sixty patients with an acute ischemic neurological event who were examined within 24 h from the appearance of symptomatology. We determined the high-sensitive C-reactive protein (hs-CRP) concentrations, Erythrocyte sedimentation rate (ESR), fibrinogen concentrations and degree of Erythrocyte Adhesiveness/aggregation. Results– A significant correlation was noted between baseline hs-CRP concentrations, ESR as well as Adhesiveness/aggregation and the outcome of the ischemic neurological event as determined by the modified Rankin scale 8–12 months following the insult. Conclusion– Admission inflammatory markers have long-term prognostic implications in patients with acute ischemic neurological events. These findings are relevant in view of the new therapeutic interventions now available for reducing the inflammatory response.
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Red blood cell Adhesiveness/aggregation, C-reactive protein, fibrinogen, and Erythrocyte sedimentation rate in healthy adults and in those with atherosclerotic risk factors.
The American journal of cardiology, 2002Co-Authors: Galit Fusman, Rivka Rotstein, Meira V. Rozenblat, Tamar Mardi, David Zeltser, Ardon Rubinstein, Michael Koffler, Daniel Justo, Esther Shabtai, Shlomo BerlinerAbstract:fl ammation in patients with atherosclerosis, 1 we questioned whether its presence in relatively high concentrations is associated with increased red blood cell (RBC) Adhesiveness/aggregation. Such an association might be relevant regarding the poorer prognosis that is observed in affected patients due to the unfavorable hemorheologic profi le that might exist in the presence of enhanced RBC Adhesiveness/aggregation. 2 The purpose of the present study was to confi rm the existence of enhanced RBC Adhesiveness/aggregation in adults with atherosclerotic risk factors and increased concentrations of hs-CRP. All the enrolled patients gave their consent for participation in this study, which was approved by the local ethics committee. We included subjects with atherosclerotic risk factors, including hypertension, diabetes mellitus, hyperlipidemia, and smoking, as well as apparently healthy institutional personnel who had no known risk factors. We excluded subjects who had underlying infl ammatory conditions (e.g., arthritis, infl ammatory bowel disease, and so on), those who had any infectious and/or infl ammatory condition such as tissue ischemia and/or infarction, and patients who had undergone any invasive procedure 6 months before study recruitment. Subjects who were on any steroidal or nonsteroidal anti-in fl ammatory medication (except for low doses [325 mg] of aspirin) were also excluded. The Erythrocyte Adhesiveness/aggregation test was performed by using a simple slide test. 3 In brief, venous blood was obtained from the antecubital vein into a plastic syringe containing sodium citrate 3.8% (0.25 ml citrate and 0.75 ml whole blood). The blood was mixed gently and 1 drop was applied to a glass slide kept at an angle of 30°, thus leaving a fi ne fi lm of blood on the slide. The slides were then analyzed by an image analyzer (INFLAMET, Infl amet Ltd., Tel Aviv, Israel). 4 A variable we labeled “vacuum radius” describes the space (micrometers) that is created between the aggregatedcells; the larger the space, the more intense the degree of Erythrocyte Adhesiveness/aggregation. 5 The RBC was performed by using the Coulter STKS autoanalyzer (Beckman Coulter, Nyon, Switzerland), the Erythrocyte sedimentation rate (ESR) by Westergen’s method, and quantitative fi brinogen by Clauss’ methods using a Sysmex 6000 autoanalyzer (Sysmex Corporation, Hyaga, Japan), whereas hsCRP was determined by using the Boering BNII nephelometer (DADE Boering, Marburg, Germany), as
Tamar Mardi - One of the best experts on this subject based on the ideXlab platform.
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inflammation sensitive proteins and Erythrocyte aggregation in atherothrombosis
International Journal of Cardiology, 2005Co-Authors: Einor Ben Assayag, Tamar Mardi, Itzhak Shapira, Yelena Goldin, N. M. Bornstein, Tatiana Tolshinski, Yafa Vered, Vera Zakuth, Michael Burke, Shlomo BerlinerAbstract:Objective: To find the relative contribution of various inflammation-sensitive proteins including fibrinogen, immunoglobulins (IgG, IgM and IgA), ceruloplasmin and high sensitivity C-reactive protein (hs-CRP) to the induction and/or maintenance of enhanced Erythrocyte Adhesiveness/aggregation in the peripheral blood of individuals with atherothrombotic risk factors. Methods: The degree of Erythrocyte Adhesiveness/aggregation was determined by a simple slide test and image analysis. In addition, we measured various inflammation-sensitive protein levels including fibrinogen, ceruloplasmin, immunoglobulins and hs-CRP in a group of 234 individuals with atherothrombotic risk factors and healthy ones. Pearson partial correlations and multiple linear regression analysis were performed. Results: Fibrinogen was found to be the major protein contributing to the enhanced Erythrocyte Adhesiveness/aggregation, explaining 30% of the model. Fibrinogen and IgG together explained 32.4% of the model. Other inflammation-sensitive proteins did not reach statistical significance and were excluded from the model. Conclusions: Among inflammation-sensitive proteins measured in our cohort, fibrinogen is the dominant contributor to Erythrocyte Adhesiveness/aggregation in the peripheral blood of individuals with atherothrombotic risk factors and healthy ones. These findings may pave the way for the development of therapeutic strategies directed at the attenuation of Erythrocyte aggregability in individuals with atherothrombosis. D 2004 Elsevier Ireland Ltd. All rights reserved.
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Erythrocyte Adhesiveness aggregation a novel biomarker for the detection of low grade internal inflammation in individuals with atherothrombotic risk factors and proven vascular disease
American Heart Journal, 2005Co-Authors: Shlomo Berliner, Tamar Mardi, Ori Rogowski, Stanislav Aharonov, Tatiana Tolshinsky, Meira V. RozenblatAbstract:Background We have introduced a concept of using the Erythrocyte as a sensor for the detection of enhanced inflammation-sensitive protein concentrations. We presently evaluated the capability of this new biomarker to detect the presence of inflammation in individuals with a history of a vascular disease as opposed to individuals with atherothrombotic risk factors but no clinically evident vascular disease. Methods The degree of Erythrocyte Adhesiveness/aggregation was determined in the peripheral venous blood by using a simple blood test. Blood was drawn into a syringe containing sodium citrate and trickled onto a slide at an angle of 30°. The slides were than scanned by a blinded technician by using an image analyzer to determine the area that is covered by the Erythrocytes. Results One hundred fifty-six subjects (61 women and 95 men) of 2586 (1238 women and 1348 men) met the criteria of a definite vascular disease (history of stroke, myocardial infarction, coronary artery bypass grafting, or peripheral vascular disease). The degree of Erythrocyte aggregation was significantly ( P = .008) higher in men, but not in women, with vascular disease as opposed to these without a vascular disease. The results of receiver operating characteristic curve analysis confirmed the diagnostic superiority of the Erythrocyte aggregation biomarker over other commonly used markers of the acute phase in men. Similar results were obtained by using discriminant analysis. Finally, a significant correlation was found between the degree of Erythrocyte aggregation and other markers of the acute phase suggesting its relevance for the detection and quantitation of low-grade inflammation in individuals with atherothrombosis. Conclusion Erythrocyte Adhesiveness/aggregation may be a useful biomarker to detect internal inflammation in individuals with atherothrombosis.
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Erythrocyte Adhesiveness/aggregation: a novel biomarker for the detection of low-grade internal inflammation in individuals with atherothrombotic risk factors and proven vascular disease.
American heart journal, 2005Co-Authors: Shlomo Berliner, Meira V. Rozenblat, Tamar Mardi, Varda Deutsch, Ori Rogowski, Jack Serov, Dan Justo, Stanislav Aharonov, Tatiana Tolshinsky, Itzhak ShapiraAbstract:Background We have introduced a concept of using the Erythrocyte as a sensor for the detection of enhanced inflammation-sensitive protein concentrations. We presently evaluated the capability of this new biomarker to detect the presence of inflammation in individuals with a history of a vascular disease as opposed to individuals with atherothrombotic risk factors but no clinically evident vascular disease. Methods The degree of Erythrocyte Adhesiveness/aggregation was determined in the peripheral venous blood by using a simple blood test. Blood was drawn into a syringe containing sodium citrate and trickled onto a slide at an angle of 30°. The slides were than scanned by a blinded technician by using an image analyzer to determine the area that is covered by the Erythrocytes. Results One hundred fifty-six subjects (61 women and 95 men) of 2586 (1238 women and 1348 men) met the criteria of a definite vascular disease (history of stroke, myocardial infarction, coronary artery bypass grafting, or peripheral vascular disease). The degree of Erythrocyte aggregation was significantly ( P = .008) higher in men, but not in women, with vascular disease as opposed to these without a vascular disease. The results of receiver operating characteristic curve analysis confirmed the diagnostic superiority of the Erythrocyte aggregation biomarker over other commonly used markers of the acute phase in men. Similar results were obtained by using discriminant analysis. Finally, a significant correlation was found between the degree of Erythrocyte aggregation and other markers of the acute phase suggesting its relevance for the detection and quantitation of low-grade inflammation in individuals with atherothrombosis. Conclusion Erythrocyte Adhesiveness/aggregation may be a useful biomarker to detect internal inflammation in individuals with atherothrombosis.
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Sex differences in the expression of haemorheological determinants in individuals with atherothrombotic risk factors and in apparently healthy people.
Heart (British Cardiac Society), 2004Co-Authors: David Zeltser, Meira V. Rozenblat, Tamar Mardi, Shlomo Berliner, Ori Rogowski, J. Serov, Dan Justo, D. Avitzour, Itzhak ShapiraAbstract:Background: Increased red cell aggregation can be detrimental, leading to slow capillary blood flow and tissue hypoxaemia. Sex differences in the degree of Erythrocyte Adhesiveness/aggregation in the peripheral blood have not been clearly shown. Objectives: To determine whether there are sex differences in the expression of Erythrocyte Adhesiveness/aggregation in the peripheral blood in individuals with atherothrombotic risk factors and in apparently healthy people. Methods: From a cohort of 965 participants in the Tel Aviv Medical Centre inflammation survey, 192 pairs of different sex were matched for age, body mass index, hip and waist circumferences, cardiovascular risk factors, and the intake of active cardiovascular drugs. Results: Women had an enhanced degree of red cell aggregation (p < 0.0005) as well as increased concentrations of inflammation sensitive proteins including fibrinogen and C reactive protein. Women had a lower haemoglobin concentration than men, but this did not affect the degree of Erythrocyte Adhesiveness/aggregation. Conclusions: The significant increase in red blood cell Adhesiveness/aggregation in the peripheral blood of women with atherothrombosis could be relevant to the more eventful course that some women experience during and following acute ischaemic disease.
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serum lipids as minor determinants of the degree of Erythrocyte Adhesiveness aggregation in the peripheral blood of individuals with low grade inflammation and moderately increased serum lipids
Clinical Hemorheology and Microcirculation, 2004Co-Authors: David Zeltser, Meira V. Rozenblat, Tamar Mardi, Yelena Goldin, J. Serov, S. Aharonov, O. Rogowski, T. Tulshinski, Dan Justo, Shlomo BerlinerAbstract:Both lipids and inflammation sensitive proteins have been reported to affect the aggregation of red blood cells yet their relative importance in this regard have not been determined. We have included high sensitive C-reactive protein, Erythrocyte sedimentation, fibrinogen concentrations as well as various serum lipid concentrations and the degree of Erythrocyte Adhesiveness/aggregation in the peripheral blood in a linear regression analysis. Partial Pearson correlation coefficients were included as well. In a group of 674 individuals with various atherosclerotic risk factors, low grade inflammation and moderately increased serum lipids, a relatively low correlation was noted between red blood cell Adhesiveness/aggregation and triglycerides concentrations. A negative correlation was noted for HDL cholesterol. None of the lipid variables turned significant in the regression analysis. In a group of individuals with atherosclerotic risk factors, low grade inflammation and moderately increased serum lipids, the degree of Erythrocyte Adhesiveness/aggregation in the peripheral blood correlates much better with the presence of inflammation sensitive proteins than with the presence of increased lipid concentrations.