The Experts below are selected from a list of 1857 Experts worldwide ranked by ideXlab platform
Jianmin Wan - One of the best experts on this subject based on the ideXlab platform.
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fine mapping of brown planthopper nilaparvata lugens stal resistance Gene bph28 t in rice oryza sativa l
2014Co-Authors: Yuqiang Liu, Yanling Liu, Ling Jiang, Linlong Liu, Chunming Wang, Xianian Cheng, Jianmin WanAbstract:The brown planthopper (BPH; Nilaparvata lugens Stal) is one of the most destructive insect pests of rice (Oryza sativa L.) throughout the Asian rice-growing countries. DV85 is a BPH-resistant indica variety. A single dominance Gene conferring resistance in DV85 was previously mapped on the long arm of chromosome 11. The objectives of this study were to investigate feeding behaviors of BPH on DV85 plants and fine-map the BPH resistance Gene, here designated Bph28(t). A seedling bulk test was conducted to identify resistant plant reactionsvg to BPH feeding. The results showed that the resistance of DV85 functions by means of tolerance during BPH attack, rather than non-preference and antibiosis. For fine mapping, two F2 populations were developed by crossing DV85 with the susceptible japonica variety Kinmaze and indica 9311. A high-resolution Genetic map harboring Bph28(t) was constructed and Bph28(t) was finally physically defined to an interval of 64.8 kb between markers Indel55 and Indel66. The fine-mapped Bph28(t) Gene will facilitate marker-assisted Gene Pyramiding for BPH resistance.
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qtl mapping of grain length in rice oryza sativa l using chromosome segment substitution lines
2006Co-Authors: Jiankang Wang, Xiangyuan Wan, Jose Crossa, Jonathan H Crouch, Jianfeng Weng, Huqu Zhai, Jianmin WanAbstract:Summary Chromosome segment substitution (CSS) lines have the potential for use in QTL fine mapping and map-based cloning. The standard t-test used in the idealized case that each CSS line has a single segment from the donor parent is not suitable for non-idealized CSS lines carrying several substituted segments from the donor parent. In this study, we present a likelihood ratio test based on stepwise regression (RSTEP-LRT) that can be used for QTL mapping in a population consisting of non-idealized CSS lines. Stepwise regression is used to select the most important segments for the trait of interest, and the likelihood ratio test is used to calculate the LOD score of each chromosome segment. This method is statistically equivalent to the standard t-test with idealized CSS lines. To further improve the power of QTL mapping, a method is proposed to decrease multicollinearity among markers (or chromosome segments). QTL mapping with an example CSS population in rice consisting of 65 non-idealized CSS lines and 82 chromosome segments indicated that a total of 18 segments on eight of the 12 rice chromosomes harboured QTLs affecting grain length under the LOD threshold of 2 . 5. Three major stable QTLs were detected in all eight environments. Some minor QTLs were not detected in all environments, but they could increase or decrease the grain length constantly. These minor Genes are also useful in marker-assisted Gene Pyramiding.
Qinghua Pan - One of the best experts on this subject based on the ideXlab platform.
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Genetic analysis and fine mapping of a rice brown planthopper nilaparvata lugens stal resistance Gene bph19 t
2006Co-Authors: J W Chen, Ling Wang, X F Pang, Qinghua PanAbstract:Genetic analysis and fine mapping of a resistance Gene against brown planthopper (BPH) biotype 2 in rice was performed using two F2 populations derived from two crosses between a resistant indica cultivar (cv.), AS20-1, and two susceptible japonica cvs., Aichi Asahi and Lijiangxintuanheigu. Insect resistance was evaluated using F1 plants and the two F2 populations. The results showed that a single recessive Gene, tentatively designated as bph19(t), conditioned the resistance in AS20-1. A linkage analysis, mainly employing microsatellite markers, was carried out in the two F2 populations through bulked segregant analysis and recessive class analysis (RCA), in combination with bioinformatics analysis (BIA). The resistance Gene locus bph19(t) was finely mapped to a region of about 1.0 cM on the short arm of chromosome 3, flanked by markers RM6308 and RM3134, where one known marker RM1022, and four new markers, b1, b2, b3 and b4, developed in the present study were co-segregating with the locus. To physically map this locus, the bph19(t)-linked markers were landed on bacterial artificial chromosome or P1 artificial chromosome clones of the reference cv., Nipponbare, released by the International Rice Genome Sequencing Project. Sequence information of these clones was used to construct a physical map of the bph19(t) locus, in silico, by BIA. The bph19(t) locus was physically defined to an interval of about 60 kb. The detailed Genetic and physical maps of the bph19(t) locus will facilitate marker-assisted Gene Pyramiding and cloning.
Samuel Chibuike Chukwu - One of the best experts on this subject based on the ideXlab platform.
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a review on Gene Pyramiding of agronomic biotic and abiotic traits in rice variety development
2019Co-Authors: Ibrahim Silas Akos, Shairul Izan Ramlee, Mohd Rafii Yusop, Mohd Razi Ismail, Norazyah Abd Aziz Shamsudin, Asfaliza Ramli, Bello Sani Haliru, Muhammad Ismaila, Samuel Chibuike ChukwuAbstract:Rice Oryza sativa L is a staple food crop, and its seeds are the most important component part of the agronomic trait of the cereal crop, rich in nutrient and of economic value to human and even livestock. But, it is often threatened by various abiotic and biotic conditions that reduce the yield, because of high incidences of infectious disease agents and non-pathogenic conditions respectively. Pyramiding of the requisite resistance and tolerance Genes into single elite high yielding variety of rice, confers wider spectrum of stress management, resulting to development of single multiline variety of rice. Marker-assisted selection utilizes DNA marker-linked primers for blast resistant Gene (RM8225; Piz , RM6836; Piz , Pi2 , Pi9 ), bacteria leaf blight (RM224; Xa-4 , RM122; xa-5 , RG136; xa-13 , RM21; Xa-21 ) and drought tolerance (RM236; qDTY 2.2 , RM520; qDTY 3.1 , RM511; qDTY 12.1 ) in pedigree, backcross and recurrent selection breeding methods. The objectives are to create awareness on the environmental safety of host-resistance, significance of single multiline resistance variety, effect of the interaction of stress conditions and associated simple sequence repeat (SSR) linked markers.
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marker assisted selection and Gene Pyramiding for resistance to bacterial leaf blight disease of rice oryza sativa l
2019Co-Authors: Samuel Chibuike Chukwu, Mohd Y. Rafii, Shairul Izan Ramlee, Siti Izera Ismail, Yussuf Oladosu, Emmanuel Okporie, Godwin Onyishi, Emeka Utobo, Lynda EkwuAbstract:AbstractMarker-assisted selection and Gene Pyramiding are very important breeding strategies for conferring broad spectrum and durable resistance against diseases causing yield loss in rice. One su...
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Marker-assisted selection and Gene Pyramiding for resistance to bacterial leaf blight disease of rice (Oryza sativa L.)
2019Co-Authors: Samuel Chibuike Chukwu, Mohd Y. Rafii, Shairul Izan Ramlee, Siti Izera Ismail, Yussuf Oladosu, Emmanuel Okporie, Godwin Onyishi, Emeka Utobo, Lynda Ekwu, Senesie SwarayAbstract:Marker-assisted selection and Gene Pyramiding are very important breeding strategies for conferring broad spectrum and durable resistance against diseases causing yield loss in rice. One such disease causing major set backs in rice production is bacterial leaf blight (BLB) caused by the pathogen Xanthomonas oryzae pv. oryzae. Molecular markers are very essential in both marker-assisted selection and Pyramiding of Genes, hence, many molecular marker techniques have already been developed. Presently, the most commonly used ones are DNA-based markers also known as molecular markers. The molecular markers are classified into two major categories based on the techniques used for detecting them. These are hybridization and polymerase chain reaction-based markers. Other types of markers available include the morphological (traditionally based) and biochemical (enzyme-based) markers. Host plant/varietal resistance is the most suitable means for controlling BLB disease of rice. Marker-assisted Gene Pyramiding has the potential to accelerate the breeding programmes and guarantee the durability of resistance conferred in the host plant. Therefore, this paper uncovers the utilization, economic importance, limitations and future prospects of marker-assisted selection and Gene Pyramiding for resistance to BLB disease of rice
Javaid Akhter Bhat - One of the best experts on this subject based on the ideXlab platform.
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marker assisted Gene Pyramiding magp for semi dwarfed bacterial blight resistance Genes into traditional basmati variety ranbir basmati
2021Co-Authors: R K Salgotra, Meenakshi Raina, Rajeev Rathore, Javaid Akhter BhatAbstract:Abstract Evolution of new virulent races has led to increased sensitiveness of the traditional basmati varieties to bacterial blight (BB) disease. Among the traditional basmati varieties, Ranbir Basmati is tall statured variety, and are susceptible to BB diseases. The variety has thin stem and prone to lodging leading to significant yield losses as well as deteriorate the grain qualities. In the present study, the marker-assisted backcross breeding (MABB) strategy were used for simultaneous stepwise transfer of two major BB resistance Genes (Xa21 and xa13) along with a dwarfing Gene (sd1) into Ranbir Basmati. The molecular markers viz., pTA 248, xa13prom and h linked to Xa21, xa13 and sd1, respectively were used in MABB programme. Both foreground and background selection approaches are used for Pyramiding of Genes of interest into Ranbir Basmati. The crosses were attempted for Pyramiding the BB resistance Genes along with dwarfing Gene from Punjab Basmati 4 (Xa21, xa13 and sd1) into Ranbir Basmati. MABB programme was carried out up to BC2F5 Generations. In total five semi-dwarf pyramided lines namely, SBTIL25, SBTIL39, SBTIL80, SBTIL121 and SBTIL31 have been identified for further field screening. These pyramided lines possess superior length/width ratio, excellent cooking properties and pleasant aroma. These five lines have been further evaluated for morpho-physiological and quality traits, and can be serve as potential donors for different rice pre-breeding programmes. These promising pyramided lines in bulk will be subjected to testing in multi-location and replicated trial for yield and disease resistance for their possible release as variety.
Yuqiang Liu - One of the best experts on this subject based on the ideXlab platform.
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fine mapping of brown planthopper nilaparvata lugens stal resistance Gene bph28 t in rice oryza sativa l
2014Co-Authors: Yuqiang Liu, Yanling Liu, Ling Jiang, Linlong Liu, Chunming Wang, Xianian Cheng, Jianmin WanAbstract:The brown planthopper (BPH; Nilaparvata lugens Stal) is one of the most destructive insect pests of rice (Oryza sativa L.) throughout the Asian rice-growing countries. DV85 is a BPH-resistant indica variety. A single dominance Gene conferring resistance in DV85 was previously mapped on the long arm of chromosome 11. The objectives of this study were to investigate feeding behaviors of BPH on DV85 plants and fine-map the BPH resistance Gene, here designated Bph28(t). A seedling bulk test was conducted to identify resistant plant reactionsvg to BPH feeding. The results showed that the resistance of DV85 functions by means of tolerance during BPH attack, rather than non-preference and antibiosis. For fine mapping, two F2 populations were developed by crossing DV85 with the susceptible japonica variety Kinmaze and indica 9311. A high-resolution Genetic map harboring Bph28(t) was constructed and Bph28(t) was finally physically defined to an interval of 64.8 kb between markers Indel55 and Indel66. The fine-mapped Bph28(t) Gene will facilitate marker-assisted Gene Pyramiding for BPH resistance.