The Experts below are selected from a list of 28956 Experts worldwide ranked by ideXlab platform
R.a. Woods - One of the best experts on this subject based on the ideXlab platform.
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Genetic Transformation of yeast
BioTechniques, 2001Co-Authors: Daniel R Gietz, R.a. WoodsAbstract:Genetic Transformation was first described by Griffith in 1928 and has since been demonstrated in a variety of organisms, including many species of fungi. This review focuses on the history and tec...
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Genetic Transformation of yeast
BioTechniques, 2001Co-Authors: R. Daniel Gietz, R.a. WoodsAbstract:Genetic Transformation was first described by Griffith in 1928 and has since been demonstrated in a variety of organisms, including many species of fungi. This review focuses on the history and technology of the Transformation of Saccharomyces cerevisiae. The application of protocols developed for S. cerevisiae to other important yeast species is discussed. The protocols for Transformation by spheroplasting, LiAc/ ssDNA/PEG, and electroporation are compared, and possible mechanisms for Transformation are discussed.
Armand Seguin - One of the best experts on this subject based on the ideXlab platform.
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recent advances in the Genetic Transformation of trees
Trends in Biotechnology, 2001Co-Authors: Leandro Pena, Armand SeguinAbstract:As the commercial production of transgenic annual crops becomes a reality in many parts of the world, many people wonder if the Genetic engineering of perennial trees will allow their eventual commercialization. Not long ago, trees were considered to be recalcitrant material for most molecular biology techniques, including Genetic Transformation. However, transgenes for shortening the juvenile phase or for phytoremediation purposes have now been incorporated, and the alteration of lignin biosynthesis and increased cellulose accumulation in forest trees have also been accomplished. For long-lived tree species, new questions arise regarding the stability of integration and expression of foreign genes. Biosafety considerations, including transgene dispersion through the pollen and advances in strategies to avoid this, are also important.
Vitaly Citovsky - One of the best experts on this subject based on the ideXlab platform.
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uncoupling of the functions of the arabidopsis vip1 protein in transient and stable plant Genetic Transformation by agrobacterium
Proceedings of the National Academy of Sciences of the United States of America, 2005Co-Authors: Jianxiong Li, Alexander Krichevsky, Manjusha Vaidya, Tzvi Tzfira, Vitaly CitovskyAbstract:Agrobacterium-mediated Genetic Transformation of plants, a unique example of transkingdom DNA transfer, requires the presence of several proteins encoded by the host cell. One such cellular factor is VIP1, an Arabidopsis protein proposed to interact with and facilitate import of the bacterial DNA–protein transport (T) complexes into the plant cell nucleus. Thus, VIP1 is required for transient expression of the bacterial DNA, an early step in the Transformation process. However, the role of VIP1 in subsequent Transformation events leading to the stable expression of bacterial DNA was unexplored. Here, we used reverse Genetics to dissect VIP1 functionally and demonstrate its involvement in the stable Genetic Transformation of Arabidopsis plants by Agrobacterium. Our data indicate that the ability of VIP1 to interact with the VirE2 protein component of the T-complex and localize to the cell nucleus is sufficient for transient Genetic Transformation, whereas its ability to form homomultimers and interact with the host cell H2A histone in planta is required for tumorigenesis and, by implication, stable Genetic Transformation.
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involvement of targeted proteolysis in plant Genetic Transformation by agrobacterium
Nature, 2004Co-Authors: Tzvi Tzfira, Manjusha Vaidya, Vitaly CitovskyAbstract:Genetic Transformation of plant cells by Agrobacterium represents a unique case of trans-kingdom DNA transfer1. During this process, Agrobacterium exports its transferred (T) DNA and several virulence (Vir) proteins into the host cell2, within which T-DNA nuclear import is mediated by VirD2 (ref. 3) and VirE2 (ref. 4) and their host cell interactors AtKAP-α5 and VIP1 (ref. 6), whereas its integration is mediated mainly by host cell proteins7,8,9. The factors involved in the uncoating of T-DNA from its cognate proteins, which occurs before integration into the host genome, are still unknown. Here, we report that VirF—one of the few known exported Vir proteins whose function in the host cell remains unknown—is involved in targeted proteolysis of VIP1 and VirE2. We show that VirF localizes to the plant cell nucleus and interacts with VIP1, a nuclear protein. VirF, which contains an F-box motif10, significantly destabilizes both VIP1 and VirE2 in yeast cells. Destabilization of VIP1 in the presence of VirF was then confirmed in planta. These results suggest that VIP1 and its cognate VirE2 are specifically targeted by the VirF-containing Skp1–Cdc53-cullin–F-box complex for proteolysis. The critical role of proteasomal degradation in Agrobacterium-mediated Genetic Transformation was also evident from inhibition of T-DNA expression by a proteasomal inhibitor.
Yaxin Ge - One of the best experts on this subject based on the ideXlab platform.
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Recent advances in Genetic Transformation of forage and turf grasses
In Vitro Cellular & Developmental Biology - Plant, 2006Co-Authors: Zeng-yu Wang, Yaxin GeAbstract:Forage and turf grasses are critical to sustainable agriculture and contribute extensively to the world economy. Tremendous progress has been made in Genetic Transformation of forage and turf grasses in the past decade. The rapid advancement of cellular and molecular biology and transgenic technology provides novel methods to accelerate and complement conventional breeding efforts. This review summarizes the latest developments in Genetic Transformation methods and the applications of molecular techniques for the improvement of forage and turf grasses.
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invited review recent advances in Genetic Transformation of forage and turf grasses
In Vitro Cellular & Developmental Biology – Plant, 2006Co-Authors: Zeng-yu Wang, Yaxin GeAbstract:Forage and turf grasses are critical to sustainable agriculture and contribute extensively to the world economy. Tremendous progress has been made in Genetic Transformation of forage and turf grasses in the past decade. The rapid advancement of cellular and molecular biology and transgenic technology provides novel methods to accelerate and complement conventional breeding efforts. This review summarizes the latest developments in Genetic Transformation methods and the applications of molecular techniques for the improvement of forage and turf grasses.
Leandro Pena - One of the best experts on this subject based on the ideXlab platform.
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recent advances in the Genetic Transformation of trees
Trends in Biotechnology, 2001Co-Authors: Leandro Pena, Armand SeguinAbstract:As the commercial production of transgenic annual crops becomes a reality in many parts of the world, many people wonder if the Genetic engineering of perennial trees will allow their eventual commercialization. Not long ago, trees were considered to be recalcitrant material for most molecular biology techniques, including Genetic Transformation. However, transgenes for shortening the juvenile phase or for phytoremediation purposes have now been incorporated, and the alteration of lignin biosynthesis and increased cellulose accumulation in forest trees have also been accomplished. For long-lived tree species, new questions arise regarding the stability of integration and expression of foreign genes. Biosafety considerations, including transgene dispersion through the pollen and advances in strategies to avoid this, are also important.