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Xiangling You - One of the best experts on this subject based on the ideXlab platform.
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callose deposition is required for somatic embryogenesis in plasmolyzed eleutherococcus senticosus zygotic embryos
International Journal of Molecular Sciences, 2012Co-Authors: Lei Tao, Qiuyu Wang, Yang Yang, Xiangling YouAbstract:Dynamic changes in callose content, which is deposited as a plant defense response to physiological changes, were analyzed during somatic embryogenesis in Eleutherococcus senticosus zygotic embryos plasmolyzed in 1.0 M mannitol. During plasmolysis, callose deposition was clearly observed inside the plasma membrane of zygotic embryo epidermal cells using confocal laser scanning microscopy. The callose content of zygotic embryos gradually increased between 0 and 12 h plasmolysis and remained stable after 24 h plasmolysis. During eight weeks induction of somatic embryogenesis, the callose content of Explants plasmolyzed for 12 h was slightly higher than Explants plasmolyzed for 6 or 24 h, with the largest differences observed after 6 weeks culture, which coincided with the maximum callose content and highest number of globular somatic embryos. The highest frequency of somatic embryo formation was observed in Explants plasmolyzed for 12 h. The somatic embryo induction rate and number of somatic embryos per Explant were markedly different in zygotic embryos pretreated with plasmolysis alone (78.0%, 43 embryos per Explant) and those pretreated with plasmolysis and the callose synthase inhibitor 2-deoxy-d-glucose (11.5%, 8 embryos per Explant). This study indicates that callose production is required for somatic embryogenesis in plasmolyzed Explants.
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Callose Deposition Is Required for Somatic Embryogenesis in Plasmolyzed Eleutherococcus senticosus Zygotic Embryos
2012Co-Authors: Lei Tao, Qiuyu Wang, Yang Yang, Xiangling YouAbstract:Abstract: Dynamic changes in callose content, which is deposited as a plant defense response to physiological changes, were analyzed during somatic embryogenesis in Eleutherococcus senticosus zygotic embryos plasmolyzed in 1.0 M mannitol. During plasmolysis, callose deposition was clearly observed inside the plasma membrane of zygotic embryo epidermal cells using confocal laser scanning microscopy. The callose content of zygotic embryos gradually increased between 0 and 12 h plasmolysis and remained stable after 24 h plasmolysis. During eight weeks induction of somatic embryogenesis, the callose content of Explants plasmolyzed for 12 h was slightly higher than Explants plasmolyzed for 6 or 24 h, with the largest differences observed after 6 weeks culture, which coincided with the maximum callose content and highest number of globular somatic embryos. The highest frequency of somatic embryo formation was observed in Explants plasmolyzed for 12 h. The somatic embryo induction rate and number of somatic embryos per Explant were markedly different in zygotic embryos pretreated with plasmolysis alone (78.0%, 43 embryos per Explant) and those pretreated with plasmolysis and the callose synthase inhibitor 2-deoxy-D-glucose (11.5%, 8 embryos per Explant). This study indicates tha
Elena Corredoira - One of the best experts on this subject based on the ideXlab platform.
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propagation of mature quercus ilex l holm oak trees by somatic embryogenesis
Plant Cell Tissue and Organ Culture, 2017Co-Authors: M T Martinez, M San C Jose, A M Vieitez, M J Cernadas, Antonio Ballester, Elena CorredoiraAbstract:Somatic embryogenesis from in vitro leaf and shoot apex Explants excised from axillary shoot cultures established from two mature Quercus ilex trees has been developed. Somatic embryos (SE) were obtained from both Explant types and genotypes evaluated, although embryogenic frequencies were influenced by the genotype, auxin concentration, and Explant type. The Explants were cultured on Murashige and Skoog salts and vitamins, supplemented with 500 mg L−1 casein hydrolysate (CH) and different concentrations of indole-3-acetic acid or α-naphthalene acetic acid (NAA) in combination with 2.22 µM 6-benzylaminopurine (BA). In both genotypes, shoot apex Explants were more responsive than leaf Explants. The best results were obtained with apex Explants of clone Q3 (11%) cultured on medium with 21.48 µM NAA plus 2.22 µM BA. This combination was also effective for initiating SE from leaf Explants, although the induction rates were lower (1–3%). Embryogenic lines were maintained by repetitive embryogenesis following culture of nodular embryogenic structures on Schenk and Hildebrand medium without plant growth regulators. Low embryo multiplication rates were obtained when torpedo or early cotyledonary SE were used as initial Explant for embryo proliferation, or when glutamine or CH (500 mg L−1) was added to proliferation medium. For germination, cotyledonary-stage SE were isolated and stored at 4 °C for 2 months. After cold storage, SE were cultured on germination medium consisting of Gresshoff and Doy medium, supplemented with 0.44 μM BA and 20 μM silver thiosulphate. Under these conditions, plantlets were regenerated from 21 to 66.7% of the SE generated for both genotypes.
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thidiazuron induced high frequency plant regeneration from leaf Explants of paulownia tomentosa mature trees
Plant Cell Tissue and Organ Culture, 2008Co-Authors: Elena Corredoira, Antonio Ballester, A M VieitezAbstract:Attempts were made to study the effect of thidiazuron (TDZ) on adventitious shoot induction and plant development in Paulownia tomentosa Explants derived from mature trees. Media with different concentrations of TDZ in combination with an auxin were used to induce adventitious shoot-buds in two Explant types: basal leaf halves with the petiole attached (leaf Explant) and intact petioles. Optimal shoot regeneration was obtained in leaf Explants cultured on induction medium containing TDZ (22.7 or 27.3 μM) in combination with 2.9 μM indole-3-acetic acid (IAA) for 2 weeks, and subsequent culture in TDZ-free shoot development medium including 0.44 μM BA for a further 4-week period. The addition of IAA to the TDZ induction medium enhanced the shoot-forming capacity of Explants. The caulogenic response varied significantly with the position of the Explant along the shoot axis. The highest regeneration potential (85–87%) and shoot number (up to 17.6 shoots/Explant) were obtained in leaf Explants harvested from the most apical node exhibiting unfolded leaves (node 1). An analogous trend was also observed in intact petiole Explants, although shoot regeneration ability was considerably lower, with values ranging from 15% for petioles isolated from node 1 to 5% for those of nodes 2 and 3. Shoot formation capacity was influenced by the genotype, with regeneration frequencies ranging from 50% to 70%. It was possible to root elongated shoots (20 mm) in basal medium without growth regulators; however, rooting frequency was significantly increased up to 90% by a 7-day treatment with 0.5 μM indole-3-butyric acid, regardless of the previous culture period in shoot development medium (4 or 8 weeks). Shoot quality of rooted plantlets was improved not only by IBA treatment but also by using material derived from the 4-week culture period. Regenerated plantlets were successfully acclimatized in the greenhouse 8 weeks after transplanting.
A M Vieitez - One of the best experts on this subject based on the ideXlab platform.
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propagation of mature quercus ilex l holm oak trees by somatic embryogenesis
Plant Cell Tissue and Organ Culture, 2017Co-Authors: M T Martinez, M San C Jose, A M Vieitez, M J Cernadas, Antonio Ballester, Elena CorredoiraAbstract:Somatic embryogenesis from in vitro leaf and shoot apex Explants excised from axillary shoot cultures established from two mature Quercus ilex trees has been developed. Somatic embryos (SE) were obtained from both Explant types and genotypes evaluated, although embryogenic frequencies were influenced by the genotype, auxin concentration, and Explant type. The Explants were cultured on Murashige and Skoog salts and vitamins, supplemented with 500 mg L−1 casein hydrolysate (CH) and different concentrations of indole-3-acetic acid or α-naphthalene acetic acid (NAA) in combination with 2.22 µM 6-benzylaminopurine (BA). In both genotypes, shoot apex Explants were more responsive than leaf Explants. The best results were obtained with apex Explants of clone Q3 (11%) cultured on medium with 21.48 µM NAA plus 2.22 µM BA. This combination was also effective for initiating SE from leaf Explants, although the induction rates were lower (1–3%). Embryogenic lines were maintained by repetitive embryogenesis following culture of nodular embryogenic structures on Schenk and Hildebrand medium without plant growth regulators. Low embryo multiplication rates were obtained when torpedo or early cotyledonary SE were used as initial Explant for embryo proliferation, or when glutamine or CH (500 mg L−1) was added to proliferation medium. For germination, cotyledonary-stage SE were isolated and stored at 4 °C for 2 months. After cold storage, SE were cultured on germination medium consisting of Gresshoff and Doy medium, supplemented with 0.44 μM BA and 20 μM silver thiosulphate. Under these conditions, plantlets were regenerated from 21 to 66.7% of the SE generated for both genotypes.
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thidiazuron induced high frequency plant regeneration from leaf Explants of paulownia tomentosa mature trees
Plant Cell Tissue and Organ Culture, 2008Co-Authors: Elena Corredoira, Antonio Ballester, A M VieitezAbstract:Attempts were made to study the effect of thidiazuron (TDZ) on adventitious shoot induction and plant development in Paulownia tomentosa Explants derived from mature trees. Media with different concentrations of TDZ in combination with an auxin were used to induce adventitious shoot-buds in two Explant types: basal leaf halves with the petiole attached (leaf Explant) and intact petioles. Optimal shoot regeneration was obtained in leaf Explants cultured on induction medium containing TDZ (22.7 or 27.3 μM) in combination with 2.9 μM indole-3-acetic acid (IAA) for 2 weeks, and subsequent culture in TDZ-free shoot development medium including 0.44 μM BA for a further 4-week period. The addition of IAA to the TDZ induction medium enhanced the shoot-forming capacity of Explants. The caulogenic response varied significantly with the position of the Explant along the shoot axis. The highest regeneration potential (85–87%) and shoot number (up to 17.6 shoots/Explant) were obtained in leaf Explants harvested from the most apical node exhibiting unfolded leaves (node 1). An analogous trend was also observed in intact petiole Explants, although shoot regeneration ability was considerably lower, with values ranging from 15% for petioles isolated from node 1 to 5% for those of nodes 2 and 3. Shoot formation capacity was influenced by the genotype, with regeneration frequencies ranging from 50% to 70%. It was possible to root elongated shoots (20 mm) in basal medium without growth regulators; however, rooting frequency was significantly increased up to 90% by a 7-day treatment with 0.5 μM indole-3-butyric acid, regardless of the previous culture period in shoot development medium (4 or 8 weeks). Shoot quality of rooted plantlets was improved not only by IBA treatment but also by using material derived from the 4-week culture period. Regenerated plantlets were successfully acclimatized in the greenhouse 8 weeks after transplanting.
Santacruz-varela Amalio - One of the best experts on this subject based on the ideXlab platform.
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Inducción eficiente de brotes adventicios en cotiledones de Pinus maximartinezii Rzedowski
'Instituto de Ecologia A.C.', 2009Co-Authors: Robledo-paz Alejandrina, Santacruz-varela Amalio, Villalobos Arámbula, Víctor ManuelAbstract:Pinus maximartinezii Rzedowski is a nut pine native to Mexico that currently is considered as an endangered species because of disturbance of populations and natural habitat, as well as its limited geographic distribution. Even though such technologies as tissue culture could represent an alternative for its conservation and propagation, the existing literature for this pine is scarce. In this species is presented. Different types of Explants (embryos and cotyledons) were evaluated regarding their capacity to differentiate adventitious schoots. Explants from cotyledons showed the best response, so they were cultivated in a modified Shenk and Hildebrandt (SH) medium supplemented with four different concentratios (2.2 a 16.8 mg L-1) of N6-benzyladenine (BA). Percentages of Explants forming shoots and number of shoots developed per Explant were statistically different a mong the tested concentrations. The 16.8 mg L-1 of BA concentration induced the largest percentage of Explants forming shoots and number of shoots (53.5) and the highest number of shoots developed per Explant (29.1) after 14 weeks of culture. Three percent of the individual shoots produced roots. The developed methodology allows differentiation of adventitious shoots of Pinus maximartinezii from cotyledon Explants, with a multiplication rate significantly higher than the one obtained by other authors.Pinus maximartinezii Rzedowski es una especie de piñonero originaria de México, considerada en peligro de extinción debido a la alteración de sus poblaciones y de su hábitat, así como a su distribución geográfica retringida. A pesar de que tecnologías como el cultivo de tejidos podrían ser una alternativa para su conservación y propagación, la literatura existente al respecto es escasa. En este trabajo se presenta un protocolo que permite la diferenciación eficiente de brotes adventicios de este taxon. Se probaron embriones y cotiledones en cuanto a su capacidad para formar yemas adventicias. Los segundos mostraron la mejor respuesta y fueron entonces cultivados en el medio de Shenk y Hildebrandt (SH) modificado, suplementado con cuatro concentaciones (2.2 a 16.8 mg L-1) de deN6 -benciladenina (BA). El porcentaje de Explantes que produjeron brotes y el número de brotes formados en éstos fue estadísticamente diferente entre los niveles de BA probados; con 16.8 mg L-1 se indujo el mayor porcentaje de órganos que formaron brotes (53.5) y el número más alto de brotes generados por Explante (29.1) a las 14 semanas de cultivo de cotiledones, con una tasa de multiplicación siginificativamente superior a la obtenida por otros autores
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Inducción eficiente de brotes adventicios en cotiledones de Pinus maximartinezii Rzedowski
'Instituto de Ecologia A.C.', 2009Co-Authors: Robledo-paz Alejandrina, Villalobos Arámbula, Víctor Manuel, Santacruz-varela AmalioAbstract:Pinus maximartinezii Rzedowski is a nut pine native to Mexico that currently is considered as an endangered species because of disturbance of populations and natural habitat, as well as its limited geographic distribution. Even though such technologies as tissue culture could represent an alternative for its conservation and propagation, the existing literature for this pine is scarce. In this paper a protocol that allows an efficient differentiation of adventitious shoots for this species is presented. Different types of Explants (embryos and cotyledons) were evaluated regarding their capacity to differentiate adventitious shoots. Explants from cotyledons showed the best response, so they were cultivated in a modified Schenk and Hildebrandt (SH) medium supplemented with four different concentrations (2.2-16.8 mg L-1) of N6-benzyladenine (BA). Percentages of Explants forming shoots and number of shoots developed per Explant were statistically different among the tested concentrations. The 16.8 mg L-1 of BA concentration induced the largest percentage of Explants forming shoots (53.5) and the highest number of shoots developed per Explant (29.1) after 14 weeks of culture. Three percent of the individual shoots produced roots. The developed methodology allows differentiation of adventitious shoots of Pinus maximartinezii from cotyledon Explants, with a multiplication rate significantly higher than the one obtained by other authors.Pinus maximartinezii Rzedowski es una especie de piñonero originaria de México, considerada en peligro de extinción debido a la alteración de sus poblaciones y de su hábitat, así como a su distribución geográfica restringida. A pesar de que tecnologías como el cultivo de tejidos podrían ser una alternativa para su conservación y propagación, la literatura existente al respecto es escasa. En este trabajo se presenta un protocolo que permite la diferenciación eficiente de brotes adventicios de este taxon. Se probaron embriones y cotiledones en cuanto a su capacidad para formar yemas adventicias. Los segundos mostraron la mejor respuesta y fueron entonces cultivados en el medio de Schenk y Hildebrandt (SH) modificado, suplementado con cuatro concentraciones (2.2 a 16.8 mg L-1) deN6-benciladenina (BA). El porcentaje de Explantes que produjeron brotes y el número de brotes formados en éstos fue estadísticamente diferente entre los niveles de BA probados; con 16.8 mg L-1 se indujo el mayor porcentaje de órganos que formaron brotes (53.5) y el número más alto de brotes generados por Explante (29.1) a las 14 semanas de cultivo; de los vástagos individualizados, 3% formó raíces. La metodología desarrollada permite la diferenciación de yemas adventicias de P. maximartinezii a partir del cultivo de cotiledones, con una tasa de multiplicación significativamente superior a la obtenida por otros autores
Lei Tao - One of the best experts on this subject based on the ideXlab platform.
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callose deposition is required for somatic embryogenesis in plasmolyzed eleutherococcus senticosus zygotic embryos
International Journal of Molecular Sciences, 2012Co-Authors: Lei Tao, Qiuyu Wang, Yang Yang, Xiangling YouAbstract:Dynamic changes in callose content, which is deposited as a plant defense response to physiological changes, were analyzed during somatic embryogenesis in Eleutherococcus senticosus zygotic embryos plasmolyzed in 1.0 M mannitol. During plasmolysis, callose deposition was clearly observed inside the plasma membrane of zygotic embryo epidermal cells using confocal laser scanning microscopy. The callose content of zygotic embryos gradually increased between 0 and 12 h plasmolysis and remained stable after 24 h plasmolysis. During eight weeks induction of somatic embryogenesis, the callose content of Explants plasmolyzed for 12 h was slightly higher than Explants plasmolyzed for 6 or 24 h, with the largest differences observed after 6 weeks culture, which coincided with the maximum callose content and highest number of globular somatic embryos. The highest frequency of somatic embryo formation was observed in Explants plasmolyzed for 12 h. The somatic embryo induction rate and number of somatic embryos per Explant were markedly different in zygotic embryos pretreated with plasmolysis alone (78.0%, 43 embryos per Explant) and those pretreated with plasmolysis and the callose synthase inhibitor 2-deoxy-d-glucose (11.5%, 8 embryos per Explant). This study indicates that callose production is required for somatic embryogenesis in plasmolyzed Explants.
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Callose Deposition Is Required for Somatic Embryogenesis in Plasmolyzed Eleutherococcus senticosus Zygotic Embryos
2012Co-Authors: Lei Tao, Qiuyu Wang, Yang Yang, Xiangling YouAbstract:Abstract: Dynamic changes in callose content, which is deposited as a plant defense response to physiological changes, were analyzed during somatic embryogenesis in Eleutherococcus senticosus zygotic embryos plasmolyzed in 1.0 M mannitol. During plasmolysis, callose deposition was clearly observed inside the plasma membrane of zygotic embryo epidermal cells using confocal laser scanning microscopy. The callose content of zygotic embryos gradually increased between 0 and 12 h plasmolysis and remained stable after 24 h plasmolysis. During eight weeks induction of somatic embryogenesis, the callose content of Explants plasmolyzed for 12 h was slightly higher than Explants plasmolyzed for 6 or 24 h, with the largest differences observed after 6 weeks culture, which coincided with the maximum callose content and highest number of globular somatic embryos. The highest frequency of somatic embryo formation was observed in Explants plasmolyzed for 12 h. The somatic embryo induction rate and number of somatic embryos per Explant were markedly different in zygotic embryos pretreated with plasmolysis alone (78.0%, 43 embryos per Explant) and those pretreated with plasmolysis and the callose synthase inhibitor 2-deoxy-D-glucose (11.5%, 8 embryos per Explant). This study indicates tha
