The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
Hiroshi Kitagaki - One of the best experts on this subject based on the ideXlab platform.
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residual mitochondrial transmembrane potential decreases unsaturated Fatty Acid Level in sake yeast during alcoholic fermentation
PeerJ, 2016Co-Authors: Kazutaka Sawada, Hiroshi KitagakiAbstract:Oxygen, a key nutrient in alcoholic fermentation, is rapidly depleted during this process. Several pathways of oxygen utilization have been reported in the yeast Saccharomyces cerevisiae during alcoholic fermentation, namely synthesis of unsaturated Fatty Acid, sterols and heme, and the mitochondrial electron transport chain. However, the interaction between these pathways has not been investigated. In this study, we showed that the major proportion of unsaturated Fatty Acids of ester-linked lipids in sake fermentation mash is derived from the sake yeast rather than from rice or koji (rice fermented with Aspergillus). Additionally, during alcoholic fermentation, inhibition of the residual mitochondrial activity of sake yeast increases the Levels of unsaturated Fatty Acids of ester-linked lipids. These findings indicate that the residual activity of the mitochondrial electron transport chain reduces molecular oxygen Levels and decreases the synthesis of unsaturated Fatty Acids, thereby increasing the synthesis of estery flavors by sake yeast. This is the first report of a novel link between residual mitochondrial transmembrane potential and the synthesis of unsaturated Fatty Acids by the brewery yeast during alcoholic fermentation.
Ökkeş Yilmaz - One of the best experts on this subject based on the ideXlab platform.
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A combination of alpha-tocopherol, vitamin C and N-acetyl cysteine increases unsaturated Fatty Acid Levels in hydrogen peroxide-induced Candida tropicalis (ATCC 13803).
Cell biochemistry and function, 2003Co-Authors: Ökkeş Yilmaz, Sait ÇelikAbstract:This research was aimed at evaluating the antioxidant effects of combinations of alpha lipoic Acid (LA), vitamin C (VC), N-acetyl cysteine (NAC) and alpha-tocopherol (TOC) on lipid Level and Fatty Acid composition of C. tropicalis (ATCC 13803) against hydrogen peroxide toxicity. According to the experimental results, the cell density of C. tmpicalis increased significantly in NAC + LA + H 2 O 2 , NAC + TOC + H 2 O 2 and NAC + VC + H 2 O 2 groups (p < 0.001) at the end of 48 and 72 h incubation times. The total lipid Level in H 2 O 2 and H 2 O 2 + antioxidant-supplemented groups was lower than that of the control group. In the Fatty Acid composition of C. tropicalis, the palmitic Acid Level was raised in the NAC group ( p < 0.05), whereas its Level was reduced in the other supplemented groups. While the oleic Acid Level increased in NAC + TOC + H 2 O 2 and NAC + VC + H 2 O 2 (p < 0.001) groups, its Level slightly decreased in the H 2 O 2 group. The linolenic Acid Level was low in all the supplemented groups, but linoleic Acid and total mono-unsaturated Fatty Acid (MUFA) Levels were high in these groups compared with the control group. Total polyunsaturated Fatty Acid Level (PUFA) decreased in NAC and H 2 O 2 groups (p < 0.01), but its Level increased in NAC + LA + H 2 O 2 and NAC + TOC + H 2 O 2 groups (respectively, p < 0.01, p < 0.001). Total saturated Fatty Acid Level decreased significantly in NAC + TOC + H 2 O 2 , NAC + H 2 O 2 and NAC + VC + H 2 O 2 (p < 0.001) groups (p < 0.01), whereas total unsaturated Fatty Acid Level increased in NAC, NAC + H 2 O 2 , NAC + LA + H 2 O 2 , NAC + TOC + H 2 O 2 and NAC + VC + H 2 O 2 groups. In conclusion, our data showed that the Levels of total unsaturated Fatty Acid, MUFA and PUFA were raised with the combinations of NAC and TOC, LA and VC in C. tropicalis cells subjected to hydrogen peroxide toxicity.
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effects of alpha lipoic Acid ascorbic Acid 6 palmitate and fish oil on the glutathione malonaldehyde and Fatty Acids Levels in erythrocytes of streptozotocin induced diabetic male rats
Journal of Cellular Biochemistry, 2002Co-Authors: Ökkeş Yilmaz, Yusuf Ozkan, Mehmet Yildirim, Ihsan A Ozturk, Yasemin ErsanAbstract:In this research, it has been aimed to evaluate the improvement effects of alpha lipoic Acid (ALA), ascorbic Acid-6-palmitate (AA6P), fish oil (FO), and their combination (COM) on some biochemical properties in erythrocytes of streptozotocin (STZ)-induced diabetic male rats. According to experimental results, glutathione (GSH) Level in erythrocytes decreased in diabetes (P < 0.01), D + ALA, and D + AA6P groups (P < 0.001). Malonaldehyde (MA) Level increased in diabetes (P < 0.05), D + FO, and D + COM groups (P < 0.001), but its Level in D + AA6P and D + ALA groups was lower in diabetes group (P < 0.01). Total lipid Level in diabetes and diabetes plus antioxidant administered groups were higher than control. Total cholesterol Level was high in diabetes and D + ALA groups (P < 0.05), but its Level reduced in D + FO compared to control and diabetes groups, P < 0.05, < 0.001, respectively. Total triglyceride (TTG) Level was high in the D + ALA (P < 0.05) and D + COM (P < 0.001) groups. In contrast, TTG Level in blood of diabetes group was higher than diabetes plus antioxidant and FO administered groups (P < 0.001). According to gas chromatography analysis results, while the palmitic Acid raised in diabetes group (P < 0.05), stearic Acid in D + FO, D + ALA, and diabetes groups was lower than control (P < 0.05), oleic Acid reduced in D + COM and D + FO groups, but its Level raised in D + AA6P and D + ALA groups (P < 0.01). As the linoleic Acid (LA) elevated in ALA + D, D + AA6P, and diabetes groups, linolenic Acid Level in diabetes, D + AA6P, and D + FO groups was lower than control (P < 0.001). Arachidonic Acid (AA) decreased in D + ALA, D+ AA6P, and diabetes groups (P < 0.01), but its Level in D + COM and D + FO was higher than control (P < 0.05). Docosahexaenoic Acid (DHA) increased in D + AA6P and D + COM (P < 0.05). While the total saturated Fatty Acid Level raised in diabetes group, its Level reduced in D + ALA and D + FO groups (P < 0.05). In contrast, total unsaturated Fatty Acid Level in D + ALA and D + FO groups was higher than control (P < 0.05). In conclusion, present data have confirmed that the combination of the ALA, AA6P, and FO have improvement effects on the recycling of GSSG to reduced GSH in erythrocytes of diabetic rats, and in addition to this, oxidative stress was suppressed by ALA and AA6P, and unsaturated Fatty Acid degree was raised by the effects of ALA and FO. J. Cell. Biochem. 86: 530–539, 2002. © 2002 Wiley-Liss, Inc.
Kazutaka Sawada - One of the best experts on this subject based on the ideXlab platform.
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residual mitochondrial transmembrane potential decreases unsaturated Fatty Acid Level in sake yeast during alcoholic fermentation
PeerJ, 2016Co-Authors: Kazutaka Sawada, Hiroshi KitagakiAbstract:Oxygen, a key nutrient in alcoholic fermentation, is rapidly depleted during this process. Several pathways of oxygen utilization have been reported in the yeast Saccharomyces cerevisiae during alcoholic fermentation, namely synthesis of unsaturated Fatty Acid, sterols and heme, and the mitochondrial electron transport chain. However, the interaction between these pathways has not been investigated. In this study, we showed that the major proportion of unsaturated Fatty Acids of ester-linked lipids in sake fermentation mash is derived from the sake yeast rather than from rice or koji (rice fermented with Aspergillus). Additionally, during alcoholic fermentation, inhibition of the residual mitochondrial activity of sake yeast increases the Levels of unsaturated Fatty Acids of ester-linked lipids. These findings indicate that the residual activity of the mitochondrial electron transport chain reduces molecular oxygen Levels and decreases the synthesis of unsaturated Fatty Acids, thereby increasing the synthesis of estery flavors by sake yeast. This is the first report of a novel link between residual mitochondrial transmembrane potential and the synthesis of unsaturated Fatty Acids by the brewery yeast during alcoholic fermentation.
Jian Shen - One of the best experts on this subject based on the ideXlab platform.
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additive effect of polymorphisms in the il 6 lta and tnf α genes and plasma Fatty Acid Level modulate risk for the metabolic syndrome and its components
The Journal of Clinical Endocrinology and Metabolism, 2010Co-Authors: Catherine M. Phillips, Louisa Goumidi, Sandrine Bertrais, Jane F. Ferguson, Martyn R. Field, Enda D. Kelly, John Mehegan, Gina M. Peloso, Adrienne L Cupples, Jian ShenAbstract:Context: Cytokine polymorphisms and dietary fat composition may influence the risk of the metabolic syndrome (MetS). Objective: The objective of the study was to determine the relationship between lymphotoxin-α (LTA), TNF-α, and IL-6 gene polymorphisms with MetS risk and investigate whether plasma Fatty Acid composition, a biomarker of dietary fat intake, modulated these associations. Design: Polymorphisms (LTA rs915654, TNF-α rs1800629, IL-6 rs1800797), biochemical measurements, and plasma Fatty Acids were determined in the LIPGENE-SU.VI.MAX study of MetS cases and matched controls (n = 1754). Results: LTA rs915654 minor A allele carriers and TNF-α rs1800629 major G allele homozygotes had increased MetS risk [odds ratio (OR) 1.37 (confidence interval [CI] 1.12–1.66), P = 0.002 and OR 1.35 (CI 1.08–1.70), P = 0.009] compared with their TT homozygotes and A allele carriers. Possession of the IL-6 rs1800797 GG genotype by the LTA and TNF-α risk genotype carriers further increased risk of the MetS [OR 2.10 (...
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Additive effect of polymorphisms in the IL-6, LTA, and TNF-{alpha} genes and plasma Fatty Acid Level modulate risk for the metabolic syndrome and its components
Journal of Clinical Endocrinology and Metabolism, 2010Co-Authors: Catherine M. Phillips, Louisa Goumidi, Sandrine Bertrais, Jane F. Ferguson, Martyn R. Field, Enda D. Kelly, John Mehegan, Gina M. Peloso, L. Adrienne Cupples, Jian ShenAbstract:Context: Cytokine polymorphisms and dietary fat composition may influence the risk of the metabolic syndrome (MetS). Objective: The objective of the study was to determine the relationship between lymphotoxin-α (LTA), TNF-α, and IL-6 gene polymorphisms with MetS risk and investigate whether plasma Fatty Acid composition, a biomarker of dietary fat intake, modulated these associations. Design: Polymorphisms (LTA rs915654, TNF-α rs1800629, IL-6 rs1800797), biochemical measurements, and plasma Fatty Acids were determined in the LIPGENE-SU.VI.MAX study of MetS cases and matched controls (n = 1754). Results: LTA rs915654 minor A allele carriers and TNF-α rs1800629 major G allele homozygotes had increased MetS risk [odds ratio (OR) 1.37 (confidence interval [CI] 1.12–1.66), P = 0.002 and OR 1.35 (CI 1.08–1.70), P = 0.009] compared with their TT homozygotes and A allele carriers. Possession of the IL-6 rs1800797 GG genotype by the LTA and TNF-α risk genotype carriers further increased risk of the MetS [OR 2.10 (CI 1.19–3.71) P = 0.009], fasting hyperglycemia [OR 2.65 (CI 1.12–6.28), P = 0.027], high systolic blood pressure [OR 1.99 (CI 1.07–3.72), P = 0.03], and abdominal obesity [OR 1.52 (CI 1.01–2.28), P = 0.04]. Plasma polyunsaturated to saturated fat ratio exacerbated these effects; subjects in the lowest 50th percentile had even greater risk of the MetS [OR 4.40 (CI 1.55–12.45), P = 0.005], fasting hyperglycemia, high systolic blood pressure, and abdominal obesity (P < 0.05). Conclusions: LTA, TNF-α, and IL-6 genotype interactions increased MetS risk, which was further exacerbated by a low plasma polyunsaturated to saturated fat exposure, indicating important modulation of genetic risk by dietary fat exposure
Woo Keun Seo - One of the best experts on this subject based on the ideXlab platform.
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high free Fatty Acid Level is associated with recurrent stroke in cardioembolic stroke patients
Neurology, 2014Co-Authors: Jeong Yoon Choi, Ji Sun Kim, Jihyun Kim, Seong Beom Koh, Woo Keun SeoAbstract:Objective: To determine whether the plasma Level of free Fatty Acid (FFA) could be associated with recurrent stroke in cardioembolic (CE) stroke patients. Methods: We analyzed data from 669 acute ischemic stroke patients and examined the association between FFA concentration and recurrent stroke in CE stroke patients compared with non-CE stroke patients. Results: The baseline plasma FFA concentration (mEq/L) was approximately 1.5-fold higher in CE stroke patients (1.01 ± 0.63) than in non-CE stroke patients (0.72 ± 0.51). Multivariate logistic analysis showed that an increased Level of FFA was significantly associated with CE stroke (hazard ratio [HR] 2.124, confidence interval [CI] 1.492–3.024). During the mean follow-up period of 25.4 months, a total of 56 (8.4%) patients experienced a stroke recurrence. The recurrence rate did not differ between patients with CE (10.5%) and non-CE (8.0%) stroke ( p = 0.396). In CE stroke patients, an elevated baseline FFA concentration was independently associated with stroke recurrence (HR 2.711, CI 1.056–6.959). However, there was no association between FFA and stroke recurrence in non-CE stroke patients. Conclusion: In this retrospective registry-based observational study, CE stroke seemed to be associated with elevated plasma Level of FFA. In addition, the present study suggested that an elevated FFA concentration could be a useful indicator for predicting recurrent stroke in CE stroke patients.
