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V J Andrade - One of the best experts on this subject based on the ideXlab platform.
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effects of the extender in the semen post thaw viability in young dairy gyr bulls bos taurus indicus pre selected by breeding soundness evaluation
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2011Co-Authors: A S Felipesilva, V Vale R Filho, M B D Ferreira, G S S Correa, M A Silva, Mirella M.s. Veras, V J AndradeAbstract:Semen cryopreservation from eight young dairy Gyr bulls was performed using two different semen Extenders. Bulls aging 25 months old and pre-selected for a high average score (84.4±5.6 in a 0-100 scale) in Zebu breeding soundness evaluation (BSE) composed the experimental group. Extenders were based on lactosis-egg yolk-glycerol and soya lecithin. Chilling and freezing curves were standardized by CRYOGEN® machine. Post-thaw features evaluated in semen frost in both Extenders - motility, vigor, major, minor and total deffects, morphological alteration in acrossome, bent tail, reative cells to hyposmotic sweeling test (Thos), and normal cells - were compared to the ones in the fresh ejaculate (except Thos) and among them. It was possible to freeze semen from all animals in the lactosis-egg yolk-glycerol extender. There were difference (P 80 points) is a good index to identify bulls with good post semen - thaw features. However, the choice of the extender is critical for obtaining acceptable results
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effects of the extender in the semen post thaw viability in young dairy gyr bulls bos taurus indicus pre selected by breeding soundness evaluation
Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2011Co-Authors: A S Felipesilva, V Vale R Filho, M B D Ferreira, G S S Correa, M A Silva, Mirella M.s. Veras, V J AndradeAbstract:Semen cryopreservation from eight young dairy Gyr bulls was performed using two different semen Extenders. Bulls aging 25 months old and pre-selected for a high average score (84.4±5.6 in a 0-100 scale) in Zebu breeding soundness evaluation (BSE) composed the experimental group. Extenders were based on lactosis-egg yolk-glycerol and soya lecithin. Chilling and freezing curves were standardized by CRYOGEN® machine. Post-thaw features evaluated in semen frost in both Extenders - motility, vigor, major, minor and total deffects, morphological alteration in acrossome, bent tail, reative cells to hyposmotic sweeling test (Thos), and normal cells - were compared to the ones in the fresh ejaculate (except Thos) and among them. It was possible to freeze semen from all animals in the lactosis-egg yolk-glycerol extender. There were difference (P 80 points) is a good index to identify bulls with good post semen - thaw features. However, the choice of the extender is critical for obtaining acceptable results
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effects of extender and equilibration time on post thaw motility and membrane integrity of cryopreserved gyr bull semen evaluated by casa and flow cytometry
Animal Reproduction Science, 2010Co-Authors: Ticiano Guimaraes Leite, Rubens Paes De Arruda, Vicente Ribeiro Do Vale Filho, Andre Furugen Cesar De Andrade, Lucas Luz Emerick, Fabiane Gilli Zaffalon, Jorge Andre Matias Martins, V J AndradeAbstract:The objectives of the present study were to investigate the effects of three equilibration times (0, 2, and 4 h) and two Extenders (TRIS or Bioxcell ® ) for cryopreservation of bull semen. Semen from 12 Gyr bulls was cryopreserved using an automated freezing machine. There were significant interactions between equilibration times and Extenders for sperm motility and membrane integrity. The control treatment (0 h equilibration) had the lowest values (P < 0.05) for total (MOT) and progressive motilities (PROG), and percentage of sperm with intact plasma and acrosomal membranes (IPIA), with no significant differences between Extenders. Extender TRIS had greater cryoprotective action than Bioxcell ® , with
Mahdi Zhandi - One of the best experts on this subject based on the ideXlab platform.
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flow cytometric and microscopic evaluation of post thawed ram semen cryopreserved in chemically defined home made or commercial Extenders
Animal Production Science, 2015Co-Authors: M Emamverdi, Mahdi Zhandi, Zare A Shahneh, Mohsen Sharafi, A Akhlaghi, Khodaei M Motlagh, F Dadkhah, Dadashpour N DavachiAbstract:The present study was designed to determine the effect of three different Extenders on ram sperm quality during a freeze–thawing procedure using flow cytometric and microscopic evaluations. Several in vitro qualitative analyses of post-thawed sperm parameters including motility and velocity parameters, plasma membrane functionality, total abnormality, capacitation status, acrosome integrity, mitochondrial activity and apoptosis features were considered. In the breeding season, seven ejaculates from each Zandi ram were collected routinely twice a week. Following semen collection, samples were pooled and equally divided into three aliquots. Each aliquot was diluted and frozen with one of the following Extenders: (1) Tris-based extender containing 1.5% (w/v) soybean lecithin (TSL), as a chemically defined extender, (2) Bioxcell, a commercial soybean lecithin-based extender, and (3) Tris-based extender containing 20% (v/v) egg yolk (TEY). The results of the present study indicated no differences in total [TSL (55.8 ± 2.02%) vs TEY (50.2 ± 2.02%; P < 0.05)] and progressive motility of spermatozoa [TSL (26.2 ± 1.36%) vs Bioxcell (22.4 ± 1.36%; P < 0.05)]. Semen freezing by means of TSL resulted in a higher percentage of live spermatozoa (39.42 ± 1.81%) compared with TEY (29.17 ± 1.81%; P < 0.05), and a higher percentage of functional plasma membrane (50.8 ± 192%) compared with TEY (44 ± 1.92%) and Bioxcell (38.8 ± 1.92%; P < 0.05). The effect of Extenders on sperm capacitation status showed that the percentage of post-thawed capacitated spermatozoa was higher in TEY (61.9 ± 1.48%) compared with that in TSL (56.6 ± 1.48%; P < 0.05). The evaluation of post-thawed spermatozoa indicated that the percentage of live spermatozoa with active mitochondria was higher in TSL (53.05 ± 2.31%) compared with Bioxcell (45.92 ± 2.31; P < 0.05) and the percentage of intact acrosome spermatozoa was higher in TSL (84.55 ± 2.51%) compared with TEY (74.91 ± 2.51%; P < 0.05). The use of TSL and Bioxcell Extenders reduced the percentage of apoptotic spermatozoa (40.82 ± 2.07% and 42.22 ± 2.07%, respectively), compared with TEY (51.34 ± 2.07%; P < 0.05). Post-thawing dead spermatozoa were increased when semen was frozen by Bioxcell (25.69 ± 1.28%). The results of this study showed that TSL extender may provide stabile milieu and conditions for ram sperm cryopreservation compared with Bioxcell and TEY Extenders. Whether TSL extender can improve the artificial insemination results remains, however, an open question.
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the effect of coenzyme q10 and α tocopherol in skim milk based extender for preservation of caspian stallion semen in cool condition
Journal of Equine Veterinary Science, 2014Co-Authors: Iman Yousefian, Ahmad Zareshahneh, Mahdi ZhandiAbstract:The purpose of this study was to determine the effects of different concentrations of coenzyme Q10 (CoQ10) and α-tocopherol (T) along with their interaction effects on the quality of preserved stallion semen at 5°C for a period of 48 hours. Semen was collected and diluted with skim milk–based extender that was supplemented with different antioxidants: no antioxidant (negative control [NC]), 0.9% (vol/vol) dimethyl sulfoxide (positive control [PC]), α-tocopherol (5 [T5] or 10 [T10] mM), CoQ10 (1 [C1] or 2 [C2] μM), 1 μM CoQ10 + 5 mM α-tocopherol (C1T5), 1 μM CoQ10 + 10 mM α-tocopherol (C1T10), 2 μM CoQ10 + 5 mM α-tocopherol (C2T5), and 2 μM CoQ10 + 10 mM α-tocopherol (C2T10), then kept at 5°C. The results showed that C1 extender resulted in higher total motility (62.44 ± 3.82) and plasma membrane integrity (65.16 ± 3.63%) compared with NC after 48 hours of storage (P .05). Also, C1T5 extender improved total and progressive motility, plasma membrane integrity and functionality, and decreased lipid peroxidation compared with NC and C2T10 Extenders over 48 hours of storage at 5°C (P < .05). The C1T5 extender was similar to C1 and T5 Extenders in all semen parameters evaluated during storage time. In conclusion, between previously mentioned Extenders, C1T5 could improve stallion sperm quality during 48 hours of storage. In the present study, none of Extenders had effect on sperm quality until 24-hour storage.
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optimization of ram semen cryopreservation using a chemically defined soybean lecithin based extender
Reproduction in Domestic Animals, 2013Co-Authors: M Emamverdi, Mahdi Zhandi, Zare A Shahneh, Mohsen Sharafi, Abbas AkbarisharifAbstract:Contents The purpose of the present study was to investigate the effects of a chemically defined soybean lecithin-based semen extender as a substitute for egg yolk-based Extenders in ram semen cryopreservation. In this study, 28 ejaculates were collected from four Zandi rams in the breeding season and then pooled together. The pooled semen was divided into six equal aliquots and diluted with six different Extenders: (i) Tris-based extender (TE) containing 0.5% (w/v) soybean lecithin (SL0.5), (ii) TE containing 1% (w/v) soybean lecithin (SL1), (iii) TE containing 1.5% (w/v) soybean lecithin (SL1.5), (iv) TE containing 2% (w/v) soybean lecithin (SL2), (v) TE containing 2.5% (w/v) soybean lecithin (SL2.5) and (vi) TE containing 20% (v/v) egg yolk (EYT). After thawing, sperm motility and motion parameters, plasma membrane and acrosome integrity, apoptosis status and mitochondrial activity were evaluated. The results shown that total and progressive motility (54.43 ± 1.33% and 25.43 ± 0.96%, respectively) were significantly higher in SL1.5 when compared to other semen Extenders. Sperm motion parameters (VAP, VSL, VCL, ALH and STR) were significantly higher in SL1.5 compared to other extender, with the exception of SL1 extender. Plasma membrane integrity (48.86 ± 1.38%) was significantly higher in SL1.5 when compared to other semen Extenders. Also, percentage of spermatozoa with intact acrosome in SL1.5 (85.35 ± 2.19%) extender was significantly higher than that in SL0.5, SL2.5 and EYT Extenders. The results showed that the proportion of live post-thawed sperm was significantly increased in SL1.5 extender compared to SL0.5, SL2 and EYT Extenders. In addition, SL1, SL1.5 and SL2.5 Extenders resulted in significantly lower percentage of early-apoptotic sperm than that in EYT extender. There were no significant differences in different semen Extenders for percentage of post-thawed necrotic and late-apoptotic spermatozoa. Also, the results indicated that there are slight differences for percentage of live spermatozoa with active mitochondria between Extenders. In conclusion, SL1.5 extender was better than other Extenders in most in vitro evaluated sperm parameters.
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does rosemary aqueous extract improve buck semen cryopreservation
Small Ruminant Research, 2013Co-Authors: Zaynab Zanganeh, Mahdi Zhandi, Ahmad Zareshahneh, Abozar Najafi, Mohammad Mahdi Nabi, Abdullah MohammadisangcheshmehAbstract:Abstract This study was conducted to investigate the effect of rosemary aqueous extract for protecting buck spermatozoa from freeze–thawing damages. In this study, a total of 32 ejaculates were collected from four Kordian bucks (8 ejaculates for each buck). On the day of semen collection, four ejaculates (one ejaculate for each buck) were pooled and diluted with egg yolk Tris-based Extenders containing 0 (E0), 2 (E2), 4 (E4), and 6 (E6) percent of rosemary aqueous extract. After thawing, sperm motility (computer-assisted sperm motility analysis (CASA)), membrane integrity (eosin/nigrosin) and functionality (Hypoosmotic swelling (HOS) test), abnormal morphology, lipid peroxidation (malondialdehyde (MDA) production), capacitation status (Chlortetracycline (CTC)), mitochondrial activity (rhodamine-123) and apoptotic features (Annexin V/propidium iodide) were assessed. The results showed that E4 extender significantly improved total (45.71 ± 1.49) and progressive (24.86 ± 0.84) motility, functional membrane (47.18 ± 1.37), and integrity (49.25 ± 1.17) of post-thawed buck spermatozoa when compared to E0 (36.43 ± 1.49, 18.43 ± 0.84, 39.42 ± 1.37 and 41.32 ± 1.17, respectively) and E6 (30.43 ± 1.49, 16.29 ± 0.84, 34.54 ± 1.37 and 36.39 ± 1.17, respectively) Extenders. Moreover, spermatozoa with abnormal morphology were significantly increased in E6 (22.5 ± 1.07) compared to E2 (17.29 ± 1.07) and E4 (16.11 ± 1.07) Extenders. The results showed that only E2 (1.69 ± 0.08) extender significantly decreased the level of MDA formation when compared to E0 (2.04 ± 0.08) and E4 (2.03 ± 0.08) extender. In this study, different level of rosemary aqueous extract failed to alter the CTC staining patterns and the mitochondrial activity of post-thawed buck spermatozoa. Moreover, E4 extender significantly increased live (55.92 ± 2.97) and decreased dead (22.64 ± 3.37) spermatozoa compared to other Extenders. In conclusion, addition of rosemary aqueous extract at level of 4% can improve post-thawed buck spermatozoa quality. More studies are required to reveal the active components in rosemary extract.
Boris Dzyuba - One of the best experts on this subject based on the ideXlab platform.
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DOI 10.1095/biolreprod.110.085852 Evaluating the Impacts of Osmotic and Oxidative Stress on Common Carp (Cyprinus carpio, L.) Sperm Caused by Cryopreservation Techniques1
2016Co-Authors: Boris Dzyuba, Martin Hulak, Marek Rodina, Otomar LinhartAbstract:Cryopreservation causes osmotic changes and oxidative damage that have sublethal and lethal effects on spermatozoa. We examined these osmotic and oxidative effects on common carp spermatozoa motility; membrane integrity; levels of thiobarbituric-acid-reactive substance (TBARS) and carbonyl groups (CP); and the activity of superoxide dismutase (SOD), glutathione reductase, and glutathione peroxidase (GPx). Sperm was diluted in dimethyl sulfoxide (DMSO) and ethylene glycol-based Extenders, followed by equilibration, freezing, and thawing. Equilibration in DMSO extender resulted in a significant reduction of spermatozoa motility, but motility was induced in those spermatozoa following dilution with saline buffer, which usually inhibits undiluted spermatozoa motility. Spermatozoa velocity and membrane integrity decreased with both Extenders following freezing and thawing. No significant difference in levels of TBARS or CP, or in SOD activity, was seen in samples equilibrated with either extender. The freeze/thaw process induced significantly higher levels of TBARS, CP, and GPx activity, but did not affect the level of SOD. Glutathione reductase activity was inhibited in samples exposed to DMSO extender. Ethylene glycol should be considered a preferred cryoprotective agent for common carp spermatozoa to reduce osmotic and oxidative stress during cryopreservation. antioxidant, cryopreservation, cryoprotectant, osmotic stress, sperm, spermatozoa motility, stres
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evaluating the impacts of osmotic and oxidative stress on common carp cyprinus carpio l sperm caused by cryopreservation techniques
Biology of Reproduction, 2010Co-Authors: Boris Dzyuba, Martin Hulak, Marek Rodina, Otomar LinhartAbstract:Cryopreservation causes osmotic changes and oxidative damage that have sublethal and lethal effects on spermatozoa. We examined these osmotic and oxidative effects on common carp spermatozoa motility; membrane integrity; levels of thiobarbituric-acid-reactive substance (TBARS) and carbonyl groups (CP); and the activity of superoxide dismutase (SOD), glutathione reductase, and glutathione peroxidase (GPx). Sperm was diluted in dimethyl sulfoxide (DMSO) and ethylene glycol-based Extenders, followed by equilibration, freezing, and thawing. Equilibration in DMSO extender resulted in a significant reduction of spermatozoa motility, but motility was induced in those spermatozoa following dilution with saline buffer, which usually inhibits undiluted spermatozoa motility. Spermatozoa velocity and membrane integrity decreased with both Extenders following freezing and thawing. No significant difference in levels of TBARS or CP, or in SOD activity, was seen in samples equilibrated with either extender. The freeze/thaw process induced significantly higher levels of TBARS, CP, and GPx activity, but did not affect the level of SOD. Glutathione reductase activity was inhibited in samples exposed to DMSO extender. Ethylene glycol should be considered a preferred cryoprotective agent for common carp spermatozoa to reduce osmotic and oxidative stress during cryopreservation.
M Emamverdi - One of the best experts on this subject based on the ideXlab platform.
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flow cytometric and microscopic evaluation of post thawed ram semen cryopreserved in chemically defined home made or commercial Extenders
Animal Production Science, 2015Co-Authors: M Emamverdi, Mahdi Zhandi, Zare A Shahneh, Mohsen Sharafi, A Akhlaghi, Khodaei M Motlagh, F Dadkhah, Dadashpour N DavachiAbstract:The present study was designed to determine the effect of three different Extenders on ram sperm quality during a freeze–thawing procedure using flow cytometric and microscopic evaluations. Several in vitro qualitative analyses of post-thawed sperm parameters including motility and velocity parameters, plasma membrane functionality, total abnormality, capacitation status, acrosome integrity, mitochondrial activity and apoptosis features were considered. In the breeding season, seven ejaculates from each Zandi ram were collected routinely twice a week. Following semen collection, samples were pooled and equally divided into three aliquots. Each aliquot was diluted and frozen with one of the following Extenders: (1) Tris-based extender containing 1.5% (w/v) soybean lecithin (TSL), as a chemically defined extender, (2) Bioxcell, a commercial soybean lecithin-based extender, and (3) Tris-based extender containing 20% (v/v) egg yolk (TEY). The results of the present study indicated no differences in total [TSL (55.8 ± 2.02%) vs TEY (50.2 ± 2.02%; P < 0.05)] and progressive motility of spermatozoa [TSL (26.2 ± 1.36%) vs Bioxcell (22.4 ± 1.36%; P < 0.05)]. Semen freezing by means of TSL resulted in a higher percentage of live spermatozoa (39.42 ± 1.81%) compared with TEY (29.17 ± 1.81%; P < 0.05), and a higher percentage of functional plasma membrane (50.8 ± 192%) compared with TEY (44 ± 1.92%) and Bioxcell (38.8 ± 1.92%; P < 0.05). The effect of Extenders on sperm capacitation status showed that the percentage of post-thawed capacitated spermatozoa was higher in TEY (61.9 ± 1.48%) compared with that in TSL (56.6 ± 1.48%; P < 0.05). The evaluation of post-thawed spermatozoa indicated that the percentage of live spermatozoa with active mitochondria was higher in TSL (53.05 ± 2.31%) compared with Bioxcell (45.92 ± 2.31; P < 0.05) and the percentage of intact acrosome spermatozoa was higher in TSL (84.55 ± 2.51%) compared with TEY (74.91 ± 2.51%; P < 0.05). The use of TSL and Bioxcell Extenders reduced the percentage of apoptotic spermatozoa (40.82 ± 2.07% and 42.22 ± 2.07%, respectively), compared with TEY (51.34 ± 2.07%; P < 0.05). Post-thawing dead spermatozoa were increased when semen was frozen by Bioxcell (25.69 ± 1.28%). The results of this study showed that TSL extender may provide stabile milieu and conditions for ram sperm cryopreservation compared with Bioxcell and TEY Extenders. Whether TSL extender can improve the artificial insemination results remains, however, an open question.
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optimization of ram semen cryopreservation using a chemically defined soybean lecithin based extender
Reproduction in Domestic Animals, 2013Co-Authors: M Emamverdi, Mahdi Zhandi, Zare A Shahneh, Mohsen Sharafi, Abbas AkbarisharifAbstract:Contents The purpose of the present study was to investigate the effects of a chemically defined soybean lecithin-based semen extender as a substitute for egg yolk-based Extenders in ram semen cryopreservation. In this study, 28 ejaculates were collected from four Zandi rams in the breeding season and then pooled together. The pooled semen was divided into six equal aliquots and diluted with six different Extenders: (i) Tris-based extender (TE) containing 0.5% (w/v) soybean lecithin (SL0.5), (ii) TE containing 1% (w/v) soybean lecithin (SL1), (iii) TE containing 1.5% (w/v) soybean lecithin (SL1.5), (iv) TE containing 2% (w/v) soybean lecithin (SL2), (v) TE containing 2.5% (w/v) soybean lecithin (SL2.5) and (vi) TE containing 20% (v/v) egg yolk (EYT). After thawing, sperm motility and motion parameters, plasma membrane and acrosome integrity, apoptosis status and mitochondrial activity were evaluated. The results shown that total and progressive motility (54.43 ± 1.33% and 25.43 ± 0.96%, respectively) were significantly higher in SL1.5 when compared to other semen Extenders. Sperm motion parameters (VAP, VSL, VCL, ALH and STR) were significantly higher in SL1.5 compared to other extender, with the exception of SL1 extender. Plasma membrane integrity (48.86 ± 1.38%) was significantly higher in SL1.5 when compared to other semen Extenders. Also, percentage of spermatozoa with intact acrosome in SL1.5 (85.35 ± 2.19%) extender was significantly higher than that in SL0.5, SL2.5 and EYT Extenders. The results showed that the proportion of live post-thawed sperm was significantly increased in SL1.5 extender compared to SL0.5, SL2 and EYT Extenders. In addition, SL1, SL1.5 and SL2.5 Extenders resulted in significantly lower percentage of early-apoptotic sperm than that in EYT extender. There were no significant differences in different semen Extenders for percentage of post-thawed necrotic and late-apoptotic spermatozoa. Also, the results indicated that there are slight differences for percentage of live spermatozoa with active mitochondria between Extenders. In conclusion, SL1.5 extender was better than other Extenders in most in vitro evaluated sperm parameters.
Dadashpour N Davachi - One of the best experts on this subject based on the ideXlab platform.
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flow cytometric and microscopic evaluation of post thawed ram semen cryopreserved in chemically defined home made or commercial Extenders
Animal Production Science, 2015Co-Authors: M Emamverdi, Mahdi Zhandi, Zare A Shahneh, Mohsen Sharafi, A Akhlaghi, Khodaei M Motlagh, F Dadkhah, Dadashpour N DavachiAbstract:The present study was designed to determine the effect of three different Extenders on ram sperm quality during a freeze–thawing procedure using flow cytometric and microscopic evaluations. Several in vitro qualitative analyses of post-thawed sperm parameters including motility and velocity parameters, plasma membrane functionality, total abnormality, capacitation status, acrosome integrity, mitochondrial activity and apoptosis features were considered. In the breeding season, seven ejaculates from each Zandi ram were collected routinely twice a week. Following semen collection, samples were pooled and equally divided into three aliquots. Each aliquot was diluted and frozen with one of the following Extenders: (1) Tris-based extender containing 1.5% (w/v) soybean lecithin (TSL), as a chemically defined extender, (2) Bioxcell, a commercial soybean lecithin-based extender, and (3) Tris-based extender containing 20% (v/v) egg yolk (TEY). The results of the present study indicated no differences in total [TSL (55.8 ± 2.02%) vs TEY (50.2 ± 2.02%; P < 0.05)] and progressive motility of spermatozoa [TSL (26.2 ± 1.36%) vs Bioxcell (22.4 ± 1.36%; P < 0.05)]. Semen freezing by means of TSL resulted in a higher percentage of live spermatozoa (39.42 ± 1.81%) compared with TEY (29.17 ± 1.81%; P < 0.05), and a higher percentage of functional plasma membrane (50.8 ± 192%) compared with TEY (44 ± 1.92%) and Bioxcell (38.8 ± 1.92%; P < 0.05). The effect of Extenders on sperm capacitation status showed that the percentage of post-thawed capacitated spermatozoa was higher in TEY (61.9 ± 1.48%) compared with that in TSL (56.6 ± 1.48%; P < 0.05). The evaluation of post-thawed spermatozoa indicated that the percentage of live spermatozoa with active mitochondria was higher in TSL (53.05 ± 2.31%) compared with Bioxcell (45.92 ± 2.31; P < 0.05) and the percentage of intact acrosome spermatozoa was higher in TSL (84.55 ± 2.51%) compared with TEY (74.91 ± 2.51%; P < 0.05). The use of TSL and Bioxcell Extenders reduced the percentage of apoptotic spermatozoa (40.82 ± 2.07% and 42.22 ± 2.07%, respectively), compared with TEY (51.34 ± 2.07%; P < 0.05). Post-thawing dead spermatozoa were increased when semen was frozen by Bioxcell (25.69 ± 1.28%). The results of this study showed that TSL extender may provide stabile milieu and conditions for ram sperm cryopreservation compared with Bioxcell and TEY Extenders. Whether TSL extender can improve the artificial insemination results remains, however, an open question.
