The Experts below are selected from a list of 213 Experts worldwide ranked by ideXlab platform
Martin L Yarmush - One of the best experts on this subject based on the ideXlab platform.
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in situ metabolic flux analysis to quantify the liver metabolic response to Experimental Burn injury
Biotechnology and Bioengineering, 2011Co-Authors: Marialouisa Izamis, Nripen Sharma, Basak E Uygun, Robert M Bieganski, Nima Saeidi, Yaakov Nahmias, Korkut Uygun, Martin L YarmushAbstract:Trauma such as Burns induces a hypermetabolic response associated with altered central carbon and nitrogen metabolism. The liver plays a key role in these metabolic changes; however, studies to date have evaluated the metabolic state of liver using ex vivo perfusions or isotope labeling techniques targeted to specific pathways. Herein, we developed a unique mass balance approach to characterize the metabolic state of the liver in situ, and used it to quantify the metabolic changes to Experimental Burn injury in rats. Rats received a sham (control uninjured), 20% or 40% total body surface area (TBSA) scald Burn, and were allowed to develop a hypermetabolic response. One day prior to evaluation, all animals were fasted to deplete glycogen stores. Four days post-Burn, blood flow rates in major vessels of the liver were measured, and blood samples harvested. We combined measurements of metabolite concentrations and flow rates in the major vessels entering and leaving the liver with a steady-state mass balance model to generate a quantitative picture of the metabolic state of liver. The main findings were: (1) Sham-Burned animals exhibited a gluconeogenic pattern, consistent with the fasted state; (2) the 20% TBSA Burn inhibited gluconeogenesis and exhibited glycolytic-like features with very few other significant changes; (3) the 40% TBSA Burn, by contrast, further enhanced gluconeogenesis and also increased amino acid extraction, urea cycle reactions, and several reactions involved in oxidative phosphorylation. These results suggest that increasing the severity of injury does not lead to a simple dose-dependent metabolic response, but rather leads to qualitatively different responses.
Lydia Masako Ferreira - One of the best experts on this subject based on the ideXlab platform.
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development of Experimental in vitro Burn model
Acta Cirurgica Brasileira, 2014Co-Authors: Ana Carolina Morais Fernandes, Jeronimo Pereira De Franca, Silvana Gaiba, Antonio Carlos Aloise, Andrea Fernandes De Oliveira, Andrea Aparecida De Fatima Souza Moraes, Lucimar Pereira De Franca, Lydia Masako FerreiraAbstract:PURPOSE: To propose an Experimental Burn model in NIH-3T3 cell line.METHODS:Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and imunofluorescence.RESULTS:The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro Burn model, using the cell line NIH-3T3 was observed drastic cellular injury with significant changes in cell viability and activity. It showed drastically modified cell morphology with altered membrane, cytoskeleton and nucleus, and low cellularity compared to the control group.CONCLUSION:The Burn model in vitro using the cell line NIH-3T3 was reproductive and efficient. This Burn model was possible to determine significant changes in cell activity and decreased viability, with drastic change in morphology, cell lysis and death.
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human keratinocytes cultured on collagen matrix used as an Experimental Burn model
ePlasty, 2007Co-Authors: C S Sobral, Alfredo Gragnani, Jeffrey R Morgan, Lydia Masako FerreiraAbstract:Background: In Experimental models in vivo, it is difficult to characterize the effect of thermal Burns on epidermal keratinocytes. Since the response to thermal injury involves several systemic mechanisms, especially because of the stimulus to coagulation and inflammatory cascades, it becomes hard to evaluate the specific effect of thermal Burns on keratinocytes. The aim of this study is to propose the use of human keratinocytes cultured on collagen matrix as an in vitro Experimental Burn model. Methods: Human keratinocytes derived from neonatal foreskins were isolated and cultured following standard methods. All experiments used the same keratinocyte lineage and were carried out in triplicate. Initially, gels of collagen and Matrigel were prepared. For each gel, 2 × 106 keratinocytes were seeded and cultured to form stratified epithelia. Following, Burn wounds were induced at 170°C. Results: Keratinocytes were cultured on collagen-coated Millicell membranes. Stratified epithelia were formed and Burned on the seventh day after the cultures were raised to the air-liquid interface. The Burn procedure is reproducible and can be easily executed. Conclusion: The proposed model can be used to study the effects of induced Burn wounds on keratinocytes in a specific way.
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inhibition of proliferation of pseudomonas aeruginosa by kgf in an Experimental Burn model using human cultured keratinocytes
Burns, 2007Co-Authors: C S Sobral, Alfredo Gragnani, Jeffrey R Morgan, Lydia Masako FerreiraAbstract:Abstract Experimental models showed the ability of Pseudomonas aeruginosa to interact with epidermal keratinocytes [Green H, Kehinde O, Thomas J. Growth of cultured human epidermal cells into multiple epithelia suitable for grafting. Proc Natl Acad Sci USA 1979;76(11):5665–8], stimulating these cells to produce specific peptides that start an immunological chain reaction in the epidermis [O’Connor NE, Mulliken JB, Banks-Schlegel S, Kehinde O, Green H. Grafting of Burns with cultured epithelium prepared from autologous epidermal cells. Lancet 1981;(1):75–8]. The immune reaction causes the release of cytokines and growth factors. The objective of this study was to test whether the presence of keratinocyte growth factor (KGF) alters P. aeruginosa proliferation in an Experimental Burn model. Methods Human keratinocytes derived from neonatal foreskins were isolated and cultured following standard methods [Gallico III, GG, O’Connor NE, Compton CC, Kehinde O, Green H. Permanent coverage of large Burn wounds with autologous cultured human epithelium. N Engl J Med 1984;311(7):448–51]. Some of these cells were genetically modified to produce KGF, and the other cells were supplemented with different KGF concentrations in the culture media. Both groups of keratinocytes were seeded in collagen matrices and cultured to form stratified epithelia. A hot plate was used to produce Burn defects. Each matrix was inoculated with luminescent P. aeruginosa strain. Experiments were made using keratinocytes without KGF, keratinocytes supplemented with different concentrations of KGF, and keratinocytes genetically modified to produce KGF. Statistical analyses were made using Wilcoxon paired test. Results When KGF was added to P. aeruginosa in the presence of keratinocytes, bacterial growth was inhibited, and the same was observed when genetically modified keratinocytes were used. Conclusion Many studies have been done on KGF, where its known properties were defined as a mitogen for keratinocytes [Munster AM. Cultured skin for massive Burns: a prospective, controlled trial. Ann Surg 1996;224(3):372–7]. This method allows for a qualitative and quantitative evaluation in real time of the bacterial growth in wound sites after bacterial inoculation. KGF was involved in the reduction of bacterial viability. However, as KGF alone did not produce any effect on P. aeruginosa , it seems to modulate the skin innate immune response.
Mariarosaria Galeano - One of the best experts on this subject based on the ideXlab platform.
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recombinant human erythropoietin improves angiogenesis and wound healing in Experimental Burn wounds
Critical Care Medicine, 2006Co-Authors: Mariarosaria Galeano, Domenica Altavilla, Alessandra Bitto, Letteria Minutoli, Margherita Calo, Patrizia Lo Cascio, Francesca Polito, Giovanni Giugliano, Giovanni Squadrito, Chiara MioniAbstract:Objective:Erythropoietin interacts with vascular endothelial growth factor (VEGF) and stimulates endothelial cell mitosis and motility; thus it may be of importance in the complex phenomenon of wound healing. The purpose of this study was to investigate the effect of recombinant human erythropoietin
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lipid peroxidation inhibition by raxofelast improves angiogenesis and wound healing in Experimental Burn wounds
Shock, 2005Co-Authors: Domenica Altavilla, Mariarosaria Galeano, Alessandra Bitto, Letteria Minutoli, Margherita Calo, Giovanni Squadrito, Paolo Seminara, F S Venuti, Michele R Colonna, Lo Cascio PAbstract:We investigated the effects of raxofelast, a lipid peroxidation inhibitor, in an Experimental model of Burn wounds. C57BL/6 male mice of 25-30 g were immersed in 80°C water for 10 seconds to achieve a partial-thickness scald Burn. Animals received intraperitoneally either raxofelast (20 mg/kg/day for 14 days in 100 μL) or its vehicle alone (100 μL/day for 14 days). On day 14, Burn areas were used for measuring conjugated dienes, reduced glutathione levels, histological damage, neoangiogenesis by immunohistochemistry and expression (Western blot) of the specific endothelial marker CD31 as well as quantification of microvessel density, VEGF wound content, endothelial and inducible nitric oxide synthase (eNOS and iNOS) expression and wound nitrite content. Raxofelast decreased tissue conjugated dienes (vehicle 6.1 ± 1.4 AABS/mg protein; raxofelast 3.7 ± 0.8 AABS/mg protein), prevented tissue glutathione consumption (vehicle 3.2 ± 0.9 μmol/g protein; raxofelast 6.7 ± 1.8 μmol/g protein), increased epithelial proliferation, extracellular matrix maturation, and augmented neoangiogenesis as suggested by the marked increase in microvessel density and by the robust expression of the specific endothelial marker CD31 (vehicle 9.4 ± 1.1 integrated intensity; raxofelast 14.8 ± 1.8 integrated intensity). Furthermore, raxofelast enhanced VEGF wound content (vehicle 1.4 ± 0.4 pg/mg protein; raxofelast 2.4 ± 0.6 pg/mg protein), caused a marked expression of eNOS (vehicle 16.1 ± 3 integrated intensity; raxofelast 26.2 ± 4 integrated intensity) and iNOS (vehicle 9.1 ± 1.8 integrated intensity; raxofelast 16.2 ± 3.5 integrated intensity) and increased wound nitrite content. Lipid peroxidation inhibition by raxofelast may be an effective therapeutic approach to improve clinical outcomes after thermal injury.
Domenica Altavilla - One of the best experts on this subject based on the ideXlab platform.
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recombinant human erythropoietin improves angiogenesis and wound healing in Experimental Burn wounds
Critical Care Medicine, 2006Co-Authors: Mariarosaria Galeano, Domenica Altavilla, Alessandra Bitto, Letteria Minutoli, Margherita Calo, Patrizia Lo Cascio, Francesca Polito, Giovanni Giugliano, Giovanni Squadrito, Chiara MioniAbstract:Objective:Erythropoietin interacts with vascular endothelial growth factor (VEGF) and stimulates endothelial cell mitosis and motility; thus it may be of importance in the complex phenomenon of wound healing. The purpose of this study was to investigate the effect of recombinant human erythropoietin
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lipid peroxidation inhibition by raxofelast improves angiogenesis and wound healing in Experimental Burn wounds
Shock, 2005Co-Authors: Domenica Altavilla, Mariarosaria Galeano, Alessandra Bitto, Letteria Minutoli, Margherita Calo, Giovanni Squadrito, Paolo Seminara, F S Venuti, Michele R Colonna, Lo Cascio PAbstract:We investigated the effects of raxofelast, a lipid peroxidation inhibitor, in an Experimental model of Burn wounds. C57BL/6 male mice of 25-30 g were immersed in 80°C water for 10 seconds to achieve a partial-thickness scald Burn. Animals received intraperitoneally either raxofelast (20 mg/kg/day for 14 days in 100 μL) or its vehicle alone (100 μL/day for 14 days). On day 14, Burn areas were used for measuring conjugated dienes, reduced glutathione levels, histological damage, neoangiogenesis by immunohistochemistry and expression (Western blot) of the specific endothelial marker CD31 as well as quantification of microvessel density, VEGF wound content, endothelial and inducible nitric oxide synthase (eNOS and iNOS) expression and wound nitrite content. Raxofelast decreased tissue conjugated dienes (vehicle 6.1 ± 1.4 AABS/mg protein; raxofelast 3.7 ± 0.8 AABS/mg protein), prevented tissue glutathione consumption (vehicle 3.2 ± 0.9 μmol/g protein; raxofelast 6.7 ± 1.8 μmol/g protein), increased epithelial proliferation, extracellular matrix maturation, and augmented neoangiogenesis as suggested by the marked increase in microvessel density and by the robust expression of the specific endothelial marker CD31 (vehicle 9.4 ± 1.1 integrated intensity; raxofelast 14.8 ± 1.8 integrated intensity). Furthermore, raxofelast enhanced VEGF wound content (vehicle 1.4 ± 0.4 pg/mg protein; raxofelast 2.4 ± 0.6 pg/mg protein), caused a marked expression of eNOS (vehicle 16.1 ± 3 integrated intensity; raxofelast 26.2 ± 4 integrated intensity) and iNOS (vehicle 9.1 ± 1.8 integrated intensity; raxofelast 16.2 ± 3.5 integrated intensity) and increased wound nitrite content. Lipid peroxidation inhibition by raxofelast may be an effective therapeutic approach to improve clinical outcomes after thermal injury.
Huseyin Karagoz - One of the best experts on this subject based on the ideXlab platform.
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Experimental stem cell therapies on Burn wound do source dose timing and method matter
Burns, 2015Co-Authors: Sinan Ozturk, Huseyin KaragozAbstract:Abstract Stem cell therapy has been introduced as a new and promising modality of wound covering in recent decade. It has been used for improvement of Burn wound, post Burn scar and saving stasis zone of Burn with good results. However, there have been some differences between the various Experimental Burn wound trials in stem cell source, therapeutic dose, delivery method and timing of stem cell delivery. In our study, we aimed to review stem cell biology and investigate discrepancies in animal trials of use of stem cells in Burn wound account for the variation in, stem cell source, therapeutic dose, delivery method and timing of stem cell delivery.
