The Experts below are selected from a list of 6336 Experts worldwide ranked by ideXlab platform
Barbara Druml - One of the best experts on this subject based on the ideXlab platform.
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a novel reference real time pcr assay for the relative quantification of Game Meat species in raw and heat processed food
Food Control, 2016Co-Authors: Barbara Druml, Rupert Hochegger, Maria Kaltenbrunner, Margit CichnamarklAbstract:Abstract In order to increase their profit, food producers may be tempted to replace expensive Meat species by cheaper ones. Due to their selectivity and sensitivity, PCR based methods are frequently applied to identify and quantify Meat species to detect food adulteration. Quantification of Meat species in highly processed food products is, however, a difficult task. When we used a previously published reference system to relatively quantify the deer content in heat treated foodstuffs, we obtained recoveries that were substantially >100%. In the present study we aimed to improve the applicability of the reference system, targeting a 97 bp fragment of the myostatin gene, to heat treated products by reducing the length of the target sequence. The novel reference system, targeting a 70 bp fragment of the myostatin gene, was found to amplify the target region in 27 mammals and poultry species. Meat mixtures as well as raw and heat treated model sausages were analysed to demonstrate the applicability of the novel reference system for relatively quantifying the Game Meat content in processed food products. With the novel reference system, the bias introduced by heat treatment was largely eliminated.
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Duplex real-time PCR assay for the simultaneous determination of the roe deer (Capreolus capreolus) and deer (sum of fallow deer, red deer and sika deer) content in Game Meat products
Food Control, 2015Co-Authors: Barbara Druml, Rupert Hochegger, Margit Cichna-marklAbstract:Due to the high price of Game Meat, food producers may be tempted to adulterate their products with cheaper Meat. This paper presents a duplex real-time PCR assay which allows the simultaneous determination of the content of roe deer (Capreolus capreolus) and deer* (the sum of fallow deer (Dama dama), red deer (Cervus elaphus) and sika deer (Cervus nippon)) in food products to detect food adulteration. Relative quantification is carried out by using a reference (“all Meat”) PCR assay based on the myostatin gene. The quantification approach was validated by analyzing binary Meat mixtures with pork, “all Game” Meat mixtures containing each of the four Game species in pork and a model Game sausage. Compared to singleplex assays the duplex assay is time and cost saving. Thus, it is highly applicable to routine analysis in order to verify the authenticity of Game Meat products.
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authenticity control of Game Meat products a single method to detect and quantify adulteration of fallow deer dama dama red deer cervus elaphus and sika deer cervus nippon by real time pcr
Food Chemistry, 2015Co-Authors: Barbara Druml, Stephanie Grandits, Walter Mayer, Rupert Hochegger, Margit CichnamarklAbstract:This contribution presents a single real-time PCR assay allowing the determination of the deer content (the sum of fallow deer (Dama dama), red deer (Cervus elaphus) and sika deer (Cervus nippon)) in Meat products to detect food adulteration. The PCR assay does not show cross-reactivity with 20 animal species and 43 botanical species potentially contained in Game Meat products. The limit of quantification is 0.5% for fallow deer and red deer and 0.1% for sika deer. The deer content in Meat products is determined by relating the concentration obtained with the deer PCR assay to that obtained with a reference system which amplifies mammals and poultry DNA. The analysis of binary Meat mixtures with pork, a Meat mixture containing equal amounts of fallow deer, red deer and sika deer in pork and a model Game sausage showed that the quantification approach is very accurate (systematic error generally <25%).
Margit Cichnamarkl - One of the best experts on this subject based on the ideXlab platform.
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a novel reference real time pcr assay for the relative quantification of Game Meat species in raw and heat processed food
Food Control, 2016Co-Authors: Barbara Druml, Rupert Hochegger, Maria Kaltenbrunner, Margit CichnamarklAbstract:Abstract In order to increase their profit, food producers may be tempted to replace expensive Meat species by cheaper ones. Due to their selectivity and sensitivity, PCR based methods are frequently applied to identify and quantify Meat species to detect food adulteration. Quantification of Meat species in highly processed food products is, however, a difficult task. When we used a previously published reference system to relatively quantify the deer content in heat treated foodstuffs, we obtained recoveries that were substantially >100%. In the present study we aimed to improve the applicability of the reference system, targeting a 97 bp fragment of the myostatin gene, to heat treated products by reducing the length of the target sequence. The novel reference system, targeting a 70 bp fragment of the myostatin gene, was found to amplify the target region in 27 mammals and poultry species. Meat mixtures as well as raw and heat treated model sausages were analysed to demonstrate the applicability of the novel reference system for relatively quantifying the Game Meat content in processed food products. With the novel reference system, the bias introduced by heat treatment was largely eliminated.
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authenticity control of Game Meat products a single method to detect and quantify adulteration of fallow deer dama dama red deer cervus elaphus and sika deer cervus nippon by real time pcr
Food Chemistry, 2015Co-Authors: Barbara Druml, Stephanie Grandits, Walter Mayer, Rupert Hochegger, Margit CichnamarklAbstract:This contribution presents a single real-time PCR assay allowing the determination of the deer content (the sum of fallow deer (Dama dama), red deer (Cervus elaphus) and sika deer (Cervus nippon)) in Meat products to detect food adulteration. The PCR assay does not show cross-reactivity with 20 animal species and 43 botanical species potentially contained in Game Meat products. The limit of quantification is 0.5% for fallow deer and red deer and 0.1% for sika deer. The deer content in Meat products is determined by relating the concentration obtained with the deer PCR assay to that obtained with a reference system which amplifies mammals and poultry DNA. The analysis of binary Meat mixtures with pork, a Meat mixture containing equal amounts of fallow deer, red deer and sika deer in pork and a model Game sausage showed that the quantification approach is very accurate (systematic error generally <25%).
Rupert Hochegger - One of the best experts on this subject based on the ideXlab platform.
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a novel reference real time pcr assay for the relative quantification of Game Meat species in raw and heat processed food
Food Control, 2016Co-Authors: Barbara Druml, Rupert Hochegger, Maria Kaltenbrunner, Margit CichnamarklAbstract:Abstract In order to increase their profit, food producers may be tempted to replace expensive Meat species by cheaper ones. Due to their selectivity and sensitivity, PCR based methods are frequently applied to identify and quantify Meat species to detect food adulteration. Quantification of Meat species in highly processed food products is, however, a difficult task. When we used a previously published reference system to relatively quantify the deer content in heat treated foodstuffs, we obtained recoveries that were substantially >100%. In the present study we aimed to improve the applicability of the reference system, targeting a 97 bp fragment of the myostatin gene, to heat treated products by reducing the length of the target sequence. The novel reference system, targeting a 70 bp fragment of the myostatin gene, was found to amplify the target region in 27 mammals and poultry species. Meat mixtures as well as raw and heat treated model sausages were analysed to demonstrate the applicability of the novel reference system for relatively quantifying the Game Meat content in processed food products. With the novel reference system, the bias introduced by heat treatment was largely eliminated.
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Duplex real-time PCR assay for the simultaneous determination of the roe deer (Capreolus capreolus) and deer (sum of fallow deer, red deer and sika deer) content in Game Meat products
Food Control, 2015Co-Authors: Barbara Druml, Rupert Hochegger, Margit Cichna-marklAbstract:Due to the high price of Game Meat, food producers may be tempted to adulterate their products with cheaper Meat. This paper presents a duplex real-time PCR assay which allows the simultaneous determination of the content of roe deer (Capreolus capreolus) and deer* (the sum of fallow deer (Dama dama), red deer (Cervus elaphus) and sika deer (Cervus nippon)) in food products to detect food adulteration. Relative quantification is carried out by using a reference (“all Meat”) PCR assay based on the myostatin gene. The quantification approach was validated by analyzing binary Meat mixtures with pork, “all Game” Meat mixtures containing each of the four Game species in pork and a model Game sausage. Compared to singleplex assays the duplex assay is time and cost saving. Thus, it is highly applicable to routine analysis in order to verify the authenticity of Game Meat products.
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authenticity control of Game Meat products a single method to detect and quantify adulteration of fallow deer dama dama red deer cervus elaphus and sika deer cervus nippon by real time pcr
Food Chemistry, 2015Co-Authors: Barbara Druml, Stephanie Grandits, Walter Mayer, Rupert Hochegger, Margit CichnamarklAbstract:This contribution presents a single real-time PCR assay allowing the determination of the deer content (the sum of fallow deer (Dama dama), red deer (Cervus elaphus) and sika deer (Cervus nippon)) in Meat products to detect food adulteration. The PCR assay does not show cross-reactivity with 20 animal species and 43 botanical species potentially contained in Game Meat products. The limit of quantification is 0.5% for fallow deer and red deer and 0.1% for sika deer. The deer content in Meat products is determined by relating the concentration obtained with the deer PCR assay to that obtained with a reference system which amplifies mammals and poultry DNA. The analysis of binary Meat mixtures with pork, a Meat mixture containing equal amounts of fallow deer, red deer and sika deer in pork and a model Game sausage showed that the quantification approach is very accurate (systematic error generally <25%).
Rosario Martin - One of the best experts on this subject based on the ideXlab platform.
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pcr rflp authentication of Meats from red deer cervus elaphus fallow deer dama dama roe deer capreolus capreolus cattle bos taurus sheep ovis aries and goat capra hircus
Journal of Agricultural and Food Chemistry, 2006Co-Authors: Violeta Fajardo, Isabel Gonzalez, Ines Lopezcalleja, Irene Martinez Martin, Pablo E Hernandez, Teresa Garcia, Rosario MartinAbstract:PCR-RFLP analysis has been applied to the identification of Meats from red deer (Cervus elaphus), fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries), and goat (Capra hircus). PCR amplification was carried out using a set of primers flanking a conserved region of ∼712 base pairs from the mitochondrial 12S rRNA gene. Restriction site analysis based on sequence data from this DNA fragment permitted the selection of MseI, MboII, BslI, and ApoI endonucleases for species identification. The restriction profiles obtained when amplicons were digested with the chosen enzymes allowed the unequivocal identification of all domestic and Game Meat species analyzed in the present work. Keywords: Game Meat; species identification; 12S rRNA gene; PCR-RFLP
Joana S Amaral - One of the best experts on this subject based on the ideXlab platform.
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identification of duck partridge pheasant quail chicken and turkey Meats by species specific pcr assays to assess the authenticity of traditional Game Meat alheira sausages
Food Control, 2015Co-Authors: Joana S Amaral, Cristina Santos, Vitor S Melo, Joana Costa, Beatriz M P P Oliveira, Isabel MafraAbstract:Abstract Game Meat Alheira ( Alheira de caca ) sausage is a traditional fermented product typical from the Northeast region of Portugal, having bread and Meats (including Game) as main ingredients. It is a particularly appreciated product by consumers that commands higher prices, especially in comparison with the common Alheira produced with pork and poultry Meats. Following our previous work in which several mammalian Game Meat species were successfully identified in Game Meat Alheira sausages for authentication purposes, the present work aimed at identifying Game bird's species for the overall assessment of labelling compliance. For that purpose, several species-specific PCR assays targeting mitochondrial DNA for the detection of Game and domestic bird's Meat, namely duck, partridge, pheasant, quail, chicken and turkey were developed, optimised and applied to commercial samples of Game Meat Alheira for their authentication. The assays revealed a high specificity and sensitivity to detect the addition of all evaluated species down to a level of 0.01% (w/w). PCR results indicated the existence of several inconsistencies with the labelled information, namely the absence of declared Game species (duck, partridge and pheasant) and the presence of undeclared poultry Meat, pointing out to adulterations owing to substitution of Game by domestic Meat species. Since this is considered a high-valued traditional product that should be valorised and protected, this work puts in evidence the need for inspection programs to enforce regulation.
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authentication of a traditional Game Meat sausage alheira by species specific pcr assays to detect hare rabbit red deer pork and cow Meats
Food Research International, 2014Co-Authors: Joana S Amaral, Cristina Santos, Vitor S Melo, Beatriz M P P Oliveira, Isabel MafraAbstract:Alheira is a traditional Meat product that is typical from the Northeast region of Portugal and much appreciated. It is a sort of sausage produced industrially or by small artisanal producers, having wheat bread and Meats as main ingredients. Game Meat Alheira (Alheira de caca) is considered one of the most attractive products since it should include different Game Meats. The aim of the present work was to identify the species of origin of Meats added to Game Meat Alheira samples to verify their compliance with labelling. Species-specific PCR assays targeting mitochondrial genes of rabbit, hare, red deer, cow and pork were optimised and applied to industrial and artisanal samples. The assays revealed adequate specificity for each of the targeted species, with sensitivities of 0.01–0.1%. Results of the evaluation of 18 commercial samples identified several inconsistencies with labelling, namely the absence of declared Game species (red deer, hare and rabbit) in ten samples and the presence of undeclared cow species in nine of the analysed samples. These findings indicate the occurrence of misleading labelling, suggesting the adulteration by substitution of Game Meats by cow Meat to reduce production costs and the need to protect and valorise this kind of traditional food product.
