The Experts below are selected from a list of 249 Experts worldwide ranked by ideXlab platform
Jill Cook - One of the best experts on this subject based on the ideXlab platform.
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Reduced Levels of Aligned Fibrillar Structure Are Not Associated With Achilles and Patellar Tendon Symptoms.
Clinical journal of sport medicine : official journal of the Canadian Academy of Sport Medicine, 2020Co-Authors: Sea Docking, Jill Cook, Michael Girdwood, Lauren V. Fortington, Ebonie RioAbstract:OBJECTIVE To investigate whether the mean cross-sectional area (mCSA) of aligned Fibrillar Structure (AFS) was associated with the presence and severity of symptoms. DESIGN Prospective cohort study. PARTICIPANTS One hundred seventy-five elite male Australian football players completed monthly Oslo Sports Trauma Research Center overuse injury questionnaires for both the Achilles and patellar tendon over the season to ascertain the presence and severity of symptoms. At the start of the preseason, participants underwent ultrasound tissue characterization (UTC) imaging of the Achilles and patellar tendon. MAIN OUTCOME MEASURES Images were classified as normal or abnormal based on gray-scale ultrasound. Based on UTC quantification, the mCSA of AFS was compared between those with and without current symptoms. RESULTS No difference in the mCSA of AFS was observed between those with or without tendon symptoms (P < 0.05). Similar to previous findings, 80% to 92% of abnormal tendons had similar amounts of mCSA of AFS compared with normal tendon. If reduced mCSA of AFS was present, it was not associated with the presence or severity of symptoms. CONCLUSIONS The prevalence, development, or severity of symptoms was not associated with decreased levels of AFS in the Achilles or patellar tendon. This suggests that a lack of structural integrity is not linked to symptoms and questions the rationale behind regenerative medicine. Most tendons are able to compensate for areas of disorganization and maintain tissue homeostasis.
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pathological tendons maintain sufficient aligned Fibrillar Structure on ultrasound tissue characterization utc
Scandinavian Journal of Medicine & Science in Sports, 2016Co-Authors: Sea Docking, Jill CookAbstract:Structural disorganization in the tendon is associated with tendinopathy, with little research investigating whether disorganization overwhelms the overall structural integrity of the tendon. This study investigated the mean cross-sectional area (CSA) of aligned Fibrillar Structure as detected by ultrasound tissue characterization (UTC) in the pathological and normal Achilles and patellar tendons. Ninety-one participants had their Achilles and/or patellar tendons scanned using UTC to capture a three-dimensional image of the tendon and allow a semi-quantification of the echopattern. The mean CSA of aligned Fibrillar Structure (echo type I + II) and disorganized Structure (echo type III + IV) was calculated based on UTC algorithms. Each tendon was classified as either pathological or normal based solely on gray-scale ultrasound. The mean CSA of aligned Fibrillar Structure was significantly greater (P ≤ 0.001) in the pathological tendon compared with the normal tendon, despite the pathological tendon containing greater amounts of disorganized Structure (P ≤ 0.001). A significant relationship was observed between the mean CSA of disorganized Structure and anteroposterior diameter of the Achilles (R(2) = 0.587) and patellar (R(2) = 0.559) tendons. This study is the first to show that pathological tendons have sufficient levels of aligned Fibrillar Structure. Pathological tendons may compensate for areas of disorganization by increasing in tendon thickness.
T. Komatsu - One of the best experts on this subject based on the ideXlab platform.
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Fibrillar Structure of superdrawn polyoxymethylene fibres
Journal of Materials Science, 1993Co-Authors: T. KomatsuAbstract:A fibre Structure of superdrawn polyoxymethylene fibres was examined by scanning electron microscopy. The fibre had a skin-core Structure and was characterized by a double Fibrillar network which was formed from a frame network of trunk fibril screens parallel to the fibre axis, a sub-network of branch fibrils inside the frame, thin cross-fibrils connected to the network and longitudinal void-chains connected to the cross-fibrils between the fibril screens. The frame Fibrillar network originated in the undrawn spherulite network, trunk and branch fibrils originated in a bundle of lamellar crystallites and cross-fibrils probably resulted from the interlamellar amorphous phase. The Fibrillar network was also varied in the radius direction, possibly due to the diversities of the undrawn spherulitic morphology and the deformation mode at necking.
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Fracture process of superdrawn polyoxymethylene fibres
Journal of Materials Science, 1993Co-Authors: T. KomatsuAbstract:A fracture process of superdrawn polyoxymethylene fibres was studied in terms of the structural deformation under stress by scanning electron microscopy. The Fibrillar Structure consisted of two structural units showing different stress-fracture Behaviour, I.e. one was a linear void-chain connected to thin cross-fibrils which was fragile to tensile stress; the other was a network of thick fibrils which was very resistant to stress and prevent lateral development of void-fracture. Fracture began from a dominant fracture of void-chains followed by a Fibrillar fracture at the Micronecks, i.e. a shear band fracture. This process was clearly due to the above Fibrillar Structure including voids. Void-fracture hardly influenced Fibrillar fracture, suggesting a possible dependence of the dimensions of the frame fibrils on fracture strength. Also, the fracture process clearly included Fibrillar slippage and heat generation.
Sea Docking - One of the best experts on this subject based on the ideXlab platform.
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Reduced Levels of Aligned Fibrillar Structure Are Not Associated With Achilles and Patellar Tendon Symptoms.
Clinical journal of sport medicine : official journal of the Canadian Academy of Sport Medicine, 2020Co-Authors: Sea Docking, Jill Cook, Michael Girdwood, Lauren V. Fortington, Ebonie RioAbstract:OBJECTIVE To investigate whether the mean cross-sectional area (mCSA) of aligned Fibrillar Structure (AFS) was associated with the presence and severity of symptoms. DESIGN Prospective cohort study. PARTICIPANTS One hundred seventy-five elite male Australian football players completed monthly Oslo Sports Trauma Research Center overuse injury questionnaires for both the Achilles and patellar tendon over the season to ascertain the presence and severity of symptoms. At the start of the preseason, participants underwent ultrasound tissue characterization (UTC) imaging of the Achilles and patellar tendon. MAIN OUTCOME MEASURES Images were classified as normal or abnormal based on gray-scale ultrasound. Based on UTC quantification, the mCSA of AFS was compared between those with and without current symptoms. RESULTS No difference in the mCSA of AFS was observed between those with or without tendon symptoms (P < 0.05). Similar to previous findings, 80% to 92% of abnormal tendons had similar amounts of mCSA of AFS compared with normal tendon. If reduced mCSA of AFS was present, it was not associated with the presence or severity of symptoms. CONCLUSIONS The prevalence, development, or severity of symptoms was not associated with decreased levels of AFS in the Achilles or patellar tendon. This suggests that a lack of structural integrity is not linked to symptoms and questions the rationale behind regenerative medicine. Most tendons are able to compensate for areas of disorganization and maintain tissue homeostasis.
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pathological tendons maintain sufficient aligned Fibrillar Structure on ultrasound tissue characterization utc
Scandinavian Journal of Medicine & Science in Sports, 2016Co-Authors: Sea Docking, Jill CookAbstract:Structural disorganization in the tendon is associated with tendinopathy, with little research investigating whether disorganization overwhelms the overall structural integrity of the tendon. This study investigated the mean cross-sectional area (CSA) of aligned Fibrillar Structure as detected by ultrasound tissue characterization (UTC) in the pathological and normal Achilles and patellar tendons. Ninety-one participants had their Achilles and/or patellar tendons scanned using UTC to capture a three-dimensional image of the tendon and allow a semi-quantification of the echopattern. The mean CSA of aligned Fibrillar Structure (echo type I + II) and disorganized Structure (echo type III + IV) was calculated based on UTC algorithms. Each tendon was classified as either pathological or normal based solely on gray-scale ultrasound. The mean CSA of aligned Fibrillar Structure was significantly greater (P ≤ 0.001) in the pathological tendon compared with the normal tendon, despite the pathological tendon containing greater amounts of disorganized Structure (P ≤ 0.001). A significant relationship was observed between the mean CSA of disorganized Structure and anteroposterior diameter of the Achilles (R(2) = 0.587) and patellar (R(2) = 0.559) tendons. This study is the first to show that pathological tendons have sufficient levels of aligned Fibrillar Structure. Pathological tendons may compensate for areas of disorganization by increasing in tendon thickness.
Jinxi Wang - One of the best experts on this subject based on the ideXlab platform.
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role of Fibrillar Structure of collagenous carrier in bone sialoprotein mediated matrix mineralization and osteoblast differentiation
Biomaterials, 2007Co-Authors: Andy L. Anderson, Jinxi WangAbstract:To investigate the effects of the microStructure of collagenous carriers on the in vivo function of bone sialoprotein (BSP) in mineralization and osteoblast differentiation, we examined the ultraStructure of reconstituted type I collagen (collagen) and heat-denatured collagen (gelatin) and the in vivo responses to purified bone-derived BSP that was implanted with collagen or gelatin into surgically created 8-mm rat calvarial bone defects. Scanning and transmission electron microscopies revealed that the collagen displayed a fine Fibrillar Structure with interconnecting spaces between the fibrils/fibers, while the gelatin completely lost this unique three-dimensional Structure after denaturation. The rates of in vivo release of BSP from the collagen scaffold were significantly lower than those from the gelatin. Collagen-BSP, but not gelatin-BSP, induced early mineral deposition in the matrix of proliferating repair cells in the calvarial defects at ∼4–7 days after implantation. Expression levels of osteoblast markers, alkaline phosphatase activity and amounts of new bone synthesized in the collagen-BSP treated defects were significantly greater than that in the gelatin-BSP treated defects (p<0.001). The data suggest that the Fibrillar microStructure of reconstituted collagen is essential for retaining BSP at a higher concentration within the defects, which enhances BSP-mediated matrix mineralization and osteoblast differentiation during the repair of rat calvarial defects.
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Role of Fibrillar Structure of collagenous carrier in bone sialoprotein-mediated matrix mineralization and osteoblast differentiation.
Biomaterials, 2006Co-Authors: Andy L. Anderson, Jinxi WangAbstract:To investigate the effects of the microStructure of collagenous carriers on the in vivo function of bone sialoprotein (BSP) in mineralization and osteoblast differentiation, we examined the ultraStructure of reconstituted type I collagen (collagen) and heat-denatured collagen (gelatin) and the in vivo responses to purified bone-derived BSP that was implanted with collagen or gelatin into surgically created 8-mm rat calvarial bone defects. Scanning and transmission electron microscopies revealed that the collagen displayed a fine Fibrillar Structure with interconnecting spaces between the fibrils/fibers, while the gelatin completely lost this unique three-dimensional Structure after denaturation. The rates of in vivo release of BSP from the collagen scaffold were significantly lower than those from the gelatin. Collagen-BSP, but not gelatin-BSP, induced early mineral deposition in the matrix of proliferating repair cells in the calvarial defects at ∼4–7 days after implantation. Expression levels of osteoblast markers, alkaline phosphatase activity and amounts of new bone synthesized in the collagen-BSP treated defects were significantly greater than that in the gelatin-BSP treated defects (p
Paul J Campagnola - One of the best experts on this subject based on the ideXlab platform.
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second harmonic generation microscopy for quantitative analysis of collagen Fibrillar Structure
Nature Protocols, 2012Co-Authors: Xiyi Chen, Oleg Nadiarynkh, Sergey V Plotnikov, Paul J CampagnolaAbstract:Second harmonic generation microscopy for quantitative analysis of collagen Fibrillar Structure
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Second harmonic generation microscopy for quantitative analysis of collagen Fibrillar Structure
Nature Protocols, 2012Co-Authors: Xiyi Chen, Oleg Nadiarynkh, Sergey Plotnikov, Paul J CampagnolaAbstract:Second-harmonic generation (SHG) microscopy has emerged as a powerful modality for imaging Fibrillar collagen in a diverse range of tissues. Because of its underlying physical origin, it is highly sensitive to the collagen fibril/fiber Structure, and, importantly, to changes that occur in diseases such as cancer, fibrosis and connective tissue disorders. We discuss how SHG can be used to obtain more structural information on the assembly of collagen in tissues than is possible by other microscopy techniques. We first provide an overview of the state of the art and the physical background of SHG microscopy, and then describe the optical modifications that need to be made to a laser-scanning microscope to enable the measurements. Crucial aspects for biomedical applications are the capabilities and limitations of the different experimental configurations. We estimate that the setup and calibration of the SHG instrument from its component parts will require 2–4 weeks, depending on the level of the user′s experience.