The Experts below are selected from a list of 282 Experts worldwide ranked by ideXlab platform

Akira Tsuji - One of the best experts on this subject based on the ideXlab platform.

  • tissue selective drug delivery utilizing carrier mediated transport systems
    Journal of Controlled Release, 1999
    Co-Authors: Akira Tsuji
    Abstract:

    This paper describes some successful examples of a tissue selective drug delivery by utilizing specialized transporter(s) expressed in the targeted tissue Cells. These are as follows: (1) oral delivery via H + /oligopeptide transporter, rat or human Peptl, in the intestine for β-lactam antibiotics and a newly synthesized dipeptide, L-dopa-L-phenylalanine; (2) tumor Cell specific delivery via the newly discovered H + /oligopeptide transporter(s) expressed in human Fibrosarcoma Cell Line HT-1080 for model oligopeptides, glycylsarcosine and carnosine; (3) oral and hepatic delivery via an H + /monocarboxylate transporter in the intestine and an organic anion transporter in the liver for HMG-CoA reductase inhibitor, pravastatin; and (4) lung selective delivery via some type of transporter and avoidance of transfer into the brain via P-glycoprotein at the blood-brain barrier for a new quinolone antibacterial, HSR-903.

  • carrier mediated transport of oligopeptides in the human Fibrosarcoma Cell Line ht1080
    Cancer Research, 1997
    Co-Authors: Takeo Nakanishi, Takuma Sasaki, Ikumi Tamai, Akira Tsuji
    Abstract:

    To explore the feasibility of targeting human tumor Cells via their transport systems, dipeptide uptake was studied in the human Fibrosarcoma Cell Line HT1080 and the human fibroblast Cell Line IMR-90 by the use of hydrolysis-resistant glycylsarcosine (Gly-Sar). The uptake of [14C]Gly-Sar into HT1080 was time dependent. Kinetic analysis of the concentration dependence of the initial rate of [14C]Gly-Sar uptake showed that a carrier-mediated transport system with a K m of 11.4 ± 3.3 mm and V max of 26.8 ± 4.0 (nmol/15 min/mg protein) and a nonsaturable component ( k d of 0.80 µl/15 min/mg protein) were responsible for the dipeptide uptake by HT1080 Cells. The optimal pH for the maximal uptake was around 6.0. [14C]Gly-Sar uptake was inhibited by various di- and tripeptides and peptide-mimetic drugs, such as bestatin and cefadroxil. [14C]Gly-Sar uptake was not affected by the presence of amino acids or tetra- or pentapeptides. The uptake of cefadroxil was reduced significantly by unlabeled Gly-Sar. Moreover, Gly-Gly and Gly-Leu produced an increase in the apparent K m of the uptake of Gly-Sar without altering V max. On the other hand, dipeptide uptake by IMR-90, which is a normal diploid Cell Line (not malignant), showed no saturable transport. These results suggest that HT1080 Cells take up dipeptides via a pH-dependent transporter. This is the first report showing that a dipeptide transport system, which is similar but not identical to the well-characterized oligopeptide transporters PepT1 and PepT2, exists in fibroblast-derived tumor Cells but not in normal fibroblasts. The present finding could be the basis of a novel strategy for the specific delivery of oligopeptide-mimetic anticancer drugs into tumor Cells.

Altomare Di Benedetto - One of the best experts on this subject based on the ideXlab platform.

  • targeting a newly established spontaneous feLine Fibrosarcoma Cell Line by gene transfer
    PLOS ONE, 2012
    Co-Authors: Rounak Nande, Altomare Di Benedetto, Pierpaolo Aimola, Flavia De Carlo, Miranda B Carper
    Abstract:

    Fibrosarcoma is a deadly disease in cats and is significantly more often located at classical vaccine injections sites. More rare forms of spontaneous non-vaccination site (NSV) Fibrosarcomas have been described and have been found associated to genetic alterations. Purpose of this study was to compare the efficacy of adenoviral gene transfer in NVS Fibrosarcoma. We isolated and characterized a NVS Fibrosarcoma Cell Line (Cocca-6A) from a spontaneous Fibrosarcoma that occurred in a domestic calico cat. The feLine Cells were karyotyped and their chromosome number was counted using a Giemsa staining. Adenoviral gene transfer was verified by western blot analysis. Flow cytometry assay and Annexin-V were used to study Cell-cycle changes and Cell death of transduced Cells. Cocca-6A Fibrosarcoma Cells were morphologically and cytogenetically characterized. Giemsa block staining of metaphase spreads of the Cocca-6A Cells showed deletion of one of the E1 chromosomes, where feLine p53 maps. Semi-quantitative PCR demonstrated reduction of p53 genomic DNA in the Cocca-6A Cells. Adenoviral gene transfer determined a remarkable effect on the viability and growth of the Cocca-6A Cells following single transduction with adenoviruses carrying Mda-7/IL-24 or IFN-γ or various combination of RB/p105, Ras-DN, IFN-γ, and Mda-7 gene transfer. Therapy for feLine Fibrosarcomas is often insufficient for long lasting tumor eradication. More gene transfer studies should be conducted in order to understand if these viral vectors could be applicable regardless the origin (spontaneous vs. vaccine induced) of feLine Fibrosarcomas.

Pierpaolo Aimola - One of the best experts on this subject based on the ideXlab platform.

  • targeting a newly established spontaneous feLine Fibrosarcoma Cell Line by gene transfer
    PLOS ONE, 2012
    Co-Authors: Rounak Nande, Altomare Di Benedetto, Pierpaolo Aimola, Flavia De Carlo, Miranda B Carper
    Abstract:

    Fibrosarcoma is a deadly disease in cats and is significantly more often located at classical vaccine injections sites. More rare forms of spontaneous non-vaccination site (NSV) Fibrosarcomas have been described and have been found associated to genetic alterations. Purpose of this study was to compare the efficacy of adenoviral gene transfer in NVS Fibrosarcoma. We isolated and characterized a NVS Fibrosarcoma Cell Line (Cocca-6A) from a spontaneous Fibrosarcoma that occurred in a domestic calico cat. The feLine Cells were karyotyped and their chromosome number was counted using a Giemsa staining. Adenoviral gene transfer was verified by western blot analysis. Flow cytometry assay and Annexin-V were used to study Cell-cycle changes and Cell death of transduced Cells. Cocca-6A Fibrosarcoma Cells were morphologically and cytogenetically characterized. Giemsa block staining of metaphase spreads of the Cocca-6A Cells showed deletion of one of the E1 chromosomes, where feLine p53 maps. Semi-quantitative PCR demonstrated reduction of p53 genomic DNA in the Cocca-6A Cells. Adenoviral gene transfer determined a remarkable effect on the viability and growth of the Cocca-6A Cells following single transduction with adenoviruses carrying Mda-7/IL-24 or IFN-γ or various combination of RB/p105, Ras-DN, IFN-γ, and Mda-7 gene transfer. Therapy for feLine Fibrosarcomas is often insufficient for long lasting tumor eradication. More gene transfer studies should be conducted in order to understand if these viral vectors could be applicable regardless the origin (spontaneous vs. vaccine induced) of feLine Fibrosarcomas.

Miranda B Carper - One of the best experts on this subject based on the ideXlab platform.

  • targeting a newly established spontaneous feLine Fibrosarcoma Cell Line by gene transfer
    PLOS ONE, 2012
    Co-Authors: Rounak Nande, Altomare Di Benedetto, Pierpaolo Aimola, Flavia De Carlo, Miranda B Carper
    Abstract:

    Fibrosarcoma is a deadly disease in cats and is significantly more often located at classical vaccine injections sites. More rare forms of spontaneous non-vaccination site (NSV) Fibrosarcomas have been described and have been found associated to genetic alterations. Purpose of this study was to compare the efficacy of adenoviral gene transfer in NVS Fibrosarcoma. We isolated and characterized a NVS Fibrosarcoma Cell Line (Cocca-6A) from a spontaneous Fibrosarcoma that occurred in a domestic calico cat. The feLine Cells were karyotyped and their chromosome number was counted using a Giemsa staining. Adenoviral gene transfer was verified by western blot analysis. Flow cytometry assay and Annexin-V were used to study Cell-cycle changes and Cell death of transduced Cells. Cocca-6A Fibrosarcoma Cells were morphologically and cytogenetically characterized. Giemsa block staining of metaphase spreads of the Cocca-6A Cells showed deletion of one of the E1 chromosomes, where feLine p53 maps. Semi-quantitative PCR demonstrated reduction of p53 genomic DNA in the Cocca-6A Cells. Adenoviral gene transfer determined a remarkable effect on the viability and growth of the Cocca-6A Cells following single transduction with adenoviruses carrying Mda-7/IL-24 or IFN-γ or various combination of RB/p105, Ras-DN, IFN-γ, and Mda-7 gene transfer. Therapy for feLine Fibrosarcomas is often insufficient for long lasting tumor eradication. More gene transfer studies should be conducted in order to understand if these viral vectors could be applicable regardless the origin (spontaneous vs. vaccine induced) of feLine Fibrosarcomas.

Jacqueline I Alvarezleite - One of the best experts on this subject based on the ideXlab platform.

  • apoptosis induced by butyrate is independent of jak stat signaling in a Fibrosarcoma Cell Line
    Biochemical and Biophysical Research Communications, 2003
    Co-Authors: Flavia L A Rabelo, Mariana Gontijo Ramos, Gabriela Brumatti, Gustavo P Amarantemendes, Catherine Ropert, Claudio A Bonjardim, Jacqueline I Alvarezleite
    Abstract:

    The aim of this study was to evaluate the participation of the Jak-1 and STAT-1 proteins in sodium butyrate-induced apoptosis in 2C4 Cells derived from human Fibrosarcoma. Making use of Jak-1 or STAT-1 deficient Cell Lines, we demonstrated that the apoptotic process induced by butyrate is independent of the presence of these proteins. In addition, this work showed that, although the constitutive expression of pro-caspases-2 and -3 is reduced in STAT-1 Cells, the activity of caspase-3 is preserved in both Jak-1 and STAT-1 deficient Cells and is similar to that seen in 2C4 parental Cells. In conclusion, we demonstrated that the absence of functionally active Jak-1 or STAT-1 protein directly affects the TNF-alpha-induced apoptosis, but does not alter the sodium butyrate-induced apoptosis in Cells derived from human Fibrosarcoma.