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Prachar J - One of the best experts on this subject based on the ideXlab platform.

Dipo Majekodunmi - One of the best experts on this subject based on the ideXlab platform.

Melissa Jacobs - One of the best experts on this subject based on the ideXlab platform.

Thomas U. Schwartz - One of the best experts on this subject based on the ideXlab platform.

  • Architectural Nucleoporins Nup157/170 and Nup133 Are Structurally Related and Descend from a Second Ancestral Element
    Journal of Biological Chemistry, 2009
    Co-Authors: James R.r. Whittle, Thomas U. Schwartz
    Abstract:

    Abstract The nuclear pore complex (NPC) constitutes one of the largest protein assemblies in the eukaryotic cell and forms the Exclusive Gateway to the nucleus. The stable, ∼15–20-MDa scaffold ring of the NPC is built from two multiprotein complexes arranged around a central 8-fold axis. Here we present crystal structures of two large architectural units, yNup170979–1502 and hNup107658–925·hNup133517–1156, each a constituent of one of the two multiprotein complexes. Conservation of domain arrangement and of tertiary structure suggests that Nup157/170 and Nup133 derived from a common ancestor. Together with the previously established ancestral coatomer element (ACE1), these two elements constitute the major α-helical building blocks of the NPC scaffold and define its branched, lattice-like architecture, similar to vesicle coats like COPII. We hypothesize that the extant NPC evolved early during eukaryotic evolution from a rudimentary structure composed of several identical copies of a few ancestral elements, later diversified and specified by gene duplication.

James R.r. Whittle - One of the best experts on this subject based on the ideXlab platform.

  • Architectural Nucleoporins Nup157/170 and Nup133 Are Structurally Related and Descend from a Second Ancestral Element
    Journal of Biological Chemistry, 2009
    Co-Authors: James R.r. Whittle, Thomas U. Schwartz
    Abstract:

    Abstract The nuclear pore complex (NPC) constitutes one of the largest protein assemblies in the eukaryotic cell and forms the Exclusive Gateway to the nucleus. The stable, ∼15–20-MDa scaffold ring of the NPC is built from two multiprotein complexes arranged around a central 8-fold axis. Here we present crystal structures of two large architectural units, yNup170979–1502 and hNup107658–925·hNup133517–1156, each a constituent of one of the two multiprotein complexes. Conservation of domain arrangement and of tertiary structure suggests that Nup157/170 and Nup133 derived from a common ancestor. Together with the previously established ancestral coatomer element (ACE1), these two elements constitute the major α-helical building blocks of the NPC scaffold and define its branched, lattice-like architecture, similar to vesicle coats like COPII. We hypothesize that the extant NPC evolved early during eukaryotic evolution from a rudimentary structure composed of several identical copies of a few ancestral elements, later diversified and specified by gene duplication.