The Experts below are selected from a list of 25098 Experts worldwide ranked by ideXlab platform
Thomas A. Hinners - One of the best experts on this subject based on the ideXlab platform.
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Speciation of Arsenic Animal Feed Additives by Microbore High-performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
Analyst, 1997Co-Authors: Spiros A. Pergantis, E. Heithmar, Thomas A. HinnersAbstract:Phenylarsonic compounds have been used as poultry and swine Feed Additives for the purpose of growth promotion and disease prevention. Owing to the lack of suitable analytical methods, however, knowledge of their metabolism, environmental fate and impact remains incomplete. In order to compensate for this, analytical procedures were developed that allow the speciation of arsenic animal Feed Additives by using microbore high-performance liquid chromatography (µHPLC) coupled on-line with ICP-MS. More specifically, reversed-phase (RP) chromatographic methods were optimised to achieve the separation of various phenylarsonic acids from each other and from the more toxic inorganic arsenic compounds. This mode of chromatography, however, exhibits limitations, especially in the presence of naturally occurring organoarsenic compounds. The application of RP ion-pairing chromatography eliminates such shortcomings by minimising the co-elution of arsenic species. In general, the µHPLC–ICP-MS methods developed in this study provide high selectivity, extremely good sensitivity, low limits of detection (low-ppb or sub-pg amounts of As), require small sample volumes (
Spiros A. Pergantis - One of the best experts on this subject based on the ideXlab platform.
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Speciation of Arsenic Animal Feed Additives by Microbore High-performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
Analyst, 1997Co-Authors: Spiros A. Pergantis, E. Heithmar, Thomas A. HinnersAbstract:Phenylarsonic compounds have been used as poultry and swine Feed Additives for the purpose of growth promotion and disease prevention. Owing to the lack of suitable analytical methods, however, knowledge of their metabolism, environmental fate and impact remains incomplete. In order to compensate for this, analytical procedures were developed that allow the speciation of arsenic animal Feed Additives by using microbore high-performance liquid chromatography (µHPLC) coupled on-line with ICP-MS. More specifically, reversed-phase (RP) chromatographic methods were optimised to achieve the separation of various phenylarsonic acids from each other and from the more toxic inorganic arsenic compounds. This mode of chromatography, however, exhibits limitations, especially in the presence of naturally occurring organoarsenic compounds. The application of RP ion-pairing chromatography eliminates such shortcomings by minimising the co-elution of arsenic species. In general, the µHPLC–ICP-MS methods developed in this study provide high selectivity, extremely good sensitivity, low limits of detection (low-ppb or sub-pg amounts of As), require small sample volumes (
Yeon Ho Je - One of the best experts on this subject based on the ideXlab platform.
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transformation of beauveria bassiana to produce egfp in tenebrio molitor for use as animal Feed Additives
Fems Microbiology Letters, 2013Co-Authors: Jae Su Kim, Zhenli Fu, Se-jin Lee, Ju Hyun Lee, Bruce L. Parker, Margaret Skinner, Jaeyoung Choi, Yeon Ho JeAbstract:Efforts are underway to develop more effective and safer animal Feed Additives. Entomopathogenic fungi can be considered practical expression platforms of functional genes within insects which have been used as animal Feed Additives. In this work, as a model, the enhanced green fluorescent protein (egfp) gene was expressed in yellow mealworms, Tenebrio molitor by highly infective Beauveria bassiana ERL1170. Among seven test isolates, ERL1170 treatment showed 57.1% and 98.3% mortality of mealworms 2 and 5 days after infection, respectively. The fungal transformation vector, pABeG containing the egfp gene, was inserted into the genomic DNA of ERL1170 using the restriction enzyme-mediated integration method. This resulted in the generation of the transformant, Bb-egfp#3, which showed the highest level of fluorescence. Bb-egfp#3-treated mealworms gradually turned dark brown, and in 7-days mealworm sections showed a strong fluorescence. This did not occur in the wild-type strain. This work suggests that further valuable proteins can be efficiently produced in this mealworm-based fungal expression platform, thereby increasing the value of mealworms in the animal Feed additive industry.
Olurotimi A. Olafadehan - One of the best experts on this subject based on the ideXlab platform.
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Essential oils and phytogenic Feed Additives in ruminant diet: chemistry, ruminal microbiota and fermentation, Feed utilization and productive performance
Phytochemistry Reviews, 2021Co-Authors: Ahmed E. Kholif, Olurotimi A. OlafadehanAbstract:A systematic approach was used to review, elucidate and summarize the importance of phytochemicals and essential oils (EO) as substitutes for chemical Feed Additives in ruminant Feeding. Using PRISMA statement for defined search strategy, screening, selection and obtaining reliable conclusions, only articles that met the predefined selection criteria were used for the review. Phytochemicals Feed Additives (PFA) contain EO and a lot of other plant secondary metabolites (PSM). Essential oils are volatile aromatic compounds in most cases, and they compose of terpenoids and phenylpropanoids, which have antimicrobial properties. They have been the subject of persistent consideration of animal nutritionists who have been evaluating their use as Feed Additives to enhance ruminal fermentation and Feed utilization efficiency. As alternatives to non-nutritive chemical and antibiotic Feed Additives, many phytochemicals (i.e. PSM), from a broad spectrum of herbs and spices, have been used to modify ruminal fermentation characteristics, inhibit ruminal methanogenesis and enhance livestock performance. The main limitations of PFA are defining the optimal doses that enhance animal performance and the adaptation of ruminal microflora to their presence in diets, resulting in inconsistent and inconclusive results. Many factors affecting the effective dose of phytochemicals include the chemical structures of phytochemical supplements/Additives, the animal diet and animal physiological status. This review illustrates the possibility of using PFA on ruminal microbiota and their adaptation to phytochemicals, ruminal fermentation and animal performance.
E. Heithmar - One of the best experts on this subject based on the ideXlab platform.
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Speciation of Arsenic Animal Feed Additives by Microbore High-performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
Analyst, 1997Co-Authors: Spiros A. Pergantis, E. Heithmar, Thomas A. HinnersAbstract:Phenylarsonic compounds have been used as poultry and swine Feed Additives for the purpose of growth promotion and disease prevention. Owing to the lack of suitable analytical methods, however, knowledge of their metabolism, environmental fate and impact remains incomplete. In order to compensate for this, analytical procedures were developed that allow the speciation of arsenic animal Feed Additives by using microbore high-performance liquid chromatography (µHPLC) coupled on-line with ICP-MS. More specifically, reversed-phase (RP) chromatographic methods were optimised to achieve the separation of various phenylarsonic acids from each other and from the more toxic inorganic arsenic compounds. This mode of chromatography, however, exhibits limitations, especially in the presence of naturally occurring organoarsenic compounds. The application of RP ion-pairing chromatography eliminates such shortcomings by minimising the co-elution of arsenic species. In general, the µHPLC–ICP-MS methods developed in this study provide high selectivity, extremely good sensitivity, low limits of detection (low-ppb or sub-pg amounts of As), require small sample volumes (