The Experts below are selected from a list of 72 Experts worldwide ranked by ideXlab platform

Yoshimitsu Maede - One of the best experts on this subject based on the ideXlab platform.

  • Direct evidence for preferential multiplication of Babesia gibsoni in young Erythrocytes.
    Parasitology Research, 1993
    Co-Authors: Toshiyuki Murase, Masami Iwai, Yoshimitsu Maede
    Abstract:

    To clarify the effect of the age of host Erythrocytes on the multiplication ofBabesia parasites,B. gibsoni was Cultured together with reticulocytes, immature Erythrocytes, or mature Erythrocytes from dogs. Parasitemia reached peak levels (34.1%±15.8%) at cultivation day 8 in immature-Erythrocyte Culture, whereas the highest parasitemia attained in mature-cell Culture was only 3.6%±2.2% at day 5. These results clearly demonstrate thatB. gibsoni parasites preferentially invade and multiply in young Erythrocytes rather than in mature cells.

  • Direct evidence for preferential multiplication ofBabesia gibsoni in young Erythrocytes
    Parasitology Research, 1993
    Co-Authors: Toshiyuki Murase, Masami Iwai, Yoshimitsu Maede
    Abstract:

    To clarify the effect of the age of host Erythrocytes on the multiplication of Babesia parasites, B. gibsoni was Cultured together with reticulocytes, immature Erythrocytes, or mature Erythrocytes from dogs. Parasitemia reached peak levels (34.1%±15.8%) at cultivation day 8 in immature-Erythrocyte Culture, whereas the highest parasitemia attained in mature-cell Culture was only 3.6%±2.2% at day 5. These results clearly demonstrate that B. gibsoni parasites preferentially invade and multiply in young Erythrocytes rather than in mature cells.

Toshiyuki Murase - One of the best experts on this subject based on the ideXlab platform.

  • Direct evidence for preferential multiplication of Babesia gibsoni in young Erythrocytes.
    Parasitology Research, 1993
    Co-Authors: Toshiyuki Murase, Masami Iwai, Yoshimitsu Maede
    Abstract:

    To clarify the effect of the age of host Erythrocytes on the multiplication ofBabesia parasites,B. gibsoni was Cultured together with reticulocytes, immature Erythrocytes, or mature Erythrocytes from dogs. Parasitemia reached peak levels (34.1%±15.8%) at cultivation day 8 in immature-Erythrocyte Culture, whereas the highest parasitemia attained in mature-cell Culture was only 3.6%±2.2% at day 5. These results clearly demonstrate thatB. gibsoni parasites preferentially invade and multiply in young Erythrocytes rather than in mature cells.

  • Direct evidence for preferential multiplication ofBabesia gibsoni in young Erythrocytes
    Parasitology Research, 1993
    Co-Authors: Toshiyuki Murase, Masami Iwai, Yoshimitsu Maede
    Abstract:

    To clarify the effect of the age of host Erythrocytes on the multiplication of Babesia parasites, B. gibsoni was Cultured together with reticulocytes, immature Erythrocytes, or mature Erythrocytes from dogs. Parasitemia reached peak levels (34.1%±15.8%) at cultivation day 8 in immature-Erythrocyte Culture, whereas the highest parasitemia attained in mature-cell Culture was only 3.6%±2.2% at day 5. These results clearly demonstrate that B. gibsoni parasites preferentially invade and multiply in young Erythrocytes rather than in mature cells.

Manoj T. Duraisingh - One of the best experts on this subject based on the ideXlab platform.

  • In vitro genetic analysis of an Erythrocyte determinant of malaria infection.
    The Journal of infectious diseases, 2010
    Co-Authors: Amy K. Bei, Carlo Brugnara, Manoj T. Duraisingh
    Abstract:

    Invasion of Erythrocytes by Plasmodium falciparum is an obligatory step in the life cycle of the parasite. A major challenge is the unambiguous identification and characterization of host receptors. Because Erythrocytes lack nuclei, direct genetic analyses have been limited. In this work, we combined an in vitro Erythrocyte Culture system, which supports P. falciparum invasion and growth, with lentiviral transduction to knock down gene expression. We genetically demonstrate, in an isogenic background, that glycophorin A is required for efficient strain-specific parasite invasion. We establish the feasibility of in vitro systematic functional analysis of essential Erythrocyte determinants of malaria and Erythrocyte biology.

Masami Iwai - One of the best experts on this subject based on the ideXlab platform.

  • Direct evidence for preferential multiplication of Babesia gibsoni in young Erythrocytes.
    Parasitology Research, 1993
    Co-Authors: Toshiyuki Murase, Masami Iwai, Yoshimitsu Maede
    Abstract:

    To clarify the effect of the age of host Erythrocytes on the multiplication ofBabesia parasites,B. gibsoni was Cultured together with reticulocytes, immature Erythrocytes, or mature Erythrocytes from dogs. Parasitemia reached peak levels (34.1%±15.8%) at cultivation day 8 in immature-Erythrocyte Culture, whereas the highest parasitemia attained in mature-cell Culture was only 3.6%±2.2% at day 5. These results clearly demonstrate thatB. gibsoni parasites preferentially invade and multiply in young Erythrocytes rather than in mature cells.

  • Direct evidence for preferential multiplication ofBabesia gibsoni in young Erythrocytes
    Parasitology Research, 1993
    Co-Authors: Toshiyuki Murase, Masami Iwai, Yoshimitsu Maede
    Abstract:

    To clarify the effect of the age of host Erythrocytes on the multiplication of Babesia parasites, B. gibsoni was Cultured together with reticulocytes, immature Erythrocytes, or mature Erythrocytes from dogs. Parasitemia reached peak levels (34.1%±15.8%) at cultivation day 8 in immature-Erythrocyte Culture, whereas the highest parasitemia attained in mature-cell Culture was only 3.6%±2.2% at day 5. These results clearly demonstrate that B. gibsoni parasites preferentially invade and multiply in young Erythrocytes rather than in mature cells.

Sirlei Daffre - One of the best experts on this subject based on the ideXlab platform.

  • A tick cell line as a powerful tool to screen the antimicrobial susceptibility of the tick-borne pathogen Anaplasma marginale
    Experimental parasitology, 2020
    Co-Authors: Beatriz Iglesias Alonso, Elisa Silva Ventura, Eliane Esteves, Maria Fernanda Bandeira De Melo Galletti, Bruno Dall’agnol, João Ricardo Martins, Guilherme Marcondes Klafke, José Reck, Andréa Cristina Fogaça, Sirlei Daffre
    Abstract:

    Abstract Anaplasma marginale is the causative agent of the severe bovine anaplasmosis. The tick Rhipicephalus microplus is one of the main vectors of A. marginale in tropical and subtropical regions of the world. After the tick bite, the bacterium invades and proliferates within the bovine Erythrocytes leading to anemia, impairment of milk production and weight loss. In addition, infection can cause abortion and high mortality in areas of enzootic instability. Immunization with live and inactivated vaccines are employed to control acute bovine anaplasmosis. However, they do not prevent persistent infection. Consequently, infected animals, even if immunized, are still reservoirs of the bacterium and contribute to its dissemination. Antimicrobials are largely employed for the prophylaxis of bovine anaplasmosis. However, they are often used in sublethal doses which may select pre-existing resistant bacteria and induce genetic or phenotypic variations. Therefore, we propose a new standardized in vitro assay to evaluate the susceptibility of A. marginale strains to different antimicrobials. This tool will help health professionals to choose the more adequate treatment for each herd which will prevent the selection and spread of resistant strains. For that, we initially evaluated the antimicrobial susceptibility of two field isolates of A. marginale (Jaboticabal and Palmeira) infecting bovines. The least susceptible strain (Jaboticabal) was used for the standardization of an antimicrobial assay using a Culture of Ixodes scapularis-derived tick cell line, ISE6. Results showed that enrofloxacin (ENRO) at 0.25, 1 or 4 μg/mL and oxytetracycline (OTC) at 4 or 16 μg/mL are the most efficient treatments, followed by OTC at 1 μg/mL and imidocarb dipropionate (IMD) at 1 or 4 μg/mL. In addition, this proposed tool has technical advantages compared to the previously established bovine Erythrocyte Culture. Thereby, it may be used to guide cattle farmers to the correct use of antimicrobials. The choice of the most suitable antimicrobial is essential to eliminate persistent infections, prevent the spread of resistant strains and help controlling of bovine anaplasmosis.

  • A tick cell line as a powerful tool to screen the antimicrobial susceptibility of the tick-borne pathogen Anaplasma marginale
    2019
    Co-Authors: Beatriz Iglesias Alonso, Elisa Silva Ventura, Eliane Esteves, João Ricardo Martins, Guilherme Marcondes Klafke, José Reck, Andréa Cristina Fogaça, Maria F.b.m. Galletti, Bruno Dall'agnol, Sirlei Daffre
    Abstract:

    Abstract Background Anaplasma marginale is the causative agent of the severe bovine anaplasmosis. The tick Rhipicephalus microplus is one of the main vectors of A. marginale in tropical and subtropical regions of world. After the tick bite, the bacterium invades and proliferates within the bovine Erythrocytes, causing anemia and impairing milk production and weight gain. In addition, infection can cause abortion and high mortality in areas of enzootic instability. The immunization with live and inactivated vaccines are employed to the control acute bovine anaplasmosis. However, they do not prevent persistent infection. Therefore, infected animals, even if immunized, are reservoirs of the bacterium and contribute to the dissemination of the disease. Antimicrobials are also largely employed for the prophylaxis of bovine anaplasmosis. However, they are often used in subtlethal doses, what can select pre-existing resistant bacteria and induce genetic or phenotypic variations. Therefore, the standardization of an in vitro assay to evaluate the susceptibility of A. marginale strains to different antimicrobials is important to allow the prescription of the more effective treatment, preventing both the selection and spread of resistant strains. Results Initially the antimicrobial susceptibility of two field isolates of A. marginale (Jaboticabal and Palmeira) infecting bovines was evaluated. The least susceptible strain (Jaboticabal) was used for the standardization of an antimicrobial assay using a Culture of Ixodes scapularis-derived tick cell line, ISE6. Results showed that enrofloxacin (ENRO) at 0.25, 1 or 4 μg/mL and oxytetracycline (OTC) at 4 or 16 μg/mL are the most efficient treatments, followed by OTC at 1 μg/mL and imidocarb dipropionate (IMD) at 1 or 4 μg/mL. Conclusion In the current study, we present a new in vitro assay using a tick cell line to evaluate the susceptibility of A. marginale to antimicrobials. The maintenance of such Culture is much easier than the maintenance of bovine Erythrocyte Culture, which depends on continuous cell replacement. This assay may be used to guide cattle farmers to the correct use of antimicrobials. The choice of the most suitable antimicrobial is essential to eliminate persistent infections, preventing the spread of resistant strains and helping in the control of bovine anaplasmosis.