The Experts below are selected from a list of 231 Experts worldwide ranked by ideXlab platform
Yiqi Yang - One of the best experts on this subject based on the ideXlab platform.
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Correction: Potent and regularizable crosslinking of ultrafine Fibrous Protein scaffolds for tissue engineering using a cytocompatible disaccharide derivative.
Journal of materials chemistry. B, 2020Co-Authors: Peng Liu, Yiqi YangAbstract:Correction for 'Potent and regularizable crosslinking of ultrafine Fibrous Protein scaffolds for tissue engineering using a cytocompatible disaccharide derivative' by Helan Xu et al., J. Mater. Chem. B, 2015, 3, 3609-3616, DOI: 10.1039/C4TB02100B.
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Erratum: Potent and regularizable crosslinking of ultrafine Fibrous Protein scaffolds for tissue engineering using a cytocompatible disaccharide derivative(Journal of Materials Chemistry B (2015) 3 (3609–3616) DOI: 10.1039/C4TB02100B)
Journal of materials chemistry. B, 2020Co-Authors: Peng Liu, Yiqi YangAbstract:Correction for 'Potent and regularizable crosslinking of ultrafine Fibrous Protein scaffolds for tissue engineering using a cytocompatible disaccharide derivative' by Helan Xu et al., J. Mater. Chem. B, 2015, 3, 3609-3616, DOI: 10.1039/C4TB02100B.
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potent and regularizable crosslinking of ultrafine Fibrous Protein scaffolds for tissue engineering using a cytocompatible disaccharide derivative
Journal of Materials Chemistry B, 2015Co-Authors: Peng Liu, Yiqi YangAbstract:Sucrose, a naturally-occurring disaccharide, could be oxidized to polar polyaldehydes to improve the performance properties of tissue engineering scaffolds composed of three-dimensionally arranged ultrafine Protein fibers in a controllable manner. With significantly better water stability, an in vitro study demonstrated that the biocompatibility of the oxidized sucrose crosslinked scaffolds was similar to the citric acid crosslinked ones. Due to their structural similarity to the major component in native extracellular matrices (ECMs), Proteins had advantages over other macromolecules for development of tissue engineering scaffolds. We have successfully developed three-dimensional (3D) ultrafine Fibrous structures from Proteins that could mimic the authentic 3D architectures of native ECMs. However, the enlarged contacting area exposed to water worsened the poor water stability of Proteins, and thus necessitated potent and non-toxic crosslinking. Citric acid, a biobased crosslinker, was widely recognized as safe and showed good crosslinking efficiency among non-toxic crosslinkers for Proteins, though was still less potent than aldehydes. In this research, sucrose was oxidized to non-volatile polyaldehydes with high polarity and low toxicity. Compared to those crosslinked with citric acid, the 3D ultrafine Fibrous zein scaffolds crosslinked with oxidized sucrose showed significantly better water stability and similar cytocompatibility via an in vitro study with preosteoblasts. In summary, oxidized sucrose could be a safe and potent crosslinker to improve water stability of macromolecule-based materials for medical and industrial applications.
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Oxidized sucrose: A potent and biocompatible crosslinker for three-dimensional Fibrous Protein scaffolds
Macromolecular Materials and Engineering, 2015Co-Authors: Peng Liu, Yiqi YangAbstract:Oxidized sucrose, an un-traditional biobased crosslinker, was proved as effective as glutaraldehyde in improving water stability of ultrafine Fibrous Proteins without causing cytotoxicity. Proteins from agricultural byproducts, such as zein, are abundantly available for production of industrial and medical products, but are restricted by their poor water stability and wet properties. An effective and safe crosslinking method became indispensable for Protein-based materials, especially biomaterials. Fibrous basic units with sub-micron scale were critical for biomaterials to resemble native extracellular matrices (ECMs) structurally. In this research, sucrose was oxidized into polar polyaldehydes to crosslink ultrafine Fibrous scaffolds from corn Protein. The control groups were crosslinked with glutaraldehyde, a known highly effective crosslinker with considerable toxicity. With similar improvement in water stability, the oxidized sucrose crosslinked scaffolds showed much better cytocompatibility than the glutaraldehyde crosslinked ones via in vitro study with preosteoblast cells.
Peng Liu - One of the best experts on this subject based on the ideXlab platform.
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Correction: Potent and regularizable crosslinking of ultrafine Fibrous Protein scaffolds for tissue engineering using a cytocompatible disaccharide derivative.
Journal of materials chemistry. B, 2020Co-Authors: Peng Liu, Yiqi YangAbstract:Correction for 'Potent and regularizable crosslinking of ultrafine Fibrous Protein scaffolds for tissue engineering using a cytocompatible disaccharide derivative' by Helan Xu et al., J. Mater. Chem. B, 2015, 3, 3609-3616, DOI: 10.1039/C4TB02100B.
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Erratum: Potent and regularizable crosslinking of ultrafine Fibrous Protein scaffolds for tissue engineering using a cytocompatible disaccharide derivative(Journal of Materials Chemistry B (2015) 3 (3609–3616) DOI: 10.1039/C4TB02100B)
Journal of materials chemistry. B, 2020Co-Authors: Peng Liu, Yiqi YangAbstract:Correction for 'Potent and regularizable crosslinking of ultrafine Fibrous Protein scaffolds for tissue engineering using a cytocompatible disaccharide derivative' by Helan Xu et al., J. Mater. Chem. B, 2015, 3, 3609-3616, DOI: 10.1039/C4TB02100B.
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potent and regularizable crosslinking of ultrafine Fibrous Protein scaffolds for tissue engineering using a cytocompatible disaccharide derivative
Journal of Materials Chemistry B, 2015Co-Authors: Peng Liu, Yiqi YangAbstract:Sucrose, a naturally-occurring disaccharide, could be oxidized to polar polyaldehydes to improve the performance properties of tissue engineering scaffolds composed of three-dimensionally arranged ultrafine Protein fibers in a controllable manner. With significantly better water stability, an in vitro study demonstrated that the biocompatibility of the oxidized sucrose crosslinked scaffolds was similar to the citric acid crosslinked ones. Due to their structural similarity to the major component in native extracellular matrices (ECMs), Proteins had advantages over other macromolecules for development of tissue engineering scaffolds. We have successfully developed three-dimensional (3D) ultrafine Fibrous structures from Proteins that could mimic the authentic 3D architectures of native ECMs. However, the enlarged contacting area exposed to water worsened the poor water stability of Proteins, and thus necessitated potent and non-toxic crosslinking. Citric acid, a biobased crosslinker, was widely recognized as safe and showed good crosslinking efficiency among non-toxic crosslinkers for Proteins, though was still less potent than aldehydes. In this research, sucrose was oxidized to non-volatile polyaldehydes with high polarity and low toxicity. Compared to those crosslinked with citric acid, the 3D ultrafine Fibrous zein scaffolds crosslinked with oxidized sucrose showed significantly better water stability and similar cytocompatibility via an in vitro study with preosteoblasts. In summary, oxidized sucrose could be a safe and potent crosslinker to improve water stability of macromolecule-based materials for medical and industrial applications.
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Oxidized sucrose: A potent and biocompatible crosslinker for three-dimensional Fibrous Protein scaffolds
Macromolecular Materials and Engineering, 2015Co-Authors: Peng Liu, Yiqi YangAbstract:Oxidized sucrose, an un-traditional biobased crosslinker, was proved as effective as glutaraldehyde in improving water stability of ultrafine Fibrous Proteins without causing cytotoxicity. Proteins from agricultural byproducts, such as zein, are abundantly available for production of industrial and medical products, but are restricted by their poor water stability and wet properties. An effective and safe crosslinking method became indispensable for Protein-based materials, especially biomaterials. Fibrous basic units with sub-micron scale were critical for biomaterials to resemble native extracellular matrices (ECMs) structurally. In this research, sucrose was oxidized into polar polyaldehydes to crosslink ultrafine Fibrous scaffolds from corn Protein. The control groups were crosslinked with glutaraldehyde, a known highly effective crosslinker with considerable toxicity. With similar improvement in water stability, the oxidized sucrose crosslinked scaffolds showed much better cytocompatibility than the glutaraldehyde crosslinked ones via in vitro study with preosteoblast cells.
David L Kaplan - One of the best experts on this subject based on the ideXlab platform.
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Fibrous Proteins: At the crossroads of genetic engineering and biotechnological applications.
Biotechnology and bioengineering, 2015Co-Authors: Sezin Yigit, Nina Dinjaski, David L KaplanAbstract:Fibrous Proteins, such as silk, elastin and collagen are finding broad impact in biomaterial systems for a range of biomedical and industrial applications. Some of the key advantages of biosynthetic Fibrous Proteins compared to synthetic polymers include the tailorability of sequence, Protein size, degradation pattern, and mechanical properties. Recombinant DNA production and precise control over genetic sequence of these Proteins allows expansion and fine tuning of material properties to meet the needs for specific applications. We review current approaches in the design, cloning, and expression of Fibrous Proteins, with a focus on strategies utilized to meet the challenges of repetitive Fibrous Protein production. We discuss recent advances in understanding the fundamental basis of structure-function relationships and the designs that foster Fibrous Protein self-assembly towards predictable architectures and properties for a range of applications. We highlight the potential of functionalization through genetic engineering to design Fibrous Protein systems for biotechnological and biomedical applications.
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Microphase Separation Controlled β-Sheet Crystallization Kinetics in Fibrous Proteins
Macromolecules, 2009Co-Authors: David L Kaplan, Peggy CebeAbstract:Silk is a naturally occurring Fibrous Protein with a multiblock chain architecture. As such, it has many similarities with synthetic block copolymers, including the possibility for β-sheet crystall...
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FiberID--a technique to identify Fibrous Protein subclasses.
Proteins, 2007Co-Authors: Peter Waltman, Anselm Blumer, David L KaplanAbstract:Fibrous Proteins such as collagen, silk, and elastin play critical biological roles, yet they have been the subject of few projects that use computational techniques to predict either their class or their structure. In this article, we present FiberID, a simple yet effective method for identifying and distinguishing three Fibrous Protein subclasses from their primary sequences. Using a combination of amino acid composition and fast Fourier measurements, FiberID can classify Fibrous Proteins belonging to these subclasses with high accuracy by using two standard machine learning techniques (decision trees and Naive Bayesian classifiers). After presenting our results, we present several Fibrous sequences that are regularly misclassified by FiberID as sequences of potential interest for further study. Finally, we analyze the decision trees developed by FiberID for potential insights regarding the structure of these Proteins. Proteins 2007. © 2006 Wiley-Liss, Inc.
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Surface organization and nanopatterning of collagen by dip-pen nanolithography.
Proceedings of the National Academy of Sciences of the United States of America, 2001Co-Authors: Donna L. Wilson, Seunghun Hong, Raquel Martin, Chad A. Mirkin, Mark Cronin-golomb, David L KaplanAbstract:Collagen is a key Fibrous Protein in biological systems, characterized by a complex structural hierarchy as well as the ability to self-assemble into liquid crystalline mesophases. The structural features of collagen influence cellular responses and material properties, with importance for a wide range of biomaterials and tissue architectures. The mechanism by which fibrillar collagen structures form from liquid crystalline mesophases is not well characterized. We report positive printing of collagen and a collagen-like peptide down to 30–50-nm line widths, using the atomic force microscopy technique of dip-pen nanolithography. The method preserved the triple-helical structure and biological activity of collagen and even fostered the formation of characteristic higher levels of structural organization. The “direct-write” capability of biologically relevant molecules, while preserving their structure and functionality, provides tremendous flexibility in future biological device applications and in proteomics arrays, as well as a new strategy to study the important hierarchical assembly processes of biological systems.
Aldo R Boccaccini - One of the best experts on this subject based on the ideXlab platform.
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Fibrous Protein-based biomaterials (silk, keratin, elastin, and resilin Proteins) for tissue regeneration and repair
Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair, 2018Co-Authors: F. Costa, Raquel Silva, Aldo R BoccacciniAbstract:Abstract Tissue scaffolds are essential in tissue engineering approaches as they provide a tissue-mimicking environment for cells, with the aim to promote cell proliferation, cell differentiation, and tissue regeneration. To mimic better the microenvironment of native tissues, numerous techniques, and biomaterials have emerged in recent years. Among them, hydrogels formed from self-assembled biopolymer networks are particularly interesting. This chapter reviews the fabrication and use of Fibrous Protein-based hydrogels, with an emphasis on silk, keratin, elastin, and resilin Proteins. Hydrogels formed by these Proteins show structural, chemical, and mechanical similarities with the extracellular matrix of human tissues, typically exhibiting biological compatibility, and they can trigger specific cellular responses. In addition, these hydrogels can be degraded in the body by proteolytic enzymes. For these reasons, Fibrous Protein hydrogels are versatile materials for tissue engineering.
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Fibrous Protein based hydrogels for cell encapsulation
Biomaterials, 2014Co-Authors: Raquel Silva, Ben Fabry, Aldo R BoccacciniAbstract:Tissue scaffolds play a vital role in tissue engineering by providing a native tissue-mimicking environment for cells, with the aim to promote cell proliferation, proper cell differentiation, and regeneration. To better mimic the microenvironment of native tissues, novel techniques and materials have emerged in recent years. Among them, hydrogels formed from self-assembled biopolymer networks are particularly interesting. This paper reviews the fabrication and use of Fibrous Protein-based hydrogels, with an emphasis on silk, keratin elastin and resilin Proteins. Hydrogels formed by these Proteins show close structural, chemical and mechanical similarities with the extracellular matrix, typically good biological compatibility, and they can trigger specific cellular responses. In addition, these hydrogels can be degraded in the body by proteolytic enzymes. For these reasons, Fibrous Protein hydrogels are one of the most versatile materials for tissue engineering.
Raquel Silva - One of the best experts on this subject based on the ideXlab platform.
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Fibrous Protein-based biomaterials (silk, keratin, elastin, and resilin Proteins) for tissue regeneration and repair
Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair, 2018Co-Authors: F. Costa, Raquel Silva, Aldo R BoccacciniAbstract:Abstract Tissue scaffolds are essential in tissue engineering approaches as they provide a tissue-mimicking environment for cells, with the aim to promote cell proliferation, cell differentiation, and tissue regeneration. To mimic better the microenvironment of native tissues, numerous techniques, and biomaterials have emerged in recent years. Among them, hydrogels formed from self-assembled biopolymer networks are particularly interesting. This chapter reviews the fabrication and use of Fibrous Protein-based hydrogels, with an emphasis on silk, keratin, elastin, and resilin Proteins. Hydrogels formed by these Proteins show structural, chemical, and mechanical similarities with the extracellular matrix of human tissues, typically exhibiting biological compatibility, and they can trigger specific cellular responses. In addition, these hydrogels can be degraded in the body by proteolytic enzymes. For these reasons, Fibrous Protein hydrogels are versatile materials for tissue engineering.
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Fibrous Protein based hydrogels for cell encapsulation
Biomaterials, 2014Co-Authors: Raquel Silva, Ben Fabry, Aldo R BoccacciniAbstract:Tissue scaffolds play a vital role in tissue engineering by providing a native tissue-mimicking environment for cells, with the aim to promote cell proliferation, proper cell differentiation, and regeneration. To better mimic the microenvironment of native tissues, novel techniques and materials have emerged in recent years. Among them, hydrogels formed from self-assembled biopolymer networks are particularly interesting. This paper reviews the fabrication and use of Fibrous Protein-based hydrogels, with an emphasis on silk, keratin elastin and resilin Proteins. Hydrogels formed by these Proteins show close structural, chemical and mechanical similarities with the extracellular matrix, typically good biological compatibility, and they can trigger specific cellular responses. In addition, these hydrogels can be degraded in the body by proteolytic enzymes. For these reasons, Fibrous Protein hydrogels are one of the most versatile materials for tissue engineering.