The Experts below are selected from a list of 645 Experts worldwide ranked by ideXlab platform
Cifuentes Alejandro - One of the best experts on this subject based on the ideXlab platform.
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Foodomics of bioactive compounds from tropical fruits by-products
'Elsevier BV', 2021Co-Authors: Álvarez-rivera Gerardo, Ballesteros-vivas Diego, Ibáñez Elena, Parada-alfonso Fabián, Cifuentes AlejandroAbstract:Tropical fruit by-products can be considered a reservoir of bioactive substances with promising benefits on human's health. In recent years, the recovery of bioactive compounds from agro-food by-products is an emerging trend in food science that largely contributes to the valorization of food industrial processing generated wastes. In this chapter, a general overview about the potential of tropical fruits by-products as sources of bioactive compounds will be provided under a Foodomics perspective. The integration of advanced extraction procedures, along with multi-analytical and multi-omics platforms for the development of tropical fruit by-products valorization strategies are reviewed in this chapter.Peer reviewe
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Foodomics – Fundamentals, State of the Art and Future Trends
'Royal Society of Chemistry (RSC)', 2021Co-Authors: Álvarez-rivera Gerardo, León Carlos, Valdés Alberto, Cifuentes AlejandroAbstract:Foodomics is being consolidated in food science through the application and integration of a variety of omics tools (e.g., genomics, transcriptomics, proteomics, metabolomics) together with chemometrics and bioinformatics. Foodomics can greatly improve our understanding of the complex food–diet–individual interplay, involving different food science and nutrition research areas dealing with food composition, food safety, quality and traceability issues, as well as the effects of food on an individual's health or illness status. Readers of this chapter will get an overview of the fundamentals, the most recent advances and future perspectives in the different areas of Foodomics.Peer reviewe
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Foodomics evaluation of genetically modified organisms
'Elsevier BV', 2020Co-Authors: León Carlos, Cifuentes AlejandroAbstract:Second Edition. Chapter 15.A transgenic food is defined as a food that is derived from or contains genetically modified organisms (GMOs). A GMO is formed by the insertion of one or more functional genes into the genome of another organism by means of genetic engineering (also termed as recombinant DNA technology). The characterization, detection, and quantitation of these new transgenic products have brought about a considerable demand for new and more powerful analytical methods able to analyze GMOs along the food chain. This chapter is an update of the work published in 2012 by our group in the book: Chemical Analysis of Food: Techniques & Applications, edited by Y. Pico (Elsevier, Amsterdam). This chapter discusses recent developments and future outlooks in the characterization and detection of GMOs in foods. Namely, the chapter describes the different omics methodologies proposed so far based on untargeted profiling or target analysis to analyze GMOs. Advantages and limitations derived from the application of these Foodomics methodologies are highlighted and discussed.Peer reviewe
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Ten years on the Foodomics' road ... and counting
2020Co-Authors: Álvarez-rivera Gerardo, Ballesteros-vivas Diego, Ibáñez Elena, Valdés Alberto, Herrero Miguel, Mendiola J. A., Suárez Montenegro Z., Sánchez-martínez J. David, Cifuentes AlejandroAbstract:Trabajo presentado al 6th edition of the International Conference on Foodomics, celebrada en Cesena (Italy) del 14 al 16 de octubre de 2020.Peer reviewe
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Anti-proliferative bioactivity against HT-29 colon cancer cells of a withanolides-rich extract from golden berry (Physalis peruviana L.) calyx investigated by Foodomics
'Elsevier BV', 2019Co-Authors: Ballesteros-vivas Diego, Álvarez-rivera Gerardo, León Carlos, Morantes, Sandra Johanna, Ibáñez Elena, Parada-alfonso Fabián, Cifuentes Alejandro, Valdés AlbertoAbstract:A comprehensive Foodomics study was carried out in this work to investigate the changes induced at gene and metabolite expression levels on HT29 colon cancer cell lines upon treatment with a bioactives-enriched extract from goldenberry calyx. As a result of the proposed multi-omics approach, the tested extract induced transcriptional activation of pro-apoptotic genes, and altered the expression of several genes related to the oxidative stress response in treated cancer cells. Metabolomics data confirm altered cellular redox homeostasis, providing additional evidence to transcriptomic results. Foodomics data integration also revealed alteration on relevant metabolic processes, suggesting inactivation of aminoacyl tRNA charging pathway, dysfunction on carnitine shuttle and beta-oxidation of fatty acids, and pyrimidine ribonucleotide interconversion impairment. These observations are in line with functional analysis and anti-proliferative activity results, where the viability of HT-29 colon cancer cells was notably reduced after 48 h treatment without affecting the viability of normal human colon fibroblast cells.This research was supported by COOPA20145, project from CSIC (Programa de Cooperación Científica para el Desarrollo “i-COOP+”). G.A.-R. and A.V. would like to acknowledge the Ministry of Economy and Competitiveness for a “Juan de la Cierva” postdoctoral grant. The authors also thank the support from the AGL2017-89417-R project.Peer reviewe
Valdés Alberto - One of the best experts on this subject based on the ideXlab platform.
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Foodomics – Fundamentals, State of the Art and Future Trends
'Royal Society of Chemistry (RSC)', 2021Co-Authors: Álvarez-rivera Gerardo, León Carlos, Valdés Alberto, Cifuentes AlejandroAbstract:Foodomics is being consolidated in food science through the application and integration of a variety of omics tools (e.g., genomics, transcriptomics, proteomics, metabolomics) together with chemometrics and bioinformatics. Foodomics can greatly improve our understanding of the complex food–diet–individual interplay, involving different food science and nutrition research areas dealing with food composition, food safety, quality and traceability issues, as well as the effects of food on an individual's health or illness status. Readers of this chapter will get an overview of the fundamentals, the most recent advances and future perspectives in the different areas of Foodomics.Peer reviewe
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Ten years on the Foodomics' road ... and counting
2020Co-Authors: Álvarez-rivera Gerardo, Ballesteros-vivas Diego, Ibáñez Elena, Valdés Alberto, Herrero Miguel, Mendiola J. A., Suárez Montenegro Z., Sánchez-martínez J. David, Cifuentes AlejandroAbstract:Trabajo presentado al 6th edition of the International Conference on Foodomics, celebrada en Cesena (Italy) del 14 al 16 de octubre de 2020.Peer reviewe
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Anti-proliferative bioactivity against HT-29 colon cancer cells of a withanolides-rich extract from golden berry (Physalis peruviana L.) calyx investigated by Foodomics
'Elsevier BV', 2019Co-Authors: Ballesteros-vivas Diego, Álvarez-rivera Gerardo, León Carlos, Morantes, Sandra Johanna, Ibáñez Elena, Parada-alfonso Fabián, Cifuentes Alejandro, Valdés AlbertoAbstract:A comprehensive Foodomics study was carried out in this work to investigate the changes induced at gene and metabolite expression levels on HT29 colon cancer cell lines upon treatment with a bioactives-enriched extract from goldenberry calyx. As a result of the proposed multi-omics approach, the tested extract induced transcriptional activation of pro-apoptotic genes, and altered the expression of several genes related to the oxidative stress response in treated cancer cells. Metabolomics data confirm altered cellular redox homeostasis, providing additional evidence to transcriptomic results. Foodomics data integration also revealed alteration on relevant metabolic processes, suggesting inactivation of aminoacyl tRNA charging pathway, dysfunction on carnitine shuttle and beta-oxidation of fatty acids, and pyrimidine ribonucleotide interconversion impairment. These observations are in line with functional analysis and anti-proliferative activity results, where the viability of HT-29 colon cancer cells was notably reduced after 48 h treatment without affecting the viability of normal human colon fibroblast cells.This research was supported by COOPA20145, project from CSIC (Programa de Cooperación Científica para el Desarrollo “i-COOP+”). G.A.-R. and A.V. would like to acknowledge the Ministry of Economy and Competitiveness for a “Juan de la Cierva” postdoctoral grant. The authors also thank the support from the AGL2017-89417-R project.Peer reviewe
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Foodomics: Ten years on the road
'Japanese Society of Applied Entomology & Zoology', 2019Co-Authors: Álvarez-rivera Gerardo, Ballesteros-vivas Diego, Ibáñez Elena, Valdés Alberto, Herrero Miguel, Mendiola J. A., Suárez Montenegro Z., Sánchez-martínez J. David, Cifuentes AlejandroAbstract:Resumen del trabajo presentado al 25th Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology (LACE), celebrado en Alcalá de Henares, Madrid (España) del 29 de septiembre al 2 de octubre de 2019.In the last ten years, the main line of research in our lab has focused on obtaining bioactive compounds using green pressurized extraction. These were subjected to a Foodomics evaluation of their activities against different high incidence diseases as colon cancer and Alzheimer’s disease. In this work, we will present some selected results on these topics including results from: a) the development of new green extraction processes to obtain bioactive compounds from different natural sources (algae, microalgae, food by-products, plants, etc.); b) the determination of the bioactivity of the new extracts against different colon cancer in-vitro and in-vivo models and Alzheimer in-vitro models; c) the development of advanced analytical approaches including metabolomics profiling based on LC-MS, CE-MS, GC-MS and comprehensive LCxLC-MS/MS for the chemical characterization of the bioactive extracts; d) the identification of genes, proteins and metabolites differentially expressed in cancer cells using whole-transcriptome microarrays, nano-LC-MS for proteomics and/or non-targeted whole-metabolome approaches based on LC-MS and CE-MS and; e) the development of algorithms for the analysis of these MS-based datasets. This work is an excellent example of the important challenges that still have to be addressed by Foodomics to solve the binomial Food & Health dependency and it will allow us to discuss some of the current and future challenges in this area of research.This work has been sponsored by AGL2017-89417-R and ABACUS-H2020 projects.Peer reviewe
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Chromatography and Foodomics: the perfect pairing
2019Co-Authors: Álvarez-rivera Gerardo, Ballesteros-vivas Diego, León Carlos, Morantes, Sandra Johanna, Ibáñez Elena, Parada-alfonso Fabián, Valdés Alberto, Cifuentes AlejandroAbstract:Resumen del trabajo presentado al XVII Latin American Symposium on Chromatography and Related Techniques (COLACRO), celebrado en Aracaju/SE (Brasil) del 14 al 19 de julio de 2019.Chromatography plays a crucial role in a huge number of areas and applications. In this work, we will show how chromatography is helping to the development of a new scientific discipline as Foodomics, through a case study focused on food by-products valorisation and colon cancer. The valorisation of food industry by-products is an emerging trend in food science that has shown to be a valuable alternative to extract natural compounds with added value and demonstrated bioactivity from food wastes, which are usually discarded or employed as animal feed. Most of the reported studies in literature about tropical fruits are focused on the fruit itself, whereas the identification of bioactive substances of by-products from tropical fruits is still scarce or has not been done yet. Considering the potential of tropical fruits by-products as promising source of bioactive phytochemicals, a complete valorization strategy based on green Foodomics is presented in this work, investigating Physalis peruviana L. calyces, Mangifera indica L. seeds kernels and Passiflora mollisima seeds The results include pressurized liquid extraction (PLE) of the bioactive compounds, followed by a comprehensive analysis of the compounds extracted by LC and GC coupled to quadrupole time-of-flight tandem mass spectrometry (Q-TOF-MS/MS), applying integrated identification approaches for the confident identification and structural elucidation of phytochemicals. The bioactivity of the extracts was investigated using in vitro assays against human colon cancer cells and their action mechanisms explored through metabolomic and transcriptomic approaches. Moreover, the low toxicity of the most active extract was also confirmed. The proposed strategy can be implemented for the complete characterization of preparations with health-promoting effects, intended to be used as functional foods or nutraceuticals, and in traditional medicine.Peer reviewe
Alejandro Cifuentes - One of the best experts on this subject based on the ideXlab platform.
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Anti-proliferative bioactivity against HT-29 colon cancer cells of a withanolides-rich extract from golden berry (Physalis peruviana L.) calyx investigated by Foodomics
Journal of Functional Foods, 2019Co-Authors: Diego Ballesteros-vivas, Gerardo Álvarez-rivera, Carlos León, Sandra Johanna Morantes, Elena Ibáñez, Fabián Parada-alfonso, Alejandro Cifuentes, Alberto ValdésAbstract:Abstract A comprehensive Foodomics study was carried out in this work to investigate the changes induced at gene and metabolite expression levels on HT29 colon cancer cell lines upon treatment with a bioactives-enriched extract from goldenberry calyx. As a result of the proposed multi-omics approach, the tested extract induced transcriptional activation of pro-apoptotic genes, and altered the expression of several genes related to the oxidative stress response in treated cancer cells. Metabolomics data confirm altered cellular redox homeostasis, providing additional evidence to transcriptomic results. Foodomics data integration also revealed alteration on relevant metabolic processes, suggesting inactivation of aminoacyl tRNA charging pathway, dysfunction on carnitine shuttle and beta-oxidation of fatty acids, and pyrimidine ribonucleotide interconversion impairment. These observations are in line with functional analysis and anti-proliferative activity results, where the viability of HT-29 colon cancer cells was notably reduced after 48 h treatment without affecting the viability of normal human colon fibroblast cells.
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Comprehensive Foodomics Study on the Mechanisms Operating at Various Molecular Levels in Cancer Cells in Response to Individual Rosemary Polyphenols
2014Co-Authors: Alberto Valdés, Virginia García-cañas, Carolina Simó, Clara Ibáñez, Vicente Micol, Jose A. Ferragut, Alejandro CifuentesAbstract:In this work, the contribution of carnosic acid (CA) and carnosol (CS), two major compounds present in rosemary, against colon cancer HT-29 cells proliferation is investigated using a comprehensive Foodomics approach. The Foodomics study reveals that CA induces transcriptional activation of genes that encode detoxifying enzymes and altered the expression of genes linked to transport and biosynthesis of terpenoids in the colon cancer cell line. Functional analysis highlighted the activation of the ROS metabolism and alteration of several genes involved in pathways describing oxidative degradation of relevant endogenous metabolites, providing new evidence about the transcriptional change induced by CA in HT-29 cells. Metabolomics analysis showed that the treatment with CA affected the intracellular levels of glutathione. Elevated levels of GSH provided additional evidence to transcriptomic results regarding chemopreventive response of cells to CA treatment. Moreover, the Foodomics approach was useful to establish the links between decreased levels of N-acetylputrescine and its degradation pathway at the gene level. The findings from this work and the predictions based on microarray data will help explore novel metabolic processes and potential signaling pathways to further elucidate the effect of CA in colon cancer cells
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miracles multi product integrated biorefinery of algae from carbon dioxide and light energy to high value specialties
2014Co-Authors: Miguel Herrero, Alejandro Cifuentes, J A Mendiola, Elena IbáñezAbstract:Trabajo presentado a las I Jornadas Cientificas del CIAL celebradas el 5 de junio de 2014 en Madrid.-- MIRACLES es un proyecto de innovacion con un enfoque multidisciplinar orientado a la generacion de casos de negocio solidos a traves del desarrollo de tecnologia de la industria impulsada por la I+D. El consorcio cuenta con 26 socios de 11 destacadas organizaciones de investigacion. Entre ellos el CIAL esta representado a traves del grupo Foodomics. El liderazgo industrial fuerte esta garantizado a traves de la participacion de 12 PYMES y 3 usuarios finales. http://miraclesproject.eu/ (KBBE.2013.3.2-02: The CO2 algae biorefinery)
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Foodomics ms based strategies in modern food science and nutrition
Mass Spectrometry Reviews, 2012Co-Authors: Miguel Herrero, Elena Ibáñez, Carolina Simo, Virginia Garciacanas, Alejandro CifuentesAbstract:Modern research in food science and nutrition is moving from classical methodologies to advanced analytical strategies in which MS-based techniques play a crucial role. In this context, Foodomics has been recently defined as a new discipline that studies food and nutrition domains through the application of advanced omics technologies in which MS techniques are considered indispensable. Applications of Foodomics include the genomic, transcriptomic, proteomic, and/or metabolomic study of foods for compound profiling, authenticity, and/or biomarker-detection related to food quality or safety; the development of new transgenic foods, food contaminants, and whole toxicity studies; new investigations on food bioactivity, food effects on human health, etc. This review work does not intend to provide an exhaustive revision of the many works published so far on food analysis using MS techniques. The aim of the present work is to provide an overview of the different MS-based strategies that have been (or can be) applied in the new field of Foodomics, discussing their advantages and drawbacks. Besides, some ideas about the foreseen development and applications of MS-techniques in this new discipline are also provided. © 2011 Wiley Periodicals, Inc., Mass Spec Rev 31:49–69, 2012
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Foodomics ms based strategies in modern food science and nutrition
Mass Spectrometry Reviews, 2012Co-Authors: Miguel Herrero, Elena Ibáñez, Carolina Simo, Virginia Garciacanas, Alejandro CifuentesAbstract:Modern research in food science and nutrition is moving from classical methodologies to advanced analytical strategies in which MS-based techniques play a crucial role. In this context, Foodomics has been recently defined as a new discipline that studies food and nutrition domains through the application of advanced omics technologies in which MS techniques are considered indispensable. Applications of Foodomics include the genomic, transcriptomic, proteomic, and/or metabolomic study of foods for compound profiling, authenticity, and/or biomarker-detection related to food quality or safety; the development of new transgenic foods, food contaminants, and whole toxicity studies; new investigations on food bioactivity, food effects on human health, etc. This review work does not intend to provide an exhaustive revision of the many works published so far on food analysis using MS techniques. The aim of the present work is to provide an overview of the different MS-based strategies that have been (or can be) applied in the new field of Foodomics, discussing their advantages and drawbacks. Besides, some ideas about the foreseen development and applications of MS-techniques in this new discipline are also provided.
Ibáñez Elena - One of the best experts on this subject based on the ideXlab platform.
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Foodomics of bioactive compounds from tropical fruits by-products
'Elsevier BV', 2021Co-Authors: Álvarez-rivera Gerardo, Ballesteros-vivas Diego, Ibáñez Elena, Parada-alfonso Fabián, Cifuentes AlejandroAbstract:Tropical fruit by-products can be considered a reservoir of bioactive substances with promising benefits on human's health. In recent years, the recovery of bioactive compounds from agro-food by-products is an emerging trend in food science that largely contributes to the valorization of food industrial processing generated wastes. In this chapter, a general overview about the potential of tropical fruits by-products as sources of bioactive compounds will be provided under a Foodomics perspective. The integration of advanced extraction procedures, along with multi-analytical and multi-omics platforms for the development of tropical fruit by-products valorization strategies are reviewed in this chapter.Peer reviewe
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Ten years on the Foodomics' road ... and counting
2020Co-Authors: Álvarez-rivera Gerardo, Ballesteros-vivas Diego, Ibáñez Elena, Valdés Alberto, Herrero Miguel, Mendiola J. A., Suárez Montenegro Z., Sánchez-martínez J. David, Cifuentes AlejandroAbstract:Trabajo presentado al 6th edition of the International Conference on Foodomics, celebrada en Cesena (Italy) del 14 al 16 de octubre de 2020.Peer reviewe
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Anti-proliferative bioactivity against HT-29 colon cancer cells of a withanolides-rich extract from golden berry (Physalis peruviana L.) calyx investigated by Foodomics
'Elsevier BV', 2019Co-Authors: Ballesteros-vivas Diego, Álvarez-rivera Gerardo, León Carlos, Morantes, Sandra Johanna, Ibáñez Elena, Parada-alfonso Fabián, Cifuentes Alejandro, Valdés AlbertoAbstract:A comprehensive Foodomics study was carried out in this work to investigate the changes induced at gene and metabolite expression levels on HT29 colon cancer cell lines upon treatment with a bioactives-enriched extract from goldenberry calyx. As a result of the proposed multi-omics approach, the tested extract induced transcriptional activation of pro-apoptotic genes, and altered the expression of several genes related to the oxidative stress response in treated cancer cells. Metabolomics data confirm altered cellular redox homeostasis, providing additional evidence to transcriptomic results. Foodomics data integration also revealed alteration on relevant metabolic processes, suggesting inactivation of aminoacyl tRNA charging pathway, dysfunction on carnitine shuttle and beta-oxidation of fatty acids, and pyrimidine ribonucleotide interconversion impairment. These observations are in line with functional analysis and anti-proliferative activity results, where the viability of HT-29 colon cancer cells was notably reduced after 48 h treatment without affecting the viability of normal human colon fibroblast cells.This research was supported by COOPA20145, project from CSIC (Programa de Cooperación Científica para el Desarrollo “i-COOP+”). G.A.-R. and A.V. would like to acknowledge the Ministry of Economy and Competitiveness for a “Juan de la Cierva” postdoctoral grant. The authors also thank the support from the AGL2017-89417-R project.Peer reviewe
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Green Foodomics: new strategies for greener extraction techniques
2019Co-Authors: Herrero Miguel, Cifuentes Alejandro, Sánchez-camargo A. P., Montero Lidia, Mendiola J. A., Ibáñez ElenaAbstract:Resumen del trabajo presentado al XVII Latin American Symposium on Chromatography and Related Techniques (COLACRO), celebrado en Aracaju/SE (Brasil) del 14 al 19 de julio de 2019.Foodomics attempts to provide a global vision on the relationship between food and health through the use of -omics technologies and covers different fields of research included in food science and associated to sustainability. Green Foodomics follows up this approach while contributing to the greenness, sustainability and ecology of Foodomics as a whole. Among the different possibilities that can help Foodomics moving towards a cleaner scientific discipline, the replacement of toxic solvents for green solvents with lower environmental impact, the design of new biorefinery processes and the intensification and integration of processes are involved in both, the extraction of bioactives from natural sources (such as plants, algae, food by-products, among others) that can be used as food ingredients, and the necessary sample preparation steps to determine the chemical composition of the natural extracts (that should follow the green analytical chemistry (GAC) principles). In this presentation, different applications involving the development of green extraction methods (mainly based on compressed fluids) will be presented with the idea of moving from 'small to big' (or viceversa) for the extraction and characterization of bioactive compounds from natural sources.Peer reviewe
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Chromatography and Foodomics: the perfect pairing
2019Co-Authors: Álvarez-rivera Gerardo, Ballesteros-vivas Diego, León Carlos, Morantes, Sandra Johanna, Ibáñez Elena, Parada-alfonso Fabián, Valdés Alberto, Cifuentes AlejandroAbstract:Resumen del trabajo presentado al XVII Latin American Symposium on Chromatography and Related Techniques (COLACRO), celebrado en Aracaju/SE (Brasil) del 14 al 19 de julio de 2019.Chromatography plays a crucial role in a huge number of areas and applications. In this work, we will show how chromatography is helping to the development of a new scientific discipline as Foodomics, through a case study focused on food by-products valorisation and colon cancer. The valorisation of food industry by-products is an emerging trend in food science that has shown to be a valuable alternative to extract natural compounds with added value and demonstrated bioactivity from food wastes, which are usually discarded or employed as animal feed. Most of the reported studies in literature about tropical fruits are focused on the fruit itself, whereas the identification of bioactive substances of by-products from tropical fruits is still scarce or has not been done yet. Considering the potential of tropical fruits by-products as promising source of bioactive phytochemicals, a complete valorization strategy based on green Foodomics is presented in this work, investigating Physalis peruviana L. calyces, Mangifera indica L. seeds kernels and Passiflora mollisima seeds The results include pressurized liquid extraction (PLE) of the bioactive compounds, followed by a comprehensive analysis of the compounds extracted by LC and GC coupled to quadrupole time-of-flight tandem mass spectrometry (Q-TOF-MS/MS), applying integrated identification approaches for the confident identification and structural elucidation of phytochemicals. The bioactivity of the extracts was investigated using in vitro assays against human colon cancer cells and their action mechanisms explored through metabolomic and transcriptomic approaches. Moreover, the low toxicity of the most active extract was also confirmed. The proposed strategy can be implemented for the complete characterization of preparations with health-promoting effects, intended to be used as functional foods or nutraceuticals, and in traditional medicine.Peer reviewe
Elena Ibáñez - One of the best experts on this subject based on the ideXlab platform.
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Anti-proliferative bioactivity against HT-29 colon cancer cells of a withanolides-rich extract from golden berry (Physalis peruviana L.) calyx investigated by Foodomics
Journal of Functional Foods, 2019Co-Authors: Diego Ballesteros-vivas, Gerardo Álvarez-rivera, Carlos León, Sandra Johanna Morantes, Elena Ibáñez, Fabián Parada-alfonso, Alejandro Cifuentes, Alberto ValdésAbstract:Abstract A comprehensive Foodomics study was carried out in this work to investigate the changes induced at gene and metabolite expression levels on HT29 colon cancer cell lines upon treatment with a bioactives-enriched extract from goldenberry calyx. As a result of the proposed multi-omics approach, the tested extract induced transcriptional activation of pro-apoptotic genes, and altered the expression of several genes related to the oxidative stress response in treated cancer cells. Metabolomics data confirm altered cellular redox homeostasis, providing additional evidence to transcriptomic results. Foodomics data integration also revealed alteration on relevant metabolic processes, suggesting inactivation of aminoacyl tRNA charging pathway, dysfunction on carnitine shuttle and beta-oxidation of fatty acids, and pyrimidine ribonucleotide interconversion impairment. These observations are in line with functional analysis and anti-proliferative activity results, where the viability of HT-29 colon cancer cells was notably reduced after 48 h treatment without affecting the viability of normal human colon fibroblast cells.
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miracles multi product integrated biorefinery of algae from carbon dioxide and light energy to high value specialties
2014Co-Authors: Miguel Herrero, Alejandro Cifuentes, J A Mendiola, Elena IbáñezAbstract:Trabajo presentado a las I Jornadas Cientificas del CIAL celebradas el 5 de junio de 2014 en Madrid.-- MIRACLES es un proyecto de innovacion con un enfoque multidisciplinar orientado a la generacion de casos de negocio solidos a traves del desarrollo de tecnologia de la industria impulsada por la I+D. El consorcio cuenta con 26 socios de 11 destacadas organizaciones de investigacion. Entre ellos el CIAL esta representado a traves del grupo Foodomics. El liderazgo industrial fuerte esta garantizado a traves de la participacion de 12 PYMES y 3 usuarios finales. http://miraclesproject.eu/ (KBBE.2013.3.2-02: The CO2 algae biorefinery)
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Foodomics ms based strategies in modern food science and nutrition
Mass Spectrometry Reviews, 2012Co-Authors: Miguel Herrero, Elena Ibáñez, Carolina Simo, Virginia Garciacanas, Alejandro CifuentesAbstract:Modern research in food science and nutrition is moving from classical methodologies to advanced analytical strategies in which MS-based techniques play a crucial role. In this context, Foodomics has been recently defined as a new discipline that studies food and nutrition domains through the application of advanced omics technologies in which MS techniques are considered indispensable. Applications of Foodomics include the genomic, transcriptomic, proteomic, and/or metabolomic study of foods for compound profiling, authenticity, and/or biomarker-detection related to food quality or safety; the development of new transgenic foods, food contaminants, and whole toxicity studies; new investigations on food bioactivity, food effects on human health, etc. This review work does not intend to provide an exhaustive revision of the many works published so far on food analysis using MS techniques. The aim of the present work is to provide an overview of the different MS-based strategies that have been (or can be) applied in the new field of Foodomics, discussing their advantages and drawbacks. Besides, some ideas about the foreseen development and applications of MS-techniques in this new discipline are also provided. © 2011 Wiley Periodicals, Inc., Mass Spec Rev 31:49–69, 2012
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Foodomics ms based strategies in modern food science and nutrition
Mass Spectrometry Reviews, 2012Co-Authors: Miguel Herrero, Elena Ibáñez, Carolina Simo, Virginia Garciacanas, Alejandro CifuentesAbstract:Modern research in food science and nutrition is moving from classical methodologies to advanced analytical strategies in which MS-based techniques play a crucial role. In this context, Foodomics has been recently defined as a new discipline that studies food and nutrition domains through the application of advanced omics technologies in which MS techniques are considered indispensable. Applications of Foodomics include the genomic, transcriptomic, proteomic, and/or metabolomic study of foods for compound profiling, authenticity, and/or biomarker-detection related to food quality or safety; the development of new transgenic foods, food contaminants, and whole toxicity studies; new investigations on food bioactivity, food effects on human health, etc. This review work does not intend to provide an exhaustive revision of the many works published so far on food analysis using MS techniques. The aim of the present work is to provide an overview of the different MS-based strategies that have been (or can be) applied in the new field of Foodomics, discussing their advantages and drawbacks. Besides, some ideas about the foreseen development and applications of MS-techniques in this new discipline are also provided.