The Experts below are selected from a list of 19221 Experts worldwide ranked by ideXlab platform
Yukui Zhang - One of the best experts on this subject based on the ideXlab platform.
-
tailor made boronic acid Functionalized magnetic nanoparticles with a tunable polymer shell assisted for the selective enrichment of glycoproteins glycopeptides
ACS Applied Materials & Interfaces, 2015Co-Authors: Xihao Zhang, Xiwen He, Langxing Chen, Jiewen Wang, Yukui ZhangAbstract:Biomedical sciences, and in particular biomarker research, demand efficient glycoproteins enrichment platforms. In this work, we present a facile and time-saving method to synthesize phenylboronic acid and copolymer multiFunctionalized magnetic nanoparticles (NPs) using a distillation–precipitation polymerization (DPP) technique. The polymer shell is obtained through copolymerization of two Monomers-affinity ligand 3-acrylaminophenylboronic acid (AAPBA) and a hydrophilic Functional Monomer. The resulting hydrophilic Fe3O4@P(AAPBA-co-Monomer) NPs exhibit an enhanced binding capacity toward glycoproteins by an additional Functional Monomer complementary to the surface presentation of the target protein. The effects of Monomer ratio of AAPBA to hydrophilic coMonomers on the binding of glycoproteins are systematically investigated. The morphology, structure, and composition of all the synthesized microspheres are characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), Fourier ...
-
the preparation of bovine serum albumin surface imprinted superparamagnetic polymer with the assistance of basic Functional Monomer and its application for protein separation
Journal of Chromatography A, 2011Co-Authors: Feng Qu, Tao Zhang, Yukui ZhangAbstract:Currently, small proteins imprinting are more reported since large proteins molecular imprinting faces challenge due to their bulk size and complex structure. In this work, bovine serum albumin (BSA) surface-imprinted magnetic polymer was successfully synthesized based on atomic transfer radical polymerization (ATRP) method in the presence of common Monomer (N-isopropylacrylamide) with the assistant of basic Functional Monomer (N-[3-(dimethylamino)propyl]-methacrylamide), which provides a achievable attempt for imprinting larger target proteins based on the ATPR with the mild reaction conditions. The BSA-imprinted polymer exhibited higher adsorption capacity and selectivity to BSA over the non-imprinted polymer. Competitive adsorption tests indicated the BSA-imprinted polymer had better selective adsorption and recognition properties to BSA in the mixture. The obtained BSA-imprinted polymer was applied to bovine serum, which also showed selectivity to BSA. In addition, a conventional aqueous two-phase solution of PEG/sulphate was used as elution for adsorbed BSA, which was compared with common NaCl elution.
-
m aminophenylboronic acid as a Functional Monomer for fabricating molecularly imprinted polymer for the recognition of bovine serum albumin
Reactive & Functional Polymers, 2008Co-Authors: Huafang Wang, Xiwen He, Wenyou Li, Langxing Chen, Yukui ZhangAbstract:Abstract Using m -aminophenylboronic acid (APBA) as a Functional Monomer, molecularly imprinted polymer (MIP) imprinted with bovine serum albumin (BSA) was fabricated on activated glass spheres under optimized conditions. Key factors in the prepolymerization reaction (between APBA and BSA), such as buffer pH, ionic strength and reaction time, were carefully optimized as previously reported [H.F. Wang, W.Y. Li, X.W. He, et al. Acta Chim. Sinica 65 (2007) 43–48]. The interaction between APBA and BSA during the prepolymerization stage was investigated and optimized, and ideal conditions for protein rebinding experiments were determined as well. Protein rebinding and enriching properties of polymers were studied in single and competitive binding protocols, respectively. The key point of the present paper is that the binding selectivity of polymers may be estimated by the amount of bound-protein recovered from a protein-saturated polymer. Results demonstrated that the selectivity of MIP towards its template protein is superior to that of non-imprinted polymer (NIP).
Youcai Zhan - One of the best experts on this subject based on the ideXlab platform.
-
preparation of water compatible molecularly imprinted polymers for caffeine with a novel ionic liquid as a Functional Monomer
Journal of Applied Polymer Science, 2013Co-Authors: Ruizhi Dong, Youcai Zhan, Xinman Tu, Lixia YangAbstract:Water-compatible molecularly imprinted polymers (MIPs) for caffeine were synthesized in aqueous medium with a new Functional Monomer, 1-(α-methyl acrylate)-3-methylimidazolium bromide (1-MA-3MI-Br), which had π–π and hydrogen-bonding interactions. Caffeine-imprinted polymers were prepared in suspension polymerization with 1-MA-3MI-Br and methacrylic acid (MAA), which only had hydrogen bonding, as a Functional Monomer. For the specific binding characteristics of the new Functional Monomer 1-MA-3MI-Br, the adsorption capacity and relative separation factor (β) of MIPs for caffeine were significantly enhanced. The maximum adsorption capacities of 1-MA-3MI-Br–MIP and MAA–MIP imprinted microspheres for caffeine were 53.80 and 28.90 μmol/g, respectively. Moreover, the relative separation factors were measured by comparison of the separation characteristics under competitive adsorption conditions. The results showed that the β of MAA–MIP for caffeine relative to theophylline was only 1.65; this showed a very poor recognition selectivity for caffeine, but β of 1-MA-3MI-Br–MIP for caffeine with respect to theophylline was remarkably enhanced to 3.19; this showed an excellent recognition selectivity and binding affinity toward caffeine molecules in an aqueous environment. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
-
Preparation of water‐compatible molecularly imprinted polymers for caffeine with a novel ionic liquid as a Functional Monomer
Journal of Applied Polymer Science, 2012Co-Authors: Ruizhi Dong, Youcai Zhan, Xinman Tu, Lixia YangAbstract:Water-compatible molecularly imprinted polymers (MIPs) for caffeine were synthesized in aqueous medium with a new Functional Monomer, 1-(α-methyl acrylate)-3-methylimidazolium bromide (1-MA-3MI-Br), which had π–π and hydrogen-bonding interactions. Caffeine-imprinted polymers were prepared in suspension polymerization with 1-MA-3MI-Br and methacrylic acid (MAA), which only had hydrogen bonding, as a Functional Monomer. For the specific binding characteristics of the new Functional Monomer 1-MA-3MI-Br, the adsorption capacity and relative separation factor (β) of MIPs for caffeine were significantly enhanced. The maximum adsorption capacities of 1-MA-3MI-Br–MIP and MAA–MIP imprinted microspheres for caffeine were 53.80 and 28.90 μmol/g, respectively. Moreover, the relative separation factors were measured by comparison of the separation characteristics under competitive adsorption conditions. The results showed that the β of MAA–MIP for caffeine relative to theophylline was only 1.65; this showed a very poor recognition selectivity for caffeine, but β of 1-MA-3MI-Br–MIP for caffeine with respect to theophylline was remarkably enhanced to 3.19; this showed an excellent recognition selectivity and binding affinity toward caffeine molecules in an aqueous environment. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
-
novel molecularly imprinted polymer using 1 α methyl acrylate 3 methylimidazolium bromide as Functional Monomer for simultaneous extraction and determination of water soluble acid dyes in wastewater and soft drink by solid phase extraction and high p
Journal of Chromatography A, 2011Co-Authors: Youcai Zhan, Xinman Tu, Yining HuangAbstract:Abstract Novel water-compatible molecularly imprinted polymers were synthesized in methanol–water systems with Tratarzine as template and 1-(α-methyl acrylate)-3-methylimidazolium bromide (1-MA-3MI-Br) as Functional Monomer, which has π–π hydrophobic, hydrogen-bonding and electrostatic interactions with template molecule. 1-MA-3MI-Br molecularly imprinted polymers (1-MA-3MI-Br-MIPs) were used as selective sorbents for the solid-phase extraction (SPE) of water-soluble acid dyes from wastewater and soft drink. The good linearity of the method was obtained in a range of 5.0–2000 μg/L with the correlation coefficient of >0.999. The detection limits were in a range of 0.13–0.51 μg/L for the water-soluble acid dyes in wastewater and 0.095–0.84 μg/L for those in soft drink. The mean recoveries for the acid dyes are from 89.1% to 101.0% in spiked wastewater and 91.0–101.3% in spiked soft drink. Compared with strongly anion exchange solid phase extraction (SAX-SPE), mixture anion exchange solid phase extraction (MAX-SPE), and 1-MA-3MI-Br non-imprinted solid phase extraction (1-MA-3MI-Br-NISPE), almost all of the matrix interferences were removed by 1-MA-3MI-Br-MISPE, exhibiting higher selectivity, recovery and enrichment ability for the acid dyes and better baselines in the results of HPLC analysis.
Yining Huang - One of the best experts on this subject based on the ideXlab platform.
-
novel molecularly imprinted polymer using 1 α methyl acrylate 3 methylimidazolium bromide as Functional Monomer for simultaneous extraction and determination of water soluble acid dyes in wastewater and soft drink by solid phase extraction and high p
Journal of Chromatography A, 2011Co-Authors: Youcai Zhan, Xinman Tu, Yining HuangAbstract:Abstract Novel water-compatible molecularly imprinted polymers were synthesized in methanol–water systems with Tratarzine as template and 1-(α-methyl acrylate)-3-methylimidazolium bromide (1-MA-3MI-Br) as Functional Monomer, which has π–π hydrophobic, hydrogen-bonding and electrostatic interactions with template molecule. 1-MA-3MI-Br molecularly imprinted polymers (1-MA-3MI-Br-MIPs) were used as selective sorbents for the solid-phase extraction (SPE) of water-soluble acid dyes from wastewater and soft drink. The good linearity of the method was obtained in a range of 5.0–2000 μg/L with the correlation coefficient of >0.999. The detection limits were in a range of 0.13–0.51 μg/L for the water-soluble acid dyes in wastewater and 0.095–0.84 μg/L for those in soft drink. The mean recoveries for the acid dyes are from 89.1% to 101.0% in spiked wastewater and 91.0–101.3% in spiked soft drink. Compared with strongly anion exchange solid phase extraction (SAX-SPE), mixture anion exchange solid phase extraction (MAX-SPE), and 1-MA-3MI-Br non-imprinted solid phase extraction (1-MA-3MI-Br-NISPE), almost all of the matrix interferences were removed by 1-MA-3MI-Br-MISPE, exhibiting higher selectivity, recovery and enrichment ability for the acid dyes and better baselines in the results of HPLC analysis.
Xiwen He - One of the best experts on this subject based on the ideXlab platform.
-
tailor made boronic acid Functionalized magnetic nanoparticles with a tunable polymer shell assisted for the selective enrichment of glycoproteins glycopeptides
ACS Applied Materials & Interfaces, 2015Co-Authors: Xihao Zhang, Xiwen He, Langxing Chen, Jiewen Wang, Yukui ZhangAbstract:Biomedical sciences, and in particular biomarker research, demand efficient glycoproteins enrichment platforms. In this work, we present a facile and time-saving method to synthesize phenylboronic acid and copolymer multiFunctionalized magnetic nanoparticles (NPs) using a distillation–precipitation polymerization (DPP) technique. The polymer shell is obtained through copolymerization of two Monomers-affinity ligand 3-acrylaminophenylboronic acid (AAPBA) and a hydrophilic Functional Monomer. The resulting hydrophilic Fe3O4@P(AAPBA-co-Monomer) NPs exhibit an enhanced binding capacity toward glycoproteins by an additional Functional Monomer complementary to the surface presentation of the target protein. The effects of Monomer ratio of AAPBA to hydrophilic coMonomers on the binding of glycoproteins are systematically investigated. The morphology, structure, and composition of all the synthesized microspheres are characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), Fourier ...
-
preparation and application of lysozyme imprinted monolithic column with dopamine as the Functional Monomer
Journal of Materials Chemistry, 2012Co-Authors: Xiwen He, Wenyou LiAbstract:A novel protein imprinted monolithic column, which combines the merits of high selectivity of a molecular imprinting column and the new Functional Monomer dopamine, was prepared for selective separation of the template protein lysozyme (Lyz). The macroporous poly(GMA-DVB) skeleton was synthesized in a 4.6 mm i.d. stainless steel column with glycidyl methacrylate (GMA) and divinylbenzene (DVB) as precursors. Subsequently, the molecularly imprinted polymer (MIP) film coating with dopamine as the Functional Monomer and Lyz as imprinted template was noncovalently attached to the organic skeleton. The produced Lyz-MIP monoliths were characterized using high performance liquid chromatography (HPLC) analysis and exhibited excellent chromatographic performance and recognition ability. The imprinted factor (IF) reached 5.04. Furthermore, the MIP monolith was applied to separate the Lyz from chicken egg white with satisfactory results.
-
m aminophenylboronic acid as a Functional Monomer for fabricating molecularly imprinted polymer for the recognition of bovine serum albumin
Reactive & Functional Polymers, 2008Co-Authors: Huafang Wang, Xiwen He, Wenyou Li, Langxing Chen, Yukui ZhangAbstract:Abstract Using m -aminophenylboronic acid (APBA) as a Functional Monomer, molecularly imprinted polymer (MIP) imprinted with bovine serum albumin (BSA) was fabricated on activated glass spheres under optimized conditions. Key factors in the prepolymerization reaction (between APBA and BSA), such as buffer pH, ionic strength and reaction time, were carefully optimized as previously reported [H.F. Wang, W.Y. Li, X.W. He, et al. Acta Chim. Sinica 65 (2007) 43–48]. The interaction between APBA and BSA during the prepolymerization stage was investigated and optimized, and ideal conditions for protein rebinding experiments were determined as well. Protein rebinding and enriching properties of polymers were studied in single and competitive binding protocols, respectively. The key point of the present paper is that the binding selectivity of polymers may be estimated by the amount of bound-protein recovered from a protein-saturated polymer. Results demonstrated that the selectivity of MIP towards its template protein is superior to that of non-imprinted polymer (NIP).
Dapeng Wang - One of the best experts on this subject based on the ideXlab platform.
-
a surface Functional Monomer directing strategy for highly dense imprinting of tnt at surface of silica nanoparticles
Journal of the American Chemical Society, 2007Co-Authors: Zhongping Zhang, Minghong Wu, And Guijian Guan, Dapeng WangAbstract:This paper reports a surface Functional Monomer-directing strategy for the highly dense imprinting of 2,4,6-trinitrotoluene (TNT) molecules at the surface of silica nanoparticles. It has been demonstrated that the vinyl Functional Monomer layer of the silica surface can not only direct the selective occurrence of imprinting polymerization at the surface of silica through the copolymerization of vinyl end groups with Functional Monomers, but also drive TNT templates into the formed polymer shells through the charge-transfer complexing interactions between TNT and the Functional Monomer layer. The two basic processes lead to the formation of uniform core−shell TNT-imprinted nanoparticles with a controllable shell thickness and a high density of effective recognition sites. The high capacity and fast kinetics to uptake TNT molecules show that the density of effective imprinted sites in the nanoshells is nearly 5 times that of traditional imprinted particles. A critical value of shell thickness for the maximu...
