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Jia-zhen Yang - One of the best experts on this subject based on the ideXlab platform.

Luca Morini - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of Ethyl Glucuronide and Ethyl Sulfate in Calliphora Vicina as Potential Biomarkers for Ethanol Intake.
    Journal of Analytical Toxicology, 2016
    Co-Authors: Simonetta Lambiase, Angelo Groppi, Denise Gemmellaro, Luca Morini
    Abstract:

    : Ethyl glucuronide (EtG) and Ethyl Sulfate (EtS) are specific and sensitive biomarkers for the diagnosis of acute or chronic alcohol abuse. Due to postmortem alcohol production in biological tissues, they have recently been evaluated as potential biomarkers of ethanol ingestion. This in vitro study aimed to evaluate all developmental stages of the fly Calliphora vicina L. (Calliphoridae). Different pig substrates were used during the in vitro experiments to rear C. vicina Experiments were performed to: (i) assess the presence of EtG and EtS in larvae, pupae and insects; (ii) study variability due to the substrates characteristics; (iii) evaluate the possibility of false positives due to external alcohol contamination; and (iv) estimate the potential metabolism of ethanol by the insects. EtS was found in all of the samples where the standard was added to the substrate. Muscle provided the most reliable results. EtS was found in larvae, pupae and puparia. EtG and EtS were found in larvae and pupae collected from the body of an alcoholic found dead in his home. This study showed that maggots, pupae and puparia could be a useful matrix for the evaluation of antemortem alcohol ingestion.

  • Ethyl glucuronide and Ethyl Sulfate in placental and fetal tissues by liquid chromatography coupled with tandem mass spectrometry
    Analytical Biochemistry, 2011
    Co-Authors: Luca Morini, Angelo Groppi, Simona Pichini, Oscar Garciaalgar, Maria Falcon, Paolo Danesino, A Luna
    Abstract:

    Abstract The aim of this study was to develop a method for the determination of Ethyl-glucuronide (EtG) and Ethyl-Sulfate (EtS), two direct ethanol metabolites, in early placental and fetal human tissues, as potential biomarkers of transplacental ethanol transfer from the mother to the fetus. Placental and fetal tissue samples were obtained from women undergoing voluntary termination of pregnancy at 12 weeks of gestation. Samples were deproteinized and directly injected into a liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) system. Limits of detection of 13.0 and 23.0 pmol/g and lower limits of quantification of 22.0 and 40.0 pmol/g were reached for EtG and EtS, respectively. Inter- and intraday imprecision and accuracy were always lower than 15%. The method was applied to 70 samples (35 placentas and 35 fetal tissues). Of 35 samples, 4 samples collected from 4 women tested positive for EtG and EtS, always showing higher concentrations for EtG. The placenta/fetal tissue ratio for EtG was 2.9 ± 0.9, whereas EtS showed a ratio of 1.7 ± 0.7. Preliminary results suggest that these metabolites are present in both tissues. Further studies should now corroborate the hypothesis, not yet confirmed, that transplacental transfer of ethanol takes place not only for the parent compound but also for EtG and EtS.

  • population baseline of meconium Ethyl glucuronide and Ethyl Sulfate concentrations in newborns of nondrinking women in 2 mediterranean cohorts
    Therapeutic Drug Monitoring, 2010
    Co-Authors: Luca Morini, Angelo Groppi, Emilia Marchei, Federica Vagnarelli, Oscar Garcia Algar, Piergiorgio Zuccaro, Simona Pichini
    Abstract:

    Abstract:The detection of Ethyl glucuronide (EtG) and Ethyl Sulfate (EtS) in meconium has been investigated recently as an alternative to meconium fatty acid Ethyl esters (FAEEs) measurement as an objective estimate of prenatal alcohol exposure, independent of maternal self-reporting. We report the

  • Ethyl glucuronide and Ethyl Sulfate in meconium and hair potential biomarkers of intrauterine exposure to ethanol
    Forensic Science International, 2010
    Co-Authors: Luca Morini, Angelo Groppi, Emilia Marchei, Federica Vagnarelli, Garcia O Algar, Luisa Mastrobattista, Simona Pichini
    Abstract:

    Abstract This study investigated Ethyl glucuronide (EtG) and Ethyl Sulfate (EtS) concentration in meconium and in maternal and neonatal hair (HEtG and HFAEEs, respectively) as potential markers of intrauterine exposure to ethanol together with meconium fatty acid Ethyl esters (FAEEs) in a cohort of 99 mother–infant dyads, 49 coming from the Arcispedale of Reggio Emilia (Italy) and 50 from the Hospital del Mar of Barcelona (Spain). FAEEs, EtG and EtS were measured in meconium samples using liquid chromatography–tandem mass spectrometry. A head space-solid phase microextraction-gas chromatography–mass spectrometry was used to test HEtG and HFAEEs in hair samples from mothers and their newborns. Eighty-two meconium samples (82.8%) tested positive for EtG, 19 (19.2%) for EtS while 22 (22.2%) showed FAEEs levels higher than 2 nmol/g, the cut-off used to differentiate daily maternal ethanol consumption during pregnancy from occasional or no use. Although EtG and EtS in meconium did not correlate with total FAEEs concentration, a good correlation between EtG, EtS and Ethyl stearate was observed. Moreover, EtG correlated well with Ethyl palmitoleate, while EtS with Ethyl laurate, myristate and linolenate. Neither maternal nor neonatal hair appears as good predictors of gestational ethanol consumption and subsequent fetal exposure in these mother–infant dyads. In conclusion, these data show that meconium is so far the best matrix in evaluating intrauterine exposure to ethanol, with EtG and EtS being potentially good alternative biomarkers to FAEEs.

  • serum whole blood concentration ratio for Ethylglucuronide and Ethyl Sulfate
    Journal of Analytical Toxicology, 2009
    Co-Authors: Gudrun Hoiseth, Luca Morini, Aldo Polettini, Asbjorg S Christophersen, Lene Johnsen, Ritva Karinen, Jorg Morland
    Abstract:

    Serum/blood (S/B) concentration ratios for Ethyl glucuronide (EtG) and Ethyl Sulfate (EtS) are missing from the literature, and the aim of this study was to determine these ratios in samples from patients at admission to an alcohol rehabilitation clinic. Two blood samples were collected simultaneously, and EtG and EtS were analyzed in whole blood and serum, respectively, using a liquid chromatography‐mass spectrometry method. Separate calibration standards were prepared in both whole blood and serum for the calculation of whole blood and serum concentrations, respectively. Thirteen pairs of serum and whole blood were analyzed. The median S/B value for EtG was 1.69, and the range was 1.33‐1.90. For EtS, the median S/B ratio was 1.30, and the range was 1.08‐1.47. The S/B ratio was significantly lower for EtS than for EtG (p < 0.001). The higher concentrations of EtG and EtS in serum than in whole blood have to be considered when whole blood results obtained from forensic toxicology are compared to serum or plasma results from clinical laboratories.

Xingmei Lu - One of the best experts on this subject based on the ideXlab platform.

Hua-wei Li - One of the best experts on this subject based on the ideXlab platform.

Angelo Groppi - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of Ethyl Glucuronide and Ethyl Sulfate in Calliphora Vicina as Potential Biomarkers for Ethanol Intake.
    Journal of Analytical Toxicology, 2016
    Co-Authors: Simonetta Lambiase, Angelo Groppi, Denise Gemmellaro, Luca Morini
    Abstract:

    : Ethyl glucuronide (EtG) and Ethyl Sulfate (EtS) are specific and sensitive biomarkers for the diagnosis of acute or chronic alcohol abuse. Due to postmortem alcohol production in biological tissues, they have recently been evaluated as potential biomarkers of ethanol ingestion. This in vitro study aimed to evaluate all developmental stages of the fly Calliphora vicina L. (Calliphoridae). Different pig substrates were used during the in vitro experiments to rear C. vicina Experiments were performed to: (i) assess the presence of EtG and EtS in larvae, pupae and insects; (ii) study variability due to the substrates characteristics; (iii) evaluate the possibility of false positives due to external alcohol contamination; and (iv) estimate the potential metabolism of ethanol by the insects. EtS was found in all of the samples where the standard was added to the substrate. Muscle provided the most reliable results. EtS was found in larvae, pupae and puparia. EtG and EtS were found in larvae and pupae collected from the body of an alcoholic found dead in his home. This study showed that maggots, pupae and puparia could be a useful matrix for the evaluation of antemortem alcohol ingestion.

  • Ethyl glucuronide and Ethyl Sulfate in placental and fetal tissues by liquid chromatography coupled with tandem mass spectrometry
    Analytical Biochemistry, 2011
    Co-Authors: Luca Morini, Angelo Groppi, Simona Pichini, Oscar Garciaalgar, Maria Falcon, Paolo Danesino, A Luna
    Abstract:

    Abstract The aim of this study was to develop a method for the determination of Ethyl-glucuronide (EtG) and Ethyl-Sulfate (EtS), two direct ethanol metabolites, in early placental and fetal human tissues, as potential biomarkers of transplacental ethanol transfer from the mother to the fetus. Placental and fetal tissue samples were obtained from women undergoing voluntary termination of pregnancy at 12 weeks of gestation. Samples were deproteinized and directly injected into a liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) system. Limits of detection of 13.0 and 23.0 pmol/g and lower limits of quantification of 22.0 and 40.0 pmol/g were reached for EtG and EtS, respectively. Inter- and intraday imprecision and accuracy were always lower than 15%. The method was applied to 70 samples (35 placentas and 35 fetal tissues). Of 35 samples, 4 samples collected from 4 women tested positive for EtG and EtS, always showing higher concentrations for EtG. The placenta/fetal tissue ratio for EtG was 2.9 ± 0.9, whereas EtS showed a ratio of 1.7 ± 0.7. Preliminary results suggest that these metabolites are present in both tissues. Further studies should now corroborate the hypothesis, not yet confirmed, that transplacental transfer of ethanol takes place not only for the parent compound but also for EtG and EtS.

  • population baseline of meconium Ethyl glucuronide and Ethyl Sulfate concentrations in newborns of nondrinking women in 2 mediterranean cohorts
    Therapeutic Drug Monitoring, 2010
    Co-Authors: Luca Morini, Angelo Groppi, Emilia Marchei, Federica Vagnarelli, Oscar Garcia Algar, Piergiorgio Zuccaro, Simona Pichini
    Abstract:

    Abstract:The detection of Ethyl glucuronide (EtG) and Ethyl Sulfate (EtS) in meconium has been investigated recently as an alternative to meconium fatty acid Ethyl esters (FAEEs) measurement as an objective estimate of prenatal alcohol exposure, independent of maternal self-reporting. We report the

  • Ethyl glucuronide and Ethyl Sulfate in meconium and hair potential biomarkers of intrauterine exposure to ethanol
    Forensic Science International, 2010
    Co-Authors: Luca Morini, Angelo Groppi, Emilia Marchei, Federica Vagnarelli, Garcia O Algar, Luisa Mastrobattista, Simona Pichini
    Abstract:

    Abstract This study investigated Ethyl glucuronide (EtG) and Ethyl Sulfate (EtS) concentration in meconium and in maternal and neonatal hair (HEtG and HFAEEs, respectively) as potential markers of intrauterine exposure to ethanol together with meconium fatty acid Ethyl esters (FAEEs) in a cohort of 99 mother–infant dyads, 49 coming from the Arcispedale of Reggio Emilia (Italy) and 50 from the Hospital del Mar of Barcelona (Spain). FAEEs, EtG and EtS were measured in meconium samples using liquid chromatography–tandem mass spectrometry. A head space-solid phase microextraction-gas chromatography–mass spectrometry was used to test HEtG and HFAEEs in hair samples from mothers and their newborns. Eighty-two meconium samples (82.8%) tested positive for EtG, 19 (19.2%) for EtS while 22 (22.2%) showed FAEEs levels higher than 2 nmol/g, the cut-off used to differentiate daily maternal ethanol consumption during pregnancy from occasional or no use. Although EtG and EtS in meconium did not correlate with total FAEEs concentration, a good correlation between EtG, EtS and Ethyl stearate was observed. Moreover, EtG correlated well with Ethyl palmitoleate, while EtS with Ethyl laurate, myristate and linolenate. Neither maternal nor neonatal hair appears as good predictors of gestational ethanol consumption and subsequent fetal exposure in these mother–infant dyads. In conclusion, these data show that meconium is so far the best matrix in evaluating intrauterine exposure to ethanol, with EtG and EtS being potentially good alternative biomarkers to FAEEs.

  • liquid chromatography with tandem mass spectrometric detection for the measurement of Ethyl glucuronide and Ethyl Sulfate in meconium new biomarkers of gestational ethanol exposure
    Therapeutic Drug Monitoring, 2008
    Co-Authors: Luca Morini, Angelo Groppi, Emilia Marchei, Federica Vagnarelli, Manuela Pellegrini, Cristiana Stramesi, Oscar Garciaalgar, Roberta Pacifici, Simona Pichini
    Abstract:

    Abstract:A liquid chromatography tandem mass spectrometric (LC-MS/MS) method with postcolumn addition of acetonitrile for the determination of Ethyl glucuronide (EtG) and Ethyl Sulfate (EtS) in meconium was developed and validated using pentadeuterated EtG and pentadeuterated EtS as internal standar