The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Lotta Gustafsson - One of the best experts on this subject based on the ideXlab platform.
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comparison of bacteria and fungus binding mesh foam and Gauze as fillers in negative pressure wound therapy pressure transduction wound edge contraction microvascular blood flow and fluid retention
International Wound Journal, 2013Co-Authors: Malin Malmsjö, Richard Ingemansson, Sandra Lindstedt, Lotta GustafssonAbstract:Bacteria- and fungus-binding mesh binds with and inactivates bacteria and fungus, which makes it an interesting alternative, wound filler for negative pressure wound therapy (NPWT). This study was conducted to compare the performance of pathogen-binding mesh, foam and Gauze as wound fillers in NPWT with regard to pressure transduction, fluid retention, wound contraction and microvascular blood flow. Wounds on the backs of 16 pigs were filled with pathogen-binding mesh, foam or Gauze and treated with NPWT. The immediate effects of 0, -40, -60, -80 and -120 mmHg, on pressure transduction and blood flow were examined in eight pigs using laser Doppler velocimetry. Wound contraction and fluid retention were studied during 72 hours of NPWT at -80 and -120 mmHg in the other eight pigs. Pathogen-binding mesh, Gauze and foam provide similar pressure transduction to the wound bed during NPWT. Blood flow was found to decrease 0.5 cm laterally from the wound edge and increase 2.5 cm from the wound edge, but was unaltered 5.0 cm from the wound edge. The increase in blood flow was similar with all wound fillers. The decrease in blood flow was more pronounced with foam than with Gauze and pathogen-binding mesh. Similarly, wound contraction was more pronounced with foam, than with Gauze and pathogen-binding mesh. Wound fluid retention was the same in foam and pathogen-binding mesh, while more fluid was retained in the wound when using Gauze. The blood flow 0.5-5 cm from the wound edge and the contraction of the wound during NPWT were similar when using pathogen-binding mesh and Gauze. Wound fluid was efficiently removed when using pathogen-binding mesh, which may explain previous findings that granulation tissue formation is more rapid under pathogen-binding mesh than under Gauze. This, in combination with its pathogen-binding properties, makes this mesh an interesting wound filler for use in NPWT.
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comparison of bacteria and fungus binding mesh foam and Gauze as fillers in negative pressure wound therapy pressure transduction wound edge contraction microvascular blood flow and fluid retention
International Wound Journal, 2013Co-Authors: Malin Malmsjö, Richard Ingemansson, Sandra Lindstedt, Lotta GustafssonAbstract:Bacteria- and fungus-binding mesh binds and inactivates bacteria and fungus, which makes it interesting, alternative, wound filler for negative pressure wound therapy (NPWT). This study was conducted to compare the performance of pathogen-binding mesh, foam and Gauze as wound fillers in NPWT with regard to pressure transduction, fluid retention, wound contraction and microvascular blood flow. Wounds on the backs of 16 pigs were filled with pathogen-binding mesh, foam or Gauze and treated with NPWT. The immediate effects of 0, -40, -60, -80 and -120 mmHg, on pressure transduction and blood flow were examined in eight pigs using laser Doppler velocimetry. Wound contraction and fluid retention were studied during 72 hours of NPWT at -80 and -120 mmHg in the other eight pigs. Pathogen-binding mesh, Gauze and foam provide similar pressure transduction to the wound bed during NPWT. Blood flow was found to decrease 0·5 cm laterally from the wound edge and increase 2·5 cm from the wound edge, but was unaltered 5·0 cm from the wound edge. The increase in blood flow was similar with all wound fillers. The decrease in blood flow was more pronounced with foam than with Gauze and pathogen-binding mesh. Similarly, wound contraction was more pronounced with foam, than with Gauze and pathogen-binding mesh. Wound fluid retention was the same in foam and pathogen-binding mesh, while more fluid was retained in the wound when using Gauze. The blood flow 0·5-5 cm from the wound edge and the contraction of the wound during NPWT were similar when using pathogen-binding mesh and Gauze. Wound fluid was efficiently removed through the pathogen-binding mesh, which may explain previous findings that granulation tissue formation is more rapid under pathogen-binding mesh than under Gauze. This, in combination with its pathogen-binding properties, makes this mesh an interesting wound filler for use in NPWT. (Less)
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negative pressure wound therapy associated tissue trauma and pain a controlled in vivo study comparing foam and Gauze dressing removal by immunohistochemistry for substance p and calcitonin gene related peptide in the wound edge
Ostomy Wound Management, 2011Co-Authors: Malin Malmsjö, Lotta Gustafsson, Sandra Lindstedt Ingemansson, Richard IngemanssonAbstract:Pain upon negative pressure wound therapy (NPWT) dressing removal has been reported and is believed to be associ- ated with the observation that granulation tissue grows into foam. Wound tissue damage upon removal of the foam may cause the reported pain. Calcitonin gene-related peptide (CGRP) and substance P are neuropeptides that cause inflam- mation and signal pain and are known to be released when tissue trauma occurs. The aim of this controlled in vivo study was to compare the expression of CGRP and substance P in the wound bed in control wounds and following NPWT and foam or Gauze dressing removal. Eight pigs with two wounds each were treated with open-pore structure polyurethane foam or AMD Gauze and NPWT of 0 (control) or -80 mm Hg for 72 hours. Following removal of the wound filler, the ex- pression of CGRP and substance P was measured, using arbitrary units, in sections of biopsies from the wound bed using immunofluorescence techniques. Substance P and CGRP were more abundant in the wound edge following the removal of foam than of Gauze dressings and least abundant in control wounds. The immunofluorescence staining of the wound edge for CGRP was 52 ± 3 au after the removal of Gauze and 97 ± 5 au after the removal of foam (P <0.001). For substance P, the staining was 55 ± 3 au after Gauze removal and 95 ± 4 au after foam removal (P <0.001). CGRP and substance P staining was primarily located to nerves and leukocytes. The increase in CGRP and substance P immuno- fluorescence was especially prominent in the dermis but also was seen in subcutaneous and muscle tissue. Using Gauze may be one way of reducing NPWT dressing change-related pain. New wound fillers designed to optimize granulation tissue formation and minimize pain issues presumably will be developed in the near future. (Less)
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micro and macromechanical effects on the wound bed of negative pressure wound therapy using Gauze and foam
Annals of Plastic Surgery, 2010Co-Authors: Olga Borgquist, Richard Ingemansson, Lotta Gustafsson, Malin MalmsjöAbstract:Negative pressure wound therapy (NPWT) results in 2 types of tissue deformation, macrodeformation (ie, wound contraction) and microdeformation (ie, the interaction of tissue and dressing on a microscopic level). These effects have been delineated for one type of wound filler, foam, but not for Gauze. The mechanical deformation initiates a signaling cascade which ultimately leads to wound healing. The aim of the present study was to examine the effect of Gauze and foam on macro- and microdeformation during treatment with negative pressure. An in vivo porcine peripheral wound model was used. NPWT was applied for 72 hours at 0, -75, and -125 mm Hg, using either foam or Gauze as wound filler. The mechanical effects of NPWT were examined by measuring the wound surface area reduction and by histologic analysis of the wound bed tissue. Similar degrees of wound contraction (macrodeformation) were seen during NPWT regardless if foam or Gauze was used. After negative pressure had been discontinued, the wound stayed contracted. There was no difference in wound contraction between -75 and -125 mm Hg. Biopsies of the wound bed revealed a repeating pattern of wound surface undulations and small tissue blebs ("tissue mushrooms") were pulled into the pores of the foam dressing and the spaces between the threads in the Gauze dressing (microdeformation). This pattern was obvious in wounds treated both with foam and Gauze, at atmospheric pressure (0 mm Hg) as well as at subatmospheric pressures (-75 and -125 mm Hg). The degrees of micro- and macrodeformation of the wound bed are similar after NPWT regardless if foam or Gauze is used as wound filler.
Malin Malmsjö - One of the best experts on this subject based on the ideXlab platform.
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comparison of bacteria and fungus binding mesh foam and Gauze as fillers in negative pressure wound therapy pressure transduction wound edge contraction microvascular blood flow and fluid retention
International Wound Journal, 2013Co-Authors: Malin Malmsjö, Richard Ingemansson, Sandra Lindstedt, Lotta GustafssonAbstract:Bacteria- and fungus-binding mesh binds with and inactivates bacteria and fungus, which makes it an interesting alternative, wound filler for negative pressure wound therapy (NPWT). This study was conducted to compare the performance of pathogen-binding mesh, foam and Gauze as wound fillers in NPWT with regard to pressure transduction, fluid retention, wound contraction and microvascular blood flow. Wounds on the backs of 16 pigs were filled with pathogen-binding mesh, foam or Gauze and treated with NPWT. The immediate effects of 0, -40, -60, -80 and -120 mmHg, on pressure transduction and blood flow were examined in eight pigs using laser Doppler velocimetry. Wound contraction and fluid retention were studied during 72 hours of NPWT at -80 and -120 mmHg in the other eight pigs. Pathogen-binding mesh, Gauze and foam provide similar pressure transduction to the wound bed during NPWT. Blood flow was found to decrease 0.5 cm laterally from the wound edge and increase 2.5 cm from the wound edge, but was unaltered 5.0 cm from the wound edge. The increase in blood flow was similar with all wound fillers. The decrease in blood flow was more pronounced with foam than with Gauze and pathogen-binding mesh. Similarly, wound contraction was more pronounced with foam, than with Gauze and pathogen-binding mesh. Wound fluid retention was the same in foam and pathogen-binding mesh, while more fluid was retained in the wound when using Gauze. The blood flow 0.5-5 cm from the wound edge and the contraction of the wound during NPWT were similar when using pathogen-binding mesh and Gauze. Wound fluid was efficiently removed when using pathogen-binding mesh, which may explain previous findings that granulation tissue formation is more rapid under pathogen-binding mesh than under Gauze. This, in combination with its pathogen-binding properties, makes this mesh an interesting wound filler for use in NPWT.
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comparison of bacteria and fungus binding mesh foam and Gauze as fillers in negative pressure wound therapy pressure transduction wound edge contraction microvascular blood flow and fluid retention
International Wound Journal, 2013Co-Authors: Malin Malmsjö, Richard Ingemansson, Sandra Lindstedt, Lotta GustafssonAbstract:Bacteria- and fungus-binding mesh binds and inactivates bacteria and fungus, which makes it interesting, alternative, wound filler for negative pressure wound therapy (NPWT). This study was conducted to compare the performance of pathogen-binding mesh, foam and Gauze as wound fillers in NPWT with regard to pressure transduction, fluid retention, wound contraction and microvascular blood flow. Wounds on the backs of 16 pigs were filled with pathogen-binding mesh, foam or Gauze and treated with NPWT. The immediate effects of 0, -40, -60, -80 and -120 mmHg, on pressure transduction and blood flow were examined in eight pigs using laser Doppler velocimetry. Wound contraction and fluid retention were studied during 72 hours of NPWT at -80 and -120 mmHg in the other eight pigs. Pathogen-binding mesh, Gauze and foam provide similar pressure transduction to the wound bed during NPWT. Blood flow was found to decrease 0·5 cm laterally from the wound edge and increase 2·5 cm from the wound edge, but was unaltered 5·0 cm from the wound edge. The increase in blood flow was similar with all wound fillers. The decrease in blood flow was more pronounced with foam than with Gauze and pathogen-binding mesh. Similarly, wound contraction was more pronounced with foam, than with Gauze and pathogen-binding mesh. Wound fluid retention was the same in foam and pathogen-binding mesh, while more fluid was retained in the wound when using Gauze. The blood flow 0·5-5 cm from the wound edge and the contraction of the wound during NPWT were similar when using pathogen-binding mesh and Gauze. Wound fluid was efficiently removed through the pathogen-binding mesh, which may explain previous findings that granulation tissue formation is more rapid under pathogen-binding mesh than under Gauze. This, in combination with its pathogen-binding properties, makes this mesh an interesting wound filler for use in NPWT. (Less)
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negative pressure wound therapy associated tissue trauma and pain a controlled in vivo study comparing foam and Gauze dressing removal by immunohistochemistry for substance p and calcitonin gene related peptide in the wound edge
Ostomy Wound Management, 2011Co-Authors: Malin Malmsjö, Lotta Gustafsson, Sandra Lindstedt Ingemansson, Richard IngemanssonAbstract:Pain upon negative pressure wound therapy (NPWT) dressing removal has been reported and is believed to be associ- ated with the observation that granulation tissue grows into foam. Wound tissue damage upon removal of the foam may cause the reported pain. Calcitonin gene-related peptide (CGRP) and substance P are neuropeptides that cause inflam- mation and signal pain and are known to be released when tissue trauma occurs. The aim of this controlled in vivo study was to compare the expression of CGRP and substance P in the wound bed in control wounds and following NPWT and foam or Gauze dressing removal. Eight pigs with two wounds each were treated with open-pore structure polyurethane foam or AMD Gauze and NPWT of 0 (control) or -80 mm Hg for 72 hours. Following removal of the wound filler, the ex- pression of CGRP and substance P was measured, using arbitrary units, in sections of biopsies from the wound bed using immunofluorescence techniques. Substance P and CGRP were more abundant in the wound edge following the removal of foam than of Gauze dressings and least abundant in control wounds. The immunofluorescence staining of the wound edge for CGRP was 52 ± 3 au after the removal of Gauze and 97 ± 5 au after the removal of foam (P <0.001). For substance P, the staining was 55 ± 3 au after Gauze removal and 95 ± 4 au after foam removal (P <0.001). CGRP and substance P staining was primarily located to nerves and leukocytes. The increase in CGRP and substance P immuno- fluorescence was especially prominent in the dermis but also was seen in subcutaneous and muscle tissue. Using Gauze may be one way of reducing NPWT dressing change-related pain. New wound fillers designed to optimize granulation tissue formation and minimize pain issues presumably will be developed in the near future. (Less)
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effects of foam or Gauze on sternum wound contraction distension and heart and lung damage during negative pressure wound therapy of porcine sternotomy wounds
Interactive Cardiovascular and Thoracic Surgery, 2011Co-Authors: Malin Malmsjö, Sandra Lindstedt, Richard IngemanssonAbstract:The study was performed to compare the effects of negative-pressure wound therapy (NPWT) using Gauze and foam on wound edge movement and the macroscopic appearance of the heart and lungs after NPWT. Sternotomy wounds were created in 6 kg pigs. Negative pressures of -40, -70, -120 and -160 mmHg were applied and the following were evaluated: wound contraction, distension and the macroscopic appearance of the heart and lungs after NPWT. Wound contraction was greater when using foam than Gauze (3.5±0.3 cm and 1.3±0.2 cm, respectively, P<0.01). The application of traction to the lateral edges of the sternotomy resulted in greater wound distention with foam than with Gauze (5.3±0.3 cm and 3.6±0.2 cm, respectively, P<0.001). After using foam, the surface of the heart was red and mottled, and lung emphysema and sometimes, lung rupture were observed. After using Gauze, the organ surface had no markings. The study shows that foam allows greater wound contraction and distension than Gauze. This movement of the wound edges may cause damage to the underlying organs. There is less damage to the heart and lungs when using Gauze than foam. Keywords: Animal model; Wound contraction; Experimental surgery; Heart rupture; Negative-pressure wound therapy; Sternotomy wound. (Less)
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micro and macromechanical effects on the wound bed of negative pressure wound therapy using Gauze and foam
Annals of Plastic Surgery, 2010Co-Authors: Olga Borgquist, Richard Ingemansson, Lotta Gustafsson, Malin MalmsjöAbstract:Negative pressure wound therapy (NPWT) results in 2 types of tissue deformation, macrodeformation (ie, wound contraction) and microdeformation (ie, the interaction of tissue and dressing on a microscopic level). These effects have been delineated for one type of wound filler, foam, but not for Gauze. The mechanical deformation initiates a signaling cascade which ultimately leads to wound healing. The aim of the present study was to examine the effect of Gauze and foam on macro- and microdeformation during treatment with negative pressure. An in vivo porcine peripheral wound model was used. NPWT was applied for 72 hours at 0, -75, and -125 mm Hg, using either foam or Gauze as wound filler. The mechanical effects of NPWT were examined by measuring the wound surface area reduction and by histologic analysis of the wound bed tissue. Similar degrees of wound contraction (macrodeformation) were seen during NPWT regardless if foam or Gauze was used. After negative pressure had been discontinued, the wound stayed contracted. There was no difference in wound contraction between -75 and -125 mm Hg. Biopsies of the wound bed revealed a repeating pattern of wound surface undulations and small tissue blebs ("tissue mushrooms") were pulled into the pores of the foam dressing and the spaces between the threads in the Gauze dressing (microdeformation). This pattern was obvious in wounds treated both with foam and Gauze, at atmospheric pressure (0 mm Hg) as well as at subatmospheric pressures (-75 and -125 mm Hg). The degrees of micro- and macrodeformation of the wound bed are similar after NPWT regardless if foam or Gauze is used as wound filler.
Yu Dong Huang - One of the best experts on this subject based on the ideXlab platform.
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hemostatic effect of aminated multiwalled carbon nanotubes oxidized regenerated cellulose nanocomposites
Journal of Nanoscience and Nanotechnology, 2019Co-Authors: Baode Zhang, Jin Mei He, Yu Dong Huang, Ali Nabipour Chakoli, Andrey N. AleshinAbstract:: We have investigated the covalent conjugation of aminated multiwalled carbon nanotubes (MWCNTNH₂)s with Oxidized Regenerated Cellulose (ORC) in order to enhance the hemostatic effect. The MWCNT-NH₂s were prepared by functionalization of pristine MWCNTs (pMWCNTs) using amine groups. Neat ORC Gauze and MWCNT-NH₂s were reacted using glutamic acid as cross linking bridge. We investigated an amination of pMWCNTs as well as the dispersion of MWCNT-NH₂s in the ORC Gauze as matrix and their interfacial interactions by SEM and FT-IR. The results revealed that relatively strong interaction exists between aminated MWCNTs and the ORC macromolecules. The hydrophilicity test results in the significant increment of water uptake of MWCNT-NH₂s/ORC composites with increasing the concentration of MWCNT-NH₂s in composite. The in-vitro procoagulation test shows that the MWCNT-NH₂s/ORC Gauzes have significant procoagulant activity. The hemostatic evaluation of MWCNT-NH₂s/ORC composites on rabbits shows that the aminated MWCNTs increase the rate of blood stopping and hence they decrease the blood loosing from injured sites. Hemostatic evaluation indicates that the MWCNT-NH₂s/ORC Gauze has a valuable hemostatic performance. The products of platelets release reaction, activated platelets glycoprotein and activated clotting enzymes were increased simultaneously. The mechanism of the hemostasis for MWCNT-NH₂s/ORC Gauze is discussed.
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biodegradable n o carboxymethyl chitosan oxidized regenerated cellulose composite Gauze as a barrier for preventing postoperative adhesion
Carbohydrate Polymers, 2019Co-Authors: Feng Cheng, Ya Dong Wu, Jin Mei He, Hongbin Li, Guangyu Wu, Yu Dong HuangAbstract:Abstract Tissue adhesion is one of the most common complications after surgery (especially after abdominal surgery), causing notable influences after the damaged tissue has healed. A physical barrier placed between the wound site and the adjacent tissues is a convenient and highly effective technique to minimize or prevent abdominal adhesions. In this work, the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite Gauze was prepared. The N, O-CS/ORC composite Gauze is degradable; in addition, the Gauze exhibits excellent antimicrobial functionality against S. aureus and E. coli bacteria. Moreover, the notable hemostatic efficacy of the N, O-CS/ORC composite Gauze was confirmed in rabbit livers/ears as models. The results showed that the N, O-CS/ORC composite Gauze is nontoxic toward normal cells and can restrain the adhesion of fibroblast cells, thereby indicating its potential use in preventing tissue adhesion. In addition, the rat models for abdominal defect-cecum abrasion were used to evaluate the efficacy of N, O-CS/ORC composite Gauze in preventing tissue adhesions after surgery. The results indicated that the N, O-CS/ORC composite Gauze can significantly prevent postsurgical peritoneal adhesions. Finally, the potential anti-adhesion mechanism of the N, O-CS/ORC composite Gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC, was investigated.
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carbon nanotube modified oxidized regenerated cellulose Gauzes for hemostatic applications
Carbohydrate Polymers, 2018Co-Authors: Feng Cheng, Jin Mei He, Yunfeng Wang, Yunjia Song, Hongbin Li, Yu Dong HuangAbstract:Abstract Functionalized carbon nanotubes have recently received interest because of their unique properties, especially in the biomedical field. In this research, unmodified multiwalled carbon nanotubes (MWCNTs), and functionalized carbon nanotubes with amino groups (MWCNTs-NH2) and carboxyl groups (MWCNTs-COOH) were grafted to oxidized regenerated cellulose (ORC) Gauze to fabricate novel functionalized ORC, and the performance of the functionalized Gauze was investigated. The functionalized ORC was characterized by FT-IR, XPS and SEM, which showed the different kinds of CNTs grafted on its surface. The XPS results demonstrated the successful incorporation of functionalized MWCNTs in the active layer of modified ORC Gauze. Meanwhile, the specific surface area of the CNTs modified functionalized ORC Gauze was improved in varying degrees, whereas the porosity was slightly decreased. Furthermore, hydrophilicity experiment results presented a significant increment in water uptake of the functionalized CNTs grafted to the surface of the ORC Gauze. Results of the hemostatic performance test on rabbit ear artery and liver showed that compared with the original ORC Gauze, the bleeding time was significantly reduced when using the functionalized CNTs modified ORC hemostatic Gauze. Moreover, the results also showed that the MWCNTs-COOH/ORC functionalized Gauze had outstanding hemostatic efficiency.
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antibacterial and hemostatic composite Gauze of n o carboxymethyl chitosan oxidized regenerated cellulose
RSC Advances, 2016Co-Authors: Feng Cheng, Jin Mei He, Jiwei Li, Yu Dong HuangAbstract:Viscose Gauze was oxidized with NO2/CCl4 to prepare Oxidized Regenerated Cellulose (ORC). Then, ORC was modified by the water-soluble chitosan derivative N,O-carboxymethyl chitosan (N,O-CS). This was synthesized via the reaction of chitosan with chloroacetic acid as the etherification agent in the presence of alkaline. It was prepared by introducing carboxymethyl groups onto the N and O-positions of the chitosan and the substituting degree (DS) of N,O-CS reached 1.68. Composite Gauzes with a 2–8 w/v% N,O-CS content were prepared in water solution. Composite Gauzes could still maintain their original morphological form and have excellent water-solubility. The formation of an amide bond between the carboxyl group of ORC and the amino group of N,O-CS was confirmed by FT-IR and elemental analysis. To study the influence of carboxymethyl groups on chitosan, the thermal stability and crystallinity of N,O-CS were tested by XRD and TG. Based on SEM images, N,O-CS tended to be adsorbed on the surface of ORC fiber. The antibacterial performance of N,O-CS/ORC Gauzes was enhanced with the increases of N,O-CS content. Moreover, N,O-CS/ORC Gauzes showed excellent bactericidal activity against both Gram-positive and Gram-negative bacteria. The hemostatic evaluation indicated that the N,O-CS/ORC composite Gauzes in rabbit livers had dramatic hemostatic efficacy. The prepared composite Gauzes were anticipated to be an optimal material in preventing post-operative adhesion.
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hemostatic antibacterial and degradable performance of the water soluble chitosan coated oxidized regenerated cellulose Gauze
Fibers and Polymers, 2014Co-Authors: Jin Mei He, Ya Dong Wu, Yu Dong Huang, Feng Wen Wang, Weilu Cheng, Bo FuAbstract:In this research, the oxidized regenerated cellulose (ORC) Gauze was prepared by oxidation of regenerated cellulose using NO2/CCl4, subsequently treated with the dissolved chitosan (CTS) in aqueous acetic acid (CTS/ORC) and finally neutralized with NaOH/C2H5OH (CTS/ORC-Na). The hemostatic, antibacterial and degradable properties of treated ORC-based Gauze were evaluated. The results of the hemostatic test on rabbit liver and ear-artery injuries showed that the attachment of high molecular weight chitosan to the surface of ORC Gauze significantly improved the hemostatic effect of ORC without compromising the antibacterial and degradability of ORC. It is found that the water-soluble CTS/ORC-Na Gauze was more suitable as the hemostatic material applied in rabbit liver injury in comparison with in ear-artery injury and it further improved the hemostatic efficacy and enhanced the degradable rate. It is promised that the CTS/ORC Gauze and the water-soluble CTS/ORC-Na Gauze would be applicable in medical fields such as surgical absorbable hemostats for control bleeding.
Jin Mei He - One of the best experts on this subject based on the ideXlab platform.
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hemostatic effect of aminated multiwalled carbon nanotubes oxidized regenerated cellulose nanocomposites
Journal of Nanoscience and Nanotechnology, 2019Co-Authors: Baode Zhang, Jin Mei He, Yu Dong Huang, Ali Nabipour Chakoli, Andrey N. AleshinAbstract:: We have investigated the covalent conjugation of aminated multiwalled carbon nanotubes (MWCNTNH₂)s with Oxidized Regenerated Cellulose (ORC) in order to enhance the hemostatic effect. The MWCNT-NH₂s were prepared by functionalization of pristine MWCNTs (pMWCNTs) using amine groups. Neat ORC Gauze and MWCNT-NH₂s were reacted using glutamic acid as cross linking bridge. We investigated an amination of pMWCNTs as well as the dispersion of MWCNT-NH₂s in the ORC Gauze as matrix and their interfacial interactions by SEM and FT-IR. The results revealed that relatively strong interaction exists between aminated MWCNTs and the ORC macromolecules. The hydrophilicity test results in the significant increment of water uptake of MWCNT-NH₂s/ORC composites with increasing the concentration of MWCNT-NH₂s in composite. The in-vitro procoagulation test shows that the MWCNT-NH₂s/ORC Gauzes have significant procoagulant activity. The hemostatic evaluation of MWCNT-NH₂s/ORC composites on rabbits shows that the aminated MWCNTs increase the rate of blood stopping and hence they decrease the blood loosing from injured sites. Hemostatic evaluation indicates that the MWCNT-NH₂s/ORC Gauze has a valuable hemostatic performance. The products of platelets release reaction, activated platelets glycoprotein and activated clotting enzymes were increased simultaneously. The mechanism of the hemostasis for MWCNT-NH₂s/ORC Gauze is discussed.
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biodegradable n o carboxymethyl chitosan oxidized regenerated cellulose composite Gauze as a barrier for preventing postoperative adhesion
Carbohydrate Polymers, 2019Co-Authors: Feng Cheng, Ya Dong Wu, Jin Mei He, Hongbin Li, Guangyu Wu, Yu Dong HuangAbstract:Abstract Tissue adhesion is one of the most common complications after surgery (especially after abdominal surgery), causing notable influences after the damaged tissue has healed. A physical barrier placed between the wound site and the adjacent tissues is a convenient and highly effective technique to minimize or prevent abdominal adhesions. In this work, the N, O-carboxymethyl chitosan/oxidized regenerated cellulose (N, O-CS/ORC) composite Gauze was prepared. The N, O-CS/ORC composite Gauze is degradable; in addition, the Gauze exhibits excellent antimicrobial functionality against S. aureus and E. coli bacteria. Moreover, the notable hemostatic efficacy of the N, O-CS/ORC composite Gauze was confirmed in rabbit livers/ears as models. The results showed that the N, O-CS/ORC composite Gauze is nontoxic toward normal cells and can restrain the adhesion of fibroblast cells, thereby indicating its potential use in preventing tissue adhesion. In addition, the rat models for abdominal defect-cecum abrasion were used to evaluate the efficacy of N, O-CS/ORC composite Gauze in preventing tissue adhesions after surgery. The results indicated that the N, O-CS/ORC composite Gauze can significantly prevent postsurgical peritoneal adhesions. Finally, the potential anti-adhesion mechanism of the N, O-CS/ORC composite Gauze, which may attribute to the combination of barrier function and instinct activity of N, O-CS and ORC, was investigated.
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carbon nanotube modified oxidized regenerated cellulose Gauzes for hemostatic applications
Carbohydrate Polymers, 2018Co-Authors: Feng Cheng, Jin Mei He, Yunfeng Wang, Yunjia Song, Hongbin Li, Yu Dong HuangAbstract:Abstract Functionalized carbon nanotubes have recently received interest because of their unique properties, especially in the biomedical field. In this research, unmodified multiwalled carbon nanotubes (MWCNTs), and functionalized carbon nanotubes with amino groups (MWCNTs-NH2) and carboxyl groups (MWCNTs-COOH) were grafted to oxidized regenerated cellulose (ORC) Gauze to fabricate novel functionalized ORC, and the performance of the functionalized Gauze was investigated. The functionalized ORC was characterized by FT-IR, XPS and SEM, which showed the different kinds of CNTs grafted on its surface. The XPS results demonstrated the successful incorporation of functionalized MWCNTs in the active layer of modified ORC Gauze. Meanwhile, the specific surface area of the CNTs modified functionalized ORC Gauze was improved in varying degrees, whereas the porosity was slightly decreased. Furthermore, hydrophilicity experiment results presented a significant increment in water uptake of the functionalized CNTs grafted to the surface of the ORC Gauze. Results of the hemostatic performance test on rabbit ear artery and liver showed that compared with the original ORC Gauze, the bleeding time was significantly reduced when using the functionalized CNTs modified ORC hemostatic Gauze. Moreover, the results also showed that the MWCNTs-COOH/ORC functionalized Gauze had outstanding hemostatic efficiency.
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antibacterial and hemostatic composite Gauze of n o carboxymethyl chitosan oxidized regenerated cellulose
RSC Advances, 2016Co-Authors: Feng Cheng, Jin Mei He, Jiwei Li, Yu Dong HuangAbstract:Viscose Gauze was oxidized with NO2/CCl4 to prepare Oxidized Regenerated Cellulose (ORC). Then, ORC was modified by the water-soluble chitosan derivative N,O-carboxymethyl chitosan (N,O-CS). This was synthesized via the reaction of chitosan with chloroacetic acid as the etherification agent in the presence of alkaline. It was prepared by introducing carboxymethyl groups onto the N and O-positions of the chitosan and the substituting degree (DS) of N,O-CS reached 1.68. Composite Gauzes with a 2–8 w/v% N,O-CS content were prepared in water solution. Composite Gauzes could still maintain their original morphological form and have excellent water-solubility. The formation of an amide bond between the carboxyl group of ORC and the amino group of N,O-CS was confirmed by FT-IR and elemental analysis. To study the influence of carboxymethyl groups on chitosan, the thermal stability and crystallinity of N,O-CS were tested by XRD and TG. Based on SEM images, N,O-CS tended to be adsorbed on the surface of ORC fiber. The antibacterial performance of N,O-CS/ORC Gauzes was enhanced with the increases of N,O-CS content. Moreover, N,O-CS/ORC Gauzes showed excellent bactericidal activity against both Gram-positive and Gram-negative bacteria. The hemostatic evaluation indicated that the N,O-CS/ORC composite Gauzes in rabbit livers had dramatic hemostatic efficacy. The prepared composite Gauzes were anticipated to be an optimal material in preventing post-operative adhesion.
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hemostatic antibacterial and degradable performance of the water soluble chitosan coated oxidized regenerated cellulose Gauze
Fibers and Polymers, 2014Co-Authors: Jin Mei He, Ya Dong Wu, Yu Dong Huang, Feng Wen Wang, Weilu Cheng, Bo FuAbstract:In this research, the oxidized regenerated cellulose (ORC) Gauze was prepared by oxidation of regenerated cellulose using NO2/CCl4, subsequently treated with the dissolved chitosan (CTS) in aqueous acetic acid (CTS/ORC) and finally neutralized with NaOH/C2H5OH (CTS/ORC-Na). The hemostatic, antibacterial and degradable properties of treated ORC-based Gauze were evaluated. The results of the hemostatic test on rabbit liver and ear-artery injuries showed that the attachment of high molecular weight chitosan to the surface of ORC Gauze significantly improved the hemostatic effect of ORC without compromising the antibacterial and degradability of ORC. It is found that the water-soluble CTS/ORC-Na Gauze was more suitable as the hemostatic material applied in rabbit liver injury in comparison with in ear-artery injury and it further improved the hemostatic efficacy and enhanced the degradable rate. It is promised that the CTS/ORC Gauze and the water-soluble CTS/ORC-Na Gauze would be applicable in medical fields such as surgical absorbable hemostats for control bleeding.
Richard Ingemansson - One of the best experts on this subject based on the ideXlab platform.
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comparison of bacteria and fungus binding mesh foam and Gauze as fillers in negative pressure wound therapy pressure transduction wound edge contraction microvascular blood flow and fluid retention
International Wound Journal, 2013Co-Authors: Malin Malmsjö, Richard Ingemansson, Sandra Lindstedt, Lotta GustafssonAbstract:Bacteria- and fungus-binding mesh binds with and inactivates bacteria and fungus, which makes it an interesting alternative, wound filler for negative pressure wound therapy (NPWT). This study was conducted to compare the performance of pathogen-binding mesh, foam and Gauze as wound fillers in NPWT with regard to pressure transduction, fluid retention, wound contraction and microvascular blood flow. Wounds on the backs of 16 pigs were filled with pathogen-binding mesh, foam or Gauze and treated with NPWT. The immediate effects of 0, -40, -60, -80 and -120 mmHg, on pressure transduction and blood flow were examined in eight pigs using laser Doppler velocimetry. Wound contraction and fluid retention were studied during 72 hours of NPWT at -80 and -120 mmHg in the other eight pigs. Pathogen-binding mesh, Gauze and foam provide similar pressure transduction to the wound bed during NPWT. Blood flow was found to decrease 0.5 cm laterally from the wound edge and increase 2.5 cm from the wound edge, but was unaltered 5.0 cm from the wound edge. The increase in blood flow was similar with all wound fillers. The decrease in blood flow was more pronounced with foam than with Gauze and pathogen-binding mesh. Similarly, wound contraction was more pronounced with foam, than with Gauze and pathogen-binding mesh. Wound fluid retention was the same in foam and pathogen-binding mesh, while more fluid was retained in the wound when using Gauze. The blood flow 0.5-5 cm from the wound edge and the contraction of the wound during NPWT were similar when using pathogen-binding mesh and Gauze. Wound fluid was efficiently removed when using pathogen-binding mesh, which may explain previous findings that granulation tissue formation is more rapid under pathogen-binding mesh than under Gauze. This, in combination with its pathogen-binding properties, makes this mesh an interesting wound filler for use in NPWT.
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comparison of bacteria and fungus binding mesh foam and Gauze as fillers in negative pressure wound therapy pressure transduction wound edge contraction microvascular blood flow and fluid retention
International Wound Journal, 2013Co-Authors: Malin Malmsjö, Richard Ingemansson, Sandra Lindstedt, Lotta GustafssonAbstract:Bacteria- and fungus-binding mesh binds and inactivates bacteria and fungus, which makes it interesting, alternative, wound filler for negative pressure wound therapy (NPWT). This study was conducted to compare the performance of pathogen-binding mesh, foam and Gauze as wound fillers in NPWT with regard to pressure transduction, fluid retention, wound contraction and microvascular blood flow. Wounds on the backs of 16 pigs were filled with pathogen-binding mesh, foam or Gauze and treated with NPWT. The immediate effects of 0, -40, -60, -80 and -120 mmHg, on pressure transduction and blood flow were examined in eight pigs using laser Doppler velocimetry. Wound contraction and fluid retention were studied during 72 hours of NPWT at -80 and -120 mmHg in the other eight pigs. Pathogen-binding mesh, Gauze and foam provide similar pressure transduction to the wound bed during NPWT. Blood flow was found to decrease 0·5 cm laterally from the wound edge and increase 2·5 cm from the wound edge, but was unaltered 5·0 cm from the wound edge. The increase in blood flow was similar with all wound fillers. The decrease in blood flow was more pronounced with foam than with Gauze and pathogen-binding mesh. Similarly, wound contraction was more pronounced with foam, than with Gauze and pathogen-binding mesh. Wound fluid retention was the same in foam and pathogen-binding mesh, while more fluid was retained in the wound when using Gauze. The blood flow 0·5-5 cm from the wound edge and the contraction of the wound during NPWT were similar when using pathogen-binding mesh and Gauze. Wound fluid was efficiently removed through the pathogen-binding mesh, which may explain previous findings that granulation tissue formation is more rapid under pathogen-binding mesh than under Gauze. This, in combination with its pathogen-binding properties, makes this mesh an interesting wound filler for use in NPWT. (Less)
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negative pressure wound therapy associated tissue trauma and pain a controlled in vivo study comparing foam and Gauze dressing removal by immunohistochemistry for substance p and calcitonin gene related peptide in the wound edge
Ostomy Wound Management, 2011Co-Authors: Malin Malmsjö, Lotta Gustafsson, Sandra Lindstedt Ingemansson, Richard IngemanssonAbstract:Pain upon negative pressure wound therapy (NPWT) dressing removal has been reported and is believed to be associ- ated with the observation that granulation tissue grows into foam. Wound tissue damage upon removal of the foam may cause the reported pain. Calcitonin gene-related peptide (CGRP) and substance P are neuropeptides that cause inflam- mation and signal pain and are known to be released when tissue trauma occurs. The aim of this controlled in vivo study was to compare the expression of CGRP and substance P in the wound bed in control wounds and following NPWT and foam or Gauze dressing removal. Eight pigs with two wounds each were treated with open-pore structure polyurethane foam or AMD Gauze and NPWT of 0 (control) or -80 mm Hg for 72 hours. Following removal of the wound filler, the ex- pression of CGRP and substance P was measured, using arbitrary units, in sections of biopsies from the wound bed using immunofluorescence techniques. Substance P and CGRP were more abundant in the wound edge following the removal of foam than of Gauze dressings and least abundant in control wounds. The immunofluorescence staining of the wound edge for CGRP was 52 ± 3 au after the removal of Gauze and 97 ± 5 au after the removal of foam (P <0.001). For substance P, the staining was 55 ± 3 au after Gauze removal and 95 ± 4 au after foam removal (P <0.001). CGRP and substance P staining was primarily located to nerves and leukocytes. The increase in CGRP and substance P immuno- fluorescence was especially prominent in the dermis but also was seen in subcutaneous and muscle tissue. Using Gauze may be one way of reducing NPWT dressing change-related pain. New wound fillers designed to optimize granulation tissue formation and minimize pain issues presumably will be developed in the near future. (Less)
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effects of foam or Gauze on sternum wound contraction distension and heart and lung damage during negative pressure wound therapy of porcine sternotomy wounds
Interactive Cardiovascular and Thoracic Surgery, 2011Co-Authors: Malin Malmsjö, Sandra Lindstedt, Richard IngemanssonAbstract:The study was performed to compare the effects of negative-pressure wound therapy (NPWT) using Gauze and foam on wound edge movement and the macroscopic appearance of the heart and lungs after NPWT. Sternotomy wounds were created in 6 kg pigs. Negative pressures of -40, -70, -120 and -160 mmHg were applied and the following were evaluated: wound contraction, distension and the macroscopic appearance of the heart and lungs after NPWT. Wound contraction was greater when using foam than Gauze (3.5±0.3 cm and 1.3±0.2 cm, respectively, P<0.01). The application of traction to the lateral edges of the sternotomy resulted in greater wound distention with foam than with Gauze (5.3±0.3 cm and 3.6±0.2 cm, respectively, P<0.001). After using foam, the surface of the heart was red and mottled, and lung emphysema and sometimes, lung rupture were observed. After using Gauze, the organ surface had no markings. The study shows that foam allows greater wound contraction and distension than Gauze. This movement of the wound edges may cause damage to the underlying organs. There is less damage to the heart and lungs when using Gauze than foam. Keywords: Animal model; Wound contraction; Experimental surgery; Heart rupture; Negative-pressure wound therapy; Sternotomy wound. (Less)
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micro and macromechanical effects on the wound bed of negative pressure wound therapy using Gauze and foam
Annals of Plastic Surgery, 2010Co-Authors: Olga Borgquist, Richard Ingemansson, Lotta Gustafsson, Malin MalmsjöAbstract:Negative pressure wound therapy (NPWT) results in 2 types of tissue deformation, macrodeformation (ie, wound contraction) and microdeformation (ie, the interaction of tissue and dressing on a microscopic level). These effects have been delineated for one type of wound filler, foam, but not for Gauze. The mechanical deformation initiates a signaling cascade which ultimately leads to wound healing. The aim of the present study was to examine the effect of Gauze and foam on macro- and microdeformation during treatment with negative pressure. An in vivo porcine peripheral wound model was used. NPWT was applied for 72 hours at 0, -75, and -125 mm Hg, using either foam or Gauze as wound filler. The mechanical effects of NPWT were examined by measuring the wound surface area reduction and by histologic analysis of the wound bed tissue. Similar degrees of wound contraction (macrodeformation) were seen during NPWT regardless if foam or Gauze was used. After negative pressure had been discontinued, the wound stayed contracted. There was no difference in wound contraction between -75 and -125 mm Hg. Biopsies of the wound bed revealed a repeating pattern of wound surface undulations and small tissue blebs ("tissue mushrooms") were pulled into the pores of the foam dressing and the spaces between the threads in the Gauze dressing (microdeformation). This pattern was obvious in wounds treated both with foam and Gauze, at atmospheric pressure (0 mm Hg) as well as at subatmospheric pressures (-75 and -125 mm Hg). The degrees of micro- and macrodeformation of the wound bed are similar after NPWT regardless if foam or Gauze is used as wound filler.
