The Experts below are selected from a list of 126 Experts worldwide ranked by ideXlab platform
K. Miller - One of the best experts on this subject based on the ideXlab platform.
-
Sister-chromatid exchange in human B- and T-lymphocytes exposed to bleomycin, cyclophosphamide, and Ethyl Methanesulfonate.
Mutation research, 1991Co-Authors: K. MillerAbstract:Sister-chromatid exchange (SCE) frequencies were investigated in mitogen-stimulated cultures of highly purified human peripheral blood B- and T-lymphocytes exposed to bleomycin (BM), cyclophosphamide (CP), or Ethyl Methanesulfonate (EMS). In untreated controls, T-lymphocytes showed twice as many SCEs as B-lymphocytes. CP (with metabolic activation) and EMS significantly increased the SCE frequencies. EMS induced a similar, dose-dependent SCE increase in both cell populations, whereas CP induced more SCEs in T- than in B-lymphocytes. No clear SCE increase was found in B- and T-lymphocytes treated with BM.
Feng Zheng - One of the best experts on this subject based on the ideXlab platform.
-
determination of mEthyl Methanesulfonate and Ethyl Methanesulfonate in methanesulfonic acid by derivatization followed by high performance liquid chromatography with ultraviolet detection
IEEE Journal of Solid-state Circuits, 2017Co-Authors: Jie Zhou, Xiangyuan Zheng, Wenyuan Liu, Feng ZhengAbstract:Methanesulfonic acid is routinely used in pharmaceuticals but can contain potentially genotoxic impurities such as mEthyl Methanesulfonate and Ethyl Methanesulfonate. The aim of this study was to develop a simple high-performance liquid chromatography with ultraviolet detection method for determining mEthyl Methanesulfonate and Ethyl Methanesulfonate in methanesulfonic acid. Samples (250 mg) in water/acetonitrile (200 μL) were first combined with 10.0 mol/L sodium hydroxide solution (270 μL). Then they were mixed with 2.0 mg/mL N,N-diEthyldithiocarbamate (500 μL), diluted to 5 mL with N,N-dimEthylacetamide and allowed to react at 80°C for 1 h. The derivatives were analyzed using gradient high-performance liquid chromatography with ultraviolet detection (277 nm) and structurally elucidated by liquid chromatography with mass spectrometry. With acetonitrile/5 mmol/L ammonium acetate solution as the eluent and 1 mL/min as the flow rate on a C18 column, the derivatives were eluted at 10.6 and 14.8 min. Good linearity (correlation coefficients > 0.999) and low limits of quantitation (0.6 ppm) were obtained. The recoveries were in the range of 80-115% with relative standard deviation < 5.0%. Finally, the established method was successfully used for the determination of mEthyl Methanesulfonate and Ethyl Methanesulfonate in methanesulfonic acid.
-
Determination of mEthyl Methanesulfonate and Ethyl Methanesulfonate in methanesulfonic acid by derivatization followed by high‐performance liquid chromatography with ultraviolet detection
Journal of separation science, 2017Co-Authors: Jie Zhou, Xiangyuan Zheng, Wenyuan Liu, Feng ZhengAbstract:Methanesulfonic acid is routinely used in pharmaceuticals but can contain potentially genotoxic impurities such as mEthyl Methanesulfonate and Ethyl Methanesulfonate. The aim of this study was to develop a simple high-performance liquid chromatography with ultraviolet detection method for determining mEthyl Methanesulfonate and Ethyl Methanesulfonate in methanesulfonic acid. Samples (250 mg) in water/acetonitrile (200 μL) were first combined with 10.0 mol/L sodium hydroxide solution (270 μL). Then they were mixed with 2.0 mg/mL N,N-diEthyldithiocarbamate (500 μL), diluted to 5 mL with N,N-dimEthylacetamide and allowed to react at 80°C for 1 h. The derivatives were analyzed using gradient high-performance liquid chromatography with ultraviolet detection (277 nm) and structurally elucidated by liquid chromatography with mass spectrometry. With acetonitrile/5 mmol/L ammonium acetate solution as the eluent and 1 mL/min as the flow rate on a C18 column, the derivatives were eluted at 10.6 and 14.8 min. Good linearity (correlation coefficients > 0.999) and low limits of quantitation (0.6 ppm) were obtained. The recoveries were in the range of 80-115% with relative standard deviation < 5.0%. Finally, the established method was successfully used for the determination of mEthyl Methanesulfonate and Ethyl Methanesulfonate in methanesulfonic acid.
Oliver Hobert - One of the best experts on this subject based on the ideXlab platform.
-
analysis of multiple Ethyl Methanesulfonate mutagenized caenorhabditis elegans strains by whole genome sequencing
Genetics, 2010Co-Authors: Sumeet Sarin, Vincent Bertrand, Henry Bigelow, Alexander Boyanov, Maria Doitsidou, Richard J Poole, Surinder Narula, Oliver HobertAbstract:Whole-genome sequencing (WGS) of organisms displaying a specific mutant phenotype is a powerful approach to identify the genetic determinants of a plethora of biological processes. We have previously validated the feasibility of this approach by identifying a point-mutated locus responsible for a specific phenotype, observed in an Ethyl Methanesulfonate (EMS)-mutagenized Caenorhabditis elegans strain. Here we describe the genome-wide mutational profile of 17 EMS-mutagenized genomes as assessed with a bioinformatic pipeline, called MAQGene. Surprisingly, we find that while outcrossing mutagenized strains does reduce the total number of mutations, a striking mutational load is still observed even in outcrossed strains. Such genetic complexity has to be taken into account when establishing a causative relationship between genotype and phenotype. Even though unintentional, the 17 sequenced strains described here provide a resource of allelic variants in almost 1000 genes, including 62 premature stop codons, which represent candidate knockout alleles that will be of further use for the C. elegans community to study gene function.
Jie Zhou - One of the best experts on this subject based on the ideXlab platform.
-
determination of mEthyl Methanesulfonate and Ethyl Methanesulfonate in methanesulfonic acid by derivatization followed by high performance liquid chromatography with ultraviolet detection
IEEE Journal of Solid-state Circuits, 2017Co-Authors: Jie Zhou, Xiangyuan Zheng, Wenyuan Liu, Feng ZhengAbstract:Methanesulfonic acid is routinely used in pharmaceuticals but can contain potentially genotoxic impurities such as mEthyl Methanesulfonate and Ethyl Methanesulfonate. The aim of this study was to develop a simple high-performance liquid chromatography with ultraviolet detection method for determining mEthyl Methanesulfonate and Ethyl Methanesulfonate in methanesulfonic acid. Samples (250 mg) in water/acetonitrile (200 μL) were first combined with 10.0 mol/L sodium hydroxide solution (270 μL). Then they were mixed with 2.0 mg/mL N,N-diEthyldithiocarbamate (500 μL), diluted to 5 mL with N,N-dimEthylacetamide and allowed to react at 80°C for 1 h. The derivatives were analyzed using gradient high-performance liquid chromatography with ultraviolet detection (277 nm) and structurally elucidated by liquid chromatography with mass spectrometry. With acetonitrile/5 mmol/L ammonium acetate solution as the eluent and 1 mL/min as the flow rate on a C18 column, the derivatives were eluted at 10.6 and 14.8 min. Good linearity (correlation coefficients > 0.999) and low limits of quantitation (0.6 ppm) were obtained. The recoveries were in the range of 80-115% with relative standard deviation < 5.0%. Finally, the established method was successfully used for the determination of mEthyl Methanesulfonate and Ethyl Methanesulfonate in methanesulfonic acid.
-
Determination of mEthyl Methanesulfonate and Ethyl Methanesulfonate in methanesulfonic acid by derivatization followed by high‐performance liquid chromatography with ultraviolet detection
Journal of separation science, 2017Co-Authors: Jie Zhou, Xiangyuan Zheng, Wenyuan Liu, Feng ZhengAbstract:Methanesulfonic acid is routinely used in pharmaceuticals but can contain potentially genotoxic impurities such as mEthyl Methanesulfonate and Ethyl Methanesulfonate. The aim of this study was to develop a simple high-performance liquid chromatography with ultraviolet detection method for determining mEthyl Methanesulfonate and Ethyl Methanesulfonate in methanesulfonic acid. Samples (250 mg) in water/acetonitrile (200 μL) were first combined with 10.0 mol/L sodium hydroxide solution (270 μL). Then they were mixed with 2.0 mg/mL N,N-diEthyldithiocarbamate (500 μL), diluted to 5 mL with N,N-dimEthylacetamide and allowed to react at 80°C for 1 h. The derivatives were analyzed using gradient high-performance liquid chromatography with ultraviolet detection (277 nm) and structurally elucidated by liquid chromatography with mass spectrometry. With acetonitrile/5 mmol/L ammonium acetate solution as the eluent and 1 mL/min as the flow rate on a C18 column, the derivatives were eluted at 10.6 and 14.8 min. Good linearity (correlation coefficients > 0.999) and low limits of quantitation (0.6 ppm) were obtained. The recoveries were in the range of 80-115% with relative standard deviation < 5.0%. Finally, the established method was successfully used for the determination of mEthyl Methanesulfonate and Ethyl Methanesulfonate in methanesulfonic acid.
Shohei Mitani - One of the best experts on this subject based on the ideXlab platform.
-
characterization of mutations induced by Ethyl Methanesulfonate uv and trimEthylpsoralen in the nematode caenorhabditis elegans
Biochemical and Biophysical Research Communications, 2000Co-Authors: Keiko Gengyoando, Shohei MitaniAbstract:The genome project of the nematode Caenorhabditis elegans is completed. It is important and useful to disrupt nematode genes to know their function. We treated wild-type animals with potential candidates for mutagens for reverse genetics, EMS (Ethyl Methanesulfonate), short-wavelength UV, and long-wavelength UV in the presence of TMP (trimEthylpsoralen). We estimated forward mutation rates by counting the occurrence of a marker unc-22 mutation. We found that the forward mutation rate by TMP/UV could be comparable with EMS by improving the frequency one order higher than before. We next isolated mutants of another marker gene ben-1 and examined the probability for the deletion mutations by PCR and sequencing. Deletion mutations were found only by TMP/UV method, which suggested TMP/UV is the choice for deletion mutagenesis among these methods. As a pilot experiment, we could isolate actual deletion mutations at a much higher frequency than previously.