The Experts below are selected from a list of 177 Experts worldwide ranked by ideXlab platform
Tomoji Kawai - One of the best experts on this subject based on the ideXlab platform.
-
high resolution scanning tunneling microscopy and di dv map studies of peptidenucleic acid and Fluorescein Isothiocyanate
Applied Surface Science, 2006Co-Authors: Hiroyuki Tanaka, Tomoji KawaiAbstract:Abstract We used scanning tunneling microscopy (STM) to spatially map the local density of states of individual PNA molecules labeled with Fluorescein Isothiocyanate (FITC) on a Cu(1 1 1) surface. From the observed bias voltage dependences of the topographic height and the dI/dV map of FITC and individual PNA molecules, we confirmed that FITC and PNA have different electrical properties. We clearly differentiated the FITC and PNA molecules by mapping the density of states feature. This study shows that STM with the dI/dV map method is useful in FITC mapping.
-
high resolution scanning tunneling microscopy and spectroscopy studies of deoxyribonucleic acid and Fluorescein Isothiocyanate
Japanese Journal of Applied Physics, 2004Co-Authors: Yoshihiro Nojima, Hiroyuki Tanaka, Y Yoshida, Tomoji KawaiAbstract:By using scanning tunneling microscopy (STM), we obtained submolecular-resolution STM images of a single molecule of DNA labeled with the fluorescent dye Fluorescein Isothiocyanate (FITC). From the observed bias voltage dependences of the topographic height and I–V characteristics of FITC and the single molecule DNA, we found that FITC and DNA have different electrical properties. This study shows that STM is useful in the analysis of the properties of molecules by the investigation of a single molecule.
Xi Chen - One of the best experts on this subject based on the ideXlab platform.
-
encoding electrochemiluminescence using ru bpy 32 and Fluorescein Isothiocyanate co doped silica nanoparticles
Chemical Communications, 2011Co-Authors: Lan Luan, Genghuang Wu, Xiaoli Huang, Xi ChenAbstract:In this paper, an electrochemiluminescence (ECL) encoding method was developed for the first time, based on a dual-dye system including Ru(bpy)32+ as an ECL emitter and Fluorescein Isothiocyanate (FITC) as a coding dye.
-
Encoding electrochemiluminescence using Ru(bpy)32+ and Fluorescein Isothiocyanate co-doped silica nanoparticles
Chemical Communications, 2011Co-Authors: Lan Luan, Genghuang Wu, Xiaoli Huang, Xi ChenAbstract:In this paper, an electrochemiluminescence (ECL) encoding method was developed for the first time, based on a dual-dye system including Ru(bpy)32+ as an ECL emitter and Fluorescein Isothiocyanate (FITC) as a coding dye.
Hiroyuki Tanaka - One of the best experts on this subject based on the ideXlab platform.
-
high resolution scanning tunneling microscopy and di dv map studies of peptidenucleic acid and Fluorescein Isothiocyanate
Applied Surface Science, 2006Co-Authors: Hiroyuki Tanaka, Tomoji KawaiAbstract:Abstract We used scanning tunneling microscopy (STM) to spatially map the local density of states of individual PNA molecules labeled with Fluorescein Isothiocyanate (FITC) on a Cu(1 1 1) surface. From the observed bias voltage dependences of the topographic height and the dI/dV map of FITC and individual PNA molecules, we confirmed that FITC and PNA have different electrical properties. We clearly differentiated the FITC and PNA molecules by mapping the density of states feature. This study shows that STM with the dI/dV map method is useful in FITC mapping.
-
high resolution scanning tunneling microscopy and spectroscopy studies of deoxyribonucleic acid and Fluorescein Isothiocyanate
Japanese Journal of Applied Physics, 2004Co-Authors: Yoshihiro Nojima, Hiroyuki Tanaka, Y Yoshida, Tomoji KawaiAbstract:By using scanning tunneling microscopy (STM), we obtained submolecular-resolution STM images of a single molecule of DNA labeled with the fluorescent dye Fluorescein Isothiocyanate (FITC). From the observed bias voltage dependences of the topographic height and I–V characteristics of FITC and the single molecule DNA, we found that FITC and DNA have different electrical properties. This study shows that STM is useful in the analysis of the properties of molecules by the investigation of a single molecule.
Lee Josephson - One of the best experts on this subject based on the ideXlab platform.
-
Fluorescein Isothiocyanate–hapten immunoassay for determination of peptide–cell interactions
Analytical Biochemistry, 2004Co-Authors: Kimberly A. Kelly, Fred Reynolds, Ralph Weissleder, Lee JosephsonAbstract:Abstract We have developed a Fluorescein Isothiocyanate (FITC)–hapten immunoassay, where a FITC-labeled peptide binding to a cell is assayed as the amount of immunoreactive Fluorescein present in a cell lysate. An antiFluorescein–horseradish peroxidase conjugate binds to either a Fluoresceinated peptide in the lysate or a Fluorescein attached to the wells of a microtiter plate in a competitive fashion. After washing, solid-phase peroxidase activity is measured and inversely related to the amount of FITC-labeled peptide present. To demonstrate the assay, the interaction of a FITC-labeled bombesin-like peptide with the gastrin-releasing peptide receptor on PC-3 and HT-29 cells was investigated. Using PC-3 cells, we obtained similar displacement curves and numbers of binding sites per cell by both the FITC–hapten immunoassay and a reference radioreceptor assay. The FITC–hapten immunoassay is a sensitive and versatile method, since the same commercially available reagents can be used to assess interactions between any peptide and any receptor. In addition, the FITC-labeled peptide can be used to visualize receptors in fluorescent-activated cell sorting or fluorescent microscopy.
-
Fluorescein Isothiocyanate hapten immunoassay for determination of peptide cell interactions
Analytical Biochemistry, 2004Co-Authors: Kimberly A. Kelly, Fred Reynolds, Ralph Weissleder, Lee JosephsonAbstract:Abstract We have developed a Fluorescein Isothiocyanate (FITC)–hapten immunoassay, where a FITC-labeled peptide binding to a cell is assayed as the amount of immunoreactive Fluorescein present in a cell lysate. An antiFluorescein–horseradish peroxidase conjugate binds to either a Fluoresceinated peptide in the lysate or a Fluorescein attached to the wells of a microtiter plate in a competitive fashion. After washing, solid-phase peroxidase activity is measured and inversely related to the amount of FITC-labeled peptide present. To demonstrate the assay, the interaction of a FITC-labeled bombesin-like peptide with the gastrin-releasing peptide receptor on PC-3 and HT-29 cells was investigated. Using PC-3 cells, we obtained similar displacement curves and numbers of binding sites per cell by both the FITC–hapten immunoassay and a reference radioreceptor assay. The FITC–hapten immunoassay is a sensitive and versatile method, since the same commercially available reagents can be used to assess interactions between any peptide and any receptor. In addition, the FITC-labeled peptide can be used to visualize receptors in fluorescent-activated cell sorting or fluorescent microscopy.
Lan Luan - One of the best experts on this subject based on the ideXlab platform.
-
encoding electrochemiluminescence using ru bpy 32 and Fluorescein Isothiocyanate co doped silica nanoparticles
Chemical Communications, 2011Co-Authors: Lan Luan, Genghuang Wu, Xiaoli Huang, Xi ChenAbstract:In this paper, an electrochemiluminescence (ECL) encoding method was developed for the first time, based on a dual-dye system including Ru(bpy)32+ as an ECL emitter and Fluorescein Isothiocyanate (FITC) as a coding dye.
-
Encoding electrochemiluminescence using Ru(bpy)32+ and Fluorescein Isothiocyanate co-doped silica nanoparticles
Chemical Communications, 2011Co-Authors: Lan Luan, Genghuang Wu, Xiaoli Huang, Xi ChenAbstract:In this paper, an electrochemiluminescence (ECL) encoding method was developed for the first time, based on a dual-dye system including Ru(bpy)32+ as an ECL emitter and Fluorescein Isothiocyanate (FITC) as a coding dye.