The Experts below are selected from a list of 24390 Experts worldwide ranked by ideXlab platform

Shulamit B. Wallach-dayan - One of the best experts on this subject based on the ideXlab platform.

  • Bleomycin-Treated Chimeric Thy1-Deficient Mice with Thy1-Deficient Myofibroblasts and Thy-Positive Lymphocytes Resolve Inflammation without Affecting the Fibrotic Response
    Mediators of inflammation, 2015
    Co-Authors: Pazit Y. Cohen, Raphael Breuer, Philip Zisman, Shulamit B. Wallach-dayan
    Abstract:

    Lung fibrosis is characterized by abnormal accumulation of fibroblasts in the interstitium of the alveolar space. Two populations of myofibroblasts, distinguished by Thy1 expression, are detected in human and murine lungs. Accumulation of Thy1-negative (Thy1−) myofibroblasts was shown in the lungs of humans with idiopathic pulmonary fibrosis (IPF) and of bleomycin-treated mice. We aimed to identify Genetic changes in lung myofibroblasts following Thy1 crosslinking and assess the impact of specific lung myofibroblast Thy1-deficiency, in vivo, in bleomycin-injured mouse lungs. Thy1 increased in mouse lung lymphocytes following bleomycin injury but decreased in myofibroblasts when fibrosis was at the highest point (14 days), as assessed by immunohistochemistry. Using Gene Chip Analysis, we detected that myofibroblast Thy1 crosslinking mediates downregulation of Genes promoting cell proliferation, survival, and differentiation, and reduces production of extracellular matrix (ECM) components, while concurrently mediating the upregulation of Genes known to foster inflammation and immunological functions. Chimeric Thy1-deficient mice with Thy1+ lymphocytes and Thy1− myofibroblasts showed fibrosis similar to wild-type mice and an increased number of CD4/CD25 regulatory T cells, with a concomitant decrease in inflammation. Lung myofibroblasts downregulate Thy1 expression to increase their proliferation but to diminish the in vivo inflammatory milieu. Inflammation is not essential for evolution of fibrosis as was previously stated.

Charles N Serhan - One of the best experts on this subject based on the ideXlab platform.

  • leukotriene b4 and lipoxin a4 are regulatory signals for neural stem cell proliferation and differentiation
    The FASEB Journal, 2006
    Co-Authors: Koichiro Wada, Makoto Arita, Atsushi Nakajima, Kazufumi Katayama, Chiho Kudo, Yoshinori Kamisaki, Charles N Serhan
    Abstract:

    Leukotrienes (LTs) and lipoxins (LXs) are lipid mediators that play a key role in regulating acute inflammatory responses. Their roles in neural stem cell (NSC) functions are of interest. We showed here that LTB4 and LXA4 regulated proliferation and differentiation of murine NSCs that were isolated from embryo brains. Proliferation of NSCs was stimulated by LTB4 (3 to 100 nM) and blocked by receptor antagonist (IC50=2.7 μM). In contrast, LXA4, and its aspirin-triggered-15-epi-LXA4 stable analog attenuated growth of NSCs at as little as 1 nM. Both lipoxygenase (LOX) inhibitors and LTB4 receptor antagonists caused apoptosis and cell death. Gene Chip Analysis revealed that growth-related Gene expressions such as epidermal growth factor (EGF) receptor, cyclin E, p27, and caspase 8 were tightly regulated by LTB4; LXA4 gave the opposite Gene expressions. In addition to proliferation, LTB4 induced differentiation of NSCs into neurons as monitored by neurite outgrowth and MAP2 expression. These results indicate f...

  • leukotriene b4 and lipoxin a4 are regulatory signals for neural stem cell proliferation and differentiation
    The FASEB Journal, 2006
    Co-Authors: Koichiro Wada, Makoto Arita, Atsushi Nakajima, Kazufumi Katayama, Chiho Kudo, Yoshinori Kamisaki, Charles N Serhan
    Abstract:

    Leukotrienes (LTs) and lipoxins (LXs) are lipid mediators that play a key role in regulating acute inflammatory responses. Their roles in neural stem cell (NSC) functions are of interest. We showed here that LTB(4) and LXA(4) regulated proliferation and differentiation of murine NSCs that were isolated from embryo brains. Proliferation of NSCs was stimulated by LTB(4) (3 to 100 nM) and blocked by receptor antagonist (IC(50)=2.7 microM). In contrast, LXA(4), and its aspirin-triggered-15-epi-LXA(4) stable analog attenuated growth of NSCs at as little as 1 nM. Both lipoxygenase (LOX) inhibitors and LTB(4) receptor antagonists caused apoptosis and cell death. Gene Chip Analysis revealed that growth-related Gene expressions such as epidermal growth factor (EGF) receptor, cyclin E, p27, and caspase 8 were tightly regulated by LTB(4); LXA(4) gave the opposite Gene expressions. In addition to proliferation, LTB(4) induced differentiation of NSCs into neurons as monitored by neurite outgrowth and MAP2 expression. These results indicate for the first time that LTB(4) and LXA(4) directly regulate proliferation and differentiation of NSCs, suggesting these new pathways may be useful in restoring stem cells.

Zhanjing Huang - One of the best experts on this subject based on the ideXlab platform.

  • The wheat Gene TaST can increase the salt tolerance of transgenic Arabidopsis
    Plant Cell Reports, 2011
    Co-Authors: Xi Huang, Yinzhu Shen, Gang Wang, Zhanjing Huang
    Abstract:

    On the basis of the results of Gene Chip Analysis of the salt-tolerant wheat mutant RH8706-49 under conditions of salt stress, we identified and cloned an unknown salt-induced Gene TaST (Triticum aestivum salt-tolerant). Real-time quantitative PCR Analysis showed that the expression of the Gene was induced by salt stress. Transgenic Arabidopsis plants overexpressing the TaST Gene showed higher salt tolerance than the wild-type controls. Subcellular localization studies revealed that the protein encoded by this Gene was in the nucleus. In comparison with wild-type controls, transgenic Arabidopsis plants accumulated more Ca2+, soluble sugar, and proline and less Na+ under salt stress. Real-time quantitative PCR Analysis showed that Arabidopsis plants overexpressing TaST also showed increased expression of many stress-related Genes. All these findings indicated that TaST can enhance the salt tolerance of transgenic Arabidopsis plants.

Ryuji Ueno - One of the best experts on this subject based on the ideXlab platform.

  • s1766 cobiprostone protects against indomethacin damage through activation of clc 2 a Gene Chip Analysis and clc 2 ablation study
    Gastroenterology, 2009
    Co-Authors: John Cuppoletti, Danuta H. Malinowska, Jayati Chakrabarti, Ryuji Ueno
    Abstract:

    G A A b st ra ct s used as control cells. These cells were treated with various concentrations of hydrogen peroxide (0-0.14mM). The cell viability was determined by MTT assay and the direct plasma membrane damage was analyzed by lactate dehydrogenase (LDH)-release assay. Results: Cell viability after hydrogen peroxide treatment was significantly higher in HSP70-over expressing cells compared with that in control cells (IEC-6-mock cells)(P<0.0001). Hydrogen peroxide induced cell damage to IEC-6-mock cells in a concentration-dependent manner. On the other hand, cell damage was significantly suppressed in HSP70-highly expressing cells based on LDH-release (P < 0.0002) Conclusion: These results presented in this study strongly indicate that molecular chaperone (HSP70) plays an important role in protecting small intestinal epithelial cells against hydrogen peroxide-induced cell injury In Vitro, and raised the possibility of protection of small intestinal epithelial cells by manipulation of HSP70 expression. In the therapeutic point of view, it is suggested here that chaperone-inducing therapy could be an important role in new strategy for small intestinal mucosal injury including inflammatory bowel diseases and NSAID-induced mucosal injury.

  • S1766 Cobiprostone Protects Against Indomethacin Damage Through Activation of Clc-2: A Gene Chip Analysis and Clc-2 Ablation Study
    Gastroenterology, 2009
    Co-Authors: John Cuppoletti, Danuta H. Malinowska, Jayati Chakrabarti, Ryuji Ueno
    Abstract:

    G A A b st ra ct s used as control cells. These cells were treated with various concentrations of hydrogen peroxide (0-0.14mM). The cell viability was determined by MTT assay and the direct plasma membrane damage was analyzed by lactate dehydrogenase (LDH)-release assay. Results: Cell viability after hydrogen peroxide treatment was significantly higher in HSP70-over expressing cells compared with that in control cells (IEC-6-mock cells)(P

Xuetao Pei - One of the best experts on this subject based on the ideXlab platform.

  • Stem cell engineering: the new Generation of cellular therapeutics.
    International Journal of Hematology, 2002
    Co-Authors: Xuetao Pei
    Abstract:

    Hematopoietic stem cells (HSCs) supply all blood cells throughout life by making use of their self-renewal and multilineage differentiation capabilities. Over the last few years, transplantation of hematopoietic stem and progenitor cell from mobilized blood stem cells, umbilical cord blood and selected CD34+ cells has been used for treatment of patients with hematologic and non-hematologic malignances. The techniques have become available that allow the extensive proliferation, orderly differentiation, functional activation and Gene transfer of hematopoietic stem/progenitor cells in ex vivo culture systems. These techniques have now developed to the point at which clinical trials are now underway in a variety of settings for the applications of hematopoietic stem cell transplantation, hematopoietic support after high-dose chemotherapy, immunotherapy of cancers, and Gene therapy. The article will discuss the characteristics, detecting assays, surface markers, expansion, orderly differentiation, cell therapy and Gene therapy of hematopoietic stem/progenitor cells. Except that, it will also discuss some advanced achievements of stem cell research, such as the multilineage differentiation of marrow-derived mesenchymal stem cells. Some results of different Gene expression between stem cells and induced cells by Gene Chip Analysis will be reported.