The Experts below are selected from a list of 243 Experts worldwide ranked by ideXlab platform
Lingyun Liu - One of the best experts on this subject based on the ideXlab platform.
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Ultralow Fouling polyacrylamide on gold Surfaces via Surface-initiated atom transfer radical polymerization.
Biomacromolecules, 2012Co-Authors: Qingsheng Liu, Anuradha Singh, Reza Lalani, Lingyun LiuAbstract:In this work, polyacrylamide is investigated as an ultralow Fouling Surface coating to highly resist protein adsorption, cell adhesion, and bacterial attachment. Polyacrylamide was grafted on gold Surfaces via Surface-initiated atom transfer radical polymerization (ATRP). Protein adsorption from a wide range of biological media, including single protein solutions of fibrinogen, bovine serum albumin, and lysozyme, dilute and undiluted human blood serum, and dilute and undiluted human blood plasma, was studied by Surface plasmon resonance (SPR). Dependence of the protein resistance on polyacrylamide film thickness was examined. With the optimal film thickness, the adsorption amount of all three single proteins on polyacrylamide-grafted Surfaces was
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ultralow Fouling polyacrylamide on gold Surfaces via Surface initiated atom transfer radical polymerization
Biomacromolecules, 2012Co-Authors: Qingsheng Liu, Anuradha Singh, Reza Lalani, Lingyun LiuAbstract:In this work, polyacrylamide is investigated as an ultralow Fouling Surface coating to highly resist protein adsorption, cell adhesion, and bacterial attachment. Polyacrylamide was grafted on gold Surfaces via Surface-initiated atom transfer radical polymerization (ATRP). Protein adsorption from a wide range of biological media, including single protein solutions of fibrinogen, bovine serum albumin, and lysozyme, dilute and undiluted human blood serum, and dilute and undiluted human blood plasma, was studied by Surface plasmon resonance (SPR). Dependence of the protein resistance on polyacrylamide film thickness was examined. With the optimal film thickness, the adsorption amount of all three single proteins on polyacrylamide-grafted Surfaces was <3 pg/mm2, close to the detection limit of SPR. The average nonspecific adsorptions from 10% plasma, 10% serum, 100% plasma, and 100% serum onto the polyacrylamide-grafted Surfaces were 5, 6.5, 17, and 28 pg/mm2, respectively, comparable (if not better) than the ...
Ivana Víšová - One of the best experts on this subject based on the ideXlab platform.
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Modulation of Living Cell Behavior with Ultra-Low Fouling Polymer Brush Interfaces.
Macromolecular bioscience, 2020Co-Authors: Ivana Víšová, Barbora Smolková, Mariia Uzhytchak, Markéta Vrabcová, Yulia Zhigunova, Milan Houska, František Surman, Andres De Los Santos Pereira, Oleg Lunov, Alexandr DejnekaAbstract:Ultra-low Fouling and functionalizable coatings represent emerging Surface platforms for various analytical and biomedical applications such as those involving examination of cellular interactions in their native environments. Ultra-low Fouling Surface platforms as advanced interfaces enabling modulation of behavior of living cells via tuning Surface physicochemical properties are presented and studied. The state-of-art ultra-low Fouling Surface-grafted polymer brushes of zwitterionic poly(carboxybetaine acrylamide), nonionic poly(N-(2-hydroxypropyl)methacrylamide), and random copolymers of carboxybetaine methacrylamide (CBMAA) and HPMAA [p(CBMAA-co-HPMAA)] with tunable molar contents of CBMAA and HPMAA are employed. Using a model Huh7 cell line, a systematic study of Surface wettability, swelling, and charge effects on the cell growth, shape, and cytoskeleton distribution is performed. This study reveals that ultra-low Fouling interfaces with a high content of zwitterionic moieties (>65 mol%) modulate cell behavior in a distinctly different way compared to coatings with a high content of nonionic HPMAA. These differences are attributed mostly to the Surface hydration capabilities. The results demonstrate a high potential of carboxybetaine-rich ultra-low Fouling Surfaces with high hydration capabilities and minimum background signal interferences to create next-generation bioresponsive interfaces for advanced studies of living objects.
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Low-Fouling Surface plasmon resonance biosensor for multi-step detection of foodborne bacterial pathogens in complex food samples.
Biosensors & bioelectronics, 2016Co-Authors: Hana Vaisocherová-lísalová, Ivana Víšová, Tomáš Špringer, Maria Laura Ermini, Xue Chadtová Song, Jan Mrázek, Josefína Lamačová, N. Scott Lynn, Petr Šedivák, Jiří HomolaAbstract:Abstract Recent outbreaks of foodborne illnesses have shown that foodborne bacterial pathogens present a significant threat to public health, resulting in an increased need for technologies capable of fast and reliable screening of food commodities. The optimal method of pathogen detection in foods should: (i) be rapid, specific, and sensitive; (ii) require minimum sample preparation; and (iii) be robust and cost-effective, thus enabling use in the field. Here we report the use of a SPR biosensor based on ultra-low Fouling and functionalizable poly(carboxybetaine acrylamide) (pCBAA) brushes for the rapid and sensitive detection of bacterial pathogens in crude food samples utilizing a three-step detection assay. We studied both the Surface resistance to Fouling and the functional capabilities of these brushes with respect to each step of the assay, namely: (I) incubation of the sensor with crude food samples, resulting in the capture of bacteria by antibodies immobilized to the pCBAA coating, (II) binding of secondary biotinylated antibody (Ab2) to previously captured bacteria, and (III) binding of streptavidin-coated gold nanoparticles to the biotinylated Ab2 in order to enhance the sensor response. We also investigated the effects of the brush thickness on the biorecognition capabilities of the gold-grafted functionalized pCBAA coatings. We demonstrate that pCBAA-compared to standard low-Fouling OEG-based alkanethiolate self-assemabled monolayers-exhibits superior Surface resistance regarding both Fouling from complex food samples as well as the non-specific binding of S-AuNPs. We further demonstrate that a SPR biosensor based on a pCBAA brush with a thickness as low as 20 nm was capable of detecting E. coli O157:H7 and Salmonella sp. in complex hamburger and cucumber samples with extraordinary sensitivity and specificity. The limits of detection for the two bacteria in cucumber and hamburger extracts were determined to be 57 CFU/mL and 17 CFU/mL for E. coli and 7.4×103 CFU/mL and 11.7×103 CFU/mL for Salmonella sp., respectively. In addition, we demonstrate the simultaneous detection of E. coli and Salmonella sp. in hamburger sample using a multichannel SPR biosensor having appropriate functional coatings.
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rapid and sensitive detection of multiple micrornas in cell lysate by low Fouling Surface plasmon resonance biosensor
Biosensors and Bioelectronics, 2015Co-Authors: Hana Vaisocherová, Hana Šípová, Ivana Víšová, Markéta Bocková, Tomáš Špringer, Maria Laura Ermini, Xue Song, Zdeněk Krejčík, Leona Chrastinova, Ondřej PastvaAbstract:We report an ultra-low Fouling Surface plasmon resonance imaging (SPRi) biosensor for the rapid simultaneous detection of multiple miRNAs in erythrocyte lysate (EL) at subpicomolar levels without need of RNA extraction. The SPRi chips were coated with ultra-low Fouling functionalizable poly(carboxybetaine acrylamide) (pCBAA) brushes having optimized thicknesses and directly functionalized with amino-modified oligonucleotide probes. We have characterized the effect of the brush thickness on the probe loading capacity: a loading capacity of ~9.8×10(12) probes/cm(2) was achieved for pCBAA having a thickness of ~40 nm. The probe-functionalized sensor also exhibited a high resistance to Fouling from ~90% EL samples (<2 ng/cm(2)). A two-step detection assay was employed for multiplexed miRNA detection in EL. Specifically, the assay consisted of (i) a sandwich-type hybridization of the probe-functionalized pCBAA with target miRNA in EL (bound to biotinylated oligonucleotides) and (ii) the capture of streptavidin-functionalized gold nanoparticles to the aforementioned biotinylated probes. We have demonstrated that this approach enables the detection of miRNAs in EL at concentrations as low as 0.5 pM. Finally, we have confirmed the detection of four endogenous miRNAs representing a set of potential miRNA biomarkers of myelodysplastic syndrome (MDS) in clinical EL samples (miR-16, miR-181, miR-34a, and miR-125b). The results revealed significantly higher levels of miR-16 in all the clinical EL samples compared to the other measured miRNAs.
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Rapid and sensitive detection of multiple microRNAs in cell lysate by low-Fouling Surface plasmon resonance biosensor
Biosensors & bioelectronics, 2015Co-Authors: Hana Vaisocherová, Hana Šípová, Ivana Víšová, Markéta Bocková, Tomáš Špringer, Maria Laura Ermini, Xue Song, Zdeněk Krejčík, Leona Chrastinova, Ondřej PastvaAbstract:We report an ultra-low Fouling Surface plasmon resonance imaging (SPRi) biosensor for the rapid simultaneous detection of multiple miRNAs in erythrocyte lysate (EL) at subpicomolar levels without need of RNA extraction. The SPRi chips were coated with ultra-low Fouling functionalizable poly(carboxybetaine acrylamide) (pCBAA) brushes having optimized thicknesses and directly functionalized with amino-modified oligonucleotide probes. We have characterized the effect of the brush thickness on the probe loading capacity: a loading capacity of ~9.8×10(12) probes/cm(2) was achieved for pCBAA having a thickness of ~40 nm. The probe-functionalized sensor also exhibited a high resistance to Fouling from ~90% EL samples (
Qingsheng Liu - One of the best experts on this subject based on the ideXlab platform.
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Ultralow Fouling polyacrylamide on gold Surfaces via Surface-initiated atom transfer radical polymerization.
Biomacromolecules, 2012Co-Authors: Qingsheng Liu, Anuradha Singh, Reza Lalani, Lingyun LiuAbstract:In this work, polyacrylamide is investigated as an ultralow Fouling Surface coating to highly resist protein adsorption, cell adhesion, and bacterial attachment. Polyacrylamide was grafted on gold Surfaces via Surface-initiated atom transfer radical polymerization (ATRP). Protein adsorption from a wide range of biological media, including single protein solutions of fibrinogen, bovine serum albumin, and lysozyme, dilute and undiluted human blood serum, and dilute and undiluted human blood plasma, was studied by Surface plasmon resonance (SPR). Dependence of the protein resistance on polyacrylamide film thickness was examined. With the optimal film thickness, the adsorption amount of all three single proteins on polyacrylamide-grafted Surfaces was
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ultralow Fouling polyacrylamide on gold Surfaces via Surface initiated atom transfer radical polymerization
Biomacromolecules, 2012Co-Authors: Qingsheng Liu, Anuradha Singh, Reza Lalani, Lingyun LiuAbstract:In this work, polyacrylamide is investigated as an ultralow Fouling Surface coating to highly resist protein adsorption, cell adhesion, and bacterial attachment. Polyacrylamide was grafted on gold Surfaces via Surface-initiated atom transfer radical polymerization (ATRP). Protein adsorption from a wide range of biological media, including single protein solutions of fibrinogen, bovine serum albumin, and lysozyme, dilute and undiluted human blood serum, and dilute and undiluted human blood plasma, was studied by Surface plasmon resonance (SPR). Dependence of the protein resistance on polyacrylamide film thickness was examined. With the optimal film thickness, the adsorption amount of all three single proteins on polyacrylamide-grafted Surfaces was <3 pg/mm2, close to the detection limit of SPR. The average nonspecific adsorptions from 10% plasma, 10% serum, 100% plasma, and 100% serum onto the polyacrylamide-grafted Surfaces were 5, 6.5, 17, and 28 pg/mm2, respectively, comparable (if not better) than the ...
Ondřej Pastva - One of the best experts on this subject based on the ideXlab platform.
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rapid and sensitive detection of multiple micrornas in cell lysate by low Fouling Surface plasmon resonance biosensor
Biosensors and Bioelectronics, 2015Co-Authors: Hana Vaisocherová, Hana Šípová, Ivana Víšová, Markéta Bocková, Tomáš Špringer, Maria Laura Ermini, Xue Song, Zdeněk Krejčík, Leona Chrastinova, Ondřej PastvaAbstract:We report an ultra-low Fouling Surface plasmon resonance imaging (SPRi) biosensor for the rapid simultaneous detection of multiple miRNAs in erythrocyte lysate (EL) at subpicomolar levels without need of RNA extraction. The SPRi chips were coated with ultra-low Fouling functionalizable poly(carboxybetaine acrylamide) (pCBAA) brushes having optimized thicknesses and directly functionalized with amino-modified oligonucleotide probes. We have characterized the effect of the brush thickness on the probe loading capacity: a loading capacity of ~9.8×10(12) probes/cm(2) was achieved for pCBAA having a thickness of ~40 nm. The probe-functionalized sensor also exhibited a high resistance to Fouling from ~90% EL samples (<2 ng/cm(2)). A two-step detection assay was employed for multiplexed miRNA detection in EL. Specifically, the assay consisted of (i) a sandwich-type hybridization of the probe-functionalized pCBAA with target miRNA in EL (bound to biotinylated oligonucleotides) and (ii) the capture of streptavidin-functionalized gold nanoparticles to the aforementioned biotinylated probes. We have demonstrated that this approach enables the detection of miRNAs in EL at concentrations as low as 0.5 pM. Finally, we have confirmed the detection of four endogenous miRNAs representing a set of potential miRNA biomarkers of myelodysplastic syndrome (MDS) in clinical EL samples (miR-16, miR-181, miR-34a, and miR-125b). The results revealed significantly higher levels of miR-16 in all the clinical EL samples compared to the other measured miRNAs.
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Rapid and sensitive detection of multiple microRNAs in cell lysate by low-Fouling Surface plasmon resonance biosensor
Biosensors & bioelectronics, 2015Co-Authors: Hana Vaisocherová, Hana Šípová, Ivana Víšová, Markéta Bocková, Tomáš Špringer, Maria Laura Ermini, Xue Song, Zdeněk Krejčík, Leona Chrastinova, Ondřej PastvaAbstract:We report an ultra-low Fouling Surface plasmon resonance imaging (SPRi) biosensor for the rapid simultaneous detection of multiple miRNAs in erythrocyte lysate (EL) at subpicomolar levels without need of RNA extraction. The SPRi chips were coated with ultra-low Fouling functionalizable poly(carboxybetaine acrylamide) (pCBAA) brushes having optimized thicknesses and directly functionalized with amino-modified oligonucleotide probes. We have characterized the effect of the brush thickness on the probe loading capacity: a loading capacity of ~9.8×10(12) probes/cm(2) was achieved for pCBAA having a thickness of ~40 nm. The probe-functionalized sensor also exhibited a high resistance to Fouling from ~90% EL samples (
Olga Santos - One of the best experts on this subject based on the ideXlab platform.
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modified stainless steel Surfaces targeted to reduce Fouling Surface characterization
Journal of Food Engineering, 2004Co-Authors: Olga Santos, Tommy Nylander, R. Rosmaninho, G. Rizzo, S.g. Yiantsios, N. Andritsos, Anastasios J. Karabelas, Luís F. Melo, Hans Mullersteinhagen, Laurence BoulangepetermannAbstract:The Surface properties of several modified stainless steel samples were characterized according to their chemical composition, roughness, topography and wettability. The modifications tested were SiF3+ and MoS22+ ion implantation; diamond-like carbon (DLC) sputtering: DLC. DLC-Si-O and SiOx, plasma enhanced chemical vapor deposition (PECVD); autocatalytic Ni-P-PTFE and silica coating. X-ray photoelectron spectroscopy (XPS) and X-ray microanalysis were applied to determine the Surface chemical composition. Atomic force microscopy (AFM) and stylus-type instruments were used for roughness determination, and the Surface topography was imaged with AFM and scanning electron microscopy (SEM). The contact angle and Surface tension were measured with the Wilhelmy plate method and the sessile drop method. For thick modified layers, only the elements of the coating were detected at the Surface, whereas for thin layers the Surface composition determined was that of the stainless steel substrate. The roughness of the 2R (cold rolled and annealed in a protective atmosphere) Surfaces was not altered by the modification techniques (except for the Ni-P-PTFE coating), while for the 2B (cold rolled. heat treated, pickled and skinpassed) Surfaces an increase in roughness was observed. The silica coating produced Surfaces with consistent roughness, independent of which steel substrate was used. DLC sputtering and Ni-P-PTFE coating produced Surfaces with the highest roughness. All modified Surfaces revealed a similar Surface topography with the exception of the Ni-P-PTFE coating, for which the coating masked the underlying steel topography. In terms of wettability, the SiOx-plasmaCVD and NiP-PTFE coating, techniques produced the most hydrophilic and hydrophobic Surfaces, respectively. (C) 2003 Elsevier Ltd. All rights reserved. (Less)
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Modified stainless steel Surfaces targeted to reduce Fouling––Surface characterization
Journal of Food Engineering, 2004Co-Authors: Olga Santos, Tommy Nylander, R. Rosmaninho, G. Rizzo, S.g. Yiantsios, N. Andritsos, Anastasios J. Karabelas, Hans Müller-steinhagen, Luís F. Melo, Laurence Boulangé-petermannAbstract:The Surface properties of several modified stainless steel samples were characterized according to their chemical composition, roughness, topography and wettability. The modifications tested were SiF3+ and MoS22+ ion implantation; diamond-like carbon (DLC) sputtering: DLC. DLC-Si-O and SiOx, plasma enhanced chemical vapor deposition (PECVD); autocatalytic Ni-P-PTFE and silica coating. X-ray photoelectron spectroscopy (XPS) and X-ray microanalysis were applied to determine the Surface chemical composition. Atomic force microscopy (AFM) and stylus-type instruments were used for roughness determination, and the Surface topography was imaged with AFM and scanning electron microscopy (SEM). The contact angle and Surface tension were measured with the Wilhelmy plate method and the sessile drop method. For thick modified layers, only the elements of the coating were detected at the Surface, whereas for thin layers the Surface composition determined was that of the stainless steel substrate. The roughness of the 2R (cold rolled and annealed in a protective atmosphere) Surfaces was not altered by the modification techniques (except for the Ni-P-PTFE coating), while for the 2B (cold rolled. heat treated, pickled and skinpassed) Surfaces an increase in roughness was observed. The silica coating produced Surfaces with consistent roughness, independent of which steel substrate was used. DLC sputtering and Ni-P-PTFE coating produced Surfaces with the highest roughness. All modified Surfaces revealed a similar Surface topography with the exception of the Ni-P-PTFE coating, for which the coating masked the underlying steel topography. In terms of wettability, the SiOx-plasmaCVD and NiP-PTFE coating, techniques produced the most hydrophilic and hydrophobic Surfaces, respectively. (C) 2003 Elsevier Ltd. All rights reserved. (Less)