The Experts below are selected from a list of 5346 Experts worldwide ranked by ideXlab platform
Jongyoon Han - One of the best experts on this subject based on the ideXlab platform.
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miniature auto perfusion bioreactor system with spiral microfluidic cell retention device
Biotechnology and Bioengineering, 2021Co-Authors: Lu Yin, Taehong Kwon, Zhangxing Lai, Menglin Shang, Majid Ebrahimi Warkiani, Roger Rosche, Chwee Teck Lim, Jongyoon HanAbstract:Medium perfusion is critical in maintaining high cell concentration in cultures. The conventional membrane filtration method for medium exchange has been challenged by the Fouling and clogging of the membrane filters in long-term cultures. In this study, we present a miniature auto-perfusion system that can be operated inside a common-size laboratory incubator. The system is equipped with a spiral microfluidic chip for cell retention to replace conventional membrane filters, which fundamentally overcomes the clogging and Fouling Problem. We showed that the system supported continuous perfusion culture of Chinese hamster ovary (CHO) cells in suspension up to 14 days without cell retention chip replacement. Compared to daily manual medium change, 25% higher CHO cell concentration can be maintained at an average auto-perfusion rate of 196 ml/day in spinner flask at 70 ml working volume (2.8 VVD). The auto-perfusion system also resulted in better cell quality at high concentrations, in terms of higher viability, more uniform and regular morphology, and fewer aggregates. We also demonstrated the potential application of the system for culturing mesenchymal stem cells on microcarriers. This miniature auto-perfusion system provides an excellent solution to maintain cell-favorable conditions and high cell concentration in small-scale cultures for research and clinical uses.
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miniature auto perfusion bioreactor system with spiral microfluidic cell retention device
Biotechnology and Bioengineering, 2021Co-Authors: Lu Yin, Taehong Kwon, Zhangxing Lai, Menglin Shang, Majid Ebrahimi Warkiani, Roger Rosche, Chwee Teck Lim, Jongyoon HanAbstract:Medium perfusion is critical in maintaining high cell concentration in cultures. The conventional membrane filtration method for medium exchange has been challenged by the Fouling and clogging of the membrane filters in long-term cultures. In this study, we present a miniature auto-perfusion system that can be operated inside a common-size laboratory incubator. The system is equipped with a spiral microfluidic chip for cell retention to replace conventional membrane filters, which fundamentally overcomes the clogging and Fouling Problem. We showed that the system supported continuous perfusion culture of Chinese hamster ovary (CHO) cells in suspension up to 14 days without cell retention chip replacement. Compared to daily manual medium change, 25% higher CHO cell concentration can be maintained at an average auto-perfusion rate of 196 mL/day in spinner flask at 70 mL working volume (2.8 VVD). The auto-perfusion system also resulted in better cell quality at high concentrations, in terms of higher viability, more uniform and regular morphology, and fewer aggregates. We also demonstrated the potential application of the system for culturing mesenchymal stem cells (MSCs) on microcarriers. This miniature auto-perfusion system provides an excellent solution to maintain cell-favourable conditions and high cell concentration in small-scale cultures for research and clinical uses. This article is protected by copyright. All rights reserved.
Lu Yin - One of the best experts on this subject based on the ideXlab platform.
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miniature auto perfusion bioreactor system with spiral microfluidic cell retention device
Biotechnology and Bioengineering, 2021Co-Authors: Lu Yin, Taehong Kwon, Zhangxing Lai, Menglin Shang, Majid Ebrahimi Warkiani, Roger Rosche, Chwee Teck Lim, Jongyoon HanAbstract:Medium perfusion is critical in maintaining high cell concentration in cultures. The conventional membrane filtration method for medium exchange has been challenged by the Fouling and clogging of the membrane filters in long-term cultures. In this study, we present a miniature auto-perfusion system that can be operated inside a common-size laboratory incubator. The system is equipped with a spiral microfluidic chip for cell retention to replace conventional membrane filters, which fundamentally overcomes the clogging and Fouling Problem. We showed that the system supported continuous perfusion culture of Chinese hamster ovary (CHO) cells in suspension up to 14 days without cell retention chip replacement. Compared to daily manual medium change, 25% higher CHO cell concentration can be maintained at an average auto-perfusion rate of 196 ml/day in spinner flask at 70 ml working volume (2.8 VVD). The auto-perfusion system also resulted in better cell quality at high concentrations, in terms of higher viability, more uniform and regular morphology, and fewer aggregates. We also demonstrated the potential application of the system for culturing mesenchymal stem cells on microcarriers. This miniature auto-perfusion system provides an excellent solution to maintain cell-favorable conditions and high cell concentration in small-scale cultures for research and clinical uses.
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miniature auto perfusion bioreactor system with spiral microfluidic cell retention device
Biotechnology and Bioengineering, 2021Co-Authors: Lu Yin, Taehong Kwon, Zhangxing Lai, Menglin Shang, Majid Ebrahimi Warkiani, Roger Rosche, Chwee Teck Lim, Jongyoon HanAbstract:Medium perfusion is critical in maintaining high cell concentration in cultures. The conventional membrane filtration method for medium exchange has been challenged by the Fouling and clogging of the membrane filters in long-term cultures. In this study, we present a miniature auto-perfusion system that can be operated inside a common-size laboratory incubator. The system is equipped with a spiral microfluidic chip for cell retention to replace conventional membrane filters, which fundamentally overcomes the clogging and Fouling Problem. We showed that the system supported continuous perfusion culture of Chinese hamster ovary (CHO) cells in suspension up to 14 days without cell retention chip replacement. Compared to daily manual medium change, 25% higher CHO cell concentration can be maintained at an average auto-perfusion rate of 196 mL/day in spinner flask at 70 mL working volume (2.8 VVD). The auto-perfusion system also resulted in better cell quality at high concentrations, in terms of higher viability, more uniform and regular morphology, and fewer aggregates. We also demonstrated the potential application of the system for culturing mesenchymal stem cells (MSCs) on microcarriers. This miniature auto-perfusion system provides an excellent solution to maintain cell-favourable conditions and high cell concentration in small-scale cultures for research and clinical uses. This article is protected by copyright. All rights reserved.
Huachang Hong - One of the best experts on this subject based on the ideXlab platform.
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a review on anaerobic membrane bioreactors applications membrane Fouling and future perspectives
Desalination, 2013Co-Authors: Hongjun Lin, Meijia Zhang, Jianrong Chen, Huachang Hong, Wei Peng, Ye ZhangAbstract:In the last years, anaerobic membrane bioreactor (AnMBR) technology is being considered as a very appealing alternative for wastewater treatment due to the significant advantages over conventional anaerobic treatment and aerobic membrane bioreactor (MBR) technology. Many articles have touted the diverse potential applications of AnMBR in various stream treatment, and membrane Fouling issues. In current review, the fundamentals of AnMBR (including advantages and configurations, membrane materials and modules, and history development), application development in various stream treatment, and membrane Fouling researches are summarized and critically assessed. The characteristics of AnMBR and aerobic MBR for wastewater treatment are also compared. AnMBR technology appears to be suitable for treatment of various streams, especially for food industrial wastewater and municipal wastewater. AnMBR treatment usually encounters more serious membrane Fouling Problem. This, however, can be remedied through various conventional and novel membrane Fouling control or cleaning measures. Based on the review, future research perspectives relating to its application and membrane Fouling research are proposed.
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osmotic pressure effect on membrane Fouling in a submerged anaerobic membrane bioreactor and its experimental verification
Bioresource Technology, 2012Co-Authors: Jianrong Chen, Meijia Zhang, Ai-jun Wang, Hongjun Lin, Huachang HongAbstract:A laboratory-scale submerged anaerobic membrane bioreactor (SAnMBR) treating sewage was used to investigate the membrane Fouling mechanism. Characterization of cake layer formed on membrane surface showed that cake layer was hydrated, rich of extracellular polymeric substances (EPS) and negative charged with the charge density of 0.21-0.46 meq/kg MLSS. Detailed analysis revealed a new membrane Fouling mechanism, osmotic pressure during cake layer filtration process due to the interception of ions. An osmotic pressure model was then developed to elaborate the existence of osmotic pressure and to estimate the contribution of osmotic pressure to membrane Fouling. The calculated results showed that osmotic pressure accounted for the largest fraction of total operation pressure, indicating that osmotic pressure generated by the retained ions was one of the major mechanisms responsible for membrane Fouling Problem in MBRs. These findings provided a new insight into membrane Fouling in MBRs. (C) 2012 Elsevier Ltd. All rights reserved.
Yonghua Zhou - One of the best experts on this subject based on the ideXlab platform.
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one step facile synthesis of graphene oxide tio2 composite as efficient photocatalytic membrane for water treatment crossflow filtration operation and membrane Fouling analysis
Chemical Engineering and Processing, 2017Co-Authors: Chenyua Zhu, Gonggang Liu, Shichao Wei, Yonghua ZhouAbstract:Abstract Graphene oxide (GO) has been continuously demonstrated as promising membrane for water purification due to its excellent hydrophilic surface properties and special interconnected 2D nanofluidic channels for ion/molecule transport. In this work, to resolve the membrane Fouling Problem for GO membrane, GO/TiO 2 membrane with the multifunction of concurrent water filtration and photodegradation for pollutants was successfully fabricated by a one-step facile approach. Crossflow filtration was applied to evaluate the separation performance by simulating the real industrial process. The as-prepared GO/TiO 2 membrane exhibited remarkable ability on photocatalytic degradation of Methylene Blue under UV light, 92% of MB could be degraded after 110 min irradiation with 4 mg of photocatalyst. The membrane Fouling could be effectively alleviated with UV light irradiation, resulting in a high flux recoverability of 96% after 100 min. Furthermore, the mechanism of membrane Fouling processes for GO/TiO 2 membrane under crossflow filtration was analyzed based on Darcylaw model. The result showed adsorption and cake layer which accounted for 46.3% and 46.0% of membrane resistance were main factors that cause GO/TiO 2 membrane Fouling. The excellent performance of GO/TiO 2 membrane provided valuable insight for its industrial application in clean water production field.
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graphene oxide triethanolamine modified titanate nanowires as photocatalytic membrane for water treatment
Chemical Engineering Journal, 2017Co-Authors: Gonggang Liu, Kai Han, Chenyuan Zhu, Yupei Gao, Yong Liu, Yonghua ZhouAbstract:Abstract Graphene oxide (GO) membranes are a promising candidate for water treatment, such as sewage purification and desalination, due to its high mechanical strength, superior flexibility and hydrophilic property. However, one of their major application challenges is the membrane Fouling, resulting in membrane blocking and poor water flux. Here, we incorporate triethanolamine (TEOA) modified titanate nanowires (TNWs) with GO membranes to resolve the Fouling Problem by taking advantage of enhanced membrane photocatalytic properties. The results show that the composite membranes exhibited remarkable photocatalytic degradation of Congon Red dyes under UV-light during filtration separation, and membrane Fouling could be significantly alleviated after UV-light irradiation, leading to high water treatment performance for long-term cycling use. Furthermore, intercalation of TNWs between GO layers can produce a great quantity of nanochannels inside the membranes such that significantly increases the water flux. Besides, the presence of TEOA can evidently improve the aqueous stability for GO membranes, which is also an essential requirement for practical application. Our work demonstrates that endowing GO membranes with photocatalytic properties is an effective approach to resolve the Fouling Problem. And the as-prepared GO/TEOA-TNWs composite membranes are a promising material with anti-Fouling function and highly-aqueous-stability for long-term practical water treatment applications.
Hongjun Lin - One of the best experts on this subject based on the ideXlab platform.
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a review on anaerobic membrane bioreactors applications membrane Fouling and future perspectives
Desalination, 2013Co-Authors: Hongjun Lin, Meijia Zhang, Jianrong Chen, Huachang Hong, Wei Peng, Ye ZhangAbstract:In the last years, anaerobic membrane bioreactor (AnMBR) technology is being considered as a very appealing alternative for wastewater treatment due to the significant advantages over conventional anaerobic treatment and aerobic membrane bioreactor (MBR) technology. Many articles have touted the diverse potential applications of AnMBR in various stream treatment, and membrane Fouling issues. In current review, the fundamentals of AnMBR (including advantages and configurations, membrane materials and modules, and history development), application development in various stream treatment, and membrane Fouling researches are summarized and critically assessed. The characteristics of AnMBR and aerobic MBR for wastewater treatment are also compared. AnMBR technology appears to be suitable for treatment of various streams, especially for food industrial wastewater and municipal wastewater. AnMBR treatment usually encounters more serious membrane Fouling Problem. This, however, can be remedied through various conventional and novel membrane Fouling control or cleaning measures. Based on the review, future research perspectives relating to its application and membrane Fouling research are proposed.
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osmotic pressure effect on membrane Fouling in a submerged anaerobic membrane bioreactor and its experimental verification
Bioresource Technology, 2012Co-Authors: Jianrong Chen, Meijia Zhang, Ai-jun Wang, Hongjun Lin, Huachang HongAbstract:A laboratory-scale submerged anaerobic membrane bioreactor (SAnMBR) treating sewage was used to investigate the membrane Fouling mechanism. Characterization of cake layer formed on membrane surface showed that cake layer was hydrated, rich of extracellular polymeric substances (EPS) and negative charged with the charge density of 0.21-0.46 meq/kg MLSS. Detailed analysis revealed a new membrane Fouling mechanism, osmotic pressure during cake layer filtration process due to the interception of ions. An osmotic pressure model was then developed to elaborate the existence of osmotic pressure and to estimate the contribution of osmotic pressure to membrane Fouling. The calculated results showed that osmotic pressure accounted for the largest fraction of total operation pressure, indicating that osmotic pressure generated by the retained ions was one of the major mechanisms responsible for membrane Fouling Problem in MBRs. These findings provided a new insight into membrane Fouling in MBRs. (C) 2012 Elsevier Ltd. All rights reserved.