The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Gurunathan Jayaraman - One of the best experts on this subject based on the ideXlab platform.
-
quercetin 3 o rhamnoside from Euphorbia Hirta protects against snake venom induced toxicity
Biochimica et Biophysica Acta, 2016Co-Authors: Kadiyala Gopi, Kadali Renu, B S Vishwanath, K Anbarasu, Sivaraman Jayanthi, Gurunathan JayaramanAbstract:Abstract Background The plant Euphorbia Hirta is widely used against snake envenomations in rural areas and it was proved to be effective in animal models. Therefore, the scientific validation of its phytoconstituents for their antiophidian activity is aimed in the present study. Methods E. Hirta extract was subjected to bioactivity guided fractionation and the fractions that inhibited different enzyme activities of Naja naja venom in vitro was structurally characterized using UV, FT-IR, LC–MS and NMR spectroscopy. Edema, hemorrhage and lethality inhibition activity of the compound were studied in mice model. In addition, molecular docking and molecular dynamic simulations were also performed in silico. Results The bioactive fraction was identified as Quercetin-3-O-α-rhamnoside (QR, 448.38 Da). In vitro experiments indicated that protease, phospholipase-A2, hemolytic activity and hemorrhage inducing activity of the venom were inhibited completely at a ratio of 1:20 (venom: QR) w/w. At the same concentration, the edema ratio was drastically reduced from 187% to 107%. Significant inhibition (93%) of hyaluronidase activity was also observed at a slightly higher concentration of QR (1:50). Further, in in vivo analysis, QR significantly prolonged the survival time of mice injected with snake venom. Conclusion For the first time Quercetin-3-O-α-rhamnoside, isolated from E. Hirta, has been shown to exhibit anti-snake venom activity against Naja naja venom induced toxicity. General significance Exploring such multifunctional lead molecules with anti-venom activity would help in developing complementary medicine for snakebite treatments especially in rural areas where anti-snake venom is not readily available.
-
protective effect of Euphorbia Hirta and its components against snake venom induced lethality
Journal of Ethnopharmacology, 2015Co-Authors: Kadiyala Gopi, Kadali Renu, B S Vishwanath, Gurunathan JayaramanAbstract:Abstract Ethnopharmacological relevance Despite the use of snake anti-venom therapy, herbal medicine is still in practice to treat snakebites. Euphorbia Hirta is traditionally used as antidote for snakebites and also for numerous other ailments. However, the scientific evidence for its anti-snake venom property is still lacking. Materials and methods Methanolic extract of E. Hirta was evaluated for anti-venom activity under in vitro and ex vivo conditions. Histopathological changes in the vital organs of the mice were also monitored. UHPLC-SRM/MS was used to estimate the phenolic constituents whereas GC–MS analysis was performed to analyze the volatile metabolites present. The major compound was further evaluated for its contribution to the overall inhibitory potential of the extract. Results Methanolic extract of E. Hirta completely inhibited the venom enzymes under in vitro and reduced the edema ratio. The extract increased the survival time (>24 h) of mice which was further evidenced by histopathological analysis of vital organs. Phytochemical analysis revealed higher content of phenolic (144 mg/g extract) compounds in the extract. UHPLC-SRM/MS demonstrated that ellagic acid, gallic acid and quinic acid are the major phenolics whereas GC–MS analysis revealed pyrogallol as the major constituent (60.07%) among the volatile components of the extract. It was also shown that pyrogallol has the ability to differentially inhibit venom protease but not phospholipase A2. Conclusion The present study confirmed that E. Hirta methanolic extract was able to completely inhibit Naja naja venom induced toxicity under in vitro as well as ex vivo conditions, thus providing scientific evidence to its traditional use.
Kadiyala Gopi - One of the best experts on this subject based on the ideXlab platform.
-
quercetin 3 o rhamnoside from Euphorbia Hirta protects against snake venom induced toxicity
Biochimica et Biophysica Acta, 2016Co-Authors: Kadiyala Gopi, Kadali Renu, B S Vishwanath, K Anbarasu, Sivaraman Jayanthi, Gurunathan JayaramanAbstract:Abstract Background The plant Euphorbia Hirta is widely used against snake envenomations in rural areas and it was proved to be effective in animal models. Therefore, the scientific validation of its phytoconstituents for their antiophidian activity is aimed in the present study. Methods E. Hirta extract was subjected to bioactivity guided fractionation and the fractions that inhibited different enzyme activities of Naja naja venom in vitro was structurally characterized using UV, FT-IR, LC–MS and NMR spectroscopy. Edema, hemorrhage and lethality inhibition activity of the compound were studied in mice model. In addition, molecular docking and molecular dynamic simulations were also performed in silico. Results The bioactive fraction was identified as Quercetin-3-O-α-rhamnoside (QR, 448.38 Da). In vitro experiments indicated that protease, phospholipase-A2, hemolytic activity and hemorrhage inducing activity of the venom were inhibited completely at a ratio of 1:20 (venom: QR) w/w. At the same concentration, the edema ratio was drastically reduced from 187% to 107%. Significant inhibition (93%) of hyaluronidase activity was also observed at a slightly higher concentration of QR (1:50). Further, in in vivo analysis, QR significantly prolonged the survival time of mice injected with snake venom. Conclusion For the first time Quercetin-3-O-α-rhamnoside, isolated from E. Hirta, has been shown to exhibit anti-snake venom activity against Naja naja venom induced toxicity. General significance Exploring such multifunctional lead molecules with anti-venom activity would help in developing complementary medicine for snakebite treatments especially in rural areas where anti-snake venom is not readily available.
-
protective effect of Euphorbia Hirta and its components against snake venom induced lethality
Journal of Ethnopharmacology, 2015Co-Authors: Kadiyala Gopi, Kadali Renu, B S Vishwanath, Gurunathan JayaramanAbstract:Abstract Ethnopharmacological relevance Despite the use of snake anti-venom therapy, herbal medicine is still in practice to treat snakebites. Euphorbia Hirta is traditionally used as antidote for snakebites and also for numerous other ailments. However, the scientific evidence for its anti-snake venom property is still lacking. Materials and methods Methanolic extract of E. Hirta was evaluated for anti-venom activity under in vitro and ex vivo conditions. Histopathological changes in the vital organs of the mice were also monitored. UHPLC-SRM/MS was used to estimate the phenolic constituents whereas GC–MS analysis was performed to analyze the volatile metabolites present. The major compound was further evaluated for its contribution to the overall inhibitory potential of the extract. Results Methanolic extract of E. Hirta completely inhibited the venom enzymes under in vitro and reduced the edema ratio. The extract increased the survival time (>24 h) of mice which was further evidenced by histopathological analysis of vital organs. Phytochemical analysis revealed higher content of phenolic (144 mg/g extract) compounds in the extract. UHPLC-SRM/MS demonstrated that ellagic acid, gallic acid and quinic acid are the major phenolics whereas GC–MS analysis revealed pyrogallol as the major constituent (60.07%) among the volatile components of the extract. It was also shown that pyrogallol has the ability to differentially inhibit venom protease but not phospholipase A2. Conclusion The present study confirmed that E. Hirta methanolic extract was able to completely inhibit Naja naja venom induced toxicity under in vitro as well as ex vivo conditions, thus providing scientific evidence to its traditional use.
Kadali Renu - One of the best experts on this subject based on the ideXlab platform.
-
quercetin 3 o rhamnoside from Euphorbia Hirta protects against snake venom induced toxicity
Biochimica et Biophysica Acta, 2016Co-Authors: Kadiyala Gopi, Kadali Renu, B S Vishwanath, K Anbarasu, Sivaraman Jayanthi, Gurunathan JayaramanAbstract:Abstract Background The plant Euphorbia Hirta is widely used against snake envenomations in rural areas and it was proved to be effective in animal models. Therefore, the scientific validation of its phytoconstituents for their antiophidian activity is aimed in the present study. Methods E. Hirta extract was subjected to bioactivity guided fractionation and the fractions that inhibited different enzyme activities of Naja naja venom in vitro was structurally characterized using UV, FT-IR, LC–MS and NMR spectroscopy. Edema, hemorrhage and lethality inhibition activity of the compound were studied in mice model. In addition, molecular docking and molecular dynamic simulations were also performed in silico. Results The bioactive fraction was identified as Quercetin-3-O-α-rhamnoside (QR, 448.38 Da). In vitro experiments indicated that protease, phospholipase-A2, hemolytic activity and hemorrhage inducing activity of the venom were inhibited completely at a ratio of 1:20 (venom: QR) w/w. At the same concentration, the edema ratio was drastically reduced from 187% to 107%. Significant inhibition (93%) of hyaluronidase activity was also observed at a slightly higher concentration of QR (1:50). Further, in in vivo analysis, QR significantly prolonged the survival time of mice injected with snake venom. Conclusion For the first time Quercetin-3-O-α-rhamnoside, isolated from E. Hirta, has been shown to exhibit anti-snake venom activity against Naja naja venom induced toxicity. General significance Exploring such multifunctional lead molecules with anti-venom activity would help in developing complementary medicine for snakebite treatments especially in rural areas where anti-snake venom is not readily available.
-
protective effect of Euphorbia Hirta and its components against snake venom induced lethality
Journal of Ethnopharmacology, 2015Co-Authors: Kadiyala Gopi, Kadali Renu, B S Vishwanath, Gurunathan JayaramanAbstract:Abstract Ethnopharmacological relevance Despite the use of snake anti-venom therapy, herbal medicine is still in practice to treat snakebites. Euphorbia Hirta is traditionally used as antidote for snakebites and also for numerous other ailments. However, the scientific evidence for its anti-snake venom property is still lacking. Materials and methods Methanolic extract of E. Hirta was evaluated for anti-venom activity under in vitro and ex vivo conditions. Histopathological changes in the vital organs of the mice were also monitored. UHPLC-SRM/MS was used to estimate the phenolic constituents whereas GC–MS analysis was performed to analyze the volatile metabolites present. The major compound was further evaluated for its contribution to the overall inhibitory potential of the extract. Results Methanolic extract of E. Hirta completely inhibited the venom enzymes under in vitro and reduced the edema ratio. The extract increased the survival time (>24 h) of mice which was further evidenced by histopathological analysis of vital organs. Phytochemical analysis revealed higher content of phenolic (144 mg/g extract) compounds in the extract. UHPLC-SRM/MS demonstrated that ellagic acid, gallic acid and quinic acid are the major phenolics whereas GC–MS analysis revealed pyrogallol as the major constituent (60.07%) among the volatile components of the extract. It was also shown that pyrogallol has the ability to differentially inhibit venom protease but not phospholipase A2. Conclusion The present study confirmed that E. Hirta methanolic extract was able to completely inhibit Naja naja venom induced toxicity under in vitro as well as ex vivo conditions, thus providing scientific evidence to its traditional use.
B S Vishwanath - One of the best experts on this subject based on the ideXlab platform.
-
quercetin 3 o rhamnoside from Euphorbia Hirta protects against snake venom induced toxicity
Biochimica et Biophysica Acta, 2016Co-Authors: Kadiyala Gopi, Kadali Renu, B S Vishwanath, K Anbarasu, Sivaraman Jayanthi, Gurunathan JayaramanAbstract:Abstract Background The plant Euphorbia Hirta is widely used against snake envenomations in rural areas and it was proved to be effective in animal models. Therefore, the scientific validation of its phytoconstituents for their antiophidian activity is aimed in the present study. Methods E. Hirta extract was subjected to bioactivity guided fractionation and the fractions that inhibited different enzyme activities of Naja naja venom in vitro was structurally characterized using UV, FT-IR, LC–MS and NMR spectroscopy. Edema, hemorrhage and lethality inhibition activity of the compound were studied in mice model. In addition, molecular docking and molecular dynamic simulations were also performed in silico. Results The bioactive fraction was identified as Quercetin-3-O-α-rhamnoside (QR, 448.38 Da). In vitro experiments indicated that protease, phospholipase-A2, hemolytic activity and hemorrhage inducing activity of the venom were inhibited completely at a ratio of 1:20 (venom: QR) w/w. At the same concentration, the edema ratio was drastically reduced from 187% to 107%. Significant inhibition (93%) of hyaluronidase activity was also observed at a slightly higher concentration of QR (1:50). Further, in in vivo analysis, QR significantly prolonged the survival time of mice injected with snake venom. Conclusion For the first time Quercetin-3-O-α-rhamnoside, isolated from E. Hirta, has been shown to exhibit anti-snake venom activity against Naja naja venom induced toxicity. General significance Exploring such multifunctional lead molecules with anti-venom activity would help in developing complementary medicine for snakebite treatments especially in rural areas where anti-snake venom is not readily available.
-
protective effect of Euphorbia Hirta and its components against snake venom induced lethality
Journal of Ethnopharmacology, 2015Co-Authors: Kadiyala Gopi, Kadali Renu, B S Vishwanath, Gurunathan JayaramanAbstract:Abstract Ethnopharmacological relevance Despite the use of snake anti-venom therapy, herbal medicine is still in practice to treat snakebites. Euphorbia Hirta is traditionally used as antidote for snakebites and also for numerous other ailments. However, the scientific evidence for its anti-snake venom property is still lacking. Materials and methods Methanolic extract of E. Hirta was evaluated for anti-venom activity under in vitro and ex vivo conditions. Histopathological changes in the vital organs of the mice were also monitored. UHPLC-SRM/MS was used to estimate the phenolic constituents whereas GC–MS analysis was performed to analyze the volatile metabolites present. The major compound was further evaluated for its contribution to the overall inhibitory potential of the extract. Results Methanolic extract of E. Hirta completely inhibited the venom enzymes under in vitro and reduced the edema ratio. The extract increased the survival time (>24 h) of mice which was further evidenced by histopathological analysis of vital organs. Phytochemical analysis revealed higher content of phenolic (144 mg/g extract) compounds in the extract. UHPLC-SRM/MS demonstrated that ellagic acid, gallic acid and quinic acid are the major phenolics whereas GC–MS analysis revealed pyrogallol as the major constituent (60.07%) among the volatile components of the extract. It was also shown that pyrogallol has the ability to differentially inhibit venom protease but not phospholipase A2. Conclusion The present study confirmed that E. Hirta methanolic extract was able to completely inhibit Naja naja venom induced toxicity under in vitro as well as ex vivo conditions, thus providing scientific evidence to its traditional use.
Deborah Adejoke Adeniji - One of the best experts on this subject based on the ideXlab platform.
-
sperm motility and viability in west african dwarf rams treated with Euphorbia Hirta
International Journal of Morphology v.27 n.2 2009, 2019Co-Authors: M O Oyeyemi, S G Olukole, Bolanle Taiwo, Deborah Adejoke AdenijiAbstract:Four adult sexually matured and clinically healthy West African Dwarf (WAD) rams aged between 24 and 30 months were used for the study. The rams were first used as control and later as experimental animals upon being orally dosed with Euphorbia Hirta extract at 400mg/kg body weight for 14 days. Semen samples were collected from the rams a day after the administration of the plant extra and seven days after. The objective of the study was to investigate the effect of Euphorbia Hirta on the semen picture of WAD rams. There were significantly differences (P <0.05) in the semen picture as reflected in a reduction of sperm motility from 80% to 47.5% and live-dead ratio from 90.75% to 32.5% in the control and post-experimental stages of the study respectively. This indicates that the fertilization capacity and livability of spermatozoa were negatively affected. There were no significant differences in the values of body parameters measured across the stages of the study. The plant is therefore not recommended for medicinal purpose in male animals.
-
sperm motility and viability in west african dwarf rams treated with Euphorbia Hirta
International Journal of Morphology, 2009Co-Authors: M O Oyeyemi, S G Olukole, Bolanle Taiwo, Deborah Adejoke AdenijiAbstract:Cuatro carneros enanos adultos de Africa Occidental sexualmente maduros y clinicamente sanos, con edades comprendidas entre los 24 y 30 meses, fueron utilizados para este estudio. Los carneros fueron utilizados como control y, mas tarde, como animales de experimentacion al ser medicados por via oral con extracto de Euphorbia Hirta en 400mg/kg peso corporal durante 14 dias. Se recogieron muestras de semen de los carneros un dia despues de la administracion de la planta y siete dias despues. El objetivo del estudio fue investigar el efecto de Euphorbia Hirta en las imagenes de esperma de carneros enanos Africa Occidental. Hubo diferencias significativas (P <0,05) en la imagen del semen como reflejo de una reduccion de la motilidad espermatica del 80% al 47,5% y un ratio de vivos-muertos de 90,75% a 32,5% en la etapa control y despues de las fases experimentales del estudio, respectivamente. Esto indica que la capacidad de fertilizacion y calidad de vida de los espermatozoides fueron afectados negativamente. No hubo diferencias significativas en los valores de los parametros corporales medidos a traves de las etapas del estudio. La planta por tanto no es recomendable para fines medicinales en los animales machos.
