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Andrea Battistoni - One of the best experts on this subject based on the ideXlab platform.
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Correction: Zinc is required to ensure the expression of flagella and the ability to Form Biofilms in Salmonella enterica sv Typhimurium.
Metallomics : integrated biometal science, 2016Co-Authors: Serena Ammendola, Ylenia D'amico, Barbara Chirullo, Rosanna Drumo, Domenico Ciavardelli, Paolo Pasquali, Andrea BattistoniAbstract:Correction for 'Zinc is required to ensure the expression of flagella and the ability to Form Biofilms in Salmonella enterica sv Typhimurium' by Serena Ammendola et al., Metallomics, 2016, DOI: 10.1039/c6mt00108d.
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zinc is required to ensure the expression of flagella and the ability to Form Biofilms in salmonella enterica sv typhimurium
Metallomics, 2016Co-Authors: Serena Ammendola, Barbara Chirullo, Rosanna Drumo, Paolo Pasquali, Ylenia Damico, Domenico Civardelli, Andrea BattistoniAbstract:Zinc is known to play a central role in bacterial physiology and pathogenesis. Here, we report that the accumulation of FliC, the structural subunit of Salmonella phase 1 flagella, is sharply reduced in a znuABC Salmonella enterica sv. Typhimurium strain grown in zinc-poor media. Consequently, this mutant strain lacks motility, unless it grows in zinc-replete environments. This phenotype is the consequence of a general downregulation of all the genes involved in the biosynthesis of flagella, suggesting that zinc is the cofactor of proteins involved in the initiation of the transcriptional regulatory cascade leading to flagella assembly. Competition experiments in mice demonstrated that aflagellated (fliBfljC) and znuABC strains are outcompeted by the wild type strain in the gastrointestinal tract. The fliBfljC strain overgrows a fliCfljBznuABC mutant strain, but the difference in gut colonization between these two strains is less striking than that between the wild type and the znuABC strains, suggesting that the downregulation of flagella contributes to the loss of virulence of Salmonella znuABC. The absence of either flagella or ZnuABC also impairs the ability of S. Typhimurium to produce Biofilms. Zinc suppresses this defect in the znuABC mutant but not in the aflagellated strains, highlighting the role of flagella in biofilm organization. We have also observed an increased production of the quorum sensing signal AI-2 in the znuABC strain sensing zinc deprivation, that may further contribute to the reduced ability to Form Biofilms. On the whole, our study reveals novel roles of zinc in Salmonella motility and intercellular communication.
C W Keevil - One of the best experts on this subject based on the ideXlab platform.
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listeria monocytogenes can Form Biofilms in tap water and enter into the viable but non cultivable state
Microbial Ecology, 2014Co-Authors: M S Giao, C W KeevilAbstract:Listeria monocytogenes is a foodborne pathogen that can be transmitted through contaminated raw food or by ready-to-eat products that have been in contact with contaminated surfaces. Tap water (TW) is used to wash produce, as a processed food constituent and to wash processing surfaces and floors. The main aim of this work was to investigate the Formation and survival of L. monocytogenes Biofilms on stainless steel (SS) coupons in TW at 4, 22, 30 and 37 °C. For that, coupons with biofilm were visualised in situ while other coupons were scraped to quantify total cells by SYTO 9, cultivable numbers by plating onto brain heart infusion agar and viable numbers by the direct viable count method. Results showed that L. monocytogenes can Form Biofilms on SS surfaces in TW at any temperature, including at 4 °C. The number of total cells was similar for all the conditions tested while cultivable numbers varied between the level of detection (<8.3 CFU cm−2) and 3.5 × 105 CFU cm−2, meaning between 7.0 × 104 and 1.1 × 107 cells cm−2 have entered the viable but non-cultivable (VBNC) state. This work clearly demonstrates that L. monocytogenes can Form Biofilms in TW and that sessile cells can remain viable and cultivable in some conditions for at least the 48 h investigated. On the other hand, VBNC adaptation suggests that the pathogen can remain undetectable using traditional culture recovery techniques, which may give a false indication of processing surface hygiene status, leading to potential cross-contamination of food products.
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"Listeria monocytogenes" Can Form Biofilms in Tap Water and Enter Into the Viable but Non-Cultivable State
Microbial Ecology, 2014Co-Authors: M S Giao, C W KeevilAbstract:Listeria monocytogenes is a foodborne pathogen that can be transmitted through contaminated raw food or by ready-to-eat products that have been in contact with contaminated surfaces. Tap water (TW) is used to wash produce, as a processed food constituent and to wash processing surfaces and floors. The main aim of this work was to investigate the Formation and survival of L. monocytogenes Biofilms on stainless steel (SS) coupons in TW at 4, 22, 30 and 37 °C. For that, coupons with biofilm were visualised in situ while other coupons were scraped to quantify total cells by SYTO 9, cultivable numbers by plating onto brain heart infusion agar and viable numbers by the direct viable count method. Results showed that L. monocytogenes can Form Biofilms on SS surfaces in TW at any temperature, including at 4 °C. The number of total cells was similar for all the conditions tested while cultivable numbers varied between the level of detection (
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Mycobacterium fortuitum and Mycobacterium chelonae biofilm Formation under high and low nutrient conditions.
Journal of Applied Microbiology, 1998Co-Authors: L. Hall-stoodley, C W Keevil, H.m. Lappin-scottAbstract:The rapidly growing mycobacteria (RGM) are broadly disbursed in the environment. They have been recovered from freshwater, seawater, wastewater and even potable water samples and are increasingly associated with non-tuberculous mycobacterial disease. There is scant evidence that non-tuberculous mycobacteria (NTM) and RGM Form Biofilms. Therefore, an experimental system was designed to assess the ability of RGM to Form Biofilms under controlled laboratory conditions. A flat plate reactor flow cell was attached to either a high or low nutrient reservoir and monitored by image analysis over time. Two surfaces were chosen for assessment ofbiofilm growth: silastic which is commonly used in medical settings and high density polyethylene (HDPE) which is prevalent in water distribution systems. The results show that Mycobacterium fortuitum and M. chelonae Formed Biofilms under both high and low nutrient conditions on both surfaces studied. These results suggest that RGM may Form Biofilms under a variety of conditions in industrial and medical environments.
Anna Skalniak - One of the best experts on this subject based on the ideXlab platform.
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the virulence of streptococcus mutans and the ability to Form Biofilms
European Journal of Clinical Microbiology & Infectious Diseases, 2014Co-Authors: Wirginia Krzyściak, Anna Jurczak, Dorota Kościelniak, Beata Bystrowska, Anna SkalniakAbstract:In some diseases, a very important role is played by the ability of bacteria to Form multi-dimensional complex structure known as biofilm. The most common disease of the oral cavity, known as dental caries, is a top leader. Streptococcus mutans, one of the many etiological factors of dental caries, is a microorganism which is able to acquire new properties allowing for the expression of pathogenicity determinants determining its virulence in specific environmental conditions. Through the mechanism of adhesion to a solid surface, S. mutans is capable of colonizing the oral cavity and also of Forming bacterial biofilm. Additional properties enabling S. mutans to colonize the oral cavity include the ability to survive in an acidic environment and specific interaction with other microorganisms colonizing this ecosystem. This review is an attempt to establish which characteristics associated with biofilm Formation—virulence determinants of S. mutans—are responsible for the development of dental caries. In order to extend the knowledge of the nature of Streptococcus infections, an attempt to face the following problems will be made: Biofilm Formation as a complex process of protein–bacterium interaction. To what extent do microorganisms of the cariogenic flora exemplified by S. mutans differ in virulence determinants “expression” from microorganisms of physiological flora? How does the environment of the oral cavity and its microorganisms affect the biofilm Formation of dominant species? How do selected inhibitors affect the biofilm Formation of cariogenic microorganisms?
Serena Ammendola - One of the best experts on this subject based on the ideXlab platform.
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Correction: Zinc is required to ensure the expression of flagella and the ability to Form Biofilms in Salmonella enterica sv Typhimurium.
Metallomics : integrated biometal science, 2016Co-Authors: Serena Ammendola, Ylenia D'amico, Barbara Chirullo, Rosanna Drumo, Domenico Ciavardelli, Paolo Pasquali, Andrea BattistoniAbstract:Correction for 'Zinc is required to ensure the expression of flagella and the ability to Form Biofilms in Salmonella enterica sv Typhimurium' by Serena Ammendola et al., Metallomics, 2016, DOI: 10.1039/c6mt00108d.
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zinc is required to ensure the expression of flagella and the ability to Form Biofilms in salmonella enterica sv typhimurium
Metallomics, 2016Co-Authors: Serena Ammendola, Barbara Chirullo, Rosanna Drumo, Paolo Pasquali, Ylenia Damico, Domenico Civardelli, Andrea BattistoniAbstract:Zinc is known to play a central role in bacterial physiology and pathogenesis. Here, we report that the accumulation of FliC, the structural subunit of Salmonella phase 1 flagella, is sharply reduced in a znuABC Salmonella enterica sv. Typhimurium strain grown in zinc-poor media. Consequently, this mutant strain lacks motility, unless it grows in zinc-replete environments. This phenotype is the consequence of a general downregulation of all the genes involved in the biosynthesis of flagella, suggesting that zinc is the cofactor of proteins involved in the initiation of the transcriptional regulatory cascade leading to flagella assembly. Competition experiments in mice demonstrated that aflagellated (fliBfljC) and znuABC strains are outcompeted by the wild type strain in the gastrointestinal tract. The fliBfljC strain overgrows a fliCfljBznuABC mutant strain, but the difference in gut colonization between these two strains is less striking than that between the wild type and the znuABC strains, suggesting that the downregulation of flagella contributes to the loss of virulence of Salmonella znuABC. The absence of either flagella or ZnuABC also impairs the ability of S. Typhimurium to produce Biofilms. Zinc suppresses this defect in the znuABC mutant but not in the aflagellated strains, highlighting the role of flagella in biofilm organization. We have also observed an increased production of the quorum sensing signal AI-2 in the znuABC strain sensing zinc deprivation, that may further contribute to the reduced ability to Form Biofilms. On the whole, our study reveals novel roles of zinc in Salmonella motility and intercellular communication.
Wirginia Krzyściak - One of the best experts on this subject based on the ideXlab platform.
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effect of histatin 5 and lysozyme on the ability of streptococcus mutans to Form Biofilms in in vitro conditions
Postȩpy higieny i medycyny doświadczalnej, 2015Co-Authors: Wirginia Krzyściak, Anna Jurczak, Jakub Piątkowski, Dorota Kościelniak, Iwona Gregorczykmaga, Iwona Kolodziej, Monika A Papiez, Dorota OlczakkowalczykAbstract:INTRODUCTION: The mechanisms of adhesion to solid surfaces enable S. mutans to colonize oral cavities and Form Biofilms, which play an important role in caries development. Additional properties enabling the survival of S. mutans in the oral cavity include its ability to survive in acidic environments and specific interactions with other microorganisms inhabiting this ecosystem. AIM OF THE STUDY: The aim of this study was to determine the antibacterial activity of saliva histatin-5 (peptide) and lysozyme (protein) against S. mutans and L. rhamnosus, as representatives of physiological flora. MATERIALS AND METHODS: The study involved strains of physiological (L. rhamnosus) and cariogenic (S. mutans) flora isolated from one patient with diagnosed early caries of the deciduous teeth. RESULTS: It was proved that the presence of probiotic L. rhamnosus bacteria in the environment had a negative impact on the ability of S. mutans to produce biofilm. Moreover, the antibacterial activity of histatin-5 was confirmed, and it inhibited S. mutans growth at concentrations of 27.2 μg/ml and 54.4 μg/ml, both individually and in a mixture with lysozyme (in a total concentration of 54.4 μg/ml). CONCLUSIONS: The data obtained constitute a promising result due to their potential future application in the prevention and early diagnosis of caries.
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the virulence of streptococcus mutans and the ability to Form Biofilms
European Journal of Clinical Microbiology & Infectious Diseases, 2014Co-Authors: Wirginia Krzyściak, Anna Jurczak, Dorota Kościelniak, Beata Bystrowska, Anna SkalniakAbstract:In some diseases, a very important role is played by the ability of bacteria to Form multi-dimensional complex structure known as biofilm. The most common disease of the oral cavity, known as dental caries, is a top leader. Streptococcus mutans, one of the many etiological factors of dental caries, is a microorganism which is able to acquire new properties allowing for the expression of pathogenicity determinants determining its virulence in specific environmental conditions. Through the mechanism of adhesion to a solid surface, S. mutans is capable of colonizing the oral cavity and also of Forming bacterial biofilm. Additional properties enabling S. mutans to colonize the oral cavity include the ability to survive in an acidic environment and specific interaction with other microorganisms colonizing this ecosystem. This review is an attempt to establish which characteristics associated with biofilm Formation—virulence determinants of S. mutans—are responsible for the development of dental caries. In order to extend the knowledge of the nature of Streptococcus infections, an attempt to face the following problems will be made: Biofilm Formation as a complex process of protein–bacterium interaction. To what extent do microorganisms of the cariogenic flora exemplified by S. mutans differ in virulence determinants “expression” from microorganisms of physiological flora? How does the environment of the oral cavity and its microorganisms affect the biofilm Formation of dominant species? How do selected inhibitors affect the biofilm Formation of cariogenic microorganisms?