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Véronique Bergougnoux - One of the best experts on this subject based on the ideXlab platform.
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affinity chromatography revealed 14 3 3 interactome of tomato solanum lycopersicum l during blue light induced de Etiolation
Journal of Proteomics, 2019Co-Authors: Petra Hlouskova, Martin Cerný, Nikola Kořinkova, Marketa Luklova, Eugenio G Minguet, Břetislav Brzobohatý, Petr Galuszka, Véronique BergougnouxAbstract:Abstract De-Etiolation is the first developmental process under light control allowing the heterotrophic seedling to become autotrophic. The phytohormones cytokinins (CKs) largely contribute to this process. Reversible phosphorylation is a key event of cell signaling, allowing proteins to become active or generating a binding site for specific protein interaction. 14-3-3 proteins regulate a variety of plant responses. The expression, hormonal regulation, and proteomic network under the control of 14-3-3s were addressed in tomato (Solanum lycopersicum L.) during blue light-induced photomorphogenesis. Two isoforms were specifically investigated due to their high expression during tomato de-Etiolation. The multidisciplinary approach demonstrated that TFT9 expression, but not TFT6, was regulated by CKs and identified cis-regulating elements required for this response. Our study revealed >130 potential TFT6/9 interactors. Their functional annotation predicted that TFTs might regulate the activity of proteins involved notably in cell wall strengthening or primary metabolism. Several potential interactors were also predicted to be CK-responsive. For the first time, the 14-3-3 interactome linked to de-Etiolation was investigated and evidenced that 14-3-3s might be involved in CK signaling pathway, cell expansion inhibition and steady-state growth rate establishment, and reprograming from heterotrophy to autotrophy. Biological significance Tomato (Solanum lycopersicum L.) is one of the most important vegetables consumed all around the world and represents probably the most preferred garden crop. Regulation of hypocotyl growth by light plays an important role in the early development of a seedling, and consequently the homogeneity of the culture. The present study focuses on the importance of tomato 14-3-3/TFT proteins in this process. We provide here the first report of 14-3-3 interactome in the regulation of light-induced de-Etiolation and subsequent photomorphogenesis. Our data provide new insights into light-induced de-Etiolation and open new horizons for dissecting the post-transcriptional regulations.
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a subtracted cdna library identifies genes up regulated during phot1 mediated early step of de Etiolation in tomato solanum lycopersicum l
BMC Genomics, 2016Co-Authors: Petra Hlouskova, Véronique BergougnouxAbstract:De-Etiolation is the switch from skoto- to photomorphogenesis, enabling the heterotrophic etiolated seedling to develop into an autotrophic plant. Upon exposure to blue light (BL), reduction of hypocotyl growth rate occurs in two phases: a rapid inhibition mediated by phototropin 1 (PHOT1) within the first 30–40 min of illumination, followed by the cryptochrome 1 (CRY1)-controlled establishment of the steady-state growth rate. Although some information is available for CRY1-mediated de-Etiolation, less attention has been given to the PHOT1 phase of de-Etiolation. We generated a subtracted cDNA library using the suppression subtractive hybridization method to investigate the molecular mechanisms of BL-induced de-Etiolation in tomato (Solanum lycopersicum L.), an economically important crop. We focused our interest on the first 30 min following the exposure to BL when PHOT1 is required to induce the process. Our library generated 152 expressed sequence tags that were found to be rapidly accumulated upon exposure to BL and consequently potentially regulated by PHOT1. Annotation revealed that biological functions such as modification of chromatin structure, cell wall modification, and transcription/translation comprise an important part of events contributing to the establishment of photomorphogenesis in young tomato seedlings. Our conclusions based on bioinformatics data were supported by qRT-PCR analyses the specific investigation of V-H+-ATPase during de-Etiolation in tomato. Our study provides the first report dealing with understanding the PHOT1-mediated phase of de-Etiolation. Using subtractive cDNA library, we were able to identify important regulatory mechanisms. The profound induction of transcription/translation, as well as modification of chromatin structure, is relevant in regard to the fact that the entry into photomorphogenesis is based on a deep reprograming of the cell. Also, we postulated that BL restrains the cell expansion by the rapid modification of the cell wall.
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Effect of blue light on endogenous isopentenyladenine and endoreduplication during photomorphogenesis and de-Etiolation of tomato (Solanum lycopersicum L.) seedlings.
PLOS ONE, 2012Co-Authors: Véronique Bergougnoux, David Zalabák, Michaela Jandová, Anika Wiese-klinkenberg, Ondřej Novak, Martin FellnerAbstract:Light is one of the most important factor influencing plant growth and development all through their life cycle. One of the well-known light-regulated processes is de-Etiolation, i.e. the switch from skotomorphogenesis to photomorphogenesis. The hormones cytokinins (CKs) play an important role during the establishment of photomorphogenesis as exogenous CKs induced photomorphogenesis of dark-grown seedlings. Most of the studies are conducted on the plant model Arabidopsis, but no or few information are available for important crop species, such as tomato (Solanum lycopersicum L.). In our study, we analyzed for the first time the endogenous CKs content in tomato hypocotyls during skotomorphogenesis, photomorphogenesis and de-Etiolation. For this purpose, two tomato genotypes were used: cv. Rutgers (wild-type; WT) and its corresponding mutant (7B-1) affected in its responses to blue light (BL). Using physiological and molecular approaches, we identified that the skotomorphogenesis is characterized by an endoreduplication-mediated cell expansion, which is inhibited upon BL exposure as seen by the accumulation of trancripts encoding CycD3, key regulators of the cell cycle. Our study showed for the first time that iP (isopentenyladenine) is the CK accumulated in the tomato hypocotyl upon BL exposure, suggesting its specific role in photomorphogenesis. This result was supported by physiological experiments and gene expression data. We propose a common model to explain the role and the relationship between CKs, namely iP, and endoreduplication during de-Etiolation and photomorphogenesis.
Azamal Husen - One of the best experts on this subject based on the ideXlab platform.
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Rejuvenation and Adventitious Rooting in Coppice-Shoot Cuttings of Tectona grandis as Affected by Stock-Plant Etiolation
American Journal of Plant Sciences, 2011Co-Authors: Azamal HusenAbstract:The effect of stock-plant Etiolation on adventitious rooting of single-node leafy cuttings (SNCs) made from coppice shoots from different age groups of donor plants were investigated for teak (Tectona grandis Linn. f.). When donor plants age were 1-, 2-, 3-, 4-, and 5-year-old, they were coppiced and maintained in the dark for Etiolation, while a parallel set was kept in normal light in an open environment. After 20 days, coppice shoots were made into SNCs which were cultured under intermittent mist for rooting. Stock-plant Etiolation significantly increased percent rooting, shoot and root length, and number of roots per SNC, but callusing at the base of SNCs decreased. Etiolated SNCs have shown 71.7% rooting, whereas for controls (non-etiolated), the response was 41.7%. The effect of aging on callusing, rooting and sprouting of etiolated and non-etiolated coppice shoots cuttings varied widely. Aging of donor plants decreased rooting and sprouting capability in SNCs and increased callusing at the base of etiolated cuttings. The results showed that stock-plant Etiolation in 1-, 2-, 3-, 4-, and 5-year-old donor plants hastened rooting potential by rejuvenation of coppice shoots.
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Stock-plant Etiolation causes drifts in total soluble sugars and anthraquinones, and promotes adventitious root formation in teak (Tectona grandis L. f.) coppice shoots
Plant Growth Regulation, 2008Co-Authors: Azamal HusenAbstract:The effects of stock-plant Etiolation on coppice-shoot growth, drifts in total soluble sugars and anthraquinones (AQs; C_14H_8O_2), and rooting potentiality of shoot cuttings were examined in Tectona grandis L. f. (clone FG1). When seedlings were one-year-old, they were coppiced and maintained in the dark for Etiolation, with a parallel set kept under natural light in an open environment. Coppice shoots were made into single-node leafy cuttings (SNCs), which were cultured under intermittent mist for rooting. These SNCs were treated with different concentrations of NAA (0, 2000 and 3000 mg l^−1). Etiolation significantly increased the coppice-shoot length, internode length, number of coppice shoots, number of leaves, number of nodes and total soluble sugars. The HPTLC analysis showed qualitative and quantitative differences in AQs in coppice shoots obtained from etiolated and non-etiolated stock plants. The study showed that AQs could be used as a marker for maturity and juvenility in teak. Stock-plant Etiolation caused a significant increase in percent rooting and sprouting, shoot length, number of shoots and number of leaves per SNC, but a decrease in callusing at the base of the SNC. NAA at 2000 and 3000 mg l^−1 had inhibitory effects on rooting and sprouting of SNCs. The result showed that stock-plant Etiolation fostered rooting by rejuvenating the coppice shoots.
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effect of serial bud grafting and Etiolation on rejuvenation and rooting cuttings of mature trees of tectona grandis linn f
Silvae Genetica, 2003Co-Authors: Azamal Husen, Mohinder PalAbstract:Summary An experiment was conducted to study the effect of serial bud grafting and Etiolation on rooting stem cuttings. Auxiliary buds taken from two clones of mature teak trees and were grafted on root stocks of 2-year-old seedlings in 1998 and again serially grafted on 2-year-old root stocks in 1999. The grafts were maintained under complete darkness to induce Etiolation. The etiolated shoot emerging from the grafted buds were made into mono-nodal, leafy cuttings, which were cultured under mist for rooting. Serial grafting and Etiolation did not affect rooting, but treatment with IBA in combination with serial grafting and Etiolation promoted root formation on the cuttings. All the control cuttings taken from lateral shoots after first or second grafting failed to root whereas, IBA-treated cuttings rooted very poorly after first grafting. However, rooting response in IBA-treated cuttings improved significantly after second grafting. Thus, serial grafting as well as Etiolation caused only partial rejuvenation of mature clones. The two clones differed in their response to serial grafting and Etiolation for induction of juvenility. Clone FG1 exhibited stronger rejuvenation, as indicated by more profuse rooting of the cuttings after second serial grafting. Clone FG11 exhibited comparatively weaker rejuvenation, which was indicated by less profuse rooting of the cuttings under similar conditions.
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Interactive Effect of Auxin and Etiolation on Adventitious Root formation in Cuttings of Tectona grandis Linn. F.
The Indian Forester, 2001Co-Authors: Azamal Husen, Mohinder PalAbstract:The effects of stock plant Etiolation on rooting behaviour of stem cuttings taken from one-year-old seedling of Teak ( Tectona grandis Linn. F.) were investigated. Except mean shoot diameter, Etiolation significantly increased mean shoot length, internodal length, number of coppice shoot, number of leaves, number of nodes and total sugar content of branches. Etiolation increased total soluble sugar content of branches. Studies have shown that Etiolation had pronounced effect on per cent sprouting, rooting, mean number of root length and mean number of root per cutting. Etiolated cutting have shown 100 per cent rooting, while nonetiolated (control) cutting under similar condition have given 46.60 per cent rooting. Application of NAA has significantly decreased rooting potential of even etiolated cuttings. Stock plant Etiolation and NAA have not shown significant interaction on adventitious root formation.
Petra Hlouskova - One of the best experts on this subject based on the ideXlab platform.
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affinity chromatography revealed 14 3 3 interactome of tomato solanum lycopersicum l during blue light induced de Etiolation
Journal of Proteomics, 2019Co-Authors: Petra Hlouskova, Martin Cerný, Nikola Kořinkova, Marketa Luklova, Eugenio G Minguet, Břetislav Brzobohatý, Petr Galuszka, Véronique BergougnouxAbstract:Abstract De-Etiolation is the first developmental process under light control allowing the heterotrophic seedling to become autotrophic. The phytohormones cytokinins (CKs) largely contribute to this process. Reversible phosphorylation is a key event of cell signaling, allowing proteins to become active or generating a binding site for specific protein interaction. 14-3-3 proteins regulate a variety of plant responses. The expression, hormonal regulation, and proteomic network under the control of 14-3-3s were addressed in tomato (Solanum lycopersicum L.) during blue light-induced photomorphogenesis. Two isoforms were specifically investigated due to their high expression during tomato de-Etiolation. The multidisciplinary approach demonstrated that TFT9 expression, but not TFT6, was regulated by CKs and identified cis-regulating elements required for this response. Our study revealed >130 potential TFT6/9 interactors. Their functional annotation predicted that TFTs might regulate the activity of proteins involved notably in cell wall strengthening or primary metabolism. Several potential interactors were also predicted to be CK-responsive. For the first time, the 14-3-3 interactome linked to de-Etiolation was investigated and evidenced that 14-3-3s might be involved in CK signaling pathway, cell expansion inhibition and steady-state growth rate establishment, and reprograming from heterotrophy to autotrophy. Biological significance Tomato (Solanum lycopersicum L.) is one of the most important vegetables consumed all around the world and represents probably the most preferred garden crop. Regulation of hypocotyl growth by light plays an important role in the early development of a seedling, and consequently the homogeneity of the culture. The present study focuses on the importance of tomato 14-3-3/TFT proteins in this process. We provide here the first report of 14-3-3 interactome in the regulation of light-induced de-Etiolation and subsequent photomorphogenesis. Our data provide new insights into light-induced de-Etiolation and open new horizons for dissecting the post-transcriptional regulations.
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a subtracted cdna library identifies genes up regulated during phot1 mediated early step of de Etiolation in tomato solanum lycopersicum l
BMC Genomics, 2016Co-Authors: Petra Hlouskova, Véronique BergougnouxAbstract:De-Etiolation is the switch from skoto- to photomorphogenesis, enabling the heterotrophic etiolated seedling to develop into an autotrophic plant. Upon exposure to blue light (BL), reduction of hypocotyl growth rate occurs in two phases: a rapid inhibition mediated by phototropin 1 (PHOT1) within the first 30–40 min of illumination, followed by the cryptochrome 1 (CRY1)-controlled establishment of the steady-state growth rate. Although some information is available for CRY1-mediated de-Etiolation, less attention has been given to the PHOT1 phase of de-Etiolation. We generated a subtracted cDNA library using the suppression subtractive hybridization method to investigate the molecular mechanisms of BL-induced de-Etiolation in tomato (Solanum lycopersicum L.), an economically important crop. We focused our interest on the first 30 min following the exposure to BL when PHOT1 is required to induce the process. Our library generated 152 expressed sequence tags that were found to be rapidly accumulated upon exposure to BL and consequently potentially regulated by PHOT1. Annotation revealed that biological functions such as modification of chromatin structure, cell wall modification, and transcription/translation comprise an important part of events contributing to the establishment of photomorphogenesis in young tomato seedlings. Our conclusions based on bioinformatics data were supported by qRT-PCR analyses the specific investigation of V-H+-ATPase during de-Etiolation in tomato. Our study provides the first report dealing with understanding the PHOT1-mediated phase of de-Etiolation. Using subtractive cDNA library, we were able to identify important regulatory mechanisms. The profound induction of transcription/translation, as well as modification of chromatin structure, is relevant in regard to the fact that the entry into photomorphogenesis is based on a deep reprograming of the cell. Also, we postulated that BL restrains the cell expansion by the rapid modification of the cell wall.
Tadeusz Rorat - One of the best experts on this subject based on the ideXlab platform.
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Genome-wide survey of B-box proteins in potato (Solanum tuberosum)—Identification, characterization and expression patterns during diurnal cycle, Etiolation and de-Etiolation
PloS one, 2017Co-Authors: Urszula Talar, Agnieszka Kiełbowicz-matuk, Jagoda Czarnecka, Tadeusz RoratAbstract:Plant B-box domain proteins (BBX) mediate many light-influenced developmental processes including seedling photomorphogenesis, seed germination, shade avoidance and photoperiodic regulation of flowering. Despite the wide range of potential functions, the current knowledge regarding BBX proteins in major crop plants is scarce. In this study, we identify and characterize the StBBX gene family in potato, which is composed of 30 members, with regard to structural properties and expression profiles under diurnal cycle, Etiolation and de-Etiolations. Based on domain organization and phylogenetic relationships, StBBX genes have been classified into five groups. Using real-time quantitative PCR, we found that expression of most of them oscillates following a 24-h rhythm; however, large differences in expression profiles were observed between the genes regarding amplitude and position of the maximal and minimal expression levels in the day/night cycle. On the basis of the time-of-day/time-of-night, we distinguished three expression groups specifically expressed during the light and two during the dark phase. In addition, we showed that the expression of several StBBX genes is under the control of the circadian clock and that some others are specifically associated with the Etiolation and de-Etiolation conditions. Thus, we concluded that StBBX proteins are likely key players involved in the complex diurnal and circadian networks regulating plant development as a function of light conditions and day duration.
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Classification of StBBX genes from Solanum tuberosum, cv. Desiree in terms of their expression in diurnal and circadian cycle and Etiolation and de-Etiolation conditions.
2017Co-Authors: Urszula Talar, Agnieszka Kiełbowicz-matuk, Jagoda Czarnecka, Tadeusz RoratAbstract:Classification of StBBX genes from Solanum tuberosum, cv. Desiree in terms of their expression in diurnal and circadian cycle and Etiolation and de-Etiolation conditions.
Martin Fellner - One of the best experts on this subject based on the ideXlab platform.
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Effect of blue light on endogenous isopentenyladenine and endoreduplication during photomorphogenesis and de-Etiolation of tomato (Solanum lycopersicum L.) seedlings.
PLOS ONE, 2012Co-Authors: Véronique Bergougnoux, David Zalabák, Michaela Jandová, Anika Wiese-klinkenberg, Ondřej Novak, Martin FellnerAbstract:Light is one of the most important factor influencing plant growth and development all through their life cycle. One of the well-known light-regulated processes is de-Etiolation, i.e. the switch from skotomorphogenesis to photomorphogenesis. The hormones cytokinins (CKs) play an important role during the establishment of photomorphogenesis as exogenous CKs induced photomorphogenesis of dark-grown seedlings. Most of the studies are conducted on the plant model Arabidopsis, but no or few information are available for important crop species, such as tomato (Solanum lycopersicum L.). In our study, we analyzed for the first time the endogenous CKs content in tomato hypocotyls during skotomorphogenesis, photomorphogenesis and de-Etiolation. For this purpose, two tomato genotypes were used: cv. Rutgers (wild-type; WT) and its corresponding mutant (7B-1) affected in its responses to blue light (BL). Using physiological and molecular approaches, we identified that the skotomorphogenesis is characterized by an endoreduplication-mediated cell expansion, which is inhibited upon BL exposure as seen by the accumulation of trancripts encoding CycD3, key regulators of the cell cycle. Our study showed for the first time that iP (isopentenyladenine) is the CK accumulated in the tomato hypocotyl upon BL exposure, suggesting its specific role in photomorphogenesis. This result was supported by physiological experiments and gene expression data. We propose a common model to explain the role and the relationship between CKs, namely iP, and endoreduplication during de-Etiolation and photomorphogenesis.