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Kazuo Miyashita - One of the best experts on this subject based on the ideXlab platform.
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analysis of Fucoxanthin content and purification of all trans Fucoxanthin from turbinaria turbinata and sargassum plagyophyllum by sio2 open column chromatography and reversed phase hplc
Journal of Liquid Chromatography & Related Technologies, 2012Co-Authors: Irwandi Jaswi, Dedi Noviendri, Hamzah Mohd Salleh, Muhammad Tahe, Kazuo Miyashita, Nazaruddi RamliAbstract:Reversed phase-high performance liquid chromatography (RP-HPLC) was used to analyze Fucoxanthin content of two species of Malaysian brown seaweeds, Turbinaria turbinate, and Sargassum plagyophyllum. The Fucoxanthin contents of T. turbinata and S. plagyophyllum were 0.59 ± 0.08 and 0.71 ± 0.01 mg/g dry-weight, respectively. Ultraviolet spectrum of Fucoxanthin showed the maximum absorbance at 450 nm. Furthermore, the HPLC chromatograms of purified all-trans-Fucoxanthin from showed one major peak with a retention time of 7.8 min.
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inhibition of proliferation of a hepatoma cell line by Fucoxanthin in relation to cell cycle arrest and enhanced gap junctional intercellular communication
Chemico-Biological Interactions, 2009Co-Authors: Yungsheng Huang, Kazuo Miyashita, Masashi Hosokawa, Miaolin HuAbstract:Abstract Fucoxanthin is one of the most abundant carotenoids found in Undaria pinnatifida and has been shown to inhibit tumor proliferation in vitro. However, the mechanisms underlying the anti-cancer effects of Fucoxanthin are unclear. In this study, we hypothesized that Fucoxanthin may cause cell cycle arrest and enhance gap junctional intercellular communication (GJIC) in SK-Hep-1 human hepatoma cells. Data revealed that Fucoxanthin (1–20 μM) strongly and concentration-dependently inhibited the proliferation of SK-Hep-1 cells at 24 h of incubation, whereas Fucoxanthin facilitated the growth of a murine embryonic hepatic (BNL CL.2) cells at 24 h of incubation and only slightly slowed the cell proliferation at 48 h. In SK-Hep-1 cells, Fucoxanthin caused cell cycle arrest at G0/G1 phase and induced cell apoptosis, as evidenced by increased subG1 cells and induction of DNA strand breaks. Using scrape loading-dye-transfer assay, Fucoxanthin was found to significantly enhance GJIC of SK-Hep-1 cells without affecting that of BNL CL.2 cells. In addition, Fucoxanthin significantly increased protein and mRNA expressions of connexin 43 (Cx43) and connexin 32 (Cx32) in SK-Hep-1 cells. Moreover, Fucoxanthin markedly increased the concentration of intracellular calcium levels in SK-Hep-1 cells. Thus, Fucoxanthin is specifically antiproliferative against SK-Hep-1 cells, and the effect is associated with upregulation of Cx32 and Cx43, which enhances GJIC of SK-Hep-1 cells. The enhanced GJIC may be responsible for the increase of the intracellular calcium level, which then causes cell cycle arrest and apoptosis.
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Seaweed carotenoid, Fucoxanthin, as a multi-functional nutrient.
Asia Pacific Journal of Clinical Nutrition, 2008Co-Authors: Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Takayuki Tsukui, Kazuo MiyashitaAbstract:Fucoxanthin has a unique structure including an unusual allenic bond and 5, 6-monoepoxide in its molecule. We found that abdominal white adipose tissue (WAT) weights of rats and mice fed Fucoxanthin were significantly lower than those fed a control diet. The daily intake of Fucoxanthin in mice also caused a significant reductions of body weight. Clear signals of uncoupling protein 1 (UCP1) and its mRNA were detected by Western and Northern blot analyses in abdominal WAT in mice fed Fucoxanthin, although there is little expression of UCP1 in WAT in mice fed a control diet. UCP1 expression in WAT by Fucoxanthin intake leads to oxidation of fatty acids and heat production in WAT mitochondria. Substrate oxidation can directly reduce WAT in animals. Fucoxanthin intake also significantly reduced blood glucose and plasma insulin. Furthermore, feeding Fucoxanthin significantly increased the level of hepatic docosahexaenoic acid (DHA), a most important n-3 functional polyunsaturated fatty acid in biological systems. These multi-functionalities of Fucoxanthin indicate that it is an important bioactive carotenoid that is beneficial for the prevention of the metabolicsyndrome.
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dietary combination of Fucoxanthin and fish oil attenuates the weight gain of white adipose tissue and decreases blood glucose in obese diabetic kk ay mice
Journal of Agricultural and Food Chemistry, 2007Co-Authors: Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Kazuo MiyashitaAbstract:Fucoxanthin is a marine carotenoid found in edible brown seaweeds. We previously reported that dietary Fucoxanthin attenuates the weight gain of white adipose tissue (WAT) of diabetic/obese KK- A(y) mice. In this study, to evaluate the antiobesity and antidiabetic effects of Fucoxanthin and fish oil, we investigated the effect on the WAT weight, blood glucose, and insulin levels of KK- A(y) mice. Furthermore, the expression level of uncoupling protein 1 (UCP1) and adipokine mRNA in WAT were measured. After 4 weeks of feeding, 0.2% Fucoxanthin in the diet markedly attenuated the gain of WAT weight in KK- A(y) mice with increasing UCP1 expression compared with the control mice. The WAT weight of the mice fed 0.1% Fucoxanthin and 6.9% fish oil was also significantly lower than that of the mice fed Fucoxanthin alone. In addition, 0.2% Fucoxanthin markedly decreased the blood glucose and plasma insulin concentrations in KK- A(y) mice. The mice fed with the combination diet of 0.1% Fucoxanthin and fish oil also showed improvements similar to that of 0.2% Fucoxanthin. Leptin and tumor necrosis factor (TNFalpha) mRNA expression in WAT were significantly down-regulated by 0.2% Fucoxanthin. These results suggest that dietary Fucoxanthin decreases the blood glucose and plasma insulin concentration of KK- A(y) along with down-regulating TNFalpha mRNA. In addition, the combination of Fucoxanthin and fish oil is more effective for attenuating the weight gain of WAT than feeding with Fucoxanthin alone.
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dietary combination of Fucoxanthin and fish oil attenuates the weight gain of white adipose tissue and decreases blood glucose in obese diabetic kk ay mice
Journal of Agricultural and Food Chemistry, 2007Co-Authors: Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Kazuo MiyashitaAbstract:Fucoxanthin is a marine carotenoid found in edible brown seaweeds. We previously reported that dietary Fucoxanthin attenuates the weight gain of white adipose tissue (WAT) of diabetic/obese KK-Ay mice. In this study, to evaluate the antiobesity and antidiabetic effects of Fucoxanthin and fish oil, we investigated the effect on the WAT weight, blood glucose, and insulin levels of KK-Ay mice. Furthermore, the expression level of uncoupling protein 1 (UCP1) and adipokine mRNA in WAT were measured. After 4 weeks of feeding, 0.2% Fucoxanthin in the diet markedly attenuated the gain of WAT weight in KK-Ay mice with increasing UCP1 expression compared with the control mice. The WAT weight of the mice fed 0.1% Fucoxanthin and 6.9% fish oil was also significantly lower than that of the mice fed Fucoxanthin alone. In addition, 0.2% Fucoxanthin markedly decreased the blood glucose and plasma insulin concentrations in KK-Ay mice. The mice fed with the combination diet of 0.1% Fucoxanthin and fish oil also showed impr...
Masashi Hosokawa - One of the best experts on this subject based on the ideXlab platform.
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biosynthetic pathway and health benefits of Fucoxanthin an algae specific xanthophyll in brown seaweeds
International Journal of Molecular Sciences, 2013Co-Authors: Koji Mikami, Masashi HosokawaAbstract:Fucoxanthin is the main carotenoid produced in brown algae as a component of the light-harvesting complex for photosynthesis and photoprotection. In contrast to the complete elucidation of the carotenoid biosynthetic pathways in red and green algae, the biosynthetic pathway of Fucoxanthin in brown algae is not fully understood. Recently, two models for the Fucoxanthin biosynthetic pathway have been proposed in unicellular diatoms; however, there is no such information for the pathway in brown seaweeds to date. Here, we propose a biosynthetic pathway for Fucoxanthin in the brown seaweed, Ectocarpus siliculosus, derived from comparison of carotenogenic genes in its sequenced genome with those in the genomes of two diatoms, Thalassiosira pseudonana and Phaeodactylum tricornutum. Currently, Fucoxanthin is receiving attention, due to its potential benefits for human health. Therefore, new knowledge regarding the medical and nutraceutical properties of Fucoxanthin from brown seaweeds is also summarized here.
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inhibition of proliferation of a hepatoma cell line by Fucoxanthin in relation to cell cycle arrest and enhanced gap junctional intercellular communication
Chemico-Biological Interactions, 2009Co-Authors: Yungsheng Huang, Kazuo Miyashita, Masashi Hosokawa, Miaolin HuAbstract:Abstract Fucoxanthin is one of the most abundant carotenoids found in Undaria pinnatifida and has been shown to inhibit tumor proliferation in vitro. However, the mechanisms underlying the anti-cancer effects of Fucoxanthin are unclear. In this study, we hypothesized that Fucoxanthin may cause cell cycle arrest and enhance gap junctional intercellular communication (GJIC) in SK-Hep-1 human hepatoma cells. Data revealed that Fucoxanthin (1–20 μM) strongly and concentration-dependently inhibited the proliferation of SK-Hep-1 cells at 24 h of incubation, whereas Fucoxanthin facilitated the growth of a murine embryonic hepatic (BNL CL.2) cells at 24 h of incubation and only slightly slowed the cell proliferation at 48 h. In SK-Hep-1 cells, Fucoxanthin caused cell cycle arrest at G0/G1 phase and induced cell apoptosis, as evidenced by increased subG1 cells and induction of DNA strand breaks. Using scrape loading-dye-transfer assay, Fucoxanthin was found to significantly enhance GJIC of SK-Hep-1 cells without affecting that of BNL CL.2 cells. In addition, Fucoxanthin significantly increased protein and mRNA expressions of connexin 43 (Cx43) and connexin 32 (Cx32) in SK-Hep-1 cells. Moreover, Fucoxanthin markedly increased the concentration of intracellular calcium levels in SK-Hep-1 cells. Thus, Fucoxanthin is specifically antiproliferative against SK-Hep-1 cells, and the effect is associated with upregulation of Cx32 and Cx43, which enhances GJIC of SK-Hep-1 cells. The enhanced GJIC may be responsible for the increase of the intracellular calcium level, which then causes cell cycle arrest and apoptosis.
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Seaweed carotenoid, Fucoxanthin, as a multi-functional nutrient.
Asia Pacific Journal of Clinical Nutrition, 2008Co-Authors: Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Takayuki Tsukui, Kazuo MiyashitaAbstract:Fucoxanthin has a unique structure including an unusual allenic bond and 5, 6-monoepoxide in its molecule. We found that abdominal white adipose tissue (WAT) weights of rats and mice fed Fucoxanthin were significantly lower than those fed a control diet. The daily intake of Fucoxanthin in mice also caused a significant reductions of body weight. Clear signals of uncoupling protein 1 (UCP1) and its mRNA were detected by Western and Northern blot analyses in abdominal WAT in mice fed Fucoxanthin, although there is little expression of UCP1 in WAT in mice fed a control diet. UCP1 expression in WAT by Fucoxanthin intake leads to oxidation of fatty acids and heat production in WAT mitochondria. Substrate oxidation can directly reduce WAT in animals. Fucoxanthin intake also significantly reduced blood glucose and plasma insulin. Furthermore, feeding Fucoxanthin significantly increased the level of hepatic docosahexaenoic acid (DHA), a most important n-3 functional polyunsaturated fatty acid in biological systems. These multi-functionalities of Fucoxanthin indicate that it is an important bioactive carotenoid that is beneficial for the prevention of the metabolicsyndrome.
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dietary combination of Fucoxanthin and fish oil attenuates the weight gain of white adipose tissue and decreases blood glucose in obese diabetic kk ay mice
Journal of Agricultural and Food Chemistry, 2007Co-Authors: Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Kazuo MiyashitaAbstract:Fucoxanthin is a marine carotenoid found in edible brown seaweeds. We previously reported that dietary Fucoxanthin attenuates the weight gain of white adipose tissue (WAT) of diabetic/obese KK- A(y) mice. In this study, to evaluate the antiobesity and antidiabetic effects of Fucoxanthin and fish oil, we investigated the effect on the WAT weight, blood glucose, and insulin levels of KK- A(y) mice. Furthermore, the expression level of uncoupling protein 1 (UCP1) and adipokine mRNA in WAT were measured. After 4 weeks of feeding, 0.2% Fucoxanthin in the diet markedly attenuated the gain of WAT weight in KK- A(y) mice with increasing UCP1 expression compared with the control mice. The WAT weight of the mice fed 0.1% Fucoxanthin and 6.9% fish oil was also significantly lower than that of the mice fed Fucoxanthin alone. In addition, 0.2% Fucoxanthin markedly decreased the blood glucose and plasma insulin concentrations in KK- A(y) mice. The mice fed with the combination diet of 0.1% Fucoxanthin and fish oil also showed improvements similar to that of 0.2% Fucoxanthin. Leptin and tumor necrosis factor (TNFalpha) mRNA expression in WAT were significantly down-regulated by 0.2% Fucoxanthin. These results suggest that dietary Fucoxanthin decreases the blood glucose and plasma insulin concentration of KK- A(y) along with down-regulating TNFalpha mRNA. In addition, the combination of Fucoxanthin and fish oil is more effective for attenuating the weight gain of WAT than feeding with Fucoxanthin alone.
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dietary combination of Fucoxanthin and fish oil attenuates the weight gain of white adipose tissue and decreases blood glucose in obese diabetic kk ay mice
Journal of Agricultural and Food Chemistry, 2007Co-Authors: Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Kazuo MiyashitaAbstract:Fucoxanthin is a marine carotenoid found in edible brown seaweeds. We previously reported that dietary Fucoxanthin attenuates the weight gain of white adipose tissue (WAT) of diabetic/obese KK-Ay mice. In this study, to evaluate the antiobesity and antidiabetic effects of Fucoxanthin and fish oil, we investigated the effect on the WAT weight, blood glucose, and insulin levels of KK-Ay mice. Furthermore, the expression level of uncoupling protein 1 (UCP1) and adipokine mRNA in WAT were measured. After 4 weeks of feeding, 0.2% Fucoxanthin in the diet markedly attenuated the gain of WAT weight in KK-Ay mice with increasing UCP1 expression compared with the control mice. The WAT weight of the mice fed 0.1% Fucoxanthin and 6.9% fish oil was also significantly lower than that of the mice fed Fucoxanthin alone. In addition, 0.2% Fucoxanthin markedly decreased the blood glucose and plasma insulin concentrations in KK-Ay mice. The mice fed with the combination diet of 0.1% Fucoxanthin and fish oil also showed impr...
Hayato Maeda - One of the best experts on this subject based on the ideXlab platform.
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Seaweed carotenoid, Fucoxanthin, as a multi-functional nutrient.
Asia Pacific Journal of Clinical Nutrition, 2008Co-Authors: Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Takayuki Tsukui, Kazuo MiyashitaAbstract:Fucoxanthin has a unique structure including an unusual allenic bond and 5, 6-monoepoxide in its molecule. We found that abdominal white adipose tissue (WAT) weights of rats and mice fed Fucoxanthin were significantly lower than those fed a control diet. The daily intake of Fucoxanthin in mice also caused a significant reductions of body weight. Clear signals of uncoupling protein 1 (UCP1) and its mRNA were detected by Western and Northern blot analyses in abdominal WAT in mice fed Fucoxanthin, although there is little expression of UCP1 in WAT in mice fed a control diet. UCP1 expression in WAT by Fucoxanthin intake leads to oxidation of fatty acids and heat production in WAT mitochondria. Substrate oxidation can directly reduce WAT in animals. Fucoxanthin intake also significantly reduced blood glucose and plasma insulin. Furthermore, feeding Fucoxanthin significantly increased the level of hepatic docosahexaenoic acid (DHA), a most important n-3 functional polyunsaturated fatty acid in biological systems. These multi-functionalities of Fucoxanthin indicate that it is an important bioactive carotenoid that is beneficial for the prevention of the metabolicsyndrome.
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dietary combination of Fucoxanthin and fish oil attenuates the weight gain of white adipose tissue and decreases blood glucose in obese diabetic kk ay mice
Journal of Agricultural and Food Chemistry, 2007Co-Authors: Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Kazuo MiyashitaAbstract:Fucoxanthin is a marine carotenoid found in edible brown seaweeds. We previously reported that dietary Fucoxanthin attenuates the weight gain of white adipose tissue (WAT) of diabetic/obese KK- A(y) mice. In this study, to evaluate the antiobesity and antidiabetic effects of Fucoxanthin and fish oil, we investigated the effect on the WAT weight, blood glucose, and insulin levels of KK- A(y) mice. Furthermore, the expression level of uncoupling protein 1 (UCP1) and adipokine mRNA in WAT were measured. After 4 weeks of feeding, 0.2% Fucoxanthin in the diet markedly attenuated the gain of WAT weight in KK- A(y) mice with increasing UCP1 expression compared with the control mice. The WAT weight of the mice fed 0.1% Fucoxanthin and 6.9% fish oil was also significantly lower than that of the mice fed Fucoxanthin alone. In addition, 0.2% Fucoxanthin markedly decreased the blood glucose and plasma insulin concentrations in KK- A(y) mice. The mice fed with the combination diet of 0.1% Fucoxanthin and fish oil also showed improvements similar to that of 0.2% Fucoxanthin. Leptin and tumor necrosis factor (TNFalpha) mRNA expression in WAT were significantly down-regulated by 0.2% Fucoxanthin. These results suggest that dietary Fucoxanthin decreases the blood glucose and plasma insulin concentration of KK- A(y) along with down-regulating TNFalpha mRNA. In addition, the combination of Fucoxanthin and fish oil is more effective for attenuating the weight gain of WAT than feeding with Fucoxanthin alone.
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dietary combination of Fucoxanthin and fish oil attenuates the weight gain of white adipose tissue and decreases blood glucose in obese diabetic kk ay mice
Journal of Agricultural and Food Chemistry, 2007Co-Authors: Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Kazuo MiyashitaAbstract:Fucoxanthin is a marine carotenoid found in edible brown seaweeds. We previously reported that dietary Fucoxanthin attenuates the weight gain of white adipose tissue (WAT) of diabetic/obese KK-Ay mice. In this study, to evaluate the antiobesity and antidiabetic effects of Fucoxanthin and fish oil, we investigated the effect on the WAT weight, blood glucose, and insulin levels of KK-Ay mice. Furthermore, the expression level of uncoupling protein 1 (UCP1) and adipokine mRNA in WAT were measured. After 4 weeks of feeding, 0.2% Fucoxanthin in the diet markedly attenuated the gain of WAT weight in KK-Ay mice with increasing UCP1 expression compared with the control mice. The WAT weight of the mice fed 0.1% Fucoxanthin and 6.9% fish oil was also significantly lower than that of the mice fed Fucoxanthin alone. In addition, 0.2% Fucoxanthin markedly decreased the blood glucose and plasma insulin concentrations in KK-Ay mice. The mice fed with the combination diet of 0.1% Fucoxanthin and fish oil also showed impr...
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Fucoxanthin and its metabolite Fucoxanthinol suppress adipocyte differentiation in 3t3 l1 cells
International Journal of Molecular Medicine, 2006Co-Authors: Hayato Maeda, Masashi Hosokawa, Tokutake Sashima, Nobuyuki Takahashi, Teruo Kawada, Kazuo MiyashitaAbstract:Fucoxanthin is a major carotenoid found in edible seaweed such as Undaria pinnatifida and Hijikia fusiformis. We investigated the suppressive effects of Fucoxanthin and its metabolite, Fucoxanthinol, on the differentiation of 3T3-L1 preadipocytes to adipocytes. Fucoxanthin inhibited intercellular lipid accumulation during adipocyte differentiation of 3T3-L1 cells. Furthermore, Fucoxanthin was converted to Fucoxanthinol in 3T3-L1 cells. Fucoxanthinol also exhibited suppressive effects on lipid accumulation and decreased glycerol-3-phosphate dehydrogenase activity, an indicator of adipocyte differentiation. The suppressive effect of Fucoxanthinol was stronger than that of Fucoxanthin. In addition, in 3T3-L1 cells treated with Fucoxanthin and Fucoxanthinol, peroxisome proliferator-activated receptor gamma (PPARgamma), which regulates adipogenic gene expression, was down-regulated in a dose-dependent manner. These results suggest that Fucoxanthin and Fucoxanthinol inhibit the adipocyte differentiation of 3T3-L1 cells through down-regulation of PPARgamma. Fucoxanthinol had stronger suppressive effects than Fucoxanthin on adipocyte differentiation in 3T3-L1 cells.
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Fucoxanthin induces apoptosis and enhances the antiproliferative effect of the pparγ ligand troglitazone on colon cancer cells
Biochimica et Biophysica Acta, 2004Co-Authors: Masashi Hosokawa, Hayato Maeda, Masahiro Kudo, Hiroyuki Kohno, Takuji Tanaka, Kazuo MiyashitaAbstract:The effect of Fucoxanthin, from the edible seaweed Undaria pinnatifida on viability of colon cancer cells and induction of apoptosis was investigated. Fucoxanthin remarkably reduced the viability of human colon cancer cell lines, Caco-2, HT-29 and DLD-1. Furthermore, treatment with Fucoxanthin induced DNA fragmentation, indicating apoptosis. The DNA fragmentation in Caco-2 cells treated with 22.6 microM Fucoxanthin for 24 h was 10-fold higher than in the control. Fucoxanthin suppressed the level of Bcl-2 protein. Also, DNA fragmentation induced by Fucoxanthin was partially inhibited by a caspase inhibitor Z-VAD-fmk. Moreover, combined treatment with 3.8 microM Fucoxanthin and 10 microM troglitazone, which is a specific ligand for peroxisome proliferator-activated receptor (PPAR) gamma, effectively decreased the viability of Caco-2 cells. However, separate treatments with these same concentrations of Fucoxanthin nor troglitazone did not affect cell viability. These findings indicate that Fucoxanthin may act as a chemopreventive and/or chemotherapeutic carotenoid in colon cancer cells by modulating cell viability in combination with troglitazone.
Sang Min Kim - One of the best experts on this subject based on the ideXlab platform.
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Fucoxanthin biosynthesis has a positive correlation with the specific growth rate in the culture of microalga Phaeodactylum tricornutum
Journal of Applied Phycology, 2021Co-Authors: Do Yeon Kwon, Trang Thi Vuong, Jaeyoung Choi, Taek Sung Lee, Song Yi Koo, Keum Taek Hwang, Sang Min KimAbstract:In this study, the correlation between the specific growth rate of Phaeodactylum tricornutum and Fucoxanthin (Fucoxanthin) biosynthesis was investigated both in batch and in semi-continuous cultures. Fucoxanthin content from P. tricornutum biomass showed a positive correlation with specific growth rate during the normal culture period. The maximum yield of Fucoxanthin (2.42 mg g^−1 dry biomass) was observed at day 5, and the highest specific growth rate (1.91) was observed at day 4, both of which were during the exponential phase. Upon treatment with growth inhibitors (AZD-8055 or norflurazon) during the exponential phase, Fucoxanthin content decreased with growth rate in a concentration-dependent manner. In a semi-continuous culture of P. tricornutum with dilution rates from 0.1 to 0.5 day^−1, we found a positive correlation between specific growth rate and Fucoxanthin biosynthesis in a steady-state condition. Based on the transcriptome analysis results, different metabolic and key regulatory genes were active at different growth phases. Particularly, during the exponential growth phase, various genes related to regulatory mechanisms, such as cell growth and replication, were expressed, whereas in the stationary phase, their expression was reduced. In the Fucoxanthin biosynthesis pathway, 1-deoxy- d -erythritol 2,4-cyclodiphosphate synthase ( ISPD ) and violaxanthin deepoxidase ( VDE ) genes showed higher expression levels in the exponential phase, thus indicating that they are critical genes for the regulation of Fucoxanthin biosynthesis in P. tricornutum . Our results are valuable to our understanding of the basic mechanism of Fucoxanthin biosynthesis, thus providing theoretical guidance for the commercial production of Fucoxanthin derived from P. tricornutum . Graphical abstract
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Effects of temperature, light, and pH on the stability of Fucoxanthin in an oil-in-water emulsion.
Food chemistry, 2019Co-Authors: Dong Zhao, Sang Min Kim, Cheol-ho Pan, Moojoong Kim, Gun-hee Kim, Donghwa ChungAbstract:Abstract The effects of temperature, light, and pH on the stability of Fucoxanthin in an oil-in-water emulsion were investigated with analyzing the kinetics and thermodynamics of Fucoxanthin degradation. In the absence of light and air at pH 4.6, increasing the temperature from 25 to 60 °C significantly promoted Fucoxanthin degradation. Total and all-trans Fucoxanthin demonstrated an energetically unfavorable, non-spontaneous degradation with an Arrhenius temperature dependence. Increasing the light intensity up to 2000 lx at 25 °C and pH 4.6 caused a sharp degradation of total, all-trans, 13-cis, and 13′-cis Fucoxanthin, but promoted the formation of the 9′-cis isomer. In the absence of light and air at 25 °C, decreasing the pH to 1.2 caused significant Fucoxanthin degradation, whereas increasing the pH to 7.4 retarded the degradation. The property with the greatest influence on Fucoxanthin stability was pH, followed by temperature and then light. Total and all-trans Fucoxanthin followed first-order degradation kinetics.
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Development, Quantification, Method Validation, and Stability Study of a Novel Fucoxanthin-Fortified Milk
Journal of agricultural and food chemistry, 2016Co-Authors: Il-kyoon Mok, Cheol-ho Pan, Jung-ro Yoon, Sang Min KimAbstract:To extend the scope of application of Fucoxanthin, a marine carotenoid, whole milk (WM) and skimmed milk (SM) were fortified with Fucoxanthin isolated from the microalga Phaeodactylum tricornutum to a final 8 μg/mL milk solution concentration. Using these liquid systems, a Fucoxanthin analysis method implementing extraction and HPLC-DAD was developed and validated by accuracy, precision, system suitability, and robustness tests. The current method demonstrated good linearity over the range of 0.125–100 μg/mL Fucoxanthin with R2 = 1.0000, and all validation data supported its adequacy for use in Fucoxanthin analysis from milk solution. To investigate Fucoxanthin stability during milk production and distribution, Fucoxanthin content was examined during storage, pasteurization, and drying processes under various conditions. Fucoxanthin in milk solutions showed better stabilizing effect in 1 month of storage period. Degradation rate constant (k) on Fucoxanthin during this storage period suggested that fucoxan...
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Fucoxanthin as a major carotenoid in Isochrysis aff. galbana: Characterization of extraction for commercial application
Journal of the Korean Society for Applied Biological Chemistry, 2012Co-Authors: Sang Min Kim, Suk-woo Kang, O-nam Kwon, Donghwa Chung, Cheol-ho PanAbstract:Fucoxanthin, a main marine carotenoid, in five species of Fucoxanthin-containing microalgae, was quantified by high-performance liquid chromatography. Among the studied species, Isochrysis aff. galbana contained the highest amount of Fucoxanthin (18.23 mg/g dried sample). This microalga showed good Fucoxanthin extraction efficiency under the tested solvents (methanol, ethanol, acetone, and ethyl acetate), with the exception of n-hexane. In addition, most Fucoxanthin (∼95%) could be extracted by a single extraction in ethanol within 5 min, and only 15% degradation of Fucoxanthin was detected during ethanol extraction for 24 h. The two-phase solvent system of n -hexaneethanol-water with a volume ratio of 10:9:1 was determined to be the best system for the separation of Fucoxanthin and lipids from extracts of I . aff. galbana . Under these conditions, Fucoxanthin was fractionated in the hydroalcohol phase apart from the hexane phase containing lipids. These results imply that I . aff. galbana can be a commercial source for the spontaneous production of valuable Fucoxanthins and lipids.
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a preparative method for isolation of Fucoxanthin from eisenia bicyclis by centrifugal partition chromatography
Phytochemical Analysis, 2011Co-Authors: Sang Min Kim, Ya Fang ShangAbstract:Introduction – Eisenia bicyclis (Kjellman) Setchell (Laminariaceae) is a common brown alga that inhabits around the coast of Korea, Japan and China. It contains Fucoxanthin, a major carotenoid of brown algae which shows a variety of pharmaceutical functions. Objective – The aim of this investigation was the quantification and preparative isolation of Fucoxanthin from fresh E. bicyclis using a new separation scheme, centrifugal partition chromatography (CPC). Methodology – The Fucoxanthin fraction (Fuco fraction) was prepared by solvent partition method from the acetone extract of fresh E. bicyclis. Fuco fraction was used for CPC using a two-phase solvent system of n-hexane–ethyl acetate–ethanol–water (5:5:7:3, v/v/v/v). The flow rate of mobile phase was 2 mL/min with descending mode while rotating at 1000 rpm. The eluate was monitored at 410 nm. The content and structure of Fucoxanthin in the CPC fraction were confirmed with HPLC, UV, APCI/MS and NMR spectra. Results – A preparative CPC yielded 20 mg of Fucoxanthin (87% recovery from Fuco fraction) in a two-step separation from 516 mg of Fuco fraction containing 4.59% Fucoxanthin. The purity of the isolated Fucoxanthin was about 81% in the first CPC step and over 98% in the second CPC step based on the calibration curve. The isolated Fucoxanthin was identified as all-trans-Fucoxanthin with APCI/MS (parent ion at m/z 641 [M + H − H2O]+) and 1H, 13C and 2-D NMR spectra. Conclusion – High-purity Fucoxanthin was successfully isolated from fresh E. bicyclis, suggesting further potential applications in the industrial use of this valuable carotenoid. Copyright © 2011 John Wiley & Sons, Ltd.
Soojin Heo - One of the best experts on this subject based on the ideXlab platform.
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anti inflammatory effect of Fucoxanthin derivatives isolated from sargassum siliquastrum in lipopolysaccharide stimulated raw 264 7 macrophage
Food and Chemical Toxicology, 2012Co-Authors: Soojin Heo, Weonjong Yoon, Kilnam Kim, Youngung Choi, Kontak Yoon, Dohyung Kang, Zhongji Qian, Ilwhan Choi, Wonkyo JungAbstract:In this study, the anti-inflammatory effect of Fucoxanthin (FX) derivatives, which was isolated from Sargassum siliquastrum were evaluated by examining their inhibitory effects on pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells. The FX derivatives were isolated from activity-guided chloroform fraction using inhibition of nitric oxide (NO) production and identified as 9'-cis-(6'R) fucoxnathin (FXA), and 13-cis and 13'-cis-(6'R) Fucoxanthin complex (FXB) on the basis of a comparison of NMR spectroscopic data. Both FXA and FXB significantly inhibited the NO production and showed slightly reduce the PGE2 production. However, FXB exhibited cytotoxicity at the whole tested concentration, therefore, the results of FXA was only illustrate for further experiments. FXA induced dose-dependent reduction in the inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) proteins as well as mRNA expression. In addition, FXA reduced the LPS-stimulated production and mRNA expressions of TNF-α and IL-6 in a dose-dependent manner whereas IL-1β production do not inhibit by addition of FXA. Taken together, these findings indicate that the anti-inflammatory properties of FXA may be due to the inhibition of iNOS/NO pathway which associated with the attenuation of TNF-α and IL-6 formation. Thus FXA may provide a potential therapeutic approach for inflammation related diseases.
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Fucoxanthin induces apoptosis in human leukemia hl 60 cells through a ros mediated bcl xl pathway
Toxicology in Vitro, 2010Co-Authors: Kilnam Kim, Soojin Heo, Sungmyung Kang, Ginnae Ahn, Youjin JeonAbstract:Fucoxanthin, a natural biologically active substance isolated from Ishige okamurae, evidences antitumor activity in human leukemia cell HL-60 cells via the induction of apoptosis. However, the mechanism underlying Fucoxanthin-induced apoptosis in HL-60 cells remains unclear. In this study, we focused on the effect of Fucoxanthin induction on the accumulation of reactive oxygen species (ROS), and on the triggering of Bcl-xL signaling pathway in HL-60 cells. We determined that ROS are generated during Fucoxanthin-induced cytotoxicity and apoptosis in HL-60 cells, and that N-acetylcysteine (NAC), a ROS scavenger, suppressed Fucoxanthin-induced cytotoxicity and apoptosis. Moreover, Fucoxanthin-induced the cleavage of caspases -3 and -7, and poly-ADP-ribose polymerase (PARP) and a decrease of Bcl-xL levels, whereas NAC pre-treatment significantly inhibited caspase-3, -7, and PARP cleavage and the reduction in Bcl-xL levels. In this study, it was demonstrated for the first time that Fucoxanthin generated ROS and that the accumulation of ROS performed a crucial role in the Fucoxanthin-induced Bcl-xL signaling pathway.
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protective effect of Fucoxanthin isolated from sargassum siliquastrum on uv b induced cell damage
Journal of Photochemistry and Photobiology B-biology, 2009Co-Authors: Soojin Heo, Youjin JeonAbstract:Abstract Fucoxanthin is a carotenoid isolated from Sargassum siliquastrum and is considered to be one of major active compound of marine algae. In this study, we investigated and confirmed the protective effect of Fucoxanthin on UV-B induced cell injury in human fibroblast via 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT), and comet assays. Intracellular ROS generated by exposure to UV-B radiation, which was significantly decreased by addition with various concentrations of Fucoxanthin. Cell survival rate was increased with Fucoxanthin pre-treated cells, which was reached around 81.47% at 100 μM, and the inhibitory effect of cell damage exhibited dose-dependent manner. Moreover, Fucoxanthin having protective properties was demonstrated via Hoechst 33342/PI staining. Hence, on the basis of the above-mentioned studies, Fucoxanthin has the ability to protect against oxidative stress induced by UV-B radiation and which might be applied to antioxidant and cosmeceutical industries.
