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Jae Youn Cho - One of the best experts on this subject based on the ideXlab platform.
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correlation between angiotensin converting enzyme ace inhibitor induced dry cough and ace Gene Insertion deletion i d polymorphism
Tuberculosis and Respiratory Diseases, 1999Co-Authors: Je Hyeong Kim, Kyung Kyu Kim, Hye Cheol Jeong, Sung Yong Lee, Young Hwan Kwon, So Ra Lee, Sang Youb Lee, Sin Hyung Lee, Dae Ryong Cha, Jae Youn ChoAbstract:Background: Persistent nonproductive cough is a major adverse effect encountered with ACE inhibitor treatment and the most frequent reason for withdrawal of the drug. The mechanism of cough was postulated to be associated with accumulation of bronchial irritants which are substrates of ACE. It has been speculated that occurrence of this adverse effect is Genetically predetermined ; in particular, variants of the Genes encoding ACE. To investigate this relationship, we determined ACE Gene Insertion/Deletion polymorphism in subjects with and without a history of ACE inhibitor-induced cough. Methods: Among the 339 patients with ACE inhibitor treatment, subjects who developed cough that resolved when not taking medication were designated to cough group and other subjects who did not complain cough were designated to non-cough group. Clinical characteristics of the patients were collected by review of medical records. ACE genotypes were determined by PCR amplification of DNA from peripheral blood and agarose gel electrophoresis. Results: 37 patients complained of dry cough(cough group) and 302 patients did not complained of cough(non-cough group). The incidence of ACE inhibitor induced dry cough was 10.9%. There was a preponderance of females in the cough group (M : F=24.3% : 75.7%) compared to the non-cough group (M : F=49.7% : 50.3%, p=0.004). There was no significant difference in mean age, underlying diseases, and kinds and frequencies of ACE inhibitors and their mean dosage between the both groups. ACE genotypic frequencies were I/I : I/D : D/D=16.2% : 18.9% : 64.9% in the cough group and 18.9% : 18.2% : 62.9% in the non-cough group which showed no significant difference between the both groups(p=0.926). Allelic frequencies were I : D = 25.7% : 74.3% and 28.0% : 72.0% in the cough and non-cough group respectively and the difference was not significant(p = 0.676). Conclusion: The incidence of ACE inhibitor-induced cough are 10.9%, and women are more susceptible to ACE inhibitor-induced cough. ACE inhibitor-induced dry cough is not associated with ACE Gene Insertion/Deletion polymorphism.
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Correlation Between Angiotensin-Converting Enzyme(ACE) Inhibitor Induced Dry Cough and ACE Gene Insertion/Deletion(I/D) Polymorphism
Tuberculosis and Respiratory Diseases, 1999Co-Authors: Je Hyeong Kim, Kyung Kyu Kim, Hye Cheol Jeong, Sung Yong Lee, Young Hwan Kwon, So Ra Lee, Sang Youb Lee, Sin Hyung Lee, Dae Ryong Cha, Jae Youn ChoAbstract:Background: Persistent nonproductive cough is a major adverse effect encountered with ACE inhibitor treatment and the most frequent reason for withdrawal of the drug. The mechanism of cough was postulated to be associated with accumulation of bronchial irritants which are substrates of ACE. It has been speculated that occurrence of this adverse effect is Genetically predetermined ; in particular, variants of the Genes encoding ACE. To investigate this relationship, we determined ACE Gene Insertion/Deletion polymorphism in subjects with and without a history of ACE inhibitor-induced cough. Methods: Among the 339 patients with ACE inhibitor treatment, subjects who developed cough that resolved when not taking medication were designated to cough group and other subjects who did not complain cough were designated to non-cough group. Clinical characteristics of the patients were collected by review of medical records. ACE genotypes were determined by PCR amplification of DNA from peripheral blood and agarose gel electrophoresis. Results: 37 patients complained of dry cough(cough group) and 302 patients did not complained of cough(non-cough group). The incidence of ACE inhibitor induced dry cough was 10.9%. There was a preponderance of females in the cough group (M : F=24.3% : 75.7%) compared to the non-cough group (M : F=49.7% : 50.3%, p=0.004). There was no significant difference in mean age, underlying diseases, and kinds and frequencies of ACE inhibitors and their mean dosage between the both groups. ACE genotypic frequencies were I/I : I/D : D/D=16.2% : 18.9% : 64.9% in the cough group and 18.9% : 18.2% : 62.9% in the non-cough group which showed no significant difference between the both groups(p=0.926). Allelic frequencies were I : D = 25.7% : 74.3% and 28.0% : 72.0% in the cough and non-cough group respectively and the difference was not significant(p = 0.676). Conclusion: The incidence of ACE inhibitor-induced cough are 10.9%, and women are more susceptible to ACE inhibitor-induced cough. ACE inhibitor-induced dry cough is not associated with ACE Gene Insertion/Deletion polymorphism.
Taymour Mostafa - One of the best experts on this subject based on the ideXlab platform.
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ace Gene Insertion deletion polymorphism seminal associations in infertile men
The Journal of Urology, 2012Co-Authors: Adel Zalata, Heba K Morsy, Abd Elnaser Badawy, Samir Elhanbly, Taymour MostafaAbstract:Purpose: We assessed seminal associations of the ACE* Gene Insertion/deletion polymorphism in infertile men.Materials and Methods: A total of 405 men were investigated, divided into healthy fertile men, and those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. They underwent semen analysis, and assessment of sperm acrosin activity, hypo-osmotic swelling, seminal 8-iso-prostaglandin-F2α, total antioxidant capacity, α-glucosidase and ACE Gene polymorphisms.Result: The ACE* Insertion/Insertion genotype was noted in 182 men, including 76.5% of healthy fertile men, and 47.4%, 39.8% and 17.6% of those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. The ACE* Insertion/deletion genotype was noted in 133 men, including 13.7% of healthy fertile men, and 42.3%, 27.5% and 47.2% of those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. The ACE* deletion/deletion genotype was id...
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ACE Gene Insertion/Deletion Polymorphism Seminal Associations in Infertile Men
The Journal of Urology, 2012Co-Authors: Adel Zalata, Heba K Morsy, Abd Elnaser Badawy, Samir Elhanbly, Taymour MostafaAbstract:Purpose: We assessed seminal associations of the ACE* Gene Insertion/deletion polymorphism in infertile men.Materials and Methods: A total of 405 men were investigated, divided into healthy fertile men, and those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. They underwent semen analysis, and assessment of sperm acrosin activity, hypo-osmotic swelling, seminal 8-iso-prostaglandin-F2α, total antioxidant capacity, α-glucosidase and ACE Gene polymorphisms.Result: The ACE* Insertion/Insertion genotype was noted in 182 men, including 76.5% of healthy fertile men, and 47.4%, 39.8% and 17.6% of those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. The ACE* Insertion/deletion genotype was noted in 133 men, including 13.7% of healthy fertile men, and 42.3%, 27.5% and 47.2% of those with asthenozoospermia, asthenoteratozoospermia and oligoasthenoteratozoospermia, respectively. The ACE* deletion/deletion genotype was id...
Je Hyeong Kim - One of the best experts on this subject based on the ideXlab platform.
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correlation between angiotensin converting enzyme ace inhibitor induced dry cough and ace Gene Insertion deletion i d polymorphism
Tuberculosis and Respiratory Diseases, 1999Co-Authors: Je Hyeong Kim, Kyung Kyu Kim, Hye Cheol Jeong, Sung Yong Lee, Young Hwan Kwon, So Ra Lee, Sang Youb Lee, Sin Hyung Lee, Dae Ryong Cha, Jae Youn ChoAbstract:Background: Persistent nonproductive cough is a major adverse effect encountered with ACE inhibitor treatment and the most frequent reason for withdrawal of the drug. The mechanism of cough was postulated to be associated with accumulation of bronchial irritants which are substrates of ACE. It has been speculated that occurrence of this adverse effect is Genetically predetermined ; in particular, variants of the Genes encoding ACE. To investigate this relationship, we determined ACE Gene Insertion/Deletion polymorphism in subjects with and without a history of ACE inhibitor-induced cough. Methods: Among the 339 patients with ACE inhibitor treatment, subjects who developed cough that resolved when not taking medication were designated to cough group and other subjects who did not complain cough were designated to non-cough group. Clinical characteristics of the patients were collected by review of medical records. ACE genotypes were determined by PCR amplification of DNA from peripheral blood and agarose gel electrophoresis. Results: 37 patients complained of dry cough(cough group) and 302 patients did not complained of cough(non-cough group). The incidence of ACE inhibitor induced dry cough was 10.9%. There was a preponderance of females in the cough group (M : F=24.3% : 75.7%) compared to the non-cough group (M : F=49.7% : 50.3%, p=0.004). There was no significant difference in mean age, underlying diseases, and kinds and frequencies of ACE inhibitors and their mean dosage between the both groups. ACE genotypic frequencies were I/I : I/D : D/D=16.2% : 18.9% : 64.9% in the cough group and 18.9% : 18.2% : 62.9% in the non-cough group which showed no significant difference between the both groups(p=0.926). Allelic frequencies were I : D = 25.7% : 74.3% and 28.0% : 72.0% in the cough and non-cough group respectively and the difference was not significant(p = 0.676). Conclusion: The incidence of ACE inhibitor-induced cough are 10.9%, and women are more susceptible to ACE inhibitor-induced cough. ACE inhibitor-induced dry cough is not associated with ACE Gene Insertion/Deletion polymorphism.
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Correlation Between Angiotensin-Converting Enzyme(ACE) Inhibitor Induced Dry Cough and ACE Gene Insertion/Deletion(I/D) Polymorphism
Tuberculosis and Respiratory Diseases, 1999Co-Authors: Je Hyeong Kim, Kyung Kyu Kim, Hye Cheol Jeong, Sung Yong Lee, Young Hwan Kwon, So Ra Lee, Sang Youb Lee, Sin Hyung Lee, Dae Ryong Cha, Jae Youn ChoAbstract:Background: Persistent nonproductive cough is a major adverse effect encountered with ACE inhibitor treatment and the most frequent reason for withdrawal of the drug. The mechanism of cough was postulated to be associated with accumulation of bronchial irritants which are substrates of ACE. It has been speculated that occurrence of this adverse effect is Genetically predetermined ; in particular, variants of the Genes encoding ACE. To investigate this relationship, we determined ACE Gene Insertion/Deletion polymorphism in subjects with and without a history of ACE inhibitor-induced cough. Methods: Among the 339 patients with ACE inhibitor treatment, subjects who developed cough that resolved when not taking medication were designated to cough group and other subjects who did not complain cough were designated to non-cough group. Clinical characteristics of the patients were collected by review of medical records. ACE genotypes were determined by PCR amplification of DNA from peripheral blood and agarose gel electrophoresis. Results: 37 patients complained of dry cough(cough group) and 302 patients did not complained of cough(non-cough group). The incidence of ACE inhibitor induced dry cough was 10.9%. There was a preponderance of females in the cough group (M : F=24.3% : 75.7%) compared to the non-cough group (M : F=49.7% : 50.3%, p=0.004). There was no significant difference in mean age, underlying diseases, and kinds and frequencies of ACE inhibitors and their mean dosage between the both groups. ACE genotypic frequencies were I/I : I/D : D/D=16.2% : 18.9% : 64.9% in the cough group and 18.9% : 18.2% : 62.9% in the non-cough group which showed no significant difference between the both groups(p=0.926). Allelic frequencies were I : D = 25.7% : 74.3% and 28.0% : 72.0% in the cough and non-cough group respectively and the difference was not significant(p = 0.676). Conclusion: The incidence of ACE inhibitor-induced cough are 10.9%, and women are more susceptible to ACE inhibitor-induced cough. ACE inhibitor-induced dry cough is not associated with ACE Gene Insertion/Deletion polymorphism.
Shahid M Khan - One of the best experts on this subject based on the ideXlab platform.
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a novel Gene Insertion marker out gimo method for transGene expression and Gene complementation in rodent malaria parasites
PLOS ONE, 2011Co-Authors: Takeshi Annoura, Mohammed Sajid, Severine Chevalleymaurel, Jai Ramesar, Onny Klop, Blandine Frankefayard, Chris J Janse, Shahid M KhanAbstract:Research on the biology of malaria parasites has greatly benefited from the application of reverse Genetic technologies, in particular through the analysis of Gene deletion mutants and studies on transgenic parasites that express heterologous or mutated proteins. However, transfection in Plasmodium is limited by the paucity of drug-selectable markers that hampers subsequent Genetic modification of the same mutant. We report the development of a novel ‘Gene Insertion/marker out’ (GIMO) method for two rodent malaria parasites, which uses negative selection to rapidly Generate transgenic mutants ready for subsequent modifications. We have created reference mother lines for both P. berghei ANKA and P. yoelii 17XNL that serve as recipient parasites for GIMO-transfection. Compared to existing protocols GIMO-transfection greatly simplifies and speeds up the Generation of mutants expressing heterologous proteins, free of drug-resistance Genes, and requires far fewer laboratory animals. In addition we demonstrate that GIMO-transfection is also a simple and fast method for Genetic complementation of mutants with a Gene deletion or mutation. The implementation of GIMO-transfection procedures should greatly enhance Plasmodium reverse-Genetic research.
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A Novel ‘Gene Insertion/Marker Out’ (GIMO) Method for TransGene Expression and Gene Complementation in Rodent Malaria Parasites
PLOS ONE, 2011Co-Authors: Takeshi Annoura, Mohammed Sajid, Jai Ramesar, Onny Klop, Chris J Janse, Séverine Chevalley-maurel, Blandine Franke-fayard, Shahid M KhanAbstract:Research on the biology of malaria parasites has greatly benefited from the application of reverse Genetic technologies, in particular through the analysis of Gene deletion mutants and studies on transgenic parasites that express heterologous or mutated proteins. However, transfection in Plasmodium is limited by the paucity of drug-selectable markers that hampers subsequent Genetic modification of the same mutant. We report the development of a novel ‘Gene Insertion/marker out’ (GIMO) method for two rodent malaria parasites, which uses negative selection to rapidly Generate transgenic mutants ready for subsequent modifications. We have created reference mother lines for both P. berghei ANKA and P. yoelii 17XNL that serve as recipient parasites for GIMO-transfection. Compared to existing protocols GIMO-transfection greatly simplifies and speeds up the Generation of mutants expressing heterologous proteins, free of drug-resistance Genes, and requires far fewer laboratory animals. In addition we demonstrate that GIMO-transfection is also a simple and fast method for Genetic complementation of mutants with a Gene deletion or mutation. The implementation of GIMO-transfection procedures should greatly enhance Plasmodium reverse-Genetic research.
Ruying Hu - One of the best experts on this subject based on the ideXlab platform.
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associations of ace Gene Insertion deletion polymorphism ace activity and ace mrna expression with hypertension in a chinese population
PLOS ONE, 2013Co-Authors: Qingfang He, Min Yu, Gina Wallar, Zuofeng Zhang, Lixin Wang, Xinwei Zhang, Ruying HuAbstract:Background The present study was designed to explore the association of angiotensin converting enzyme (ACE) Gene Insertion/deletion (I/D, rs4646994) polymorphism, plasma ACE activity, and circulating ACE mRNA expression with essential hypertension (EH) in a Chinese population. In addition, a new detection method for circulating ACE mRNA expression was explored. Methods The research was approved by the ethics committee of Zhejiang Provincial Center for Disease Prevention and Control. Written informed consent was obtained prior to the investigation. 221 hypertensives (cases) and 221 normotensives (controls) were interviewed, subjected to a physical examination, and provided blood for biochemical and Genetic tests. The ACE mRNA expression was analyzed by real time fluorescent quantitative Reverse Transcription PCR (FQ-RT-PCR). We performed logistic regression to assess associations of ACE I/D genotypes, ACE activity, and ACE mRNA expression levels with hypertension. Results The results of the multivariate logistic regression analysis showed that the additive model (ID, DD versus II) of the ACE genotype revealed an association with hypertension with adjusted OR of 1.43(95% CI: 1.04-1.97), and ACE ID genotype with adjusted OR of 1.72(95% CI: 1.01-2.92), DD genotype with adjusted OR of 1.94(95% CI: 1.01-3.73), respectively. In addition, our data also indicate that plasma ACE activity (adjusted OR was 1.13(95% CI: 1.08-1.18)) was significantly related to hypertension. However, the plasma ACE mRNA expressions were not different between the cases and controls. Conclusion ACE I/D polymorphism and ACE activity revealed significant influence on hypertension, while circulating ACE mRNA expression was not important factors associated with hypertension in this Chinese population. The detection of circulating ACE mRNA expression by FQ-RT-PCR might be a useful method for early screening and monitoring of EH.