The Experts below are selected from a list of 1161 Experts worldwide ranked by ideXlab platform
P. Biver - One of the best experts on this subject based on the ideXlab platform.
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human placenta Radioreceptor Assay with digoxin and ouabain to detect endogenous digitalis like factor s in human plasma and urine
Clinical Chemistry, 1996Co-Authors: Alexandre Paci, Giuliano Ciarimboli, P. BiverAbstract:We describe the optimization and validation of a clinically feasible Radioreceptor Assay to detect endogenous digitalis-like factor(s) (EDLF) in human plasma and urine. The Assay is based on the competitive replacement of 125I-labeled digoxin on human placenta membranes by ligands present in sample extracts. Digoxin and ouabain were used as calibrators. We also describe simple and effective methods for extraction and enrichment of EDLF from human plasma and urine. Assay sensitivity and precision were enhanced by using a sequential saturation technique with appropriate concentrations of tracer and receptors. Filtration was used to separate bound from free ligand. A two-step solid-state extraction with acetonitrile allowed the separation of two EDLFs with different polarity (EDLF-1 and EDLF-2) from the same plasma sample. A one-step solid-state extraction with methanol was suitable for urine. EDLF-1 and EDLF-2 in healthy adults were respectively 204 +/- 155 and 207 +/- 423 pmol/L ouabain equivalents, or 312 +/- 241 and 302 +/- 581 pmol/L digoxin equivalents. Plasma concentrations of EDLFs in newborns and pregnant women were higher than in healthy adults, and the concentrations in urine were higher than in plasma. Several cross-reactivity experiments showed that physiological concentrations of endogenous steroids and lipids did not inhibit binding, and supported the hypothesis that EDLFs are endogenous compounds other than the steroids and lipids also investigated.
Lewis E Braverman - One of the best experts on this subject based on the ideXlab platform.
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serum thyrotropin receptor antibodies concentrations in patients with graves disease before at the end of methimazole treatment and after drug withdrawal evidence that the activity of thyrotropin receptor antibody and or thyroid response modify durin
Thyroid, 2006Co-Authors: Carlo Carella, Gherardo Mazziotti, F Sorvillo, Marco Piscopo, Michele Cioffi, Paola Pilla, Roberto Nersita, Sergio Iorio, G Amato, Lewis E BravermanAbstract:Aim and methods: We performed a quantitative retrospective analysis of serum thyrotropin receptor antibody (TRAb) concentrations measured by a second-generation Radioreceptor Assay in 58 patients w...
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serum thyrotropin receptor antibodies concentrations in patients with graves disease before at the end of methimazole treatment and after drug withdrawal evidence that the activity of thyrotropin receptor antibody and or thyroid response modify durin
Thyroid, 2006Co-Authors: Carlo Carella, Gherardo Mazziotti, F Sorvillo, Marco Piscopo, Michele Cioffi, Paola Pilla, Roberto Nersita, Sergio Iorio, G Amato, Lewis E BravermanAbstract:Aim and methods: We performed a quantitative retrospective analysis of serum thyrotropin receptor antibody (TRAb) concentrations measured by a second-generation Radioreceptor Assay in 58 patients with Graves' disease (GD) at the onset of the disease, at the end of 18 month methimazole (MMI) treatment, and after MMI withdrawal in order to evaluate the correlation between the presence of these antibodies and the relapse of hyperthyroidism. Sixty healthy subjects were enrolled as a control group. Results: Before MMI treatment the best cutoff TRAb value for identifying patients with GD was 1.45 UI/L (specificity, 100%; sensitivity, 98.3%). At the end of MMI treatment, serum TRAb concentrations were significantly lower (p 1.45 UI/L). After MMI withdrawal (median, 15 months), 34 patients (58.6%) became...
Alexandre Paci - One of the best experts on this subject based on the ideXlab platform.
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human placenta Radioreceptor Assay with digoxin and ouabain to detect endogenous digitalis like factor s in human plasma and urine
Clinical Chemistry, 1996Co-Authors: Alexandre Paci, Giuliano Ciarimboli, P. BiverAbstract:We describe the optimization and validation of a clinically feasible Radioreceptor Assay to detect endogenous digitalis-like factor(s) (EDLF) in human plasma and urine. The Assay is based on the competitive replacement of 125I-labeled digoxin on human placenta membranes by ligands present in sample extracts. Digoxin and ouabain were used as calibrators. We also describe simple and effective methods for extraction and enrichment of EDLF from human plasma and urine. Assay sensitivity and precision were enhanced by using a sequential saturation technique with appropriate concentrations of tracer and receptors. Filtration was used to separate bound from free ligand. A two-step solid-state extraction with acetonitrile allowed the separation of two EDLFs with different polarity (EDLF-1 and EDLF-2) from the same plasma sample. A one-step solid-state extraction with methanol was suitable for urine. EDLF-1 and EDLF-2 in healthy adults were respectively 204 +/- 155 and 207 +/- 423 pmol/L ouabain equivalents, or 312 +/- 241 and 302 +/- 581 pmol/L digoxin equivalents. Plasma concentrations of EDLFs in newborns and pregnant women were higher than in healthy adults, and the concentrations in urine were higher than in plasma. Several cross-reactivity experiments showed that physiological concentrations of endogenous steroids and lipids did not inhibit binding, and supported the hypothesis that EDLFs are endogenous compounds other than the steroids and lipids also investigated.
Carlo Carella - One of the best experts on this subject based on the ideXlab platform.
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serum thyrotropin receptor antibodies concentrations in patients with graves disease before at the end of methimazole treatment and after drug withdrawal evidence that the activity of thyrotropin receptor antibody and or thyroid response modify durin
Thyroid, 2006Co-Authors: Carlo Carella, Gherardo Mazziotti, F Sorvillo, Marco Piscopo, Michele Cioffi, Paola Pilla, Roberto Nersita, Sergio Iorio, G Amato, Lewis E BravermanAbstract:Aim and methods: We performed a quantitative retrospective analysis of serum thyrotropin receptor antibody (TRAb) concentrations measured by a second-generation Radioreceptor Assay in 58 patients w...
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serum thyrotropin receptor antibodies concentrations in patients with graves disease before at the end of methimazole treatment and after drug withdrawal evidence that the activity of thyrotropin receptor antibody and or thyroid response modify durin
Thyroid, 2006Co-Authors: Carlo Carella, Gherardo Mazziotti, F Sorvillo, Marco Piscopo, Michele Cioffi, Paola Pilla, Roberto Nersita, Sergio Iorio, G Amato, Lewis E BravermanAbstract:Aim and methods: We performed a quantitative retrospective analysis of serum thyrotropin receptor antibody (TRAb) concentrations measured by a second-generation Radioreceptor Assay in 58 patients with Graves' disease (GD) at the onset of the disease, at the end of 18 month methimazole (MMI) treatment, and after MMI withdrawal in order to evaluate the correlation between the presence of these antibodies and the relapse of hyperthyroidism. Sixty healthy subjects were enrolled as a control group. Results: Before MMI treatment the best cutoff TRAb value for identifying patients with GD was 1.45 UI/L (specificity, 100%; sensitivity, 98.3%). At the end of MMI treatment, serum TRAb concentrations were significantly lower (p 1.45 UI/L). After MMI withdrawal (median, 15 months), 34 patients (58.6%) became...
Giuliano Ciarimboli - One of the best experts on this subject based on the ideXlab platform.
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human placenta Radioreceptor Assay with digoxin and ouabain to detect endogenous digitalis like factor s in human plasma and urine
Clinical Chemistry, 1996Co-Authors: Alexandre Paci, Giuliano Ciarimboli, P. BiverAbstract:We describe the optimization and validation of a clinically feasible Radioreceptor Assay to detect endogenous digitalis-like factor(s) (EDLF) in human plasma and urine. The Assay is based on the competitive replacement of 125I-labeled digoxin on human placenta membranes by ligands present in sample extracts. Digoxin and ouabain were used as calibrators. We also describe simple and effective methods for extraction and enrichment of EDLF from human plasma and urine. Assay sensitivity and precision were enhanced by using a sequential saturation technique with appropriate concentrations of tracer and receptors. Filtration was used to separate bound from free ligand. A two-step solid-state extraction with acetonitrile allowed the separation of two EDLFs with different polarity (EDLF-1 and EDLF-2) from the same plasma sample. A one-step solid-state extraction with methanol was suitable for urine. EDLF-1 and EDLF-2 in healthy adults were respectively 204 +/- 155 and 207 +/- 423 pmol/L ouabain equivalents, or 312 +/- 241 and 302 +/- 581 pmol/L digoxin equivalents. Plasma concentrations of EDLFs in newborns and pregnant women were higher than in healthy adults, and the concentrations in urine were higher than in plasma. Several cross-reactivity experiments showed that physiological concentrations of endogenous steroids and lipids did not inhibit binding, and supported the hypothesis that EDLFs are endogenous compounds other than the steroids and lipids also investigated.
