The Experts below are selected from a list of 34209 Experts worldwide ranked by ideXlab platform
Nynke Osinga - One of the best experts on this subject based on the ideXlab platform.
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Novel B19-like Parvovirus in the brain of a harbor seal
PloS one, 2013Co-Authors: Rogier Bodewes, Ana Rubio García, Marco W. G. Van De Bildt, Sarah Getu, Martijn Beukers, Claudia M. E. Schapendonk, Peter R. W. A. Van Run, Marjolein J. Poen, Lidewij Wiersma, Nynke OsingaAbstract:Using random PCR in combination with next-generation sequencing, a novel Parvovirus was detected in the brain of a young harbor seal (Phoca vitulina) with chronic non-suppurative meningo-encephalitis that was rehabilitated at the Seal Rehabilitation and Research Centre (SRRC) in the Netherlands. In addition, two novel viruses belonging to the family Anelloviridae were detected in the lungs of this animal. Phylogenetic analysis of the coding sequence of the novel Parvovirus, tentatively called Seal Parvovirus, indicated that this virus belonged to the genus Erythrovirus, to which human Parvovirus B19 also belongs. Although no other seals with similar signs were rehabilitated in SRRC in recent years, a prevalence study of tissues of seals from the same area collected in the period 2008-2012 indicated that the Seal Parvovirus has circulated in the harbor seal population at least since 2008. The presence of the Seal Parvovirus in the brain was confirmed by real-time PCR and in vitro replication. Using in situ hybridization, we showed for the first time that a Parvovirus of the genus Erythrovirus was present in the Virchow-Robin space and in cerebral parenchyma adjacent to the meninges. These findings showed that a Parvovirus of the genus Erythrovirus can be involved in central nervous system infection and inflammation, as has also been suspected but not proven for human Parvovirus B19 infection.
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Genome organization and phylogenetic analysis of Seal Parvovirus.
2013Co-Authors: Rogier Bodewes, Ana Rubio García, Marco W. G. Van De Bildt, Lidewij C. M. Wiersma, Sarah Getu, Martijn Beukers, Claudia M. E. Schapendonk, Peter R. W. A. Van Run, Marjolein J. Poen, Nynke OsingaAbstract:A. Genome organization of Seal Parvovirus. Indicated were the location of the major ORFs (grey) and the location of the start- and stopcodons on the nucleotide level counted from the 5’ end of the partial seal Parvovirus genome. B. Phylogenetic neighbor-joining tree with p-distance and 1,000 bootstrap replicates of the deduced amino acid sequences of the VP2 genes of various viruses of the subfamily Parvovirinae. Genbank accession numbers: Canine Parvovirus 2a: JQ996152, Porcine Parvovirus Tai’an: FJ853421, Mouse Parvovirus 2: NC_008186, Fox Parvovirus: KC692368, AMD (Aleutian Mink disease) Parvovirus: GU183264, Gray fox amdovirus: JN202450, Bufavirus-2 BF 39: JX027297, Human Parvovirus 4: AY622943, Swine Parvovirus H-1: AB076669, Bovine Parvovirus 3: AF406967. Seal Parvovirus: KF373759, Chipmunk Parvovirus: GQ200736, Pig tailed macaque Parvovirus: AF221123, Rhesus macaque Parvovirus: AF221122, Simian Parvovirus: U26342, Human Parvovirus B19: NC_000883, Muscovy duck Parvovirus: NC_006147, Adeno-associated virus-2: NC_001401, Porcine bocavirus 5: JN831651, Canine minute virus SH1: FJ899734, Human bocavirus 3: HM132056.
Charles F Timmons - One of the best experts on this subject based on the ideXlab platform.
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polymerase chain reaction amplification of archival material for Parvovirus b19 in children with transient erythroblastopenia of childhood
Pediatric Pathology & Laboratory Medicine, 1996Co-Authors: Beverly Barton Rogers, Zora R Rogers, Charles F TimmonsAbstract:The relationship between transient erythroblastopenia of childhood (TEC) and Parvovirus B19 infection remains uncertain, Large series using primarily serologic evaluation have not shown an association, whereas smaller series have reported Parvovirus B19 infection in such patients. Further, Parvovirus DNA or antigen has been detected in some patients seronegative for the virus at presentation. Polymerase chain reaction (PCR) amplification has never been used to evaluate patients with TEC for Parvovirus B19. We used the PCR in an attempt to detect Parvovirus B19 in DNA extracted from archived bone marrow coverslips of 16 patients diagnosed, with TEC. The patients ranged in age from 3 to 23 months and presented with a mean hemoglobin value of 5.4 g/dL. Sixty-nine percent were neutropenic and none was thrombocytopenic. None of the patients had histologic evidence of Parvovirus B19 infection in the bone marrow. DNA amplification for Parvovirus B19 was negative in each case. In contrast, Parvovirus B19 DNA was ...
H Cameron - One of the best experts on this subject based on the ideXlab platform.
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Audit of the management of women exposed to B19 Parvovirus in pregnancy
Archives of Disease in Childhood - Fetal and Neonatal Edition, 2010Co-Authors: G Thomas, H CameronAbstract:Introduction B19 Parvovirus causes a common childhood rash – ‘Slapped Cheek Syndrome’, however Parvovirus can also infect adults. Infection during pregnancy can cause serious complications for the fetus. In Sunderland there was a Parvovirus epidemic in 2008. Aim To examine the management of pregnant women in the Sunderland area exposed to Parvovirus from 2007 to 2008. Methods A complex filtration process was used for patient selection. It comprised as follows: The Parvovirus antibody screening log Sunderland Royal Hospital electronic system (HISS) Documentation in the maternity notes 23 standards were created. Results Sample size 103. 62% of women were immune to Parvovirus. 28% were susceptible. 10% were infected but had normal pregnancy outcome. 98% had no documentation of reassurance in their notes. 89% had no documentation of the date of exposure. 59% of those susceptible did not have 4-week follow-up sampling. Conclusion The current system of care of pregnant women who are susceptible to Parvovirus is ineffective. There is poor communication about their immune status and documentation of significant information, such as date of exposure, is incomplete. Recommendation (1) Health professionals should give priority to the care of pregnant women reporting exposure to Parvovirus. (2) The Antenatal Screening Midwife should coordinate the management of Parvovirus results. (3) A proforma-based care pathway should be established for pregnant women exposed to Parvovirus to improve documentation and management.
Rogier Bodewes - One of the best experts on this subject based on the ideXlab platform.
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Maximum likelihood tree of the VP2 gene and partial NS1 gene of SePV.
2014Co-Authors: Rogier Bodewes, Rebriarina Hapsari, Ana Rubio García, Guillermo Sánchez J. Contreras, Marco W. G. Van De Bildt, Miranda De Graaf, Thijs Kuiken, Albert D. M. E. OsterhausAbstract:Phylogenetic maximum-likelihood tree with 500 bootstrap replicates of the nucleotide sequence of the VP2 genes (A) and partial NS genes (B) of SePV variants and various viruses of the genus ErythroParvovirus. Only bootstrap values >70 are indicated. Genbank accession numbers: SePV-HG06130: KM252691, SePV-HG020628.02: KM252694, SePV-PV121216.01: KM252698, SePV-PV880823.6: KM252692, SePV-PV880927.20: KM252693, SePV12410: KF373759, SePV-PV020628.13: KM252695, SePV-PV020718.12: KM252696, SePV-PV020719.08: KM252697, bovine Parvovirus 3: AF406967, chipmunk Parvovirus: GQ200736, rhesus macaque Parvovirus: AF221122, simian Parvovirus: U26342, pig-tailed macaque Parvovirus: AF221123, human Parvovirus B19-Au: M13178, human Parvovirus B19-LaLi: AY044266, human Parvovirus B19-V9: AJ249437.
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Novel B19-like Parvovirus in the brain of a harbor seal
PloS one, 2013Co-Authors: Rogier Bodewes, Ana Rubio García, Marco W. G. Van De Bildt, Sarah Getu, Martijn Beukers, Claudia M. E. Schapendonk, Peter R. W. A. Van Run, Marjolein J. Poen, Lidewij Wiersma, Nynke OsingaAbstract:Using random PCR in combination with next-generation sequencing, a novel Parvovirus was detected in the brain of a young harbor seal (Phoca vitulina) with chronic non-suppurative meningo-encephalitis that was rehabilitated at the Seal Rehabilitation and Research Centre (SRRC) in the Netherlands. In addition, two novel viruses belonging to the family Anelloviridae were detected in the lungs of this animal. Phylogenetic analysis of the coding sequence of the novel Parvovirus, tentatively called Seal Parvovirus, indicated that this virus belonged to the genus Erythrovirus, to which human Parvovirus B19 also belongs. Although no other seals with similar signs were rehabilitated in SRRC in recent years, a prevalence study of tissues of seals from the same area collected in the period 2008-2012 indicated that the Seal Parvovirus has circulated in the harbor seal population at least since 2008. The presence of the Seal Parvovirus in the brain was confirmed by real-time PCR and in vitro replication. Using in situ hybridization, we showed for the first time that a Parvovirus of the genus Erythrovirus was present in the Virchow-Robin space and in cerebral parenchyma adjacent to the meninges. These findings showed that a Parvovirus of the genus Erythrovirus can be involved in central nervous system infection and inflammation, as has also been suspected but not proven for human Parvovirus B19 infection.
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Genome organization and phylogenetic analysis of Seal Parvovirus.
2013Co-Authors: Rogier Bodewes, Ana Rubio García, Marco W. G. Van De Bildt, Lidewij C. M. Wiersma, Sarah Getu, Martijn Beukers, Claudia M. E. Schapendonk, Peter R. W. A. Van Run, Marjolein J. Poen, Nynke OsingaAbstract:A. Genome organization of Seal Parvovirus. Indicated were the location of the major ORFs (grey) and the location of the start- and stopcodons on the nucleotide level counted from the 5’ end of the partial seal Parvovirus genome. B. Phylogenetic neighbor-joining tree with p-distance and 1,000 bootstrap replicates of the deduced amino acid sequences of the VP2 genes of various viruses of the subfamily Parvovirinae. Genbank accession numbers: Canine Parvovirus 2a: JQ996152, Porcine Parvovirus Tai’an: FJ853421, Mouse Parvovirus 2: NC_008186, Fox Parvovirus: KC692368, AMD (Aleutian Mink disease) Parvovirus: GU183264, Gray fox amdovirus: JN202450, Bufavirus-2 BF 39: JX027297, Human Parvovirus 4: AY622943, Swine Parvovirus H-1: AB076669, Bovine Parvovirus 3: AF406967. Seal Parvovirus: KF373759, Chipmunk Parvovirus: GQ200736, Pig tailed macaque Parvovirus: AF221123, Rhesus macaque Parvovirus: AF221122, Simian Parvovirus: U26342, Human Parvovirus B19: NC_000883, Muscovy duck Parvovirus: NC_006147, Adeno-associated virus-2: NC_001401, Porcine bocavirus 5: JN831651, Canine minute virus SH1: FJ899734, Human bocavirus 3: HM132056.
Beverly Barton Rogers - One of the best experts on this subject based on the ideXlab platform.
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polymerase chain reaction amplification of archival material for Parvovirus b19 in children with transient erythroblastopenia of childhood
Pediatric Pathology & Laboratory Medicine, 1996Co-Authors: Beverly Barton Rogers, Zora R Rogers, Charles F TimmonsAbstract:The relationship between transient erythroblastopenia of childhood (TEC) and Parvovirus B19 infection remains uncertain, Large series using primarily serologic evaluation have not shown an association, whereas smaller series have reported Parvovirus B19 infection in such patients. Further, Parvovirus DNA or antigen has been detected in some patients seronegative for the virus at presentation. Polymerase chain reaction (PCR) amplification has never been used to evaluate patients with TEC for Parvovirus B19. We used the PCR in an attempt to detect Parvovirus B19 in DNA extracted from archived bone marrow coverslips of 16 patients diagnosed, with TEC. The patients ranged in age from 3 to 23 months and presented with a mean hemoglobin value of 5.4 g/dL. Sixty-nine percent were neutropenic and none was thrombocytopenic. None of the patients had histologic evidence of Parvovirus B19 infection in the bone marrow. DNA amplification for Parvovirus B19 was negative in each case. In contrast, Parvovirus B19 DNA was ...
