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William Shu-biu Yeung - One of the best experts on this subject based on the ideXlab platform.

  • Cumulus cells reduce the spermatozoa-zona binding inhibitory activity of human Follicular Fluid.
    Fertility and sterility, 2003
    Co-Authors: Shun-jia Hong, Jenny Y M Tse, William Shu-biu Yeung
    Abstract:

    To investigate the effects of human Follicular Fluid cultured with cumulus cells to inhibit the binding of spermatozoa to the zona pellucida of oocytes. Controlled experimental laboratory study. University gynecology unit. Women undergoing assisted reproduction program and men visiting the subfertility clinics. Culture medium and human Follicular Fluid were used to culture cumulus cells in vitro for specified time periods. Zona binding capacity and motility of spermatozoa after incubation with cumulus cells treated culture medium or human Follicular Fluid. Compared with the control medium, spent culture media after culturing cumulus cells for 3, 5, and 7 hours did not affect the motility and zona binding capacity of the treated spermatozoa. Significantly more spermatozoa treated with human Follicular Fluid that had been preincubated with cumulus cells for 5 and 7 hours bound onto hemizona in hemizona binding assay when compared with those preincubated in human Follicular Fluid without cumulus treatment. The hemizona index increased with the increase in the duration of cumulus cell treatment. Human Follicular Fluid with or without cumulus cells maintained sperm motility to similar extent for 3 hours. Cumulus cells reduced the inhibitory effect of human Follicular Fluid on spermatozoa-zona binding in vitro in a time-dependent manner.

  • Cumulus cells reduce the spermatozoa-zona binding inhibitory activity of human Follicular Fluid
    Fertility and Sterility, 2003
    Co-Authors: Shun-jia Hong, Jenny Y M Tse, William Shu-biu Yeung
    Abstract:

    Abstract Objective To investigate the effects of human Follicular Fluid cultured with cumulus cells to inhibit the binding of spermatozoa to the zona pellucida of oocytes. Design Controlled experimental laboratory study. Setting University gynecology unit. Patient(s) Women undergoing assisted reproduction program and men visiting the subfertility clinics. Intervention(s) Culture medium and human Follicular Fluid were used to culture cumulus cells in vitro for specified time periods. Main outcome measure(s) Zona binding capacity and motility of spermatozoa after incubation with cumulus cells treated culture medium or human Follicular Fluid. Result(s) Compared with the control medium, spent culture media after culturing cumulus cells for 3, 5, and 7 hours did not affect the motility and zona binding capacity of the treated spermatozoa. Significantly more spermatozoa treated with human Follicular Fluid that had been preincubated with cumulus cells for 5 and 7 hours bound onto hemizona in hemizona binding assay when compared with those preincubated in human Follicular Fluid without cumulus treatment. The hemizona index increased with the increase in the duration of cumulus cell treatment. Human Follicular Fluid with or without cumulus cells maintained sperm motility to similar extent for 3 hours. Conclusion(s) Cumulus cells reduced the inhibitory effect of human Follicular Fluid on spermatozoa–zona binding in vitro in a time-dependent manner.

Shun-jia Hong - One of the best experts on this subject based on the ideXlab platform.

  • Cumulus cells reduce the spermatozoa-zona binding inhibitory activity of human Follicular Fluid.
    Fertility and sterility, 2003
    Co-Authors: Shun-jia Hong, Jenny Y M Tse, William Shu-biu Yeung
    Abstract:

    To investigate the effects of human Follicular Fluid cultured with cumulus cells to inhibit the binding of spermatozoa to the zona pellucida of oocytes. Controlled experimental laboratory study. University gynecology unit. Women undergoing assisted reproduction program and men visiting the subfertility clinics. Culture medium and human Follicular Fluid were used to culture cumulus cells in vitro for specified time periods. Zona binding capacity and motility of spermatozoa after incubation with cumulus cells treated culture medium or human Follicular Fluid. Compared with the control medium, spent culture media after culturing cumulus cells for 3, 5, and 7 hours did not affect the motility and zona binding capacity of the treated spermatozoa. Significantly more spermatozoa treated with human Follicular Fluid that had been preincubated with cumulus cells for 5 and 7 hours bound onto hemizona in hemizona binding assay when compared with those preincubated in human Follicular Fluid without cumulus treatment. The hemizona index increased with the increase in the duration of cumulus cell treatment. Human Follicular Fluid with or without cumulus cells maintained sperm motility to similar extent for 3 hours. Cumulus cells reduced the inhibitory effect of human Follicular Fluid on spermatozoa-zona binding in vitro in a time-dependent manner.

  • Cumulus cells reduce the spermatozoa-zona binding inhibitory activity of human Follicular Fluid
    Fertility and Sterility, 2003
    Co-Authors: Shun-jia Hong, Jenny Y M Tse, William Shu-biu Yeung
    Abstract:

    Abstract Objective To investigate the effects of human Follicular Fluid cultured with cumulus cells to inhibit the binding of spermatozoa to the zona pellucida of oocytes. Design Controlled experimental laboratory study. Setting University gynecology unit. Patient(s) Women undergoing assisted reproduction program and men visiting the subfertility clinics. Intervention(s) Culture medium and human Follicular Fluid were used to culture cumulus cells in vitro for specified time periods. Main outcome measure(s) Zona binding capacity and motility of spermatozoa after incubation with cumulus cells treated culture medium or human Follicular Fluid. Result(s) Compared with the control medium, spent culture media after culturing cumulus cells for 3, 5, and 7 hours did not affect the motility and zona binding capacity of the treated spermatozoa. Significantly more spermatozoa treated with human Follicular Fluid that had been preincubated with cumulus cells for 5 and 7 hours bound onto hemizona in hemizona binding assay when compared with those preincubated in human Follicular Fluid without cumulus treatment. The hemizona index increased with the increase in the duration of cumulus cell treatment. Human Follicular Fluid with or without cumulus cells maintained sperm motility to similar extent for 3 hours. Conclusion(s) Cumulus cells reduced the inhibitory effect of human Follicular Fluid on spermatozoa–zona binding in vitro in a time-dependent manner.

Jenny Y M Tse - One of the best experts on this subject based on the ideXlab platform.

  • Cumulus cells reduce the spermatozoa-zona binding inhibitory activity of human Follicular Fluid.
    Fertility and sterility, 2003
    Co-Authors: Shun-jia Hong, Jenny Y M Tse, William Shu-biu Yeung
    Abstract:

    To investigate the effects of human Follicular Fluid cultured with cumulus cells to inhibit the binding of spermatozoa to the zona pellucida of oocytes. Controlled experimental laboratory study. University gynecology unit. Women undergoing assisted reproduction program and men visiting the subfertility clinics. Culture medium and human Follicular Fluid were used to culture cumulus cells in vitro for specified time periods. Zona binding capacity and motility of spermatozoa after incubation with cumulus cells treated culture medium or human Follicular Fluid. Compared with the control medium, spent culture media after culturing cumulus cells for 3, 5, and 7 hours did not affect the motility and zona binding capacity of the treated spermatozoa. Significantly more spermatozoa treated with human Follicular Fluid that had been preincubated with cumulus cells for 5 and 7 hours bound onto hemizona in hemizona binding assay when compared with those preincubated in human Follicular Fluid without cumulus treatment. The hemizona index increased with the increase in the duration of cumulus cell treatment. Human Follicular Fluid with or without cumulus cells maintained sperm motility to similar extent for 3 hours. Cumulus cells reduced the inhibitory effect of human Follicular Fluid on spermatozoa-zona binding in vitro in a time-dependent manner.

  • Cumulus cells reduce the spermatozoa-zona binding inhibitory activity of human Follicular Fluid
    Fertility and Sterility, 2003
    Co-Authors: Shun-jia Hong, Jenny Y M Tse, William Shu-biu Yeung
    Abstract:

    Abstract Objective To investigate the effects of human Follicular Fluid cultured with cumulus cells to inhibit the binding of spermatozoa to the zona pellucida of oocytes. Design Controlled experimental laboratory study. Setting University gynecology unit. Patient(s) Women undergoing assisted reproduction program and men visiting the subfertility clinics. Intervention(s) Culture medium and human Follicular Fluid were used to culture cumulus cells in vitro for specified time periods. Main outcome measure(s) Zona binding capacity and motility of spermatozoa after incubation with cumulus cells treated culture medium or human Follicular Fluid. Result(s) Compared with the control medium, spent culture media after culturing cumulus cells for 3, 5, and 7 hours did not affect the motility and zona binding capacity of the treated spermatozoa. Significantly more spermatozoa treated with human Follicular Fluid that had been preincubated with cumulus cells for 5 and 7 hours bound onto hemizona in hemizona binding assay when compared with those preincubated in human Follicular Fluid without cumulus treatment. The hemizona index increased with the increase in the duration of cumulus cell treatment. Human Follicular Fluid with or without cumulus cells maintained sperm motility to similar extent for 3 hours. Conclusion(s) Cumulus cells reduced the inhibitory effect of human Follicular Fluid on spermatozoa–zona binding in vitro in a time-dependent manner.

Katherine E Williams - One of the best experts on this subject based on the ideXlab platform.

  • Proteomic analysis of human Follicular Fluid from fertile women
    Clinical Proteomics, 2015
    Co-Authors: Alberuni M Zamah, Maria E Hassis, Matthew E Albertolle, Katherine E Williams
    Abstract:

    Background Follicular Fluid is a unique biological Fluid in which the critical events of oocyte and Follicular maturation and somatic cell-germ cell communication occur. Because of the intimate proximity of Follicular Fluid to the maturing oocyte, this Fluid provides a unique window into the processes occurring during Follicular maturation. A thorough identification of the specific components within Follicular Fluid may provide a better understanding of intraFollicular signaling, as well as reveal potential biomarkers of oocyte health for women undergoing assisted reproductive treatment. In this study, we used high and low pH HPLC peptide separations followed by mass spectrometry to perform a comprehensive proteomic analysis of human Follicular Fluid from healthy ovum donors. Next, using samples from a second set of patients, an isobaric mass tagging strategy for quantitative analysis was used to identify proteins with altered abundances after hCG treatment. Results A total of 742 Follicular Fluid proteins were identified in healthy ovum donors, including 413 that have not been previously reported. The proteins belong to diverse functional groups including insulin growth factor and insulin growth factor binding protein families, growth factor and related proteins, receptor signaling, defense/immunity, anti-apoptotic proteins, matrix metalloprotease related proteins, and complement activity. In a quantitative analysis, Follicular Fluid samples from age-matched women undergoing in vitro fertilization oocyte retrieval were compared and 17 Follicular Fluid proteins were found at significantly altered levels (p 

  • Proteomic analysis of human Follicular Fluid from fertile women
    Clinical proteomics, 2015
    Co-Authors: Alberuni M Zamah, Maria E Hassis, Matthew E Albertolle, Katherine E Williams
    Abstract:

    Background Follicular Fluid is a unique biological Fluid in which the critical events of oocyte and Follicular maturation and somatic cell-germ cell communication occur. Because of the intimate proximity of Follicular Fluid to the maturing oocyte, this Fluid provides a unique window into the processes occurring during Follicular maturation. A thorough identification of the specific components within Follicular Fluid may provide a better understanding of intraFollicular signaling, as well as reveal potential biomarkers of oocyte health for women undergoing assisted reproductive treatment. In this study, we used high and low pH HPLC peptide separations followed by mass spectrometry to perform a comprehensive proteomic analysis of human Follicular Fluid from healthy ovum donors. Next, using samples from a second set of patients, an isobaric mass tagging strategy for quantitative analysis was used to identify proteins with altered abundances after hCG treatment.

Alberuni M Zamah - One of the best experts on this subject based on the ideXlab platform.

  • Proteomic analysis of human Follicular Fluid from fertile women
    Clinical Proteomics, 2015
    Co-Authors: Alberuni M Zamah, Maria E Hassis, Matthew E Albertolle, Katherine E Williams
    Abstract:

    Background Follicular Fluid is a unique biological Fluid in which the critical events of oocyte and Follicular maturation and somatic cell-germ cell communication occur. Because of the intimate proximity of Follicular Fluid to the maturing oocyte, this Fluid provides a unique window into the processes occurring during Follicular maturation. A thorough identification of the specific components within Follicular Fluid may provide a better understanding of intraFollicular signaling, as well as reveal potential biomarkers of oocyte health for women undergoing assisted reproductive treatment. In this study, we used high and low pH HPLC peptide separations followed by mass spectrometry to perform a comprehensive proteomic analysis of human Follicular Fluid from healthy ovum donors. Next, using samples from a second set of patients, an isobaric mass tagging strategy for quantitative analysis was used to identify proteins with altered abundances after hCG treatment. Results A total of 742 Follicular Fluid proteins were identified in healthy ovum donors, including 413 that have not been previously reported. The proteins belong to diverse functional groups including insulin growth factor and insulin growth factor binding protein families, growth factor and related proteins, receptor signaling, defense/immunity, anti-apoptotic proteins, matrix metalloprotease related proteins, and complement activity. In a quantitative analysis, Follicular Fluid samples from age-matched women undergoing in vitro fertilization oocyte retrieval were compared and 17 Follicular Fluid proteins were found at significantly altered levels (p 

  • Proteomic analysis of human Follicular Fluid from fertile women
    Clinical proteomics, 2015
    Co-Authors: Alberuni M Zamah, Maria E Hassis, Matthew E Albertolle, Katherine E Williams
    Abstract:

    Background Follicular Fluid is a unique biological Fluid in which the critical events of oocyte and Follicular maturation and somatic cell-germ cell communication occur. Because of the intimate proximity of Follicular Fluid to the maturing oocyte, this Fluid provides a unique window into the processes occurring during Follicular maturation. A thorough identification of the specific components within Follicular Fluid may provide a better understanding of intraFollicular signaling, as well as reveal potential biomarkers of oocyte health for women undergoing assisted reproductive treatment. In this study, we used high and low pH HPLC peptide separations followed by mass spectrometry to perform a comprehensive proteomic analysis of human Follicular Fluid from healthy ovum donors. Next, using samples from a second set of patients, an isobaric mass tagging strategy for quantitative analysis was used to identify proteins with altered abundances after hCG treatment.