The Experts below are selected from a list of 234 Experts worldwide ranked by ideXlab platform
Willem F. Broekaert - One of the best experts on this subject based on the ideXlab platform.
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a six cysteine type thionin from the radisch storage organ displays weak in vitro antifungal activity against Fusarium culmorum
Plant Physiology and Biochemistry, 1996Co-Authors: Franky R. G. Terras, Sophie Torrekens, Fred Van Leuven, Willem F. BroekaertAbstract:A 5 kDa peptide was purified from the basic protein fraction of radish (Raphanus sativus L.) storage organs. The amino-terminal amino acid sequence of this peptide (43 residues) shows homology with the subclass of thionins containing six cysteine residues of which crambin is a representative. The six-cysteine type thionin from radish displays in vitro antifungal activity against Fusarium culmorum. This activity is however substantially lower than that of the eight-cysteine type thionins from cereals.
J Perkowski - One of the best experts on this subject based on the ideXlab platform.
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response of some cultivars of spring spelt triticum spelta to Fusarium culmorum infection
2004Co-Authors: Marian Wiwart, J Perkowski, H Jackowiak, Danuta Packa, A BorusiewiczAbstract:Summary Five spring spelt (Triticum spelta) and two common wheat (T. aestivum) cultivars were examined for their response to Fusarium culmorum infection of heads and germinating kernels. In all objects inoculation significantly depressed values of the yield components analyzed: kernel weight, and kernel number per head and thousand kernel weight. Kernels from the control heads contained from 0.01 to 0.9 μg·g -1 deoxynivalenol whereas under artificial inoculation, the DON content of spelt kernels ranged from 33.7 to 108.4 μg·g -1 compared to 32.5 and 57.1 μg·g -1 in common wheat kernels. Germinating kernels and seedlings of T. spelta were characterized by better resistance to the infection relative to either of the two wheat cultivars.
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effects of head blight caused by Fusarium culmorum on toxin content and weight of wheat kernels
Phytopathology, 1990Co-Authors: C H A Snijders, J PerkowskiAbstract:Ten winter wheat genotypes were inoculated with three Dutch strains of Fusarium culmorum (IPO 39-01, IPO 348-01 and IPO 436-01). Seed samples were anlyzed for several trichothecene mycotoxins and zearalenone. Deoxynivalenol was detected in concentrations ranging from 0 to 48 mg/kg. Interactions between strains and genotypes were observed for head blight and kernel deoxynivalenol content. Path anlysis suggested a relation between deoxynivalenol and kernel weight reduction
Fiona M. Doohan - One of the best experts on this subject based on the ideXlab platform.
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Effect of temperature on head blight of wheat caused by Fusarium culmorum and F. graminearum
Plant Pathology, 2005Co-Authors: J. M. Brennan, Damian Egan, B. M. Cooke, Fiona M. DoohanAbstract:The effect of small temperature differentials (16 vs. 20°C) on the pathogenicity of deoxynivalenol producing single isolates of Fusarium culmorum and F. graminearum and on the Fusarium head blight (FHB) response of eight wheat cultivars was examined. Fusarium culmorum inoculation caused greater visual disease symptoms at 20°C than at 16°C, both overall and on an individual cultivar basis (overall AUDPC = 13·5 and 9·6, respectively) (P
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effect of temperature on head blight of wheat caused by Fusarium culmorum and f graminearum
Plant Pathology, 2005Co-Authors: J. M. Brennan, Damian Egan, B. M. Cooke, Fiona M. DoohanAbstract:The effect of small temperature differentials (16 vs. 20°C) on the pathogenicity of deoxynivalenol producing single isolates of Fusarium culmorum and F. graminearum and on the Fusarium head blight (FHB) response of eight wheat cultivars was examined. Fusarium culmorum inoculation caused greater visual disease symptoms at 20°C than at 16°C, both overall and on an individual cultivar basis (overall AUDPC = 13·5 and 9·6, respectively) (P < 0·05). In contrast, F. graminearum inoculation caused greater overall visual disease symptoms at 16°C than at 20°C, both overall and at the individual cultivar level (overall AUDPC = 12·8 and 10·9, respectively) (P < 0·05). Results showed both F. culmorum and F. graminearum inoculations caused a greater loss in yield at 20°C (54·3 and 46·9% relative 1000-grain weight, respectively) compared with 16°C (73·3 and 66·9% relative 1000-grain weight, respectively) (P < 0·05). Fusarium culmorum-inoculated heads contained similar amounts of fungal DNA at both 16 and 20°C (1·9 and 1·7 ng mg−1 of plant material, respectively) (not significant), while for F. graminearum inoculation, plants contained higher amounts of fungal DNA at 20°C (2·0 and 1·0 ng mg−1 of plant material, respectively) (P < 0·05). Overall, there was a significant negative correlation between AUDPC and percentage relative 1000-grain weight at both 16 and 20°C (r =−0·693 and −0·794, respectively, P < 0·01).
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Antifungal Activity Toward Fusarium culmorum in Soluble Wheat Extracts.
Phytopathology, 2000Co-Authors: Fiona M. Doohan, A. Mentewab, Paul NicholsonAbstract:ABSTRACT This study investigated antifungal activity in soluble extracts from seed of a range of wheat cultivars differing in susceptibility to Fusarium head blight. Antifungal activity was assessed in terms of β-D-glucuronidase (GUS) activity of a Fusarium culmorum GUS transformant using a sensitive laboratory assay. Significant antifungal activity was detected in seed extracts from WEK0609, CM 820036, and Arina. Initial characterization of the Arina seed extract indicated that it contained antifungal proteinaceous compounds. The Arina extract yielded two (60 and 80%) ammonium sulfate fractions containing inhibitory compounds. Gel filtration chromatography and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis of antifungal fractions showed that the antifungal activities detected in the Arina 60 and 80% ammonium sulfate fractions were associated with putative proteinaceous compounds with apparent molecular masses of approximately 60 and 28 kDa, respectively.
C H A Snijders - One of the best experts on this subject based on the ideXlab platform.
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Reaction of tritordeum to Fusarium culmorum and Septoria nodorum
Euphytica, 1996Co-Authors: D. Rubiales, P Nicholson, C H A Snijders, A. MartínAbstract:Hordeum chilense is a wild barley extensively used in wide crosses in the Triticeae . It could be a valuable source of resistance to Fusarium culmorum and Septoria nodorum . Some H. chilense x Triticum spp. amphiploids, named tritordeums, were more resistant than the parental wheat line to these diseases, others were not. Average contents of ergosterol and deoxynivalenol (DON) suggested that resistance to colonization by Fusarium was the highest for Hordeum chilense , followed by tritordeum and wheat in decreasing order. In particular, the H. chilense genotypes H7 and H17 enhanced the wheat resistance to F. culmorum in its tritordeum offsprings. Resistance to S. nodorum in tritordeum was not associated with tall plant height. There is sufficient genetic variation for resistance to F. culmorum and S. nodorum among tritordeum to allow the breeding of lines combining short straw and resistance to both diseases.
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systemic fungal growth of Fusarium culmorum in stems of winter wheat
Journal of Phytopathology, 1990Co-Authors: C H A SnijdersAbstract:Abstract Systemic fungal growth of Fusarium culmorum in winter wheat was investigated under conditions precluding secondary infections by water splash. Growth of F. culmorum in stem tissue was found in both wounded and soil inoculated plants with both methods resulting in a high level of infection. Crown rot can therefore lead to infection of the higher stem internodes under conditions not suitable for Fusarium dispersal. However, no evidence was found for systemic fungal growth leadingto infected heads. Existence of genetic variation for resistance to spread of F. culmorum in the host was found. This resistance was not correlated with resistance to Fusarium head blight.
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effects of head blight caused by Fusarium culmorum on toxin content and weight of wheat kernels
Phytopathology, 1990Co-Authors: C H A Snijders, J PerkowskiAbstract:Ten winter wheat genotypes were inoculated with three Dutch strains of Fusarium culmorum (IPO 39-01, IPO 348-01 and IPO 436-01). Seed samples were anlyzed for several trichothecene mycotoxins and zearalenone. Deoxynivalenol was detected in concentrations ranging from 0 to 48 mg/kg. Interactions between strains and genotypes were observed for head blight and kernel deoxynivalenol content. Path anlysis suggested a relation between deoxynivalenol and kernel weight reduction
Franky R. G. Terras - One of the best experts on this subject based on the ideXlab platform.
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a six cysteine type thionin from the radisch storage organ displays weak in vitro antifungal activity against Fusarium culmorum
Plant Physiology and Biochemistry, 1996Co-Authors: Franky R. G. Terras, Sophie Torrekens, Fred Van Leuven, Willem F. BroekaertAbstract:A 5 kDa peptide was purified from the basic protein fraction of radish (Raphanus sativus L.) storage organs. The amino-terminal amino acid sequence of this peptide (43 residues) shows homology with the subclass of thionins containing six cysteine residues of which crambin is a representative. The six-cysteine type thionin from radish displays in vitro antifungal activity against Fusarium culmorum. This activity is however substantially lower than that of the eight-cysteine type thionins from cereals.
