The Experts below are selected from a list of 564 Experts worldwide ranked by ideXlab platform
Hadi Hamishehkar - One of the best experts on this subject based on the ideXlab platform.
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Polycaprolactone nanofiber coated with chitosan and Gamma Oryzanol functionalized as a novel wound dressing for healing infected wounds.
International journal of biological macromolecules, 2020Co-Authors: Hesaam Hajilou, Mohammad Reza Farahpour, Hadi HamishehkarAbstract:Abstract This study was conducted to design and evaluate a wound dressing based on a polycaprolactone (PCL) nanofiber coated with Gamma Oryzanol (GO) and chitosan (CS) in mice model. All the dressings were prepared by electrospinning method, and their morphology and physical properties were investigated. The mice were divided into five groups and treated with I) PCL-sole (PCL), III) PCL-mupirocin (PCL-M), IV) PCL-GO, IV) PCL-CS, and V) PCL-CS-GO. Wound area, total bacterial count, histopathological parameters, and expressions of IL-1β, TNF-α, IL-10, MMP-9, EGF, and VEGF were assessed. The fibers were randomly distributed in PCL group, but loading CS and GO increased the complexity and placing on the dressings. PCLs loaded with GO and CS showed lower viscosity, surface tension, and fiber diameter and higher conductivity and water contact angle compared to unloaded PCLs (P
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evaluation of Gamma Oryzanol effects on oxidative stress factors in animal model of sepsis
2019Co-Authors: Elmira Zolali, Hadi HamishehkarAbstract:Evaluation of Gamma Oryzanol effects on oxidative stress factors in animal model of sepsis , Evaluation of Gamma Oryzanol effects on oxidative stress factors in animal model of sepsis , بانک اطلاعاتی جامع اطلاع رسانی پایان نامه های کشور
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effects of Gamma Oryzanol on factors of oxidative stress and sepsis induced lung injury in experimental animal model
Iranian Journal of Basic Medical Sciences, 2015Co-Authors: Elmira Zolali, Hadi Hamishehkar, Parina Asgharian, Maryam Kouhsoltani, Hajhir KhodaiiAbstract:Objective (s): There is corroborating evidence to substantiate redox imbalance and oxidative stress in sepsis that finally leads to organ damage or even death. Gamma Oryzanol (GO) is one of the major bioactive components in rice bran has been considered to function as an antioxidant. The present study was carried out to evaluate the antioxidant activity of Gamma Oryzanol in vitro and its efficacy in sepsis. Materials and Methods: To induce sepsis, cecal ligation and puncture (CLP) method was performed on the rats. A study group of forty male Wistar rats were divided into the following groups: sham group; CLP group; 50 mg/kg GO- treated CLP group and 100 mg/kg GO- treated CLP group. GO was administered with an oral gavage 2 hr prior to inducing sepsis. Tissue and blood samples were collected 12 hr after CLP to prepare tissue sections for histopathological study and assay the oxidative stress biomarkers including: SOD (Superoxide Dismutase), TAC (total antioxidant capacity), MDA (Malondialdehyde), MPO (Myeloperoxidase) and PAI-1 (Plasminogen Activator Inhibitor-1). Data are given as mean ± SD. The ANOVA with Tukey post hoc test was used to determine the differences between groups and P Results: TAC level increased in GO- treated CLP groups (P<0.05). Inflammation score of lung tissue and MPO activity were significantly lower in GO treated CLP group (P<0.05). Conclusion:It seems that GO has a protective effect on lung inflammation and improves the body redox capacity during sepsis.
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Effects of Gamma Oryzanol on factors of oxidative stress and sepsis-induced lung injury in experimental animal model
Iranian journal of basic medical sciences, 2015Co-Authors: Elmira Zolali, Hadi Hamishehkar, Parina Asgharian, Maryam Kouhsoltani, Hajhir KhodaiiAbstract:Objective (s): There is corroborating evidence to substantiate redox imbalance and oxidative stress in sepsis that finally leads to organ damage or even death. Gamma Oryzanol (GO) is one of the major bioactive components in rice bran has been considered to function as an antioxidant. The present study was carried out to evaluate the antioxidant activity of Gamma Oryzanol in vitro and its efficacy in sepsis. Materials and Methods: To induce sepsis, cecal ligation and puncture (CLP) method was performed on the rats. A study group of forty male Wistar rats were divided into the following groups: sham group; CLP group; 50 mg/kg GO- treated CLP group and 100 mg/kg GO- treated CLP group. GO was administered with an oral gavage 2 hr prior to inducing sepsis. Tissue and blood samples were collected 12 hr after CLP to prepare tissue sections for histopathological study and assay the oxidative stress biomarkers including: SOD (Superoxide Dismutase), TAC (total antioxidant capacity), MDA (Malondialdehyde), MPO (Myeloperoxidase) and PAI-1 (Plasminogen Activator Inhibitor-1). Data are given as mean ± SD. The ANOVA with Tukey post hoc test was used to determine the differences between groups and P Results: TAC level increased in GO- treated CLP groups (P
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Effects of Gamma Oryzanol on oxidative stress factors in animal model of sepsis
2014Co-Authors: Elmira Zolali, Hadi Hamishehkar, Nasrin Maleki-dizajiAbstract:There is corroborating evidence to substantiate redox imbalance and oxidative stress in sepsis that finally leads to organ damage or even death. Gamma Oryzanol (GO) is one of the major bioactive components in rice bran has been considered to function as an antioxidant. To investigate the antioxidative effects of GO on oxidative stress factors in septic animals. To induce sepsis, cecal ligation and puncture (CLP) method was performed on the rats. A study group of forty male Wistar rats were divided into the following groups: sham group; CLP group; 50 mg/kg GO- treated CLP group and 100 mg/kg GO- treated CLP group. GO was administered with an oral gavage 2 hour prior to inducing sepsis. Blood and tissue samples were collected 12 hours after CLP to assay the oxidative stress biomarkers and prepare tissue sections for histopathological study. Whole blood total antioxidant capacity (TAC) level increased in GO- treated CLP groups. Inflammation score of lung tissue and Myeloperoxidase (MPO) activity were significantly lower in GO treated CLP group. Glutathione peroxidise (GPx) level of lung tissue was improved by treatment with GO. It seems that GO has a protective effect on lung inflammation and improves the body redox capacity during sepsis.
T. Sadkowski - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide
Nutrients, 2018Co-Authors: Karolina A. Chodkowska, A. Ciecierska, P. Ostaszewski, Kinga Majchrzak, T. SadkowskiAbstract:Gamma-Oryzanol (GO) is a popular supplement for performance horses, dogs, and humans. Previous studies indicated that GO supplementation decreases creatine kinase activity and lactate level after exercise and may affect oxidative stress in Thoroughbred horses. GO may change genes expression in equine satellite cells (ESC). The purpose of this study was to evaluate the effect of GO on miRNA, gene expression, oxidative stress, and cell damage and viability in differentiating ESC pretreated with hydrogen peroxide (H2O2). ESCs were obtained from a young horse’s skeletal muscle. ESCs were pre-incubated with GO (24 h) and then exposed to H2O2 for one hour. For the microRNA and gene expression assessment, the microarray technique was used. Identified miRNAs and genes were validated using real time-quantitative polymerase chain reaction. Several tests related to cell viability, cell damage, and oxidative stress were performed. The microarray analysis revealed differences in 17 miRNAs and 202 genes between GO-treated and control ESC. The tests related to apoptosis, cell viability, and oxidative stress showed that GO affects these processes to varying degrees. Our results suggest that GO can change miRNA and gene expression and may impact the processes involved in tissue repairing after an injury.
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investigation of nutriactive phytochemical Gamma Oryzanol in experimental animal models
Journal of Animal Physiology and Animal Nutrition, 2016Co-Authors: Katarzyna A. Szcześniak, A. Ciecierska, P. Ostaszewski, T. SadkowskiAbstract:Gamma-Oryzanol (GO) is an abundant dietary antioxidant that is considered to have beneficial effects in cardiovascular disease, cancer and diabetes. Other potential properties of GO include inhibition of gastric acid secretion and decreased post-exercise muscle fatigue. GO is a unique mixture of triterpene alcohol and sterol ferulates present in rice bran oil, a byproduct of rice processing. GO has been studied by many researchers over the last three decades. In particular, the utility of GO supplementation has been documented in numerous animal models. A large variety of species was examined, and various experimental methodologies and targets were applied. The aim of this study was to summarize the body of research on GO supplementation in animals and to examine possible mechanisms of GO action. Furthermore, while the safety of GO supplementation in animals has been well documented, studies demonstrating pharmacokinetics, pharmacodynamics and efficiency are less clear. The observed differences in these findings are also discussed.
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Transcriptomic profile adaptations following exposure of equine satellite cells to nutriactive phytochemical Gamma-Oryzanol.
Genes & nutrition, 2016Co-Authors: K. A. Szcześniak, A. Ciecierska, P. Ostaszewski, T. SadkowskiAbstract:Background Adult skeletal muscle myogenesis depends on the activation of satellite cells that have the potential to differentiate into new fibers. Gamma-Oryzanol (GO), a commercially available nutriactive phytochemical, has gained global interest on account of its muscle-building and regenerating effects. Here, we investigated GO for its potential influence on myogenesis, using equine satellite cell culture model, since the horse is a unique animal, bred and exercised for competitive sport. To our knowledge, this is the first report where the global gene expression in cultured equine satellite cells has been described.
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Transcriptomic profile adaptations following exposure of equine satellite cells to nutriactive phytochemical Gamma-Oryzanol
Genes & Nutrition, 2016Co-Authors: K. A. Szcześniak, A. Ciecierska, P. Ostaszewski, T. SadkowskiAbstract:Background Adult skeletal muscle myogenesis depends on the activation of satellite cells that have the potential to differentiate into new fibers. Gamma-Oryzanol (GO), a commercially available nutriactive phytochemical, has gained global interest on account of its muscle-building and regenerating effects. Here, we investigated GO for its potential influence on myogenesis, using equine satellite cell culture model, since the horse is a unique animal, bred and exercised for competitive sport. To our knowledge, this is the first report where the global gene expression in cultured equine satellite cells has been described. Methods Equine satellite cells were isolated from semitendinosus muscle and cultured until the second day of differentiation. Differentiating cells were incubated with GO for the next 24 h. Subsequently, total RNA from GO-treated and control cells was isolated, amplified, labeled, and hybridized to two-color Horse Gene Expression Microarray slides. Quantitative PCR was used for the validation of microarray data. Results Our results revealed 58 genes with changed expression in GO-treated vs. control cells. Analysis of expression changes suggests that various processes are reinforced by GO in differentiating equine satellite cells, including inhibition of myoblast differentiation, increased proliferation and differentiation, stress response, and increased myogenic lineage commitment. Conclusions The present study may confirm putative muscle-enhancing abilities of GO; however, the collective role of GO in skeletal myogenesis remains equivocal. The diversity of these changes is likely due to heterogenous growth rate of cells in primary culture. Genes identified in our study, modulated by the presence of GO, may become potential targets of future research investigating impact of this supplement in skeletal muscle on proteomic and biochemical level.
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Additional file 1: of Transcriptomic profile adaptations following exposure of equine satellite cells to nutriactive phytochemical Gamma-Oryzanol
2016Co-Authors: K. SzczeĹniak, A. Ciecierska, P. Ostaszewski, T. SadkowskiAbstract:Full list of differentially expressed transcripts, Gamma-Oryzanol vs. control. (XLS 55.0 kb
P. Ostaszewski - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide
Nutrients, 2018Co-Authors: Karolina A. Chodkowska, A. Ciecierska, P. Ostaszewski, Kinga Majchrzak, T. SadkowskiAbstract:Gamma-Oryzanol (GO) is a popular supplement for performance horses, dogs, and humans. Previous studies indicated that GO supplementation decreases creatine kinase activity and lactate level after exercise and may affect oxidative stress in Thoroughbred horses. GO may change genes expression in equine satellite cells (ESC). The purpose of this study was to evaluate the effect of GO on miRNA, gene expression, oxidative stress, and cell damage and viability in differentiating ESC pretreated with hydrogen peroxide (H2O2). ESCs were obtained from a young horse’s skeletal muscle. ESCs were pre-incubated with GO (24 h) and then exposed to H2O2 for one hour. For the microRNA and gene expression assessment, the microarray technique was used. Identified miRNAs and genes were validated using real time-quantitative polymerase chain reaction. Several tests related to cell viability, cell damage, and oxidative stress were performed. The microarray analysis revealed differences in 17 miRNAs and 202 genes between GO-treated and control ESC. The tests related to apoptosis, cell viability, and oxidative stress showed that GO affects these processes to varying degrees. Our results suggest that GO can change miRNA and gene expression and may impact the processes involved in tissue repairing after an injury.
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investigation of nutriactive phytochemical Gamma Oryzanol in experimental animal models
Journal of Animal Physiology and Animal Nutrition, 2016Co-Authors: Katarzyna A. Szcześniak, A. Ciecierska, P. Ostaszewski, T. SadkowskiAbstract:Gamma-Oryzanol (GO) is an abundant dietary antioxidant that is considered to have beneficial effects in cardiovascular disease, cancer and diabetes. Other potential properties of GO include inhibition of gastric acid secretion and decreased post-exercise muscle fatigue. GO is a unique mixture of triterpene alcohol and sterol ferulates present in rice bran oil, a byproduct of rice processing. GO has been studied by many researchers over the last three decades. In particular, the utility of GO supplementation has been documented in numerous animal models. A large variety of species was examined, and various experimental methodologies and targets were applied. The aim of this study was to summarize the body of research on GO supplementation in animals and to examine possible mechanisms of GO action. Furthermore, while the safety of GO supplementation in animals has been well documented, studies demonstrating pharmacokinetics, pharmacodynamics and efficiency are less clear. The observed differences in these findings are also discussed.
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Transcriptomic profile adaptations following exposure of equine satellite cells to nutriactive phytochemical Gamma-Oryzanol.
Genes & nutrition, 2016Co-Authors: K. A. Szcześniak, A. Ciecierska, P. Ostaszewski, T. SadkowskiAbstract:Background Adult skeletal muscle myogenesis depends on the activation of satellite cells that have the potential to differentiate into new fibers. Gamma-Oryzanol (GO), a commercially available nutriactive phytochemical, has gained global interest on account of its muscle-building and regenerating effects. Here, we investigated GO for its potential influence on myogenesis, using equine satellite cell culture model, since the horse is a unique animal, bred and exercised for competitive sport. To our knowledge, this is the first report where the global gene expression in cultured equine satellite cells has been described.
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Transcriptomic profile adaptations following exposure of equine satellite cells to nutriactive phytochemical Gamma-Oryzanol
Genes & Nutrition, 2016Co-Authors: K. A. Szcześniak, A. Ciecierska, P. Ostaszewski, T. SadkowskiAbstract:Background Adult skeletal muscle myogenesis depends on the activation of satellite cells that have the potential to differentiate into new fibers. Gamma-Oryzanol (GO), a commercially available nutriactive phytochemical, has gained global interest on account of its muscle-building and regenerating effects. Here, we investigated GO for its potential influence on myogenesis, using equine satellite cell culture model, since the horse is a unique animal, bred and exercised for competitive sport. To our knowledge, this is the first report where the global gene expression in cultured equine satellite cells has been described. Methods Equine satellite cells were isolated from semitendinosus muscle and cultured until the second day of differentiation. Differentiating cells were incubated with GO for the next 24 h. Subsequently, total RNA from GO-treated and control cells was isolated, amplified, labeled, and hybridized to two-color Horse Gene Expression Microarray slides. Quantitative PCR was used for the validation of microarray data. Results Our results revealed 58 genes with changed expression in GO-treated vs. control cells. Analysis of expression changes suggests that various processes are reinforced by GO in differentiating equine satellite cells, including inhibition of myoblast differentiation, increased proliferation and differentiation, stress response, and increased myogenic lineage commitment. Conclusions The present study may confirm putative muscle-enhancing abilities of GO; however, the collective role of GO in skeletal myogenesis remains equivocal. The diversity of these changes is likely due to heterogenous growth rate of cells in primary culture. Genes identified in our study, modulated by the presence of GO, may become potential targets of future research investigating impact of this supplement in skeletal muscle on proteomic and biochemical level.
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Additional file 1: of Transcriptomic profile adaptations following exposure of equine satellite cells to nutriactive phytochemical Gamma-Oryzanol
2016Co-Authors: K. SzczeĹniak, A. Ciecierska, P. Ostaszewski, T. SadkowskiAbstract:Full list of differentially expressed transcripts, Gamma-Oryzanol vs. control. (XLS 55.0 kb
A. Ciecierska - One of the best experts on this subject based on the ideXlab platform.
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Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide
Nutrients, 2018Co-Authors: Karolina A. Chodkowska, A. Ciecierska, P. Ostaszewski, Kinga Majchrzak, T. SadkowskiAbstract:Gamma-Oryzanol (GO) is a popular supplement for performance horses, dogs, and humans. Previous studies indicated that GO supplementation decreases creatine kinase activity and lactate level after exercise and may affect oxidative stress in Thoroughbred horses. GO may change genes expression in equine satellite cells (ESC). The purpose of this study was to evaluate the effect of GO on miRNA, gene expression, oxidative stress, and cell damage and viability in differentiating ESC pretreated with hydrogen peroxide (H2O2). ESCs were obtained from a young horse’s skeletal muscle. ESCs were pre-incubated with GO (24 h) and then exposed to H2O2 for one hour. For the microRNA and gene expression assessment, the microarray technique was used. Identified miRNAs and genes were validated using real time-quantitative polymerase chain reaction. Several tests related to cell viability, cell damage, and oxidative stress were performed. The microarray analysis revealed differences in 17 miRNAs and 202 genes between GO-treated and control ESC. The tests related to apoptosis, cell viability, and oxidative stress showed that GO affects these processes to varying degrees. Our results suggest that GO can change miRNA and gene expression and may impact the processes involved in tissue repairing after an injury.
-
investigation of nutriactive phytochemical Gamma Oryzanol in experimental animal models
Journal of Animal Physiology and Animal Nutrition, 2016Co-Authors: Katarzyna A. Szcześniak, A. Ciecierska, P. Ostaszewski, T. SadkowskiAbstract:Gamma-Oryzanol (GO) is an abundant dietary antioxidant that is considered to have beneficial effects in cardiovascular disease, cancer and diabetes. Other potential properties of GO include inhibition of gastric acid secretion and decreased post-exercise muscle fatigue. GO is a unique mixture of triterpene alcohol and sterol ferulates present in rice bran oil, a byproduct of rice processing. GO has been studied by many researchers over the last three decades. In particular, the utility of GO supplementation has been documented in numerous animal models. A large variety of species was examined, and various experimental methodologies and targets were applied. The aim of this study was to summarize the body of research on GO supplementation in animals and to examine possible mechanisms of GO action. Furthermore, while the safety of GO supplementation in animals has been well documented, studies demonstrating pharmacokinetics, pharmacodynamics and efficiency are less clear. The observed differences in these findings are also discussed.
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Transcriptomic profile adaptations following exposure of equine satellite cells to nutriactive phytochemical Gamma-Oryzanol.
Genes & nutrition, 2016Co-Authors: K. A. Szcześniak, A. Ciecierska, P. Ostaszewski, T. SadkowskiAbstract:Background Adult skeletal muscle myogenesis depends on the activation of satellite cells that have the potential to differentiate into new fibers. Gamma-Oryzanol (GO), a commercially available nutriactive phytochemical, has gained global interest on account of its muscle-building and regenerating effects. Here, we investigated GO for its potential influence on myogenesis, using equine satellite cell culture model, since the horse is a unique animal, bred and exercised for competitive sport. To our knowledge, this is the first report where the global gene expression in cultured equine satellite cells has been described.
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Transcriptomic profile adaptations following exposure of equine satellite cells to nutriactive phytochemical Gamma-Oryzanol
Genes & Nutrition, 2016Co-Authors: K. A. Szcześniak, A. Ciecierska, P. Ostaszewski, T. SadkowskiAbstract:Background Adult skeletal muscle myogenesis depends on the activation of satellite cells that have the potential to differentiate into new fibers. Gamma-Oryzanol (GO), a commercially available nutriactive phytochemical, has gained global interest on account of its muscle-building and regenerating effects. Here, we investigated GO for its potential influence on myogenesis, using equine satellite cell culture model, since the horse is a unique animal, bred and exercised for competitive sport. To our knowledge, this is the first report where the global gene expression in cultured equine satellite cells has been described. Methods Equine satellite cells were isolated from semitendinosus muscle and cultured until the second day of differentiation. Differentiating cells were incubated with GO for the next 24 h. Subsequently, total RNA from GO-treated and control cells was isolated, amplified, labeled, and hybridized to two-color Horse Gene Expression Microarray slides. Quantitative PCR was used for the validation of microarray data. Results Our results revealed 58 genes with changed expression in GO-treated vs. control cells. Analysis of expression changes suggests that various processes are reinforced by GO in differentiating equine satellite cells, including inhibition of myoblast differentiation, increased proliferation and differentiation, stress response, and increased myogenic lineage commitment. Conclusions The present study may confirm putative muscle-enhancing abilities of GO; however, the collective role of GO in skeletal myogenesis remains equivocal. The diversity of these changes is likely due to heterogenous growth rate of cells in primary culture. Genes identified in our study, modulated by the presence of GO, may become potential targets of future research investigating impact of this supplement in skeletal muscle on proteomic and biochemical level.
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Additional file 1: of Transcriptomic profile adaptations following exposure of equine satellite cells to nutriactive phytochemical Gamma-Oryzanol
2016Co-Authors: K. SzczeĹniak, A. Ciecierska, P. Ostaszewski, T. SadkowskiAbstract:Full list of differentially expressed transcripts, Gamma-Oryzanol vs. control. (XLS 55.0 kb
Elmira Zolali - One of the best experts on this subject based on the ideXlab platform.
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evaluation of Gamma Oryzanol effects on oxidative stress factors in animal model of sepsis
2019Co-Authors: Elmira Zolali, Hadi HamishehkarAbstract:Evaluation of Gamma Oryzanol effects on oxidative stress factors in animal model of sepsis , Evaluation of Gamma Oryzanol effects on oxidative stress factors in animal model of sepsis , بانک اطلاعاتی جامع اطلاع رسانی پایان نامه های کشور
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effects of Gamma Oryzanol on factors of oxidative stress and sepsis induced lung injury in experimental animal model
Iranian Journal of Basic Medical Sciences, 2015Co-Authors: Elmira Zolali, Hadi Hamishehkar, Parina Asgharian, Maryam Kouhsoltani, Hajhir KhodaiiAbstract:Objective (s): There is corroborating evidence to substantiate redox imbalance and oxidative stress in sepsis that finally leads to organ damage or even death. Gamma Oryzanol (GO) is one of the major bioactive components in rice bran has been considered to function as an antioxidant. The present study was carried out to evaluate the antioxidant activity of Gamma Oryzanol in vitro and its efficacy in sepsis. Materials and Methods: To induce sepsis, cecal ligation and puncture (CLP) method was performed on the rats. A study group of forty male Wistar rats were divided into the following groups: sham group; CLP group; 50 mg/kg GO- treated CLP group and 100 mg/kg GO- treated CLP group. GO was administered with an oral gavage 2 hr prior to inducing sepsis. Tissue and blood samples were collected 12 hr after CLP to prepare tissue sections for histopathological study and assay the oxidative stress biomarkers including: SOD (Superoxide Dismutase), TAC (total antioxidant capacity), MDA (Malondialdehyde), MPO (Myeloperoxidase) and PAI-1 (Plasminogen Activator Inhibitor-1). Data are given as mean ± SD. The ANOVA with Tukey post hoc test was used to determine the differences between groups and P Results: TAC level increased in GO- treated CLP groups (P<0.05). Inflammation score of lung tissue and MPO activity were significantly lower in GO treated CLP group (P<0.05). Conclusion:It seems that GO has a protective effect on lung inflammation and improves the body redox capacity during sepsis.
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Effects of Gamma Oryzanol on factors of oxidative stress and sepsis-induced lung injury in experimental animal model
Iranian journal of basic medical sciences, 2015Co-Authors: Elmira Zolali, Hadi Hamishehkar, Parina Asgharian, Maryam Kouhsoltani, Hajhir KhodaiiAbstract:Objective (s): There is corroborating evidence to substantiate redox imbalance and oxidative stress in sepsis that finally leads to organ damage or even death. Gamma Oryzanol (GO) is one of the major bioactive components in rice bran has been considered to function as an antioxidant. The present study was carried out to evaluate the antioxidant activity of Gamma Oryzanol in vitro and its efficacy in sepsis. Materials and Methods: To induce sepsis, cecal ligation and puncture (CLP) method was performed on the rats. A study group of forty male Wistar rats were divided into the following groups: sham group; CLP group; 50 mg/kg GO- treated CLP group and 100 mg/kg GO- treated CLP group. GO was administered with an oral gavage 2 hr prior to inducing sepsis. Tissue and blood samples were collected 12 hr after CLP to prepare tissue sections for histopathological study and assay the oxidative stress biomarkers including: SOD (Superoxide Dismutase), TAC (total antioxidant capacity), MDA (Malondialdehyde), MPO (Myeloperoxidase) and PAI-1 (Plasminogen Activator Inhibitor-1). Data are given as mean ± SD. The ANOVA with Tukey post hoc test was used to determine the differences between groups and P Results: TAC level increased in GO- treated CLP groups (P
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Effects of Gamma Oryzanol on oxidative stress factors in animal model of sepsis
2014Co-Authors: Elmira Zolali, Hadi Hamishehkar, Nasrin Maleki-dizajiAbstract:There is corroborating evidence to substantiate redox imbalance and oxidative stress in sepsis that finally leads to organ damage or even death. Gamma Oryzanol (GO) is one of the major bioactive components in rice bran has been considered to function as an antioxidant. To investigate the antioxidative effects of GO on oxidative stress factors in septic animals. To induce sepsis, cecal ligation and puncture (CLP) method was performed on the rats. A study group of forty male Wistar rats were divided into the following groups: sham group; CLP group; 50 mg/kg GO- treated CLP group and 100 mg/kg GO- treated CLP group. GO was administered with an oral gavage 2 hour prior to inducing sepsis. Blood and tissue samples were collected 12 hours after CLP to assay the oxidative stress biomarkers and prepare tissue sections for histopathological study. Whole blood total antioxidant capacity (TAC) level increased in GO- treated CLP groups. Inflammation score of lung tissue and Myeloperoxidase (MPO) activity were significantly lower in GO treated CLP group. Glutathione peroxidise (GPx) level of lung tissue was improved by treatment with GO. It seems that GO has a protective effect on lung inflammation and improves the body redox capacity during sepsis.
