The Experts below are selected from a list of 1848 Experts worldwide ranked by ideXlab platform
Cameron R Currie - One of the best experts on this subject based on the ideXlab platform.
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cycloheximide producing streptomyces associated with xyleborinus saxesenii and xyleborus affinis fungus farming ambrosia beetles
Frontiers in Microbiology, 2020Co-Authors: Kirk J Grubbs, Jon Clardy, Frank Surup, Peter H W Biedermann, Bradon R Mcdonald, Jonathan L Klassen, Caitlin M Carlson, Cameron R CurrieAbstract:Symbiotic microbes help a myriad of insects acquire nutrients. Recent work suggests that insects also frequently associate with actinobacterial symbionts that produce molecules to help defend against parasites and predators. Here we explore a potential association between Actinobacteria and two species of fungus-farming ambrosia beetles, Xyleborinus saxesenii and Xyleborus affinis. We isolated and identified actinobacterial and Fungal symbionts from laboratory reared nests, and characterized small molecules produced by the putative actinobacterial symbionts. One 16S rRNA phylotype of Streptomyces (XylebKG-1) was abundantly and consistently isolated from the galleries and adults of X. saxesenii and X. affinis nests. In addition to Raffaelea sulphurea, the symbiont that X. saxesenii cultivates, we also repeatedly isolated a strain of Nectria sp. that is an Antagonist of this mutualism. Inhibition bioassays between Streptomyces griseus XylebKG-1 and the Fungal symbionts from X. saxesenii revealed strong inhibitory activity of the actinobacterium toward the Fungal Antagonist Nectria sp. but not the Fungal mutualist R. sulphurea. Bioassay guided HPLC fractionation of S. griseus XylebKG-1 culture extracts, followed by NMR and mass spectrometry, identified cycloheximide as the compound responsible for the observed growth inhibition. A biosynthetic gene cluster putatively encoding cycloheximide was also identified in S. griseus XylebKG-1. The consistent isolation of a single 16S phylotype of Streptomyces from two species of ambrosia beetles, and our finding that a representative isolate of this phylotype produces cycloheximide, which inhibits a parasite of the system but not the cultivated fungus, suggests that these actinobacteria may play defensive roles within these systems.
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cycloheximide producing streptomyces associated with xyleborinus saxesenii and xyleborus affinis fungus farming ambrosia beetles
bioRxiv, 2019Co-Authors: Kirk J Grubbs, Jon Clardy, Frank Surup, Peter H W Biedermann, Bradon R Mcdonald, Jonathan L Klassen, Caitlin M Carlson, Cameron R CurrieAbstract:Abstract Symbiotic microbes help a myriad of insects acquire nutrients. Recent work suggests that insects also frequently associate with actinobacterial symbionts that produce molecules to help defend against parasites and predators. Here we explore a potential association between Actinobacteria and two species of fungus-farming ambrosia beetles, Xyleborinus saxesenii and Xyleborus affinis. We isolated and identified actinobacterial and Fungal symbionts from laboratory reared nests, and characterized small molecules produced by the putative actinobacterial symbionts. One 16S rRNA phylotype of Streptomyces (XylebKG-1) was abundantly and consistently isolated from the nests and adults of X. saxesenii and X. affinis nests. In addition to Raffaelea sulphurea, the symbiont that X. saxesenii cultivates, we also repeatedly isolated a strain of Nectria sp. that is an Antagonist of this mutualism. Inhibition bioassays between S. griseus XylebKG-1 and the Fungal symbionts from X. saxesenii revealed strong inhibitory activity of the actinobacterium towards the Fungal Antagonist Nectria sp. but not the Fungal mutualist R. sulphurea. Bioassay guided HPLC fractionation of S. griseus XylebKG-1 culture extracts, followed by NMR and mass spectrometry identified cycloheximide as the compound responsible for the observed growth inhibition. A biosynthetic gene cluster putatively encoding cycloheximide was also identified in S. griseus XylebKG-1. The consistent isolation of a single 16S phylotype of Streptomyces from two species of ambrosia beetles, and our finding that a representative isolate of this phylotype produces cycloheximide, which inhibits a parasite of the system but not the cultivated fungus, suggests that these actinobacteria may play defensive roles within these systems.
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Bacterial protection of beetle-fungus mutualism
Science, 2008Co-Authors: Jarrod J. Scott, M. Cetin Yuceer, Kier D. Klepzig, Dong-chan Oh, Jon Clardy, Cameron R CurrieAbstract:Host-microbe symbioses play a critical role in the evolution of biological diversity and complexity. In a notably intricate system, southern pine beetles use symbiotic fungi to help overcome host-tree defenses and to provide nutrition for their larvae. We show that this beetle-Fungal mutualism is chemically mediated by a bacterially produced polyunsaturated peroxide. The molecule's selective toxicity toward the beetle's Fungal Antagonist, combined with the prevalence and localization of its bacterial source, indicates an insect-microbe association that is both mutualistic and coevolved. This unexpected finding in a well-studied system indicates that mutualistic associations between insects and antibiotic-producing bacteria are more common than currently recognized and that identifying their small-molecule mediators can provide a powerful search strategy for therapeutically useful antimicrobial compounds.
Kirk J Grubbs - One of the best experts on this subject based on the ideXlab platform.
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cycloheximide producing streptomyces associated with xyleborinus saxesenii and xyleborus affinis fungus farming ambrosia beetles
Frontiers in Microbiology, 2020Co-Authors: Kirk J Grubbs, Jon Clardy, Frank Surup, Peter H W Biedermann, Bradon R Mcdonald, Jonathan L Klassen, Caitlin M Carlson, Cameron R CurrieAbstract:Symbiotic microbes help a myriad of insects acquire nutrients. Recent work suggests that insects also frequently associate with actinobacterial symbionts that produce molecules to help defend against parasites and predators. Here we explore a potential association between Actinobacteria and two species of fungus-farming ambrosia beetles, Xyleborinus saxesenii and Xyleborus affinis. We isolated and identified actinobacterial and Fungal symbionts from laboratory reared nests, and characterized small molecules produced by the putative actinobacterial symbionts. One 16S rRNA phylotype of Streptomyces (XylebKG-1) was abundantly and consistently isolated from the galleries and adults of X. saxesenii and X. affinis nests. In addition to Raffaelea sulphurea, the symbiont that X. saxesenii cultivates, we also repeatedly isolated a strain of Nectria sp. that is an Antagonist of this mutualism. Inhibition bioassays between Streptomyces griseus XylebKG-1 and the Fungal symbionts from X. saxesenii revealed strong inhibitory activity of the actinobacterium toward the Fungal Antagonist Nectria sp. but not the Fungal mutualist R. sulphurea. Bioassay guided HPLC fractionation of S. griseus XylebKG-1 culture extracts, followed by NMR and mass spectrometry, identified cycloheximide as the compound responsible for the observed growth inhibition. A biosynthetic gene cluster putatively encoding cycloheximide was also identified in S. griseus XylebKG-1. The consistent isolation of a single 16S phylotype of Streptomyces from two species of ambrosia beetles, and our finding that a representative isolate of this phylotype produces cycloheximide, which inhibits a parasite of the system but not the cultivated fungus, suggests that these actinobacteria may play defensive roles within these systems.
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cycloheximide producing streptomyces associated with xyleborinus saxesenii and xyleborus affinis fungus farming ambrosia beetles
bioRxiv, 2019Co-Authors: Kirk J Grubbs, Jon Clardy, Frank Surup, Peter H W Biedermann, Bradon R Mcdonald, Jonathan L Klassen, Caitlin M Carlson, Cameron R CurrieAbstract:Abstract Symbiotic microbes help a myriad of insects acquire nutrients. Recent work suggests that insects also frequently associate with actinobacterial symbionts that produce molecules to help defend against parasites and predators. Here we explore a potential association between Actinobacteria and two species of fungus-farming ambrosia beetles, Xyleborinus saxesenii and Xyleborus affinis. We isolated and identified actinobacterial and Fungal symbionts from laboratory reared nests, and characterized small molecules produced by the putative actinobacterial symbionts. One 16S rRNA phylotype of Streptomyces (XylebKG-1) was abundantly and consistently isolated from the nests and adults of X. saxesenii and X. affinis nests. In addition to Raffaelea sulphurea, the symbiont that X. saxesenii cultivates, we also repeatedly isolated a strain of Nectria sp. that is an Antagonist of this mutualism. Inhibition bioassays between S. griseus XylebKG-1 and the Fungal symbionts from X. saxesenii revealed strong inhibitory activity of the actinobacterium towards the Fungal Antagonist Nectria sp. but not the Fungal mutualist R. sulphurea. Bioassay guided HPLC fractionation of S. griseus XylebKG-1 culture extracts, followed by NMR and mass spectrometry identified cycloheximide as the compound responsible for the observed growth inhibition. A biosynthetic gene cluster putatively encoding cycloheximide was also identified in S. griseus XylebKG-1. The consistent isolation of a single 16S phylotype of Streptomyces from two species of ambrosia beetles, and our finding that a representative isolate of this phylotype produces cycloheximide, which inhibits a parasite of the system but not the cultivated fungus, suggests that these actinobacteria may play defensive roles within these systems.
Jon Clardy - One of the best experts on this subject based on the ideXlab platform.
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cycloheximide producing streptomyces associated with xyleborinus saxesenii and xyleborus affinis fungus farming ambrosia beetles
Frontiers in Microbiology, 2020Co-Authors: Kirk J Grubbs, Jon Clardy, Frank Surup, Peter H W Biedermann, Bradon R Mcdonald, Jonathan L Klassen, Caitlin M Carlson, Cameron R CurrieAbstract:Symbiotic microbes help a myriad of insects acquire nutrients. Recent work suggests that insects also frequently associate with actinobacterial symbionts that produce molecules to help defend against parasites and predators. Here we explore a potential association between Actinobacteria and two species of fungus-farming ambrosia beetles, Xyleborinus saxesenii and Xyleborus affinis. We isolated and identified actinobacterial and Fungal symbionts from laboratory reared nests, and characterized small molecules produced by the putative actinobacterial symbionts. One 16S rRNA phylotype of Streptomyces (XylebKG-1) was abundantly and consistently isolated from the galleries and adults of X. saxesenii and X. affinis nests. In addition to Raffaelea sulphurea, the symbiont that X. saxesenii cultivates, we also repeatedly isolated a strain of Nectria sp. that is an Antagonist of this mutualism. Inhibition bioassays between Streptomyces griseus XylebKG-1 and the Fungal symbionts from X. saxesenii revealed strong inhibitory activity of the actinobacterium toward the Fungal Antagonist Nectria sp. but not the Fungal mutualist R. sulphurea. Bioassay guided HPLC fractionation of S. griseus XylebKG-1 culture extracts, followed by NMR and mass spectrometry, identified cycloheximide as the compound responsible for the observed growth inhibition. A biosynthetic gene cluster putatively encoding cycloheximide was also identified in S. griseus XylebKG-1. The consistent isolation of a single 16S phylotype of Streptomyces from two species of ambrosia beetles, and our finding that a representative isolate of this phylotype produces cycloheximide, which inhibits a parasite of the system but not the cultivated fungus, suggests that these actinobacteria may play defensive roles within these systems.
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cycloheximide producing streptomyces associated with xyleborinus saxesenii and xyleborus affinis fungus farming ambrosia beetles
bioRxiv, 2019Co-Authors: Kirk J Grubbs, Jon Clardy, Frank Surup, Peter H W Biedermann, Bradon R Mcdonald, Jonathan L Klassen, Caitlin M Carlson, Cameron R CurrieAbstract:Abstract Symbiotic microbes help a myriad of insects acquire nutrients. Recent work suggests that insects also frequently associate with actinobacterial symbionts that produce molecules to help defend against parasites and predators. Here we explore a potential association between Actinobacteria and two species of fungus-farming ambrosia beetles, Xyleborinus saxesenii and Xyleborus affinis. We isolated and identified actinobacterial and Fungal symbionts from laboratory reared nests, and characterized small molecules produced by the putative actinobacterial symbionts. One 16S rRNA phylotype of Streptomyces (XylebKG-1) was abundantly and consistently isolated from the nests and adults of X. saxesenii and X. affinis nests. In addition to Raffaelea sulphurea, the symbiont that X. saxesenii cultivates, we also repeatedly isolated a strain of Nectria sp. that is an Antagonist of this mutualism. Inhibition bioassays between S. griseus XylebKG-1 and the Fungal symbionts from X. saxesenii revealed strong inhibitory activity of the actinobacterium towards the Fungal Antagonist Nectria sp. but not the Fungal mutualist R. sulphurea. Bioassay guided HPLC fractionation of S. griseus XylebKG-1 culture extracts, followed by NMR and mass spectrometry identified cycloheximide as the compound responsible for the observed growth inhibition. A biosynthetic gene cluster putatively encoding cycloheximide was also identified in S. griseus XylebKG-1. The consistent isolation of a single 16S phylotype of Streptomyces from two species of ambrosia beetles, and our finding that a representative isolate of this phylotype produces cycloheximide, which inhibits a parasite of the system but not the cultivated fungus, suggests that these actinobacteria may play defensive roles within these systems.
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Bacterial protection of beetle-fungus mutualism
Science, 2008Co-Authors: Jarrod J. Scott, M. Cetin Yuceer, Kier D. Klepzig, Dong-chan Oh, Jon Clardy, Cameron R CurrieAbstract:Host-microbe symbioses play a critical role in the evolution of biological diversity and complexity. In a notably intricate system, southern pine beetles use symbiotic fungi to help overcome host-tree defenses and to provide nutrition for their larvae. We show that this beetle-Fungal mutualism is chemically mediated by a bacterially produced polyunsaturated peroxide. The molecule's selective toxicity toward the beetle's Fungal Antagonist, combined with the prevalence and localization of its bacterial source, indicates an insect-microbe association that is both mutualistic and coevolved. This unexpected finding in a well-studied system indicates that mutualistic associations between insects and antibiotic-producing bacteria are more common than currently recognized and that identifying their small-molecule mediators can provide a powerful search strategy for therapeutically useful antimicrobial compounds.
Frank Surup - One of the best experts on this subject based on the ideXlab platform.
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cycloheximide producing streptomyces associated with xyleborinus saxesenii and xyleborus affinis fungus farming ambrosia beetles
Frontiers in Microbiology, 2020Co-Authors: Kirk J Grubbs, Jon Clardy, Frank Surup, Peter H W Biedermann, Bradon R Mcdonald, Jonathan L Klassen, Caitlin M Carlson, Cameron R CurrieAbstract:Symbiotic microbes help a myriad of insects acquire nutrients. Recent work suggests that insects also frequently associate with actinobacterial symbionts that produce molecules to help defend against parasites and predators. Here we explore a potential association between Actinobacteria and two species of fungus-farming ambrosia beetles, Xyleborinus saxesenii and Xyleborus affinis. We isolated and identified actinobacterial and Fungal symbionts from laboratory reared nests, and characterized small molecules produced by the putative actinobacterial symbionts. One 16S rRNA phylotype of Streptomyces (XylebKG-1) was abundantly and consistently isolated from the galleries and adults of X. saxesenii and X. affinis nests. In addition to Raffaelea sulphurea, the symbiont that X. saxesenii cultivates, we also repeatedly isolated a strain of Nectria sp. that is an Antagonist of this mutualism. Inhibition bioassays between Streptomyces griseus XylebKG-1 and the Fungal symbionts from X. saxesenii revealed strong inhibitory activity of the actinobacterium toward the Fungal Antagonist Nectria sp. but not the Fungal mutualist R. sulphurea. Bioassay guided HPLC fractionation of S. griseus XylebKG-1 culture extracts, followed by NMR and mass spectrometry, identified cycloheximide as the compound responsible for the observed growth inhibition. A biosynthetic gene cluster putatively encoding cycloheximide was also identified in S. griseus XylebKG-1. The consistent isolation of a single 16S phylotype of Streptomyces from two species of ambrosia beetles, and our finding that a representative isolate of this phylotype produces cycloheximide, which inhibits a parasite of the system but not the cultivated fungus, suggests that these actinobacteria may play defensive roles within these systems.
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cycloheximide producing streptomyces associated with xyleborinus saxesenii and xyleborus affinis fungus farming ambrosia beetles
bioRxiv, 2019Co-Authors: Kirk J Grubbs, Jon Clardy, Frank Surup, Peter H W Biedermann, Bradon R Mcdonald, Jonathan L Klassen, Caitlin M Carlson, Cameron R CurrieAbstract:Abstract Symbiotic microbes help a myriad of insects acquire nutrients. Recent work suggests that insects also frequently associate with actinobacterial symbionts that produce molecules to help defend against parasites and predators. Here we explore a potential association between Actinobacteria and two species of fungus-farming ambrosia beetles, Xyleborinus saxesenii and Xyleborus affinis. We isolated and identified actinobacterial and Fungal symbionts from laboratory reared nests, and characterized small molecules produced by the putative actinobacterial symbionts. One 16S rRNA phylotype of Streptomyces (XylebKG-1) was abundantly and consistently isolated from the nests and adults of X. saxesenii and X. affinis nests. In addition to Raffaelea sulphurea, the symbiont that X. saxesenii cultivates, we also repeatedly isolated a strain of Nectria sp. that is an Antagonist of this mutualism. Inhibition bioassays between S. griseus XylebKG-1 and the Fungal symbionts from X. saxesenii revealed strong inhibitory activity of the actinobacterium towards the Fungal Antagonist Nectria sp. but not the Fungal mutualist R. sulphurea. Bioassay guided HPLC fractionation of S. griseus XylebKG-1 culture extracts, followed by NMR and mass spectrometry identified cycloheximide as the compound responsible for the observed growth inhibition. A biosynthetic gene cluster putatively encoding cycloheximide was also identified in S. griseus XylebKG-1. The consistent isolation of a single 16S phylotype of Streptomyces from two species of ambrosia beetles, and our finding that a representative isolate of this phylotype produces cycloheximide, which inhibits a parasite of the system but not the cultivated fungus, suggests that these actinobacteria may play defensive roles within these systems.
Peter H W Biedermann - One of the best experts on this subject based on the ideXlab platform.
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cycloheximide producing streptomyces associated with xyleborinus saxesenii and xyleborus affinis fungus farming ambrosia beetles
Frontiers in Microbiology, 2020Co-Authors: Kirk J Grubbs, Jon Clardy, Frank Surup, Peter H W Biedermann, Bradon R Mcdonald, Jonathan L Klassen, Caitlin M Carlson, Cameron R CurrieAbstract:Symbiotic microbes help a myriad of insects acquire nutrients. Recent work suggests that insects also frequently associate with actinobacterial symbionts that produce molecules to help defend against parasites and predators. Here we explore a potential association between Actinobacteria and two species of fungus-farming ambrosia beetles, Xyleborinus saxesenii and Xyleborus affinis. We isolated and identified actinobacterial and Fungal symbionts from laboratory reared nests, and characterized small molecules produced by the putative actinobacterial symbionts. One 16S rRNA phylotype of Streptomyces (XylebKG-1) was abundantly and consistently isolated from the galleries and adults of X. saxesenii and X. affinis nests. In addition to Raffaelea sulphurea, the symbiont that X. saxesenii cultivates, we also repeatedly isolated a strain of Nectria sp. that is an Antagonist of this mutualism. Inhibition bioassays between Streptomyces griseus XylebKG-1 and the Fungal symbionts from X. saxesenii revealed strong inhibitory activity of the actinobacterium toward the Fungal Antagonist Nectria sp. but not the Fungal mutualist R. sulphurea. Bioassay guided HPLC fractionation of S. griseus XylebKG-1 culture extracts, followed by NMR and mass spectrometry, identified cycloheximide as the compound responsible for the observed growth inhibition. A biosynthetic gene cluster putatively encoding cycloheximide was also identified in S. griseus XylebKG-1. The consistent isolation of a single 16S phylotype of Streptomyces from two species of ambrosia beetles, and our finding that a representative isolate of this phylotype produces cycloheximide, which inhibits a parasite of the system but not the cultivated fungus, suggests that these actinobacteria may play defensive roles within these systems.
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cycloheximide producing streptomyces associated with xyleborinus saxesenii and xyleborus affinis fungus farming ambrosia beetles
bioRxiv, 2019Co-Authors: Kirk J Grubbs, Jon Clardy, Frank Surup, Peter H W Biedermann, Bradon R Mcdonald, Jonathan L Klassen, Caitlin M Carlson, Cameron R CurrieAbstract:Abstract Symbiotic microbes help a myriad of insects acquire nutrients. Recent work suggests that insects also frequently associate with actinobacterial symbionts that produce molecules to help defend against parasites and predators. Here we explore a potential association between Actinobacteria and two species of fungus-farming ambrosia beetles, Xyleborinus saxesenii and Xyleborus affinis. We isolated and identified actinobacterial and Fungal symbionts from laboratory reared nests, and characterized small molecules produced by the putative actinobacterial symbionts. One 16S rRNA phylotype of Streptomyces (XylebKG-1) was abundantly and consistently isolated from the nests and adults of X. saxesenii and X. affinis nests. In addition to Raffaelea sulphurea, the symbiont that X. saxesenii cultivates, we also repeatedly isolated a strain of Nectria sp. that is an Antagonist of this mutualism. Inhibition bioassays between S. griseus XylebKG-1 and the Fungal symbionts from X. saxesenii revealed strong inhibitory activity of the actinobacterium towards the Fungal Antagonist Nectria sp. but not the Fungal mutualist R. sulphurea. Bioassay guided HPLC fractionation of S. griseus XylebKG-1 culture extracts, followed by NMR and mass spectrometry identified cycloheximide as the compound responsible for the observed growth inhibition. A biosynthetic gene cluster putatively encoding cycloheximide was also identified in S. griseus XylebKG-1. The consistent isolation of a single 16S phylotype of Streptomyces from two species of ambrosia beetles, and our finding that a representative isolate of this phylotype produces cycloheximide, which inhibits a parasite of the system but not the cultivated fungus, suggests that these actinobacteria may play defensive roles within these systems.
