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Zoilo Pires De Camargo - One of the best experts on this subject based on the ideXlab platform.
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A) Individual serum ELISA titers (1/T) of 28 sera from patients with PCM due to P. lutzii (blue) compared to individual serum ELISA titers (1/T) of 28 sera from patients with PCM due to P. brasiliensis (red) reacting with Ag CFA-Pl EPM 208 (12.5 µg/ml).
2014Co-Authors: Gregory Gegembauer, Rosane Christine Hahn, Leticia Mendes Araujo, Edy Firmina Pereira, Anderson Messias Rodrigues, Anamaria Mello Miranda Paniago, Zoilo Pires De CamargoAbstract:The P. lutzii sera presented ID + for P. lutzii CFA antigen and ID − for P. brasiliensis exoantigen (AgPbB339). The group of P. brasiliensis sera presented ID + for AgPbB339 antigen and ID − for P. lutzii CFA antigen. (*) represents P. lutzii gold standard serum and (**) represents sera that had a very weak cross reaction with P. brasiliensis exoantigen. B) Individual serum ELISA titers (1/T) of 28 sera from patients with PCM due to P. lutzii (blue) compared to individual serum ELISA titers (1/T) of 28 sera from patients with PCM due to P. brasiliensis (red) reacting with Ag Exo Pb B339 (10 µg/ml). The P. lutzii sera presented ID + for P. lutzii CFA antigen and ID − for P. brasiliensis exoantigen (AgPbB339). The group of P. brasiliensis sera presented ID + for AgPbB339 antigen and ID − for P. lutzii CFA antigen. (*) represents P. lutzii gold standard serum and (**) represents sera that had a very weak cross reaction with P. brasiliensis exoantigen. The same group of sera was used in A and B. C) ELISA median curves from sera from patients with PCM due to P. lutzii, PCM due to P. brasiliensis, and heterologous sera such as histoplasmosis, aspergillosis, sporotrichosis, and normal human serum. Antigen used was P. lutzii CFA (Ag CFA-Pl EPM 208 at 12.5 µg/ml). D) ELISA individual serum titers (1/T) of patients with PCM due to P. lutzii, P. brasiliensis, and heterologous sera such as Histoplasmosis, Aspergillosis, Sporotrichosis and normal human sera. By the end titer we could distinguish sera of PCM patients due to P. lutzii from sera of PCM due to P. brasiliensis and other mycotic diseases. Antigen used was P. lutzii CFA (Ag CFA-Pl EPM 208 at 12.5 µg/ml). Bars indicate the median.
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Serological and antigenic profiles of clinical isolates of Paracoccidioides spp. from Central Western Brazil
'Wiley', 2014Co-Authors: Queiroz Junior, Luiz De Padua, Zoilo Pires De Camargo, Tadano Tomoko, Rodrigues, Anderson Messias, Takarara, Doracilde Terumi, Gegembauer Gregory, Araujo, Leticia Mendes, Hahn, Rosane ChristineAbstract:Clinical Paracoccidioides spp. isolates from patients with paracoccidioidomycosis (PCM) in Mato Grosso, Brazil exhibit different patterns of serologic reactivity. the results observed for reactions of radial immunodiffusion against the commonly used Exoantigens containing a 43-kDa glycoprotein (gp43) suggest that this fungus exhibits major antigenic variability by geographic region. There is a phylogenetic gap between Paracoccidioides spp. isolates among different regions of Latin America. in particular, those from the central region of Brazil (i.e. Mato Grosso state) exhibit a lower rate of genetic similarity. We aimed at investigating the phylogenetic classification of clinical isolates of Paracoccidioides spp. in Central Brazil and the different antigenic profiles that produce. Exoantigens were obtained from five clinical isolates: two P. brasiliensis (Pb166 and Pb2880) and three P. lutzii (PL2875, PL9840, and PL2912). the protein/glycoprotein profiles of P. lutzii Exoantigens were different from each other. Isolate PL9840 exhibited the most distinct bands, and isolates PL2875 and PL2912 exhibited more diffuse bands and a very intense band between 50 and 60 kDa. P. brasiliensis isolates had similar protein profiles, exhibiting a low-intensity band at 220 kDa and a diffuse band between 50 and 60 kDa. P. lutzii isolates exhibit high species-specific antigen variability, which we have already been assessed in proteomic studies.Univ Fed Mato Grosso, Fac Med, Mycol Res Lab, UFMT, BR-78060900 Cuiaba, Mato Grosso, BrazilUniversidade Federal de São Paulo, Mycol Lab, UNIFESP, São Paulo, BrazilUniv Fed Mato Grosso, Julio Muller Univ Hosp, Cuiaba, Mato Grosso, BrazilState Dept Hlth, MT Lab, Cuiaba, Mato Grosso, BrazilUniversidade Federal de São Paulo, Mycol Lab, UNIFESP, São Paulo, BrazilWeb of Scienc
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paracoccidioides brasiliensis infection in dogs from western brazilian amazon
Pesquisa Veterinaria Brasileira, 2012Co-Authors: Andreia Correa Corte, Zoilo Pires De Camargo, Eiko Nakagawa Itano, Solange Maria Gennari, Marcelo B Labruna, Luis Marcelo Aranha Camargo, Roberta Lemos Freire, Mario Augusto OnoAbstract:The objective of the study was to evaluate Paracoccidioides brasiliensis infection in urban dogs from the municipality of Monte Negro, Rondonia, Western Brazilian Amazon. The serum samples (n=126) were analyzed by indirect ELISA and the immunodiffusion test using P. brasiliensis gp43 and exoantigen as antigens, respectively. A positivity of 54.8% was observed only in the ELISA test and no statistical difference was observed in the seroprevalence in relation to age or sex. This is the first paracoccidioidomycosis survey carried out with dogs from the Western Brazilian Amazon. The higher positivity rates of P. brasiliensis infection observed in this study suggest that veterinarians must be alert to detect new cases of natural disease in dogs living in paracoccidioidomycosis endemic areas.
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development and evaluation of a latex agglutination test for the serodiagnosis of paracoccidioidomycosis
Clinical and Vaccine Immunology, 2011Co-Authors: Fabiola Silveiragomes, Zoilo Pires De Camargo, Dayse Nogueira Sarmento, Thifany Mendes Pinto, Rosiane Ferreira Pimentel, Livia Barreto Nepomuceno, Elaine Patricia Tavares Do Espirito Santo, Maurimelia Mesquitadacosta, Silvia Helena MarquesdasilvaAbstract:Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin America. It is caused by the dimorphic fungus Paracoccidioides brasiliensis. The immunodiffusion (ID) test is one of the most widely used techniques for PCM serologic diagnosis due to the simplicity and low costs of its execution. However, it requires trained and qualified people to execute it. The purpose of this study was to evaluate a latex particle agglutination (LA) test for the detection of anti-P. brasiliensis antibodies by using pooled crude Exoantigens from the fungus. Fifty-one serum samples obtained from patients with PCM were tested. Positivity was observed in 84% (43/51) of these patients, and the agglutination patterns varied from small clumps with a cloudy background to large clumps with a clear background. The antibody titer reactivity ranged from 1:2 to 1:64. Cross-reactivity was observed in sera from patients with aspergillosis, histoplasmosis, and nonfungal disease. Serum samples obtained from healthy donors were not reactive. The sensitivity and specificity of the LA test were 84% and 81%, respectively. When comparing the LA test with the double-immunodiffusion test, we found an agreement of 92%. Further work is needed to improve the performance of the LA assay before it can be proposed as a reliable diagnostic tool, mainly in laboratories with little infrastructure.
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Serodiagnosis of sporotrichosis infection in cats by enzyme-linked immunosorbent assay using a specific antigen, SsCBF, and crude Exoantigens
'Elsevier BV', 2011Co-Authors: Fernandes, Geisa Ferreira, Lopes-bezerra, Leila Maria, Bernardes-engemann, Andrea Reis, Pacheco Schubach, Tania Maria, Galvao Dias, Maria Adelaide, Pereira, Sandro Antonio, Zoilo Pires De CamargoAbstract:The main objective of this study is to standardize an ELISA for the diagnosis of feline sporotrichosis. Sporothrix schenckii is the etiological agent of human and animal sporotrichosis. Cats may act as reservoirs for S. schenckii and can transmit the infection to humans by a bite or scratch. There are few methods for the serological diagnosis of fungal diseases in animals. in this paper, an ELISA test for the diagnosis of cat sporotrichosis is proposed, which detects S. schenckii-specific antibodies in feline sera. Two different kinds of antigens were used: SsCBF, a specific molecule from S. schenckii that consists of a Con A-binding fraction derived from a peptido-rhamnomannan component of the cell wall, and a S. schenckii crude exoantigen preparation. the ELISA was developed, optimized, and evaluated using sera from 30 cats with proven sporotrichosis (by culture isolation); 22 sera from healthy feral cats from a zoonosis center were used as negative controls. SsCBF showed 90% sensitivity and 96% specificity in ELISA; while crude Exoantigens demonstrated 96% sensitivity and 98% specificity. the ELISA assay described here would be a valuable screening tool for the detection of specifics. schenckii antibodies in cats with sporotrichosis. the assay is inexpensive, quick to perform, easy to interpret, and permits the diagnosis of feline sporotrichosis. (C) 2010 Elsevier B.V. All rights reserved.Universidade Federal de São Paulo, Disciplina Biol Celular, BR-04023062 São Paulo, BrazilUniv Estado Rio de Janeiro, Inst Biol IBRAG, Rio de Janeiro, BrazilFiocruz MS, Inst Oswaldo Cruz, Inst Pesquisa Clin Evandro Chagas, BR-21045900 Rio de Janeiro, BrazilCtr Zoonosis Prefeitura São Paulo, São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Biol Celular, BR-04023062 São Paulo, BrazilWeb of Scienc
J. H. L. Playfair - One of the best experts on this subject based on the ideXlab platform.
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Serological relationship of tumor necrosis factor-inducing Exoantigens of Plasmodium falciparum and Plasmodium vivax.
Infection and immunity, 1992Co-Authors: C. A. W. Bate, Janice Taverne, Nadira D. Karunaweera, Kamini N. Mendis, Dominic P. Kwiatkowski, J. H. L. PlayfairAbstract:Exoantigens of Plasmodium vivax-parasitized erythrocytes stimulated macrophages to secrete tumor necrosis factor, and antisera raised against the Exoantigens inhibited this secretion. The antisera also inhibited the activity of Plasmodium falciparum and Plasmodium yoelii Exoantigens, and conversely, antisera against the latter cross-reacted with the Exoantigens of P. vivax.
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Detoxified Exoantigens and phosphatidylinositol derivatives inhibit tumor necrosis factor induction by malarial Exoantigens. Infect. Immun
1992Co-Authors: C. A. W. Bate, T J. Taverne, J. H. L. PlayfairAbstract:We have previously shown that malaria parasites liberate Exoantigens which, through a phospholipid component, stimulate mouse macrophages to secrete tumor necrosis factor (TNF), which are toxic to D-galactosamine-sensitized mice, and which therefore might be involved in pathology. Plasmodium yoelii Exoantigens detoxified by dephosphorylation or digestion with lipases do not induce TNF production. However, these partial structures inhibited its production in response to the Exoantigens, although not to bacterial lipopolysaccharide (LPS). When pure phospholipids were tested in a macrophage assay, none stimulated the production of TNF, but phosphatidylinositol (PI) inhibited TNF induction by P. yoelii Exoantigens. Moreover, inositol monophosphate (IMP) was the only one of a number of monophosphate saccharides tested which was inhibitory; inositol was not. Macrophages pretreated with PI, IMP, or detoxified Exoantigens and then incubated with parasite Exoantigens also yielded much less TNF. PI, IMP, and lipase-digested Exoantigens of P. yoelii similarly inhibited the TNF-inducing activity of Exoantigens of the human parasites Plasmodium falciparum and Plasmodium vivax. Neither PI nor IMP diminished TNF production in response to LPS, in contrast to a platelet-activating factor antagonist [1-O-hexadecyl-2-acetyl-sn-glycero-3-phospho(N,N,N-tri-methyl)hexanolamine)J which inhibited both exoantigen- and LPS-induced production of TNF. We conclude that at least two different parts of the molecule are involved in the induction of TNF secretion by parasit
Paul E Verweij - One of the best experts on this subject based on the ideXlab platform.
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Specificity of a sandwich enzyme-linked immunosorbent assay for detecting Aspergillus galactomannan
Journal of Clinical Microbiology, 1997Co-Authors: C. M.a. Swanink, Jacques F.g.m. Meis, J. Peter Donnelly, Antonius J. M. M. Rijs, Paul E VerweijAbstract:The specificity of a sandwich enzyme-linked immunosorbent assay (ELISA) for detecting Aspergillus galactomannan was tested with Exoantigens of 29 fungi cultured from clinical specimens. Cross-reactivity was observed with Penicillium chrysogenum, Penicillium digitatum, and Paecilomyces variotii. Furthermore, 40 serum samples obtained from bacteremic patients with hematologic malignancies were retrospectively tested by sandwich ELISA. False-positive reactions with the serum were reproducible but did not correspond with the results of culture of specific microorganisms. Moreover, the microorganisms cultured from the blood showed no reactivity by the sandwich ELISA.
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Specificity of a sandwich enzyme-linked immunosorbent assay for detecting Aspergillus galactomannan
1997Co-Authors: C. M.a. Swanink, Jacques F.g.m. Meis, Antonius J. M. M. Rijs, Peter J. Donnelly, Paul E VerweijAbstract:The specificity of a sandwich enzyme-linked immunosorbent assay (ELISA) for detecting Aspergillus galac-tomannan was tested with Exoantigens of 29 fungi cultured from clinical specimens. Cross-reactivity was observed with Penicillium chrysogenum, Penicillium digitatum, and Paecilomyces variotii. Furthermore, 40 serum samples obtained from bacteremic patients with hematologic malignancies were retrospectively tested by sandwich ELISA. False-positive reactions with the serum were reproducible but did not correspond with the results of culture of specific microorganisms. Moreover, the microorganisms cultured from the blood showed no reactivity by the sandwich ELISA. Invasive aspergillosis is an opportunistic fungal infection which primarily affects the lungs. Among the patient popula-tions at greatest risk for infection are those with inadequate numbers of circulating neutrophils and those with defective neutrophil function. The crude mortality for patients with in-vasive aspergillosis is up to 95 % (4), which is partly due to the difficulty of diagnosing the infection at an early stage of dis-ease. Definitive evidence of infection can be obtained only b
C. A. W. Bate - One of the best experts on this subject based on the ideXlab platform.
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Serological relationship of tumor necrosis factor-inducing Exoantigens of Plasmodium falciparum and Plasmodium vivax.
Infection and immunity, 1992Co-Authors: C. A. W. Bate, Janice Taverne, Nadira D. Karunaweera, Kamini N. Mendis, Dominic P. Kwiatkowski, J. H. L. PlayfairAbstract:Exoantigens of Plasmodium vivax-parasitized erythrocytes stimulated macrophages to secrete tumor necrosis factor, and antisera raised against the Exoantigens inhibited this secretion. The antisera also inhibited the activity of Plasmodium falciparum and Plasmodium yoelii Exoantigens, and conversely, antisera against the latter cross-reacted with the Exoantigens of P. vivax.
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Detoxified Exoantigens and phosphatidylinositol derivatives inhibit tumor necrosis factor induction by malarial Exoantigens. Infect. Immun
1992Co-Authors: C. A. W. Bate, T J. Taverne, J. H. L. PlayfairAbstract:We have previously shown that malaria parasites liberate Exoantigens which, through a phospholipid component, stimulate mouse macrophages to secrete tumor necrosis factor (TNF), which are toxic to D-galactosamine-sensitized mice, and which therefore might be involved in pathology. Plasmodium yoelii Exoantigens detoxified by dephosphorylation or digestion with lipases do not induce TNF production. However, these partial structures inhibited its production in response to the Exoantigens, although not to bacterial lipopolysaccharide (LPS). When pure phospholipids were tested in a macrophage assay, none stimulated the production of TNF, but phosphatidylinositol (PI) inhibited TNF induction by P. yoelii Exoantigens. Moreover, inositol monophosphate (IMP) was the only one of a number of monophosphate saccharides tested which was inhibitory; inositol was not. Macrophages pretreated with PI, IMP, or detoxified Exoantigens and then incubated with parasite Exoantigens also yielded much less TNF. PI, IMP, and lipase-digested Exoantigens of P. yoelii similarly inhibited the TNF-inducing activity of Exoantigens of the human parasites Plasmodium falciparum and Plasmodium vivax. Neither PI nor IMP diminished TNF production in response to LPS, in contrast to a platelet-activating factor antagonist [1-O-hexadecyl-2-acetyl-sn-glycero-3-phospho(N,N,N-tri-methyl)hexanolamine)J which inhibited both exoantigen- and LPS-induced production of TNF. We conclude that at least two different parts of the molecule are involved in the induction of TNF secretion by parasit
Rosane Christine Hahn - One of the best experts on this subject based on the ideXlab platform.
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A) Individual serum ELISA titers (1/T) of 28 sera from patients with PCM due to P. lutzii (blue) compared to individual serum ELISA titers (1/T) of 28 sera from patients with PCM due to P. brasiliensis (red) reacting with Ag CFA-Pl EPM 208 (12.5 µg/ml).
2014Co-Authors: Gregory Gegembauer, Rosane Christine Hahn, Leticia Mendes Araujo, Edy Firmina Pereira, Anderson Messias Rodrigues, Anamaria Mello Miranda Paniago, Zoilo Pires De CamargoAbstract:The P. lutzii sera presented ID + for P. lutzii CFA antigen and ID − for P. brasiliensis exoantigen (AgPbB339). The group of P. brasiliensis sera presented ID + for AgPbB339 antigen and ID − for P. lutzii CFA antigen. (*) represents P. lutzii gold standard serum and (**) represents sera that had a very weak cross reaction with P. brasiliensis exoantigen. B) Individual serum ELISA titers (1/T) of 28 sera from patients with PCM due to P. lutzii (blue) compared to individual serum ELISA titers (1/T) of 28 sera from patients with PCM due to P. brasiliensis (red) reacting with Ag Exo Pb B339 (10 µg/ml). The P. lutzii sera presented ID + for P. lutzii CFA antigen and ID − for P. brasiliensis exoantigen (AgPbB339). The group of P. brasiliensis sera presented ID + for AgPbB339 antigen and ID − for P. lutzii CFA antigen. (*) represents P. lutzii gold standard serum and (**) represents sera that had a very weak cross reaction with P. brasiliensis exoantigen. The same group of sera was used in A and B. C) ELISA median curves from sera from patients with PCM due to P. lutzii, PCM due to P. brasiliensis, and heterologous sera such as histoplasmosis, aspergillosis, sporotrichosis, and normal human serum. Antigen used was P. lutzii CFA (Ag CFA-Pl EPM 208 at 12.5 µg/ml). D) ELISA individual serum titers (1/T) of patients with PCM due to P. lutzii, P. brasiliensis, and heterologous sera such as Histoplasmosis, Aspergillosis, Sporotrichosis and normal human sera. By the end titer we could distinguish sera of PCM patients due to P. lutzii from sera of PCM due to P. brasiliensis and other mycotic diseases. Antigen used was P. lutzii CFA (Ag CFA-Pl EPM 208 at 12.5 µg/ml). Bars indicate the median.
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is the geographical origin of a paracoccidioides brasiliensis isolate important for antigen production for regional diagnosis of paracoccidioidomycosis
Mycoses, 2010Co-Authors: Joao Batista, Zoilo Pires De Camargo, Geisa Ferreira Fernandes, A P Vicentini, Cor Jesus Fernandes Fontes, Rosane Christine HahnAbstract:Summary In this study, Exoantigens produced from two Paracoccidioides brasiliensis strains isolated in two different geographical areas were compared in terms of sensitivity and specificity in relation to paracoccidioidomycosis (PCM) diagnosis. Exoantigens from P. brasiliensis 550B (Ag 550B) isolated in the central-west region of Brazil (Mato Grosso State) and exoantigen produced from P. brasiliensis B-339 (Ag B-339) used in reference laboratories were compared by immunodiffusion (ID) tests. When Ag 550B was used in ID test against sera of patients from Mato Grosso and Sao Paulo, positivity was 92.3% and 41.3%, respectively. On the other hand, when Ag B-339 was tested with the same sera, positivity was 26.2% and 100%, respectively. These results suggest that differences in the antigenic composition probably related to phylogenetic peculiarities in P. brasiliensis isolates from the central-western region of Brazil should be considered in the diagnosis of PCM.