The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform

L A Kirshenbaum - One of the best experts on this subject based on the ideXlab platform.

  • Adenovirus mediated — Gene Transfer into cardiomyocytes
    Molecular and Cellular Biochemistry, 1997
    Co-Authors: L A Kirshenbaum
    Abstract:

    To circumvent limitations imposed by conventional Gene Transfer techniques into cardiac muscle cells, we studied whether replication defective adenovirus would obviate this limitation to basic studies of signal transduction and transcriptional control processes in the heart. We demonstrate here the utility of adenovirus mediated Gene Transfer to introduce foreign DNA into post-mitotic terminally differentiated ventricular myocytes with uniformity and high efficiency. We also provide evidence for the Genetic modification of neonatal ventricular myocytes by adenovirus early region 1 (E1) proteins and their impact on cardiac Gene transcription and DNA synthesis respectively. Thus, for studies of transcriptional control processes in the heart, which until now have been restricted to neonatal ventricular myocytes; adenovirus mediated Gene Transfer provides a means to Genetically manipulate adult cardiac muscle cells. The advent of adenovirus Gene Transfer will extend our understanding of the molecular mechanisms that mediate basic cardiac disease and may ultimately provide a means to therapeutically mitigate the disease process. (Mol Cell Biochem 172: 13–21, 1997)

  • Adenovirus mediated-Gene Transfer into cardiomyocytes.
    Molecular and cellular biochemistry, 1997
    Co-Authors: L A Kirshenbaum
    Abstract:

    To circumvent limitations imposed by conventional Gene Transfer techniques into cardiac muscle cells, we studied whether replication defective adenovirus would obviate this limitation to basic studies of signal transduction and transcriptional control processes in the heart. We demonstrate here the utility of adenovirus mediated Gene Transfer to introduce foreign DNA into post-mitotic terminally differentiated ventricular myocytes with uniformity and high efficiency. We also provide evidence for the Genetic modification of neonatal ventricular myocytes by adenovirus early region 1 (E1) proteins and their impact on cardiac Gene transcription and DNA synthesis respectively. Thus, for studies of transcriptional control processes in the heart, which until now have been restricted to neonatal ventricular myocytes; adenovirus mediated Gene Transfer provides a means to Genetically manipulate adult cardiac muscle cells. The advent of adenovirus Gene Transfer will extend our understanding of the molecular mechanisms that mediate basic cardiac disease and may ultimately provide a means to therapeutically mitigate the disease process.

Timothy M. Crombleholme - One of the best experts on this subject based on the ideXlab platform.

  • Gene Transfer Techniques for Therapeutic AngioGenesis
    2020
    Co-Authors: Sundeep G. Keswani, Timothy M. Crombleholme
    Abstract:

    Background: The goal of therapeutic angioGenesis is to improve neovascularization in impaired tissue with the expectation that it will produce improved cellular, nutrient, and oxygen delivery resulting in enhanced wound healing. Gene Transfer of proangiogenic growth factors to the wound has been demonstrated to achieve this goal. The Problem: There are numerous candidate transGenes and several Gene delivery systems that have been examined. In this article, we review the most significant candidate transGenes, and the advantages and disadvantages of the currently available Gene Transfer techniques. Basic/Clinical Science Advances: Neovascularization is a complex process that is regulated by families of angiogenic growth factors, the most potent one being vascular endothelial growth factor. Vascular endothelial growth factor is primarily responsible for new vessel formation. Recently, new targets of angioGenesis have been elucidated. Stromal cell‐derived factor-1, endothelial nitric oxide synthase, and the upstream transcription factor, hypoxia inducible factor-1 alpha, form a complex system that controls the wound’s response to injury and represent new candidate vulnerary transGenes for therapeutic angioGenesis. To deliver these transGenes, there are both viral and nonviral Gene Transfer systems that can be used for in vivo or ex vivo Gene Transfer. Clinical Care Relevance: Effective and efficient growth factor‐induced neovascularization delivered by Gene Transfer techniques would represent a significant advance in the care of patients with chronic nonhealing wounds. Conclusion: Gene Transfer techniques of proangiogenic growth factors can positively influence the wound’s neovascularization and wound repair response to tissue injury. The ideal candidate vulnerary transGene and Gene delivery techniques are in the process of being determined.

  • Adenoviral-mediated Gene Transfer in wound healing
    Wound Repair and Regeneration, 2000
    Co-Authors: Timothy M. Crombleholme
    Abstract:

    The application of Gene Transfer strategies to wound healing is not an obvious use of this technology until one considers the important role of cytokines and growth factors in the normal wound healing response. Several Gene Transfer strategies have been proposed, from in vitro retroviral-mediated Gene Transfer with autologous transplantation, to in vivo plasmid based Gene Transfer as retroviral Gene Transfer. The limitations of these approaches have been efficiency of Gene Transfer, transGene expression and biologic response. Adenoviral-mediated Gene Transfer in wound healing is a relatively new application of this vector. The advantage of the adenovirus as a Gene Transfer vector lies in its ability to transduce nondividing cells of all types at very high efficiency without integration into the host cell's genome. The disadvantage of adenovirus as a vector is the relatively short duration of transGene expression and the inflammatory response it elicits. In the setting of wound healing brief duration of high levels of transGene may be all that is necessary to favorably influence wound healing. Secondly, as wound healing is fundamentally an inflammatory response, the inflammation elicited by the adenovirus may not be detrimental as long as the transGene is a growth factor with significant vulnerary effects such as platelet-derived growth factor-B. This review summarizes the current state of adenoviral-mediated Gene Transfer in experimental models of impaired wound healing which have laid the groundwork for proposed phase I clinical trials of adenoviral-mediated Gene Transfer of platelet-derived growth factor-B in chronic venous leg ulcers and chronic nonhealing diabetic foot ulcers. Adenoviral-mediated Gene Transfer is a useful tool in the study of the role of specific cytokines and growth factors in normal and impaired wound healing. Adenoviral-mediated Gene Transfer may hold significant promise for clinical application as a means of efficient growth factor delivery in correcting impaired wound healing.

H. Chao - One of the best experts on this subject based on the ideXlab platform.

  • Parvovirus-mediated Gene Transfer for the haemophilias.
    Haemophilia : the official journal of the World Federation of Hemophilia, 2020
    Co-Authors: C. E. Walsh, H. Chao
    Abstract:

    Gene therapy may revolutionize the treatment of haemophilia. Effective Gene therapy requires sustained therapeutic levels of factors IX (FIX) and VIII. Adeno-associated virus (AAV) is a member of the parvovirus family, is a nonpathogenic virus with a broad host cell range, and does not provoke a significant immune response upon infection. These favourable characteristics make AAV a suitable Gene Transfer vector for factor deficient patients. A new understanding of AAV biology coupled with novel AAV vector designs suggest that the goal of effective Gene Transfer is within reach. We review here recent advances in AAV vectors used for Gene Transfer of the haemophilias.

  • Parvovirus‐mediated Gene Transfer for the haemophilias
    Haemophilia, 2002
    Co-Authors: C. E. Walsh, H. Chao
    Abstract:

    Summary.  Gene therapy may revolutionize the treatment of haemophilia. Effective Gene therapy requires sustained therapeutic levels of factors IX (FIX) and VIII. Adeno-associated virus (AAV) is a member of the parvovirus family, is a nonpathogenic virus with a broad host cell range, and does not provoke a significant immune response upon infection. These favourable characteristics make AAV a suitable Gene Transfer vector for factor deficient patients. A new understanding of AAV biology coupled with novel AAV vector designs suggest that the goal of effective Gene Transfer is within reach. We review here recent advances in AAV vectors used for Gene Transfer of the haemophilias.

Hiroshi Tomogane - One of the best experts on this subject based on the ideXlab platform.

  • Gene Transfer to Mouse Embryos by Sperm Mediated Gene Transfer Method
    Journal of Applied Animal Research, 2006
    Co-Authors: Hiroki Furuta, Eri Ichikawa, Satoshi Sugimura, Sayaka Kikuchi, Tatsuyuki Yoshida, Harutaka Mukouyama, Hiroshi Tomogane
    Abstract:

    Abstract Furuta, H., Ichikawa, E., Sugimura, S., Kikuchi, S., Yoshida, T., Mukouyama, H. and Tomogane, H. 2006. Gene Transfer to mouse embryos by sperm mediated Gene Transfer method. J. Appl. Anim. Res., 29: 113–116. To obtain transgenic mouse embryos by in vitro fertilization transgenic mouse sperm were produced by electroporation using green fluorescent protein (GFP)Gene as a marker. The sperm was cultured in medium GFP Gene. Electoroporation was carried out under 200 V-1μF, 200V-25μF and 200V-50μF. The survival rate of sperm decreased by each treatment. Although the DNA Transfer into sperm mediated by electroporation was very easy, efficiency of introduction into embryos was very low. Sperm mediated Gene Transfer may be inferior to microinjection.

Maria Grazia Cerrito - One of the best experts on this subject based on the ideXlab platform.

  • Methods for sperm-mediated Gene Transfer
    Methods in Molecular Biology, 2013
    Co-Authors: Maria Luisa Lavitrano, Roberto Giovannoni, Maria Grazia Cerrito
    Abstract:

    The transgenic technologies represent potent biotechnological tools that allow the Generation of Genetically modified animals useful for basic research and for biomedical, veterinary, and agricultural applications. Among transgenic techniques, we describe here the sperm-mediated Gene Transfer methods that is Gene Transfer based on the spontaneous ability of sperm cells to bind and internalize exogenous DNA and to carry it to oocyte during fertilization, producing Genetically modified animals with high efficiency.