The Experts below are selected from a list of 51024 Experts worldwide ranked by ideXlab platform
Jiaxing Huang - One of the best experts on this subject based on the ideXlab platform.
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visualizing graphene based sheets by Fluorescence Quenching microscopy
Journal of the American Chemical Society, 2010Co-Authors: Laura J. Cote, Jiaxing HuangAbstract:Graphene based sheets have stimulated great interest due to their superior mechanical, electrical, and thermal properties. A general visualization method that allows quick observation of these single atomic layers would be highly desirable as it can greatly facilitate sample evaluation and manipulation, and provide immediate feedback to improve synthesis and processing strategies. Here we report that graphene based sheets can be made highly visible under a Fluorescence microscope by Quenching the emission from a dye coating, which can be conveniently removed afterward by rinsing without disrupting the sheets. Current imaging techniques for graphene based sheets rely on the use of special substrates. In contrast, the Fluorescence Quenching mechanism is no longer limited by the type of substrate. Graphene, reduced graphene oxide, or even graphene oxide sheets deposited on arbitrary substrates can now be readily visualized with good contrast for layer counting. Direct observation of suspended sheets in solut...
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Visualizing graphene based sheets by Fluorescence Quenching microscopy
Journal of the American Chemical Society, 2010Co-Authors: Jaemyung Kim, Laura J. Cote, Franklin Kim, Jiaxing HuangAbstract:Graphene based sheets have stimulated great interest due to their superior mechanical, electrical, and thermal properties. A general visualization method that allows quick observation of these single atomic layers would be highly desirable as it can greatly facilitate sample evaluation and manipulation, and provide immediate feedback to improve synthesis and processing strategies. Here we report that graphene based sheets can be made highly visible under a Fluorescence microscope by Quenching the emission from a dye coating, which can be conveniently removed afterward by rinsing without disrupting the sheets. Current imaging techniques for graphene based sheets rely on the use of special substrates. In contrast, the Fluorescence Quenching mechanism is no longer limited by the type of substrate. Graphene, reduced graphene oxide, or even graphene oxide sheets deposited on arbitrary substrates can now be readily visualized with good contrast for layer counting. Direct observation of suspended sheets in solution was also demonstrated. The Fluorescence Quenching microscopy offers unprecedented imaging flexibility and could become a general tool for characterizing graphene based materials.
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visualizing graphene based sheets by Fluorescence Quenching microscopy
arXiv: Materials Science, 2009Co-Authors: Jaemyung Kim, Laura J. Cote, Franklin Kim, Jiaxing HuangAbstract:Graphene based sheets have stimulated great interest due to their superior mechanical, electrical and thermal properties. A general visualization method that allows quick observation of these single atomic layers would be highly desirable as it can greatly facilitate sample evaluation and manipulation, and provide immediate feedback to improve synthesis and processing strategies. Here we report that graphene based sheets can be made highly visible under a Fluorescence microscope by Quenching the emission from a dye coating, which can be conveniently removed afterwards by rinsing without disrupting the sheets. Current imaging techniques for graphene based sheets rely on the use of special substrates. In contrast, the Fluorescence Quenching mechanism is no longer limited by the types of substrates. Graphene, reduced graphene oxide, or even graphene oxide sheets deposited on arbitrary substrates can now be readily visualized by eye with good contrast for layer counting. Direct observation of suspended sheets in solution was also demonstrated. The Fluorescence Quenching microscopy offers unprecedented imaging flexibility and could become a general tool for characterizing graphene based materials.
Yonghua Xiong - One of the best experts on this subject based on the ideXlab platform.
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inner filter effect based Fluorescence Quenching immunochromotographic assay for sensitive detection of aflatoxin b1 in soybean sauce
Food Control, 2018Co-Authors: Hu Jiang, Wenjing Zhang, Lijuan Nie, Hong Duan, Yonghua XiongAbstract:Abstract A Fluorescence-Quenching immunochromotographic assay (ICA) was developed for sensitive detection of aflatoxin B1 (AFB1) in soybean sauce based on the inner filter effect (IFE) between flower-like gold nanoparticles (AuNFs) and quantum dots (QDs). QDs were sprayed on the test and control line zones as background Fluorescence signals, whereas AuNFs were designed as the Fluorescence absorber of QDs because the surface plasma resonance peak of AuNFs totally matched with the maximum emission peak of QDs. Under the optimal conditions, the Fluorescence-Quenching ICA strip showed a good linear detection for AFB1 in standard AFB1 solution from 0.008 μg/L to 1 μg/L with a low detection limit of 0.004 μg/L. The average recoveries for different concentrations of AFB1-spiked soybean sauce samples ranged from 84.69% to 120.44% with a coefficient of variation ranging from 2.73% to 10.41%. In addition, the reliability of the proposed method was further confirmed by ultra-performance liquid chromatography with Fluorescence detection method. In brief, this novel IFE-based strip offers a simple, rapid, sensitive, and accurate strategy for quantitative detection of AFB1 in soybean sauce.
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silver nanoparticle based Fluorescence Quenching lateral flow immunoassay for sensitive detection of ochratoxin a in grape juice and wine
Toxins, 2017Co-Authors: Hu Jiang, Lijuan Nie, Ying Xiong, Ke Pei, Yonghua XiongAbstract:A silver nanoparticle (AgNP)-based Fluorescence-Quenching lateral flow immunoassay with competitive format (cLFIA) was developed for sensitive detection of ochratoxin A (OTA) in grape juice and wine samples in the present study. The Ru(phen) 3 2 + -doped silica nanoparticles (RuNPs) were sprayed on the test and control line zones as background Fluorescence signals. The AgNPs were designed as the Fluorescence quenchers of RuNPs because they can block the exciting light transferring to the RuNP molecules. The proposed method exhibited high sensitivity for OTA detection, with a detection limit of 0.06 µg/L under optimized conditions. The method also exhibited a good linear range for OTA quantitative analysis from 0.08 µg/L to 5.0 µg/L. The reliability of the Fluorescence-Quenching cLFIA method was evaluated through analysis of the OTA-spiked red grape wine and juice samples. The average recoveries ranged from 88.0% to 110.0% in red grape wine and from 92.0% to 110.0% in grape juice. Meanwhile, less than a 10% coefficient variation indicated an acceptable precision of the cLFIA method. In summary, the new AgNP-based Fluorescence-Quenching cLFIA is a simple, rapid, sensitive, and accurate method for quantitative detection of OTA in grape juice and wine or other foodstuffs.
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ultrasensitive Fluorescence immunoassay for detection of ochratoxin a using catalase mediated Fluorescence Quenching of cdte qds
Nanoscale, 2016Co-Authors: Xiaolin Huang, Yonghua Xiong, Shengnan Zhan, Hengyi Xu, Xianwei Meng, Xiaoyuan ChenAbstract:Herein, for the first time we report an improved competitive fluorescent enzyme linked immunosorbent assay (ELISA) for the ultrasensitive detection of ochratoxin A (OTA) by using hydrogen peroxide (H2O2)-induced Fluorescence Quenching of mercaptopropionic acid-modified CdTe quantum dots (QDs). In this immunoassay, catalase (CAT) was labeled with OTA as a competitive antigen to connect the Fluorescence signals of the QDs with the concentration of the target. Through the combinatorial use of H2O2-induced Fluorescence Quenching of CdTe QDs as a Fluorescence signal output and the ultrahigh catalytic activity of CAT to H2O2, our proposed method could be used to perform a dynamic linear detection of OTA ranging from 0.05 pg mL−1 to 10 pg mL−1. The half maximal inhibitory concentration was 0.53 pg mL−1 and the limit of detection was 0.05 pg mL−1. These values were approximately 283- and 300-folds lower than those of horseradish peroxidase (HRP)-based conventional ELISA, respectively. The reported method is accurate, highly reproducible, and specific against other mycotoxins in agricultural products as well. In summary, the developed Fluorescence immunoassay based on H2O2-induced Fluorescence Quenching of CdTe QDs can be used for the rapid and highly sensitive detection of mycotoxins or haptens in food safety monitoring.
Laura J. Cote - One of the best experts on this subject based on the ideXlab platform.
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visualizing graphene based sheets by Fluorescence Quenching microscopy
Journal of the American Chemical Society, 2010Co-Authors: Laura J. Cote, Jiaxing HuangAbstract:Graphene based sheets have stimulated great interest due to their superior mechanical, electrical, and thermal properties. A general visualization method that allows quick observation of these single atomic layers would be highly desirable as it can greatly facilitate sample evaluation and manipulation, and provide immediate feedback to improve synthesis and processing strategies. Here we report that graphene based sheets can be made highly visible under a Fluorescence microscope by Quenching the emission from a dye coating, which can be conveniently removed afterward by rinsing without disrupting the sheets. Current imaging techniques for graphene based sheets rely on the use of special substrates. In contrast, the Fluorescence Quenching mechanism is no longer limited by the type of substrate. Graphene, reduced graphene oxide, or even graphene oxide sheets deposited on arbitrary substrates can now be readily visualized with good contrast for layer counting. Direct observation of suspended sheets in solut...
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Visualizing graphene based sheets by Fluorescence Quenching microscopy
Journal of the American Chemical Society, 2010Co-Authors: Jaemyung Kim, Laura J. Cote, Franklin Kim, Jiaxing HuangAbstract:Graphene based sheets have stimulated great interest due to their superior mechanical, electrical, and thermal properties. A general visualization method that allows quick observation of these single atomic layers would be highly desirable as it can greatly facilitate sample evaluation and manipulation, and provide immediate feedback to improve synthesis and processing strategies. Here we report that graphene based sheets can be made highly visible under a Fluorescence microscope by Quenching the emission from a dye coating, which can be conveniently removed afterward by rinsing without disrupting the sheets. Current imaging techniques for graphene based sheets rely on the use of special substrates. In contrast, the Fluorescence Quenching mechanism is no longer limited by the type of substrate. Graphene, reduced graphene oxide, or even graphene oxide sheets deposited on arbitrary substrates can now be readily visualized with good contrast for layer counting. Direct observation of suspended sheets in solution was also demonstrated. The Fluorescence Quenching microscopy offers unprecedented imaging flexibility and could become a general tool for characterizing graphene based materials.
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visualizing graphene based sheets by Fluorescence Quenching microscopy
arXiv: Materials Science, 2009Co-Authors: Jaemyung Kim, Laura J. Cote, Franklin Kim, Jiaxing HuangAbstract:Graphene based sheets have stimulated great interest due to their superior mechanical, electrical and thermal properties. A general visualization method that allows quick observation of these single atomic layers would be highly desirable as it can greatly facilitate sample evaluation and manipulation, and provide immediate feedback to improve synthesis and processing strategies. Here we report that graphene based sheets can be made highly visible under a Fluorescence microscope by Quenching the emission from a dye coating, which can be conveniently removed afterwards by rinsing without disrupting the sheets. Current imaging techniques for graphene based sheets rely on the use of special substrates. In contrast, the Fluorescence Quenching mechanism is no longer limited by the types of substrates. Graphene, reduced graphene oxide, or even graphene oxide sheets deposited on arbitrary substrates can now be readily visualized by eye with good contrast for layer counting. Direct observation of suspended sheets in solution was also demonstrated. The Fluorescence Quenching microscopy offers unprecedented imaging flexibility and could become a general tool for characterizing graphene based materials.
Hu Jiang - One of the best experts on this subject based on the ideXlab platform.
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inner filter effect based Fluorescence Quenching immunochromotographic assay for sensitive detection of aflatoxin b1 in soybean sauce
Food Control, 2018Co-Authors: Hu Jiang, Wenjing Zhang, Lijuan Nie, Hong Duan, Yonghua XiongAbstract:Abstract A Fluorescence-Quenching immunochromotographic assay (ICA) was developed for sensitive detection of aflatoxin B1 (AFB1) in soybean sauce based on the inner filter effect (IFE) between flower-like gold nanoparticles (AuNFs) and quantum dots (QDs). QDs were sprayed on the test and control line zones as background Fluorescence signals, whereas AuNFs were designed as the Fluorescence absorber of QDs because the surface plasma resonance peak of AuNFs totally matched with the maximum emission peak of QDs. Under the optimal conditions, the Fluorescence-Quenching ICA strip showed a good linear detection for AFB1 in standard AFB1 solution from 0.008 μg/L to 1 μg/L with a low detection limit of 0.004 μg/L. The average recoveries for different concentrations of AFB1-spiked soybean sauce samples ranged from 84.69% to 120.44% with a coefficient of variation ranging from 2.73% to 10.41%. In addition, the reliability of the proposed method was further confirmed by ultra-performance liquid chromatography with Fluorescence detection method. In brief, this novel IFE-based strip offers a simple, rapid, sensitive, and accurate strategy for quantitative detection of AFB1 in soybean sauce.
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silver nanoparticle based Fluorescence Quenching lateral flow immunoassay for sensitive detection of ochratoxin a in grape juice and wine
Toxins, 2017Co-Authors: Hu Jiang, Lijuan Nie, Ying Xiong, Ke Pei, Yonghua XiongAbstract:A silver nanoparticle (AgNP)-based Fluorescence-Quenching lateral flow immunoassay with competitive format (cLFIA) was developed for sensitive detection of ochratoxin A (OTA) in grape juice and wine samples in the present study. The Ru(phen) 3 2 + -doped silica nanoparticles (RuNPs) were sprayed on the test and control line zones as background Fluorescence signals. The AgNPs were designed as the Fluorescence quenchers of RuNPs because they can block the exciting light transferring to the RuNP molecules. The proposed method exhibited high sensitivity for OTA detection, with a detection limit of 0.06 µg/L under optimized conditions. The method also exhibited a good linear range for OTA quantitative analysis from 0.08 µg/L to 5.0 µg/L. The reliability of the Fluorescence-Quenching cLFIA method was evaluated through analysis of the OTA-spiked red grape wine and juice samples. The average recoveries ranged from 88.0% to 110.0% in red grape wine and from 92.0% to 110.0% in grape juice. Meanwhile, less than a 10% coefficient variation indicated an acceptable precision of the cLFIA method. In summary, the new AgNP-based Fluorescence-Quenching cLFIA is a simple, rapid, sensitive, and accurate method for quantitative detection of OTA in grape juice and wine or other foodstuffs.
Jaemyung Kim - One of the best experts on this subject based on the ideXlab platform.
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Visualizing graphene based sheets by Fluorescence Quenching microscopy
Journal of the American Chemical Society, 2010Co-Authors: Jaemyung Kim, Laura J. Cote, Franklin Kim, Jiaxing HuangAbstract:Graphene based sheets have stimulated great interest due to their superior mechanical, electrical, and thermal properties. A general visualization method that allows quick observation of these single atomic layers would be highly desirable as it can greatly facilitate sample evaluation and manipulation, and provide immediate feedback to improve synthesis and processing strategies. Here we report that graphene based sheets can be made highly visible under a Fluorescence microscope by Quenching the emission from a dye coating, which can be conveniently removed afterward by rinsing without disrupting the sheets. Current imaging techniques for graphene based sheets rely on the use of special substrates. In contrast, the Fluorescence Quenching mechanism is no longer limited by the type of substrate. Graphene, reduced graphene oxide, or even graphene oxide sheets deposited on arbitrary substrates can now be readily visualized with good contrast for layer counting. Direct observation of suspended sheets in solution was also demonstrated. The Fluorescence Quenching microscopy offers unprecedented imaging flexibility and could become a general tool for characterizing graphene based materials.
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visualizing graphene based sheets by Fluorescence Quenching microscopy
arXiv: Materials Science, 2009Co-Authors: Jaemyung Kim, Laura J. Cote, Franklin Kim, Jiaxing HuangAbstract:Graphene based sheets have stimulated great interest due to their superior mechanical, electrical and thermal properties. A general visualization method that allows quick observation of these single atomic layers would be highly desirable as it can greatly facilitate sample evaluation and manipulation, and provide immediate feedback to improve synthesis and processing strategies. Here we report that graphene based sheets can be made highly visible under a Fluorescence microscope by Quenching the emission from a dye coating, which can be conveniently removed afterwards by rinsing without disrupting the sheets. Current imaging techniques for graphene based sheets rely on the use of special substrates. In contrast, the Fluorescence Quenching mechanism is no longer limited by the types of substrates. Graphene, reduced graphene oxide, or even graphene oxide sheets deposited on arbitrary substrates can now be readily visualized by eye with good contrast for layer counting. Direct observation of suspended sheets in solution was also demonstrated. The Fluorescence Quenching microscopy offers unprecedented imaging flexibility and could become a general tool for characterizing graphene based materials.